• Title/Summary/Keyword: NES

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Differential gene expression profiles of periodontal soft tissue from rat teeth after immediate and delayed replantation: a pilot study

  • Chae, Yong Kwon;Shin, Seo Young;Kang, Sang Wook;Choi, Sung Chul;Nam, Ok Hyung
    • Journal of Periodontal and Implant Science
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    • v.52 no.2
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    • pp.127-140
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    • 2022
  • Purpose: In dental avulsion, delayed replantation usually has an uncertain prognosis. After tooth replantation, complex inflammatory responses promote a return to periodontal tissue homeostasis. Various types of cytokines are produced in the inflammatory microenvironment, and these cytokines determine the periodontal tissue response. This study aimed to identify the gene expression profiles of replanted teeth and evaluate the functional differences between immediate and delayed replantation. Methods: Maxillary molars from Sprague-Dawley rats were extracted, exposed to a dry environment, and then replanted. The animals were divided into 2 groups according to the extra-oral time: immediate replantation (dry for 5 minutes) and delayed replantation (dry for 60 minutes). Either 3 or 7 days after replantation, the animals were sacrificed. Periodontal soft tissues were harvested for mRNA sequencing. Hallmark gene set enrichment analysis was performed to predict the function of gene-gene interactions. The normalized enrichment score (NES) was calculated to determine functional differences. Results: The hallmark gene sets enriched in delayed replantation at 3 days were oxidative phosphorylation (NES=2.82, Q<0.001) and tumor necrosis factor-alpha (TNF-α) signaling via the nuclear factor kappa light chain enhancer of activated B cells (NF-κB) pathway (NES=1.52, Q=0.034). At 7 days after delayed replantation, TNF-α signaling via the NF-κB pathway (NES=-1.82, Q=0.002), angiogenesis (NES=-1.66, Q=0.01), and the transforming growth factor-beta signaling pathway (NES=-1.46, Q=0.051) were negatively highlighted. Conclusions: Differentially expressed gene profiles were significantly different between immediate and delayed replantation. TNF-α signaling via the NF-κB pathway was marked during the healing process. However, the enrichment score of this pathway changed in a time-dependent manner between immediate and delayed replantation.

Cloning of nif genes from Enterobacter agglomerans in Escherichia coli. (Enterobacter agglomerans의 질소고정유전자 Cloning)

  • 정건섭;이정기;민태익;변유량;유주현
    • Microbiology and Biotechnology Letters
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    • v.15 no.2
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    • pp.116-121
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    • 1987
  • In order to cloning of the nif genes of Enterobacter agglomerans NFB-264, the digested total DNA of the strain was ligated to pBR 322 and transformed into E. coli. Through the negative selection and colony hybridization, the transformants were obtained. The recombinant plasmids, pNEL 10 and pNES 20 were extracted from these transformants. It was known from Southern hybridization that pNEL 10 contained the 12 Mdal foreign DNA fragment hybridized with nif Q-X probe and pNES 20 included the 5 Mdal foreign DNA fragment hybridized with nif NE and nif YK probe.

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Effect of Electrical Stimulation and Delayed Chilling on the Physicochemical Characteristics of Hanwoo Beef (한우 도체에 대한 전기자극 및 지연냉각이 쇠고기의 이화학적 특성에 미치는 영향)

  • 김대곤;안동현;김수민;성삼경
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.2
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    • pp.207-213
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    • 1998
  • These experiments were carried out to investigate the effects of electrical stimulation(ES) and delayed chilling (DC) on the quality characteristics of Hanwoo beef. The left half carcass was treated with ES(550V, 90sec)within postmortem 30min. The electrical stimulated half carcass was subjected to chilling at 16$^{\circ}C$ for 24hr, and then stored at 2$\pm$2$^{\circ}C$ for 15days (ESDC). The right half carcass was stored at 2$\pm$2$^{\circ}C$ for 15 days (NES). ESDC showed a rapid pH fall and tended to reach to pH5.54 at postmortem 2 hrs. But, there was no consistent effect of electrical stimulation and delayed chilling on meat color, cooking loss and water holding capacity. Myofibril fragmentation index was higher than that of NES during storage. ESDC showed lower shear force value and strength consistently than NES. SDS-PAGE band patterns of myofibrils showed the rapid breakdown of troponin T and troponin I band in ESDC, compared with NES, and revealed the specific band below myosin light chain-2 pattern in ESDC.

