• FLOWER RESEARCH JOURNAL
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• v.17 no.1
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• pp.1-8
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• 2009

This study was conducted to investigate the efficiency of water quality remediation and the inorganic ion removal characteristics by floating plant; water lettuce (Pistia stratiotes L.) in a batch reactor. Water lettuce can be used to remove N and P and other inorganic nutrients, by consuming them in the form of plant nutrient. The highest nutrient absorption of water lettuce was $112.5meL^{-1}\;N$ in Sonneveld-2S, $56.6meL^{-1}\;N$ in Sonneveld-1S, $31.8meL^{-1}\;N$ in sewage and P value was also the highest in Sonneveld-2S as $15.6meL^{-1}$ and in sewage as $5.0meL^{-1}$. These results indicated that using water lettuce held some promise in the context of purification of eutrophication. Also water lettuce had a preference for absorption N. Under Sonneveld-2S treatment, nitrogen percentage was the highest in plant tissue due to the highest concentration of nitrogen and removal by water lettuce. Under sewage, percentage of total N in both plant parts was high. Both above and underground parts, P percentage was less than N. It might be due to the fact that the absorption amount of P was less than N by water lettuce. It meaned that the ability of N removal by water lettuce was higher than that of P, relatively. In summary, these results show that the use of this plant was a viable option.

• Journal of Sericultural and Entomological Science
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• v.28 no.1
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• pp.1-14
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• 1986

This study was carried out to acguire some basic information on nutritional and lhysiological effects of vesicular arbuscular mycorrhizae(VAM) on mulberry trees inoculated with Glomus mosseae, Gerd. & Trap. grown in clay for 65 days and treated with 5 different levels of phosphorus, ie 30, 60, 120, 240, 480 ppm as (NH4)2 HPO4. At the End of the expermental period the levels of fixed phosphate in the soil was measared. And the native VAM fungi were collected to select the most effective VAM species on mulberry tree. The nutritional and biochemical effects of VA mycorrhizae on the mulberry leaves were also studied. Those results are as follow. 1. The mulberry trees grown in clay and inoculated with VAM were heavier in shoot dry weight as much as 197% than uninoculated plants. But in vermioulite, uninoculated mulberry trees were heavier as much as 135% than inoculated. 2. The rates of endo mycorrhizal formation in clay was highest at 60 ppm level of phosphorus, and vesicles in roots were formed in 240ppm and 480ppm level of phosphorus, but not in 30ppm, 60ppm and 120ppm. 3. The greatest growth responses of VAM inoculation was found at 60ppm level of hosphorus, and the optimum phosphorus level for VAM responese appeared to be 60ppm. 4. VAM was also to absorb soil-fixed phosphate. VAM abosrbed Fe-bound phosphate most efficiently and Ca-bound phosphate with ease but not Al-bound phosphate and Al-Fe occluded phosphate. 5. Three species of Gigaspora and one species of Glamus growing naturally in mulberry plantations were collected and tested for the growth responses. Gigaspora tricalipta and Gigaspora calospora revealed the greatest growth responses on mulberry tree among tested VAM fungi. 6. Mulberry leaves inoculated with VA mycorrhizal fungi contained 9.8% more phosphate and 15.2% more nitrogen, 22.2% more water-soluble carbohydrates and 15.2% more proteins than uninoculated plants. 7. The electrophoretic pattern of mulberry leaf protein inoculated with VAM fungi has 19 bands. 8. The patterns of peroxidase zymogram and Amulase zymogram were different between the mulberry leaves inoculated and uninoculated with VA mycorrhizal fungi. The peroxidase zymogram of inoculated leaves has 1 less majour band than unioculated leaves The amylase zymogram of inoculated leaves has 2 bands near the +pole, but that of uninoculated leaves has 1 band near the $pole.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.5
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• pp.853-858
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• 2011

