• The Plant Pathology Journal
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• v.36 no.1
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• pp.106-110
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• 2020

Citrus mosaic sadwavirus (CiMV) is a closely related virus with the Satsuma dwarf virus (SDV) along with Navel orange infectious mottling virus (NIMV), Natsudaidai dwarf virus (NDV), and Hyugagatsu virus (HV). The present study found that the typical symptoms of CiMV-infected citrus fruits include the appearance of dark blue speckles or ringspots on fruit rinds and the browning of oil glands in the spots as rind coloring began. As rind coloring progressed, the spots gradually faded, whereas the browning of the oil glands worsened to the point that the tissues surrounding the oil glands became necrotic. In very early satsuma mandarins (Citrus unshiu 'Miyamoto Wase') and 'Setoka' cultivar (C. hybrid 'Setoka') of late-maturity citrus, the symptomatic fruits were eventually dropped. And in early satsuma mandarin (C. unshiu 'Miyakawa Wase'), the peel hardness of the virus-infected fruit (1,618.3 ± 305.5, g-force) was more than twice as hard as that of the healthy fruit (636.5 ± 39.1, g-force). The ratio of flesh weight to total fruit weight was higher for the healthy fruit (77.3 ± 1.7%) than for the infected fruit (70.7 ± 0.6) and peel puffing was more severe in the infected fruit (2.9 ± 0.4 mm) than in the healthy fruit (0.9 ± 0.2 mm). The soluble solids content in infected citrus fruits was less values than the healthy fruit by 0.5-1.5 °Brix. These findings reveal that CiMV infection on citrus trees reduces the fruit quality of citrus.

• Korean Journal of Veterinary Service
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• v.24 no.1
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• pp.21-29
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• 2001

To compare of serum antibody titers using ELISA and HI, serum samples were collected from 100 breeders and their progeny 550 broilers. The breeders and broilers were vaccinated with infectious bronchitis(IB)- and Newcastle disease(ND)-viruses according to general vaccination program. The antibodies in serum samples against IB and ND viruses were detected by enzyme-linked immunosorbent assay(ELISA) using commercial ELISA kit and hemagglutination inhibition(HI) test. Geometric mean titer(GMT) of ELISA and In titers were monitored from 1-day-old to 35-day-old broilers and compared to those of breeder chickens. The antibody titers of breeders vaccinated with ]B virus showed 47,800, ELISA and 7.2, HI, respectively. Progeny chicks, 1-day-old, vaccinated with IBV showed high antibody titers than those of breed chickens. Those chicks were maintained protective antibody levels until 11-day-old. From 14-day-old, the antibody level decreased below protective levels. In ND, breeders serum antibody titers ELISA and Eiu were 30,200 GMT and 8.7 HI titer, respectively. On 1-day-old chicks, antibody levels was decreased to half in ELISA(16,270) compared with those of breeders, but In titers was 7.4. Progeny broilers, protective antibody level was maintained until 14- day-old by ELISA, but at 11-day-old by HI titers. After then, ND antibody titer was continuously decreased underdefense level. These result indicated that the ELISA method be more sensitive than HI titration to detect serum antibody level for IBV and NDV.

• Korean Journal of Veterinary Research
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• v.46 no.3
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• pp.185-196
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• 2006

Newcastle disease (ND) is a highly contagious disease of poultry that can cause severe economic losses throughout the world. Vaccination has been used for a long time and proved as one of the most effective method to reduce the economic loss due to ND virus infection, The measurement of antibody titer such as hamagglutination-inhibition (Hl) test with sera has been used as a useful method to evaluate the immunity leve of host. However, Hl test is gradually being replaced by the enzyme linked-immunosorbent assay (ELISA), To evaluate the efficacy of ELISA in the chickens vaccinated with different procedure, present study has been performed. After SPF chicks and commercial broilers were vaccinated with different kinds of live vaccines such as V4, VG/GA and/or Bl at various time, the antibody level has been measured using both HI test and ELISA. Challenge test with velogenic viscerotropic NDV was also performed to measure the protective level of antibody. In the SPF chickens, the mean ELISA titer after vaccination and survival rate after challenge was increased and correlated with days post inoculation. More than 80% of chickens with higher than 1,000 ELISA titer after vaccination were survived after challenge with velogenic ND virus and had good correlation between survival rate and antibody titier. In commercial broiler chickens, most of them at market age had low level of ELISA titer regardless of the number of vaccination, and had a low correlation between survival rate and ELISA titer. However, the ELISA titer of remaining birds after challenge was increased. This result indicated that ELISA titer had good response against velogenic NOV infection compared to Hl titer.