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Passive suppression of helicopter ground resonance instability by means of a strongly nonlinear absorber

  • Bergeot, Baptiste;Bellizzi, Sergio;Cochelin, Bruno
    • Advances in aircraft and spacecraft science
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    • v.3 no.3
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    • pp.271-298
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    • 2016
  • In this paper, we study a problem of passive suppression of helicopter Ground Resonance (GR) using a single degree freedom Nonlinear Energy Sink (NES), GR is a dynamic instability involving the coupling of the blades motion in the rotational plane (i.e. the lag motion) and the helicopter fuselage motion. A reduced linear system reproducing GR instability is used. It is obtained using successively Coleman transformation and binormal transformation. The analysis of the steadystate responses of this model is performed when a NES is attached on the helicopter fuselage. The NES involves an essential cubic restoring force and a linear damping force. The analysis is achieved applying complexification-averaging method. The resulting slow-flow model is finally analyzed using multiple scale approach. Four steady-state responses corresponding to complete suppression, partial suppression through strongly modulated response, partial suppression through periodic response and no suppression of the GR are highlighted. An algorithm based on simple criterions is developed to predict these steady-state response regimes. Numerical simulations of the complete system confirm this analysis of the slow-flow dynamics. A parametric analysis of the influence of the NES damping coefficient and the rotor speed on the response regime is finally proposed.

Relationship Between Neurologic Soft Signs and Some Clinical Variables in Patients with Schizophrenia (정신분열증환자의 연성 신경학적 증상과 임상변인과의 관련성)

  • Chae, Jeong-Ho;Habm, Woong;Lee, Chung-Kyoon
    • Korean Journal of Biological Psychiatry
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    • v.2 no.1
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    • pp.115-122
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    • 1995
  • This study was performed to know the relationship between neurologic soft signs (NSS) and clinical variables such as psychopathology. history of illness, and premorbid social adjustment in patients with schizophrenia. The authors evaluated NSS in 31 patients with schizophrenia using the structured tool for measuring neurologic abnormalities, Neurological Evaluation Scale- Korean Version(NES-K). Relationships between NSS and clinical variables such as duration of illness, intensity of precipitating stressors, duration of outpatient treatment, schooling, peer relationship, total duration of unemployment, total days of psychiatric admission, age, total days of being medicated, age at the first psychiatric admission, frequency of admissions, content of treatment, social adjustment, and severity of symptoms were analyzed. Differences between paranoid and non-paranoid schizophrenics were examined. In addition, Differences between patients with schizophrenia who have predominant positive symptoms and who have predominant negative symptoms were examined too. Total scores of NES-K were correlated with lower schooling (${\gamma}$=0.44, p<0.01). Scores of motor coordination subcategory were correlated with poor peer relationship(${\gamma}$=0.67, p<0.001). Other clinical variables were not correlated with any scores of NES-K. Paranoid and non-paranoid schizophrenics were not different in scores of NES-K. Also positive and negative schizophrenics were not different in scores of NES-K. Most clinical variables except schooling and peer relationship were not related with NSS. This results indicated that the meaning of these signs was not fully be understood. Introduction of the new classification concepts such as deficit or non-deficit syndrome will be helpful to elucidate the meaning of NSS in patients with schizophrenia.

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A Study on the Toxic Gases and Smoke Hazard of PASCON Trough (파스콘 트로프의 연기유독성에 관한 연구)

  • Lee, Chang-Woo;Hyun, Seong-Ho;Choi, Don-Mook
    • Fire Science and Engineering
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    • v.20 no.2 s.62
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    • pp.1-7
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    • 2006
  • The aim of the research is to estimate the effect of smoke and combustion gases on humane body indirectly through measuring the toxicity of those. For this purpose, the toxic index of smoke and combustion gases was investigated by smoke hazard test and analysis of smoke which were conducted by KS F 2271 and NES 713 method respectively. It i s proved by KS F 2271 method that PASCON trough is suitable to the testing standard of interior material and construction of building. In addition, it is identified by NES 713 method that combustion gases occurring in PASCON product were only carbon dioxide and carbon monoxide, and the smoke index of those was 0.944. This value means that the hazard effect of smoke gases on humane ! body can possibly happens when exposed to the smoke gases for more than 30 min. In aspect of the domestic situation that have not regulated the hazard estimation and the emissions of smoke when the flame retarding ability of the products have been requested, the toxic indexes of PASCON products are comparatively low.

Design of 3D compression upper wear based on skin deformation during arm abduction (팔 외전 시 몸통의 피부 변화량 분석과 이를 활용한 3D 컴프레션 상의 설계)

  • Kim, Nam Yim;Wu, Yan Jun;Hong, Kyunghi
    • Korean Journal of Human Ecology
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    • v.24 no.5
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    • pp.687-700
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    • 2015
  • Lines of non-extension (LoNEs) on torso surface during arm abduction were investigated to provide appropriate location for inserting less-extensible yarns which can be used as seams for design and or clothing pressure variation. As experimental methods, reference points about 3 cm apart were marked on the skin and scanned at 30, $90^{\circ}$ and $135^{\circ}$ arm abduction. Skin deformation was measured by connecting reference points in horizontal, vertical and various angles of diagonal directions. Observation of skin deformation was made within the separated sections of the torso as well as integrated ones to cover the various occasions of design application. LoNEs of front and back torso were provided as mapping lines. Actual compression wear of three types was constructed with different pattern reduction rate at each separated section using LoNEs as boundary cutting lines. Clothing pressure and subjective evaluations of those three compression wear were evaluated by six subjects. LoNEs found in this study were useful as seam lines to differentiate clothing pressure at each part of the body, providing positive wear sensation. It is also expected that LoNEs can be paths for less strechable conductive yarns of IT-integrated upper garments.

