Browse > Article

Efficacy of ELISA for measurement of protective newcastle disease antibody level in broilers  

Kim, Jong-Nyeo (Research Institute of Veterinary Medicine/College of Veterinary Medicine, Chungbuk National University)
Heo, won (Daessung Microbiological Labs. Co. Ltd.)
Mo, In-Pil (Research Institute of Veterinary Medicine/College of Veterinary Medicine, Chungbuk National University)
Publication Information
Korean Journal of Veterinary Research / v.46, no.3, 2006 , pp. 185-196 More about this Journal
Abstract
Newcastle disease (ND) is a highly contagious disease of poultry that can cause severe economic losses throughout the world. Vaccination has been used for a long time and proved as one of the most effective method to reduce the economic loss due to ND virus infection, The measurement of antibody titer such as hamagglutination-inhibition (Hl) test with sera has been used as a useful method to evaluate the immunity leve of host. However, Hl test is gradually being replaced by the enzyme linked-immunosorbent assay (ELISA), To evaluate the efficacy of ELISA in the chickens vaccinated with different procedure, present study has been performed. After SPF chicks and commercial broilers were vaccinated with different kinds of live vaccines such as V4, VG/GA and/or Bl at various time, the antibody level has been measured using both HI test and ELISA. Challenge test with velogenic viscerotropic NDV was also performed to measure the protective level of antibody. In the SPF chickens, the mean ELISA titer after vaccination and survival rate after challenge was increased and correlated with days post inoculation. More than 80% of chickens with higher than 1,000 ELISA titer after vaccination were survived after challenge with velogenic ND virus and had good correlation between survival rate and antibody titier. In commercial broiler chickens, most of them at market age had low level of ELISA titer regardless of the number of vaccination, and had a low correlation between survival rate and ELISA titer. However, the ELISA titer of remaining birds after challenge was increased. This result indicated that ELISA titer had good response against velogenic NOV infection compared to Hl titer.
Keywords
Newcastle disease; Hl; ELISA; challenge infection;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Korani T, Odagiri Y, Nakamura J, Horlughi T. Pathological changes of tracheal mucosa in chickens infected with lentogenic NDV. Avian Dis. 1987, 31, 491-497   DOI   ScienceOn
2 Parry SH, Aitken ID. Local immunity in the respiratory tract of the chicken, II The secretory immune response to Newcastle disease virus and the role of IgA. Vet. Microbiol. 1977, 2, 143-165   DOI   ScienceOn
3 Reed LJ, Muench H. A simple method for estimating fifty percent endpoints. Am. J. Hyg. 1938, 27, 493-497
4 Reynlods DL, Maraqa AD. Protective immunity against Newcastle disease virus: The role of antibodies specific to Newcastle disease virus polypeptides. Avian Dis. 2000, 44, 138-144   DOI   ScienceOn
5 Steward M, Vipond IB, Millar NS, Emmerson PT. RNA editing in Newcastle disease virus. J. Gen. Virol. 1993, 76, 2519-2527
6 Alexander DJ. Newcastle disease in countries of the European Union. Avian Pathol. 1995, 24, 3-10   DOI   ScienceOn
7 Reynolds DL, Maraqa AD. Protective immunity against Newcastle disease virus: The role of cell-mediated immunity. Avian Dis. 2000, 44, 145-154   DOI   ScienceOn
8 Samberg Y, Hornstein K, Cuperstein E, Rivka G. Spray vaccination of chickens with an experimental vaccine against Newcastle disease. Avian Pathol. 1977, 6, 251-258   DOI
9 Meulemans G, Gonze M, Carlier MC, Petit P, Burny A, Le Long L. Protective effect of HN and F glycoprotein-specific monoclonal antibodies on experimental Newcastle disease. Avian Pathol. 1986, 15, 761-768   DOI