• Proceedings of the Korea Water Resources Association Conference
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• 2018.05a
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• pp.23-23
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• 2018

본 연구에서는 다중분위회귀분석모형(Multiple Quantile Regression Model, MQRM)과 MODIS(MODerate resolution Imaging Spectroradiometer) LST (Land Surface Temperature) 자료를 이용하여 전국 공간토양수분을 산정하였다. 공간토양수분을 산정하기 위한 과정은 크게 두가지로 구분된다. 첫 번째로 기존의 MODIS LST 자료를 조건부 합성 보정기법을 적용하여 실측 LST 자료와 비교하여 위성 LST 자료가 갖고 있는 오차를 보정하였다. 그 결과, 조건부 합성 보정기법을 적용하기전 전국 71개 지상관측지점에서 관측한 실측 LST와 MODIS LST의 $R^2$는 전체 평균 0.70으로 어는정도 유의성 있는 상관관계를 나타냈으나 조건부 합성 보정기법을 적용한 후 실측 LST와 MODIS LST의 $R^2$는 전체 평균 0.92로 상당히 크게 향상됨을 알 수 있었다. 두 번째로 보정된 MODIS LST를 이용하여 다중분위회귀분석 모형을 개발하고 토양수분을 예측하는 단계로 입력자료로 위성영상 자료와 관측자료를 융합하여 사용하였다. 위성영상 자료로는 보정된 MODIS LST와 MODIS NDV를 구축하였고 일단위 강수량 및 일조시간의 기상자료는 기상청으로부터 전국 71개 지점에 대해 구축하여 IDW 공간보간기법을 이용한 공간자료로 구축하였다. 토양수분 결과를 비교하기 위한 관측 토양수분은 자동농업기상관측(Automated Agriculture Observing System, AAOS)지점에서 2013년 1월부터 2015년 12월까지의 실측 일단위 토양수분 자료를 구축하여 사용하였다. 다중분위회귀분석 모형은 LST 인자를 중심으로 각각의 분위(0.05, 0.25, 0.5, 0.75, 0.95)에 해당되는 값의 회귀식을 NDVI, 강수 입력자료를 독립인자로서 조합하여 계절 및 토성에 따른 총 80개의 회귀식을 산정하였다. 관측 토양수분과 모의 토양수분을 비교한 결과 $R^2$가 0.70 (철원), 0.90 (춘천), 0.85 (수원), 0.65 (서산), 0.78 (청주), 0.82 (전주), 0.62 (순천), 0.63 (진주), 0.78 (보성)로 높은 상관성을 보였다. 본 연구에서는 다중분위회귀 모형의 성능을 검증하기 위해 기존의 다중선형회귀모형의 결과와 비교하여 크게 개선됨을 나타냈다.

• Proceedings of the Korea Water Resources Association Conference
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• 2017.05a
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• pp.103-104
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• 2017