The cultivation methods are important for determining crop yield of green manure. The effect of cultivation methods of green manure crops (GMC) on biomass and rice yield was investigated. This experiment was conducted at Sinheung series (fine loamy, mixed, nonacid, mesic family of Fluvaquentic Endoaquepts from Oct. 2007 to Oct. 2008 at the National Institute of Crop Science (NICS), RDA, Suwon, Gyeonggi province, Korea. Seven GMC (hairy vetch, barley, Chinese milk vetch, rye, crimson clover, oats, rattail fescue) were cultivated and incorporated on paddy soil by broadcasting before rice harvesting (BBRH) and partial tillage seeding (PTS). Among the three leguminous GMC, the biomass and N production were the highest at the hairy vetch of PTS. Among the four gramineae GMC, the biomass and N production tended to be higher in the rye of BBRH and barley of PTS. The C/N ratio (56.5~74.2) of rye was high compared with hairy vetch (14.1). Among the GMC, the incorporation of hairy vetch increased $NH_4$-N contents in rice paddy soil at 14 and 42 days after transplanting. These results showed that hairy vetch had no significant to rice yield compared with conventional fertilization. Therefore, hairy vetch seems to be the most efficient green manure crop as an alternatives to chemical N fertilizer in the central regions of Korea.

• Korean Journal of Ecology and Environment
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• v.44 no.1
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• pp.9-21
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• 2011

This study is conducted to know the change in water environment of Lake Hwajinpo from 2000 to 2008 with physico-chemical parameters; salinity, dissolved oxygen, total phosphorus and total nitrogen and others. And zooplanktons and phytoplanktons were studied from 2007 to 2008. From the water quality data of Lake Hwajinpo from 2000 to 200S; water temperature, salinity, transparency, chemical oxygen demand and dissolved oxygen ranges are $2.8{\sim}29.4^{\circ}C$, 0.23~33.2‰, $0.2{\sim}1.8\;m$, $0.2{\sim}20.2\;mg\;L^{-1}$ and $0.1{\sim}17.4\;mg\;L^{-1}$ and the average values are $18.0^{\circ}C$, 15.7‰, 0.7 m, $5.7\;mg\;L^{-1}$ and $8.0\;mg\;L^{-1}$, respectively. Total phosphorus (TP) and total nitrogen (TN) ranges are $0.024{\sim}0.869\;mg\;L^{-1}$ (average 0.091) and $0.240{\sim}5.310\;mg\;L^{-1}$ (average 1.235). Average TN/TP ratio is 16.4. The annual variations in COD, TP, TN and Chl.${\alpha}$ are compared. COD in 2000 is $4.83\;mg\;L^{-1}$ and 2008 is $1.80\;mg\;L^{-1}$ which is reduced by $0.34\;mg\;L^{-1}$ every year. TP in 2000 is $0.07\;mg\;L^{-1}$ and 2008 is $0.05\;mg\;L^{-1}$ reduced gradually. Yearly reduction in TN is $0.09\;mg\;L^{-1}$, in 2000 and 2008 the values are $1.54\;mg\;L^{-1}$ and $0.77\;mg\;L^{-1}$ respectivly. Chl.${\alpha}$ in 2000 is $46.30\;{\mu}g\;L^{-1}$ and $5.78\;{\mu}g\;L^{-1}$ in 2008; yearly reduction is $4.50\;{\mu}g\;L^{-1}$. The tropic state index (TSI) in south and north parts of Lake Hwajinpo in 2000 are 67 and 63 which are reduced to 63 and 59 in 2008 respectively. North and south part of Lake Hwajinpo have 67 species of phytoplankton under 47 families in 2007 and 2008. Dominant species in south part in 2007 are; Asterococcus superbus in May, Lyngbya sp. in September and Trachelomonas spp. in November and in 2008 Anabaena spiroides in August are abundant and varies with time. Zooplankton species in Lake Hwajinpo are 25 of 25 families. Dominant species in south part in May and August 2007 and May and November in 2008 Copepoda larvae and in September 2007 Protozoa spp. of Protozoan and Brachionus plicatilis and Brachionus urceolaris of Cladocera in August 2008. Dominant species in north part Asplanchna sp. of Cladecera in August and November 2007 and rest of the time are larvae of Copepoda. In this way, the water quality of Lake Hwajinpo is changing with slow rate in the long period specially nutrients concentration (TP, TN etc) is decreasing.

• Applied Biological Chemistry
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• v.18 no.1
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• pp.16-22
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• 1975

This experiment was carried out to investigate the physiological characteristics of two original yeasts, 5-Y-5 and 6-Y-6, which selected from 24 Takju yeasts and three mutants, 30-24,30-81 and 40-27. induced from two original yeasts by the irradiation of UV light. The results were summarized as follows. 1) Alcohol tolerances of three mutants were decreased in some degree as compared with those of original yeasts. 2) Tolerances of lactic and citric acids of acid producing mutant 30-81, was increased than those of original yeasts. 3) In the case of using ammonium sulfate as a nitrogen source, two original yeasts and three mutants required Ca-pantothenate as a essential growth factor and four strains of yeasts except the mutant, 30-81, required biotin as a stimulated growth factor, When asparagine was used as a nitrogen source, two original yeasts and three mutants showed the same as above result but the stimulated effect of biotin was far less. 4) Propagation powers of the mutants were weaken than those of original yeasts, particular that of acid producing mutant, 30-81, was the weakest in the three mutants. 5) The optimum temperature for fermentation of original yeasts were $30^{\circ}C\;to\;35^{\circ}C$ but three mutants were $25^{\circ}C\;to\;30^{\circ}C$. 6) The optimum pH for fermentation of original yeasts were pH 5 to 6, and there is no appreciable difference between original yeasts and three mutants. The fermentation power of mutant,30-81, was decreased more rapidly than those of other mutants according to approach neutral. Three mutants were more sensible to heat than original yeasts. 7) Two original yeasts and three mutants were inhibited more over 20 percent of sugar for fermentation and three mutants were more sensible to sugar concentration than original yeasts.

• Korean Journal of Food Science and Technology
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• v.45 no.5
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• pp.537-544
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• 2013

This study was performed to establish method of simultaneous determination of fructose, glucose, and sucrose in honey and commercial vitamin drinks by GC and GC/MS. Optimum chromatographic separation of trimethylsilyl-oxime (TMSO) derivatives by GC was achieved on a DB-5 column. Calibration curves for fructose, glucose and sucrose TMSO derivatives by GC were linear in the range of 50-5000 ${\mu}g/mL$, and their $r^2$ values were 0.9999, The limit of detection and limit of quantification of fructose, glucose, and sucrose were 0.68, 0.47, and 0.53 ${\mu}g/mL$, respectively, and 2.27, 1.58, and 1.77 ${\mu}g/mL$, respectively. Average recoveries of fructose, glucose, and sucrose were 100.5, 101.0, and 99.7%, respectively. When the method was applied to 12 honey samples, the average concentrations of fructose, glucose and sucrose were $42.58{\pm}1.97%$, $27.74{\pm}1.16%$, and $0.79{\pm}0.52%$, respectively. The F/G ratio was $1.53{\pm}0.07$. For fructose and glucose contents, results from the GC analysis were similar to those from the HPLC analysis, but the sucrose content was different for each analysis method. We suggest that the GC method is more suitable than other official analytical methods for simultaneous determination of fructose, glucose, and sucrose in honey.