Experimental evaluation of the performance of self-compacting concrete contains nano clay and nano egg shell

  • Hilal, Nahla N.;Hadzima-Nyarko, Marijana
    • Advances in concrete construction
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    • v.13 no.5
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    • pp.349-360
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    • 2022
  • The rising prices of landfills and the lack of cement production are motivating researchers to be more interested in using wastes to produce concrete mixtures materials. The use of waste materials such as eggshell and matakoline waste not only reduces landfill costs and space, but also reduces the cost of cement production for the concrete mixture. However, recycling waste materials has become critical in order to effectively manage environmental sustainability. The purpose of this paper is to investigate the appropriate properties of self-compacting concrete (SCC) by incorporating waste materials such as crushed ceramics as coarse aggregate and nano egg shell (NES) and nanoclay (NC) as cement replacements. Fresh properties of SCC, such as segregation, flow time and diameter, V-funnel, H2/H1 ratio, and fresh unit weight of concrete mixtures, as well as hardened properties, such as 7, 14, and 28 days compressive strength and 28 and 90 days flexural strength, were measured for this purpose. The presence of NC in the SCC mixture enhanced the compressive strength of the concrete when 5% of NES was added or in the case without the addition of NES compared to the control mixture. The flexural strength enhanced with the incorporation of NC in the SCC increased the flexural strength of the concrete compared to the control mixture, but the incorporation of 5% of NES decreased the flexural strength compared to the mixtures with NC. These results prove the possibility of using crushed ceramics as the coarse aggregate, and NES and NC as substitutes for 5, 7, and 10% of the cement in SCC, because the properties of such SCC in hardened and fresh states are satisfactory.

Hazard Assessment of Combustion Gases from Interior Materials (주요 건축 내장재의 연소가스 유해성 평가)

  • Seo, Hyun Jeong;Son, Dong Won
    • Fire Science and Engineering
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    • v.29 no.4
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    • pp.49-56
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    • 2015
  • Toxic gases from five types of interior building materials were investigated according to Naval Engineering Standard (NES) 713. The materials were plywood, indoor wall coverings (wood wall plate members and pine wood), reinforced Styrofoam insulation, laminate flooring, and PVC. Specimens were measured using an NES 713 toxicity test apparatus to analyze the hazardous substances in combustion gas from the materials. We used the US Department of Defense standard (MIL-DTL, Military Standard) to calculate the toxicity index of the combustion gas. Emissions of $CO_2$ from all specimens did not exceed the NES 713 limit of 100,000 ppm. The amount of CO gas emissions from reinforced Styrofoam insulation was 6,098 ppm. 25 ppm and 49 ppm of formaldehyde were released from the reinforced Styrofoam insulation and PVC flooring, respectively. These values were less than the limit of 400 ppm. The highest emissions were from $NO_X$ emitted by plywood and were above the limit of 250 ppm. The toxicity index of the specimens were calculated as 5.19 for plywood, 4.13 for PVC flooring, 2.35 for reinforced Styrofoam insulation, 2.34 for laminate flooring, and 1.22 for indoor wall coverings (pine wood). Our research helps us to understand the properties of these five interior materials by analyzing the combustion gas and explaining the toxicity of constituents and the toxicity index. Also, it would be useful for giving fundamentals to guide the safe use of interior materials for applications.

Changes of Sugar Content by Different Storage Durations in Sweet Corn and Super Sweet Corn (단옥수수와 초당옥수수의 저장기간에 따른 당함량의 변화)

  • 박승의;차선우;손영희;손영구
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.1
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    • pp.79-84
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    • 1994
  • Sweet corn hybrid GCB 70 and super sweet corn hybrid NES were cultivated at vinyl tunnel in 1991 to determine the physiologically optimum harvest time for higher eating quality and sugar contnent of kernel by eat parts after silking and stored in refrigerator of 4$^{\circ}C$ and in the air. The development of ear increased continuously from silking to 30th day after silking, and sugar content was the hightest on 15th day after silking. The physiologically optimum harvest time for GCB 70 and NES was about 15~20th day and 20~25th day after silking, respectively. The sugar content of kernel by ear parts for two hybrids were high in order of basal, middle and top parts of the ear and showed significant differences among the parts of the ear. Changes of total sugar content during the storing period at 4$^{\circ}C$ for NES was almost the same by 9th day, but for GCB 70 rapidly decreased from 3rd day. The content of sucrose, glucose and fructose in super sweet corn NES were much higher than those of sweet corn GCB 70, but the maltose content of NES was lower than that of GCB 70.

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