본 연구에서는 다중회귀분석모형(MLRM)과 MODIS (MODerate resolution Imaging Spectroradiometer) LST (Land Surface Temperature) 자료를 이용하여 전국 공간토양수분을 산정하였다. 공간토양수분을 산정하기 위한 과정은 크게 두가지로 구분된다. 첫 번째로 기존의 MODIS LST 자료를 조건부 합성 보정기법을 적용하여 실측 LST 자료와 비교하여 위성 LST 자료가 갖고 있는 오차를 보정하였다. 그 결과, 조건부 합성 보정기법을 적용하기전 전국 71개 지상 관측지점에서 관측한 실측 LST와 MODIS LST의 R2는 전체 평균 0.70으로 어는정도 유의성 있는 상관관계를 나타냈으나 조건부 합성 보정기법을 적용한 후 실측 LST와 MODIS LST의 R2는 전체 평균 0.92로 상당히 크게 향상됨을 알 수 있었다. 두 번째로 보정된 MODIS LST를 이용하여 다중회귀분석 모형을 개발하고 토양수분을 예측하는 단계로 입력자료로 위성영상 자료와 관측자료를 융합하여 사용하였다. 위성영상 자료로는 보정된 MODIS LST와 MODIS NDV를 구축하였고 일단위 강수량 및 일조시간의 기상자료는 기상청으로부터 전국 68개 지점에 대해 구축하여 IDW 공간보간기법을 이용한 공간자료로 구축하였다. 토양수분 결과를 비교하기 위한 관측 토양수분은 자동농업기상관측(Automated Agriculture Observing System, AAOS)지점에서 2013년 1월부터 2015년 12월까지의 실측 일단위 토양수분 자료를 구축하여 사용하였다. 다중회귀분석 모형은 각각의 입력자료를 독립인자로서 조합하여 12개의 시나리오를 만들었다. 시공간적 경향을 고려하기 위하여 계절별, 토양 토성(soil texture)를 구분하여 회귀분석을 실시하였다. 관측 토양수분과 모의 토양수분을 비교한 결과 $R^2$가 0.80 (철원), 0.90 (춘천), 0.80 (수원), 0.63 (서산), 0.77 (청주), 0.82 (전주), 0.52 (순천), 0.63 (진주), 0.99 (보성)로 높은 상관성을 보였다. 본 연구에서는 토양수분을 예측하기 위한 인자 중 가장 민간함 LST를 보정하지 않는 토양수분 예측 방법은 상당한 오차를 포함하게 되어 실측 토양수분 결과와 크게 차이가 나타남을 보여주었다.

• Journal of Veterinary Science
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• v.23 no.3
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• pp.29.1-29.6
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• 2022

In September 2017, an outbreak with high mortality, which showed the typical signs of ND, occurred among a flock of more than 2000 Eurasian collared doves in Konarak, southeast of Iran. A confirmed pigeon paramyxovirus type 1 strain was isolated from the brain tissues of the dead doves. The isolate, which was called Pigeon/Iran/Konarak/Barin/2017, was classified as a highly velogenic NDV. Complete genome sequencing and phylogenetic analysis showed that the isolate belonged to subgenotype XXI.2, which has never been reported from Iran before. The isolate had the highest homology (96.15%) with early 2010s Italian isolates. Further studies will be required to understand the diversity better.

• Korean Journal of Poultry Science
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• v.35 no.4
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• pp.351-359
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• 2009

This study was carried out to investigate dietary effects of extracts of Skullcap (Scutellaria baicalensis) (SCE) grown in Korea on growth performance, immune and physiological responses in broiler chickens. Total of seven-hundred fifty 1-d-old Ross male broiler chicks were divided into five groups and fed control diets (antibiotics medicated or non-medicated commercial diets) or each experimental diet (non-medicated diets containing 0.1, 0.3 or 0.5% SCE) for 5 weeks. The body weight gain and feed conversion rate in the groups fed diets containing 0.1% or 0.3% SCE were significantly improved as compared with those of non-medicated control group (P<0.05). The levels of total cholesterol and HDL-cholesterol of blood were not influenced by feeding the SCE. The average antibody titers against NDV and IBV in the groups fed diets containing SCE were significantly increased compare to those of the control groups (P<0.05). The number of coli form bacteria was significantly reduced by feeding 0.3% or 0.5% SCE as compared to that of non-medication control (P<0.05). The results demonstrated that the SCE used in this study modulated humoral immunity and the profiles of cecal microflora and thus can be used as a potential alternative substance to replace antibiotics for feeding broiler chicks.