• Journal of The Korean Society of Grassland and Forage Science
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• v.36 no.4
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• pp.309-317
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• 2016

The study was conducted to evaluate the effects of microbial culture supplements on ruminal fermentation and fermentative quality of Italian ryegrass silage (IRGS) both in vitro and in situ. Three species of microbes (Lactobacillus casei (LC), Bacillus subtilis (BS), and Saccharomyces cerevisiae (SC)) were used in this study. They were applied to IRGS at 30 days after silage manufacture. Various items were measured using in vitro and in situ incubation technique after each microbial supplement was inoculated into IRGS at $0.5{\times}10^4CFU/g$. In the first experiment, in vitro ruminal fermentation characteristics of IRGS were evaluated at 0, 12, 24, 48, and 72 hours after microbes were inoculated into IRGS. In the second experiment, in situ fermentation characteristics were investigated at 0, 1, 3, and 5 days after the inoculation of each microbial supplement. In vitro ruminal $NH_3-N$ content was significantly (p<0.05) increased in LC-, BS-, and SC-IRGS at 12 hrs post incubation compared to that in control IRGS. In vitro ruminal total VFA concentration and dry matter digestibility (DMD) of IRGS were not significantly difference among LC-, BS-, and SC-IRGS, although they were numerically increased in LC-IRGS than those of the other IRGS. In addition, this study evaluated the fermentation characteristics and in situ DMD of IRGS with the lapse of incubation time up to 5 days. Throughout the incubation times from 1 day to 5 days, the pH value was significantly (p<0.05) lower in BS-, LC-, and SC-IRGS than that in control IRGS. Lactate was significantly (p<0.05) higher, and significantly (p<0.05) butyrate was lower in LC-IRGS than that in other treatments at 0 day. It was higher (p<0.05) in control IRGS than that of BS-, LC-, and SC-IRGS at 1-5 days. In situ DMD tended to increase in BS-, LC-, and SC-IRGS compared to that in control IRGS. Especially, DMD was higher in SC-IRGS than that in other treatments at 0 day. It tended to be higher in LC-IRGS at all incubation time. Taken together, these results suggest that it might be useful to select a microorganism by considering the feeding time of IRGS to ruminants because organic acids and DMD of IRGS were affected by the incubation time of each microorganism with IRG silage, especially for L. casei decreased the content of acetate and butyrate in IRGS.

• Korean Journal of Poultry Science
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• v.42 no.1
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• pp.15-26
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• 2015

In this study, genotyping was executed by using 27 microsatellite markers for genetic diversity of 469 Korean Native Chickens [20 population, each population is 24 samples but Hanhyup A line is 13 samples). in total 469 samples were collected from National Institute of Animal Science (Korean Native Chicken (NR, NY, NG, NL and NW), Ogye (NO), Leghorn F,K (NF and NK), Black and Brown cormish (NH and NS), Rhode Island Red C, D (NC and ND), Total is 12 populations] and Hanhyup [H line (HH), F line (HF), G line (HG), V line (HV), S line (HS), W line (HW), Y line (HY), A line (HA), total is 8 populations]. [The allele number were observed 5 (ADL0268) to 20 (MCW0127) each markers. Observed heterozygostiy ($H_{obs}$), expected heterozygosity ($H_{exp}$), polymorphism Information Content (PIC) were observed 0.359 to 0.677, 0.668 to 0.881 and 0.646 to 0.869, respectively. Using these markers, the calculated the heterozygote deficit within chicken line ($F_{is}$) value each population from mean 0.117. Phylogenetic tree showing the genetic relationship among 20 population using standard genetic distance calculated from 27 microsatellite markers. genetic distances revealed the closest (0.175) between NC and ND. on the other hand, Farthest genetic distances (0.710) revealed between NF and HV. STRUCTURE analysis and Principal Components Analysis (PCA) showed that results of similar phylogenetic tree. The expected probability of identity values on random individuals (Total population and only Hanhyup line) was estimated at $8.80{\times}10^{-83}$ and $3.87{\times}10^{-117}$, respectively. In conclusion, This study shows the useful data that be utilized as a basic data of Korean Native Chicken breeding and development for commercial chicken industry to meet the consumer's demand.

• Korean Journal of Soil Science and Fertilizer
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• v.9 no.2
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• pp.99-105
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• 1976

After investigation of soil characteristics the properties of a reclaimed marine soil (Gupo series) distributed along the southern coastal areas of Korea are summarized as follows: 1. Gupo soils distributed in the southern Ria coastal area are derived from rolling to hilly materials and are poorly sorted with less influences of river fluvial action. These soils have high content of sand compared with the broad fluvio-marine soils in the western coastal areas. 2. The morphological features of the poorly drained Gupo soils are greyish brown sandy loam with a few yellowish mottles in the surface horizon and are grey sandy loam with a few gravel in the sub-strata. The ground water table remains around 10-30cm below the surface. These soils, recently reclaimed younger deposits, do not show any evidence of illuviation. 3. The "n" value (about 0.8) of the Gupo soils indicates physically unripened soils. 4. pH value of these soils shows more than 8.0 throughout the profile. Organic matter contents are extremely low (around 0.5%) except 1.2 percent in the surface horizon. C.E.C. ranges from 7 to 9m.e/100g which is lower than average in the country. The ratios of extractable cations such as Ca, Mg, Na and K of the surface horizon are 20:7:4:1. Base saturation is more than 60%. Available phosphate content is very low that is less than 25 ppm. Electric conductivity of the soils at $25^{\circ}C$ ranges 7 to 12 mmhos/cm and increased with depth. 5. According to classification of soil based on physical ripening, the Gupo soils can be classified into "Unripe soils with half-ripe sub-soils". The soils could be classified into "Hydric Haplaquents" in the original of the 7th Approximation (1960), but into "Typic Haplaqents in the supplement of 7th approximation which the physical ripening condition is not clearly expressed. Soil Taxonomy, apparently the final version of the 7th approximation, defines the soils as "Haplic Hydraquents" that clearly show the condition of physical ripening as well as other properties. Other several classification systems applied do not describe physical ripening condition of the soils.

• Korean Journal of Soil Science and Fertilizer
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• v.25 no.4
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• pp.325-333
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• 1992

An investigation was carried out to find out the effects of long-term application (14 and 28 years) of rice straw, compost and wheat straw on changes in soil chemical and physical properities, aspests of releasing potential nitrogen and nitrogen uptake by rice and maize from fine textured paddy soils with double cropping system in warm temperate area. The result obtained were summarized as follows : 1. The long-term application of organic matters improved plow layer and soil physical properties : bulk density and solid phase were decreased, while porosity and gaseous phase were decreased. 2. Average increment of total carbon per year was 0.0371% and 0.0407% for rice straw and compost, respectively, from 1 through 14 years ; it was 0.0007% and 0.0014% for the rice straw and compost, respectively, from 15 years through 28 years. The average increment of total notrogen per year was 0.0025% and 0.038% for the rice straw and compost, respectively, from 1 through 14 years ; 0.0014% and 0.0024% for the same treatments from 15 through 28 years. 3. $NH_4-N$ and amide-N were high in the soils with wheat straw application for 28 years ; the amino sugar-N in the soils with compost application for 28 years ; amino acid -N in the soils with rice straw application for 14 and 28 years ; and unidentified-N, in the control. 4. The released amount of available nitrogen with the submerged condition was higher at $30^{\circ}C$ than at $25^{\circ}C$ during the incubation. The amount of released available nitrogen at the field was aproximately same as that of $25^{\circ}C$ incubation. However, the released amounts from the incubation and the field were always lower than those extracted with reagents. 5. The amount of nitrogen uptake by rice and maize was highly correlated with available nitrogen extracted with phosphate buffer(pH 7.0). 6. The ratio of yield increase(milled rice) was 17, 12 and 7%, respectively, by application of rice straw, compost and wheat straw for 28 years, and 11% by application of rice straw for 14 years.