• Animal cells and systems
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• v.16 no.4
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• pp.295-301
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• 2012

Interdigital tissue regression is one of the most well-known examples of embryonic programmed cell death, providing the mechanism behind separation of developing digits. Caspases have been shown to play a key part in this process, with activated caspase-3 localized between the developing digits. In caspase-3 knock-out adult mice, however, the digits are completely separated with no webbing. In other mutants with defects in the apoptotic machinery, such as Apaf1 deficient mice, interdigital tissue regression is initially inhibited but the webbing eventually disappears as alternative/additional cell death mechanisms step in. In order to investigate whether a similar temporal effect occurs after loss of caspase-3, we have used an in vitro approach to inhibit caspase-3 at specific times during digit separation. Previous limb explant culture approaches have encountered problems with proper limb development in culture, and thus a modified technique was used. The new approach enables detailed observation of the effects of caspase-3 inhibition on interdigital regression. Using these methods, we show that caspase-3 inhibition caused a delay in the loss of interdigital tissue compared with control explants, similar to that observed in Apaf1 mutant mice. Along with immunohistochemistry, active caspase-3 positive cells of the interdigital vs. digital regions were measured by flow cytometry. Notably, activated caspase-3 in vivo was found not only in the interdigital mesenchyme but also in the TUNEL negative digit region, supporting a role for caspase-3 in nonapoptotic events.

• Environmental Engineering Research
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• v.22 no.4
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• pp.407-416
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• 2017

$CO_2$ emission is increasingly focused by public. Beijing and Tianjin are conceived to be a new economic point of growth in China. However, both of them are suffering serious environmental stress. In order to seek for the effect of socioeconomic factors on the $CO_2$ emission of this region, a novel methodology -symbolic regression- is adopted to investigate the relationship between $CO_2$ emission and influential factors of Beijing and Tianjin. Based on this method, $CO_2$ emission models of Beijing and Tianjin are built respectively. The models results manifested that Beijing and Tianjin own different $CO_2$ emission indicators. The RMSE of models in Beijing and Tianjin are 255.39 and 603.99, respectively. Further analysis on indicators and forecast trend shows that $CO_2$ emission of Beijing expresses an inverted-U shaped curve, whilst Tianjin owns a monotonically increasing trend. From analytical results, it could be argued that the diversity rooted in different development orientation and the mixture of different natural and industrial environment. This research further expands the investigation on $CO_2$ emission of Beijing and Tianjin region, and can be used for reference in the study of carbon emissions in similar regions. Based on the investigation, several policy suggestions are presented.

• STI Policy Review
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• v.7 no.2
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• pp.106-131
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• 2016

The general purpose of this paper is to identify opportunities for and to measure existing collaboration on research and development between institutions from the countries of Asia and Latin America in FEALAC's framework, in the fields of biotechnology and nanotechnology and their convergence. The methodological approach includes scientific and technological surveillance and research seeking to identify both the R&D and innovation capacities of the countries as well as the degree of international cooperation between countries of the two regions; case studies and a study of the governance framework of international collaboration in R&D about issues considered global challenges. The study has three main findings. First, nanotechnology, biotechnology and their convergence contribute to solving the problem of contamination by heavy metals affecting most of the countries that are part of FEALAC and to address problems arising from the accelerated rate of energy consumption, which also contributes to environmental damage. In this scenario, important business opportunities arise from the adaptation and development of bio-refinery technologies. Second, the scientific relationship between FEALAC countries, mainly between Asian and Latin American countries, is weak as can be seen in research for articles and patents. But there is plenty of room and potential for improvement. Third, current and upcoming joint R&D programs and projects should be linked both to existing governance structures and to new ones that serve as experiments of STI public policy regarding innovative management of intellectual property and capacity building. Practical implications are included in lessons learned and a set of recommendations involving a couple of proposals. One proposal calls for research and innovation in promising fields for international cooperation. Another proposal creates mechanisms in the governance framework for sharing knowledge, capacity building, and funding.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.4
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• pp.427-432
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• 2009

Seashore Paspalum (Paspalum vaginatum Swartz) is a warm season grass and indigenous to tropical and subtropical regions of coastal areas worldwide. The species is used as feed for cattle and horses and has been very successful for golf courses worldwide. One of the most outstanding characteristics of seashore paspalum is its tolerance to saline soils compared to other warm season turfgrasses. The development of new seashore paspalum cultivars with improved traits could be facilitated through the application of biotechnological strategies. The purpose of this study was to product for herbicide resistant seashore paspalum using Arobacterium-mediated transformation and this study is the first report on transformation and herbicideresistant transgenic plants in seashore paspalum. Embryogenic calli were induced from the seeded variety of pseashore paspalum. Embryogenic calli were transformed with Agrobacterium tumefaciens strain EHA105 carrying the binary vector pCAMBIA3301 with two genes encoding gusA and bar. Transformed calli and plants were selected on medium containing 3 mg/l PPT. PCR detected the presence of the gusA and bar gene, indicating both genes are integrated into the genome of seashore paspalum. A chlorophenol red assay was used to confirm that the bar gene was expressed. By application of herbicide BASTA, the herbicide resistance in the transgenic seashore paspalum plants was confirmed.

• Structural Engineering and Mechanics
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• v.51 no.5
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• pp.755-771
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• 2014

Environmental changes, especially global climate change, are creating new challenges to the development of the Arctic regions, which have substantial energy resources. And attention to offshore structures has increased with oil and gas development. The structural impact response of an explosion-resistant profiled blast walls normally changes when it operates in low temperatures. The main objectives of this study are to investigate the structural response of blast walls in low temperature and suggest useful guidelines for understanding the characteristics of the structural impact response of blast walls subjected to hydrocarbon explosions in Arctic conditions. The target temperatures were based on the average summer temperature ($-20^{\circ}C$), the average winter temperature ($-40^{\circ}C$) and the coldest temperature recorded (approximately $-68^{\circ}C$) in the Arctic. The nonlinear finite element analysis was performed to design an explosion-resistant profiled blast wall for use in Arctic conditions based on the behaviour of material properties at low temperatures established by performing a tensile test. The conclusions and implications of the findings are discussed.

• Journal of Korea Trade
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• v.25 no.2
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• pp.95-110
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• 2021

Purpose - This paper intends to conduct theoretical analysis and empirical test on the action mechanism of South Korea-China trade and South Korea's FDI to China on green total factor productivity, so as to provide a new perspective and ideas for the improvement of China's green total factor productivity and promote the high-quality development of China's economy Design/methodology - This paper uses the data of 30 provinces, autonomous regions and municipalities in China from 2004 to 2017 as the research sample, adopts the GML index method of SBM Directional Distance Function to measure GTFP, and analyzes the influence of South Korea-China trade and FDI from South Korea on China's GTFP. Findings - Trade is conducive to promoting technological progress, which has a significant promotion effect on China's green total factor productivity. While FDI has a significant inhibitory effect on China's green total factor productivity, which verifies the "pollution haven" hypothesis. In addition, such influence has certain regional overall heterogeneity. Trade has a more significant promoting effect on GTFP in eastern coastal areas, while FDI has a more significant inhibitory effect on GTFP in central and western inland areas. The interaction between trade and FDI is conducive to the improvement of green total factor productivity, indicating that the benign mechanism of trade and FDI has been formed. Urbanization, industrial structure, human resource level and investment in science and technology are all conducive to the improvement of GTFP. Originality/value - Through theoretical analysis and empirical test on the action mechanism of South Korea-China trade and South Korea's FDI on green total factor productivity, this paper provides a solid theoretical foundation for the further development of China-South Korea economic and trade cooperation in the future.

• Korean Journal of Medicinal Crop Science
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• v.25 no.6
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• pp.411-417
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• 2017

Background: Adenophora triphylla var. japonica (Regel) H. Hara shows vegetative growth with radical leaves during the first year and shows reproductive growth with cauline leaves and bolting during the second year. In addition, the shape of the plant varies within the same species. For this reason, there are limitations to classifying the species by visual examination. However, there is not sufficient genetic information or molecular tools to analyze the genetic diversity of the plant. Methods and Results: Approximately 34.59 Gbp of raw data containing 342,487,502 reads was obtained from next generation sequencing (NGS) and these reads were assembled into 357,211 scaffolds. A total of 84,106 simple sequence repeat (SSR) regions were identified and 14,133 primer sets were designed. From the designed primer sets, 95 were randomly selected and were applied to the genomic DNA which was extracted from five plants and pooled. Thirty-nine primer sets showing more than two bands were finally selected as SSR markers, and were used for the genetic relationship analysis. Conclusions: The 39 novel SSR markers developed in this study could be used for the genetic diversity analysis, variety identification, new variety development and molecular breeding of A. triphylla.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.41 no.4
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• pp.34-41
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• 2018

This paper studied balanced regional development focused on employment in Korea, by analyzing regional disparity between regional and industrial employment. A Gini-coefficient decomposition method and Panel Granger causality test were conducted, using raw data of the Census on Establishments reported by the Statistics Korea. The regional and industrial disparity of employment, based on the Gini-coefficient decomposition method, have increased by year. However, the growth rates of disparity are on the decrease. Most of employment disparity occurred from regional disparity between SMA (Seoul Metropolitan Area) and Non-SMA. Industrial disparity are occurred mainly by the service industry. The amount of contribution to the whole disparity of inter/intra regional employment was differed by each industrial sector. Also the causal relationship between employment growth of manufacture and that of service industry was analyzed by Panel Granger causality test. In national level, the employment growth in manufacture industry has conduced that in service industry. On the other hand, in the Non-SMA, only the employment growth in manufacture industry has augmented that in service industry. In conclusion, to reduce employment disparity, the strategy for balanced regional development should be emphasized. Different strategies are needed across regions and industries. Basically creating new job in the Non-SMA is inevitable. In view of stable employment, manufacture industry is more desirable rather than service industry.

• Journal of Korean Society of Transportation
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• v.22 no.4 s.75
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• pp.117-134
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• 2004

Truck traffic accounts for a substantial fraction of the traffic stream in many regions and is often the source of localized traffic congestion, potential parking and safety problems. Truck trips tend to be ignored or treated superficially in travel demand models. It reduces the effectiveness and accuracy of travel demand forecasting and may result in misguided transportation policy and project decisions. This paper presents the development of speed-flow relationships with truck impacts based on CORSIM simulation results in order to enhance travel demand model by incorporating truck trips. The traditional BPR(Bureau of Public Road) function representing the speed-flow relationships for roadway facilities is modified to specifically include the impacts of truck traffics. A number of new speed-flow functions have been developed based on CORSIM simulation results for freeways and urban arterials.

• The Journal of the Korea Contents Association
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• v.11 no.5
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• pp.466-473
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• 2011

The purpose of this study is to analyze the distribution of the building area and find the characteristics of development density where the areas to perform special functions as residential, commercial, and business area in Seoul. As a result of classification of the regions with the component ratio of building area ratio by types, the greater part of Seoul is residential areas and only a small part of Seoul have business function. Contrary to the Kang-Nam business districts, the central business districts have few or no houses. It is concerned that the redevelopment projects, such as New-Town, make Seoul more high- density. The industrial area lost the attractiveness as a resident in spite of businesses or infrastructures. So the industrial area will be the best place for reduce journey-to-work distance if improve housing conditions. As well as management of density, management of use is also required for effective landuse and green-growth city.