• Korean Journal of Poultry Science
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• v.15 no.3
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• pp.193-198
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• 1988

In order to examine the efficacy of concurrent vaccination with live and killed Newcastle disease(ND) vaccines two types of each live($B_1$ and LaSota) and killed(gel and oil) vaccines of all commercial origin were administered either alone or simultaneously to day-old broiler chicks having maternal antibody. Live vaccines were given by conjuntival instillation in volumes of 25${\mu}\ell$ containing $10^{6.0}$ to $10^{6.3}$ median embroy infective dose(EH)) while killed vaccines were given in 0.3$m\ell$ volumes subcutaneously at the back of the neck Hemagglutination inhibition(HI) antibodies were determined at weekly intervals until 8 weeks of age and protection rate was determined at 4 and 8 week of age by challenge inoculation with virulent ND virus(NDV). During the 8 weeks experimental period concurrent administration of live and oil vaccine produced the highest level of HI antibody and the most satisfactory protection, whereas concurrent rent vaccination with live and gel vaccine induced poor immune responses. There was no noticeable difference in the efficacy between the live vaccines, Bl and LaSota when simultaneously administered with oil vaccine. Except for oil vaccine, single administration of either live or killed vaccine at day-old produced less than 50% protection at 4 and 8 weeks postvaccination(PV). Oil vaucine alone induced 80% and 70% protection at 4 and 8 week PV, respectively. Concurrent vaccination caused on visible side reaction like respiratory symptoms and did not negatively influence the growth rate of birds until the end of experiment.

• Korean Journal of Poultry Science
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• v.41 no.3
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• pp.173-179
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• 2014

While use of mass rearing systems improved poultry production, chances of exposing to contagious diseases have been increased, making flocks more vulnerable to diseases. Diseases of interest which affects egg production adversely include Low pathogenic avian influenza (LPAI), Infectious bronchitis (IB), Avian meta-pneumoviral infection (aMPV) and Egg drop syndrome'76 (EDS'76). This report collected and analyzed 5,385 serum samples, which were collected from 1,330 different chicken flock, provided by Chungbuk National University, Avian Disease Laboratory at 2009. Serums were analyzed based on rearing stages; 0~1.3weeks (wks) (maternal antibody period), >1.3~3 wks (starting period), >3~10 wks (growing period), >10~22 wks (developing period), >22~40 wks (peak laying period), >40~60 wks (late laying period) and over 60 wks (post-molting period). Results showed the 99.7% of the tested flocks were immunized against ND and73.8%, 97.1%, 78,2% and 78% of the flocks were immunized against other 4 agents (LPAI, IB, EDS'76, aMPV). Maternal antibody was transferred to enough quantity for NDV. Generally, antibody titers which were developed at 22 weeks were stabilized permanently for life. In case of IB and aMPV, infection titer emerged as early as 10 weeks and the titer was increased from 99.4% to 100% for life. EDS76 showed increase in titers, reflecting decreased frequency of vaccination programs. Overall, this study displayed general trends of major viral disease in layers, but considering the trend of development of preventive measures and evolution of pathogens, conducting serological surveys on a regular basis is important.

• Spatial Information Research
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• v.21 no.2
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• pp.55-71
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• 2013

This study assessed the thermal comfort of new towns in the Seoul Metropolitan Area (Ilsan, Bundang, Dongtan1) using PET (Physiologically Equivalent Temperature) which refers to real human heat stress. The relationship between PET and urban spatial elements was also analyzed using multiple regression analysis. The study results show that the thermal comfort of Dongtan 1, which is considering a reduction of the urban heat island effect in the planning phase, is higher than other cities. In addition, through regression results, the impervious ratio, floor area ratio, commercial area ratio, and residential area ratio were found to be major factors increasing PET. Moreover, the river area ratio and NDVI were found to be major factors decreasing PET. This study has scientific significance as research that focuses on the assessment of thermal comfort scientifically and definitely, by estimating PET for an entire urban area using GIS analysis that included remote sense analysis and the wind field model. The results of this study can be used in preparing more effective urban plans for the promotion of citizen thermal comfort.