10 Alexander DJ. Newcastle disease and other avian paramyxoviridae infections. In Calnek BW, with John Barnes H, Beard CW, McDougald LR, Saif YM. (eds.), Disease of poultry. 1997, 10th ed, Iowa state University Press. Ames. IA. pp. 541-569
11 Giambrone JJ. Laboratory evaluation of Newcastle disease vaccination programs for broiler chickens. Avian Dis. 1985, 29, 479-487   DOI   ScienceOn
12 Fedova D, Novotny J, Kubinova I. Serological diagnosis of parainfluenza virus infections: Verification of the sensitivity and specificity of the heamagglutination-inhibition (HI), complement-fixation (CF), immunofluorescence (IFA)test and enzyme immunoassay (ELISA). Acta virol. 1992, 36, 304-312
13 Alexader DJ, Collins MS. The structural polypeptides of avian paramyxovirises. Arch. Virol. 1981, 67, 309-323   DOI
14 Stone-Hulslander J, Morrison TG. Detection of an interaction between the HN and F proteins in Newcastle disease virus-infected cells. J. Virol. 1997, 71, 6287-6295
15 Allan WH, Lancaste JE, Toth B. Newcastle disease vaccines-their production and use. Animal Production and Health Series No. 10. 1978 FAO, Rome, Italy
16 Russell PH. Newcastle disease virus: Virus replication in the Harderian gland stimulates lacrimal 19A; the yolk sac provides early lacrimal IgG Vet. Immunol. Immunopathol. 1993, 37, 151-163   DOI   ScienceOn
17 송창선, 이윤정, 한명국, 성환우, 강경수, 이중복, 김재학 등. 최근 야외농장에서 실시하고 있는 뉴캣슬병 생독백신 접종효능에 대한 평가. 대한수의학회지. 2000, 40(3), 563-573
18 Marino OC, Hanson RP. Cellular and humoral response of in ovo-bursectomized chickens to experimental challenge with velogenic Newcastle disease virus. Avian Dis. 1987, 31, 293-301   DOI   ScienceOn
19 Marquardt WW, Snyder DB, Savage PK, Kadavil SK, Uancey FS. Atibody response to Newcastle disease virus given by two different routes as measured by ELISA and Hemagglutination-Inhibition test and associated tracheal immunity. Avian Dis. 1985, 29, 71-79   DOI   ScienceOn
20 Pipkin PA, Afzal MA, Heath AB, Linor PD. Assay of humoral immunity to mumps virus. J. Vrol. Methods. 1999, 79, 219-225   DOI   ScienceOn
21 Holmes HC. Virus neutralizing antibody in sera and secretion of the upper and lower respiratory tract of the chicken inoculatecd with live and inactivated Newcastle disease. J. Comp. Pathol. 1979, 89, 21-29   DOI   ScienceOn
22 Iorio R, Bratt M. Neutralization of Newcastle disease virus by monoclonal antibodies to the hemagglutinin-neuraminidase glycoprotein: requirement for antibodies to four sites $f_0$ complete neutralization. J. Virol. 1984, 51, 445-451
23 Kant A, Koch G, Van Roozelaar DJ, Balk F, Ter Huurne A. Differentiation of virulent and non-virulent strains of newcastle disease virus within 24 hours by polymerase chain reaction. Avian Pathol. 1997, 26, 837-849   DOI   ScienceOn
24 Timms L, Alexander DJ. Cell-mediated immune response of chickens to Newcastle disease vaccines. Avian Pathol. 1977, 6, 51-59   DOI
25 Al-Garib SO, Gielkens ALJ, Gruys E, Hartog L, Koch G. Immunoglobulin class distribution of systemic and mucosal antibody response to Newcastle diasease in chickens. Avian Dis. 2003, 47, 32-40   DOI   ScienceOn
26 Daniele RP. Immunoglobulin secretion in the airway. Ann. Rev. Physiol. 1990, 52, 177-195   DOI   ScienceOn
27 Holmes HC. Resistance of the respiratory tract of the chicken to Newcastle disease virus infection flowing vaccination: The effect of passively acquired antibody on its development. J. Comp. Pathol. 1979, 89, 11-19   DOI   ScienceOn
28 Miers LA, Bankowski RA, Zee YC. Optimizing the enzyme-linked immunosorbent assay for evaluating immunity of chickens to Newcastle disease. Avian Dis. 1983, 27, 1112-1125   DOI   ScienceOn
29 Adair BM, McNulty, MS, Todd D, Connor TJ, Burns K. Quantitative estimation of Newcastle disease virus antibody levels in chickens and turkeys by ELISA. Avian Pathol. 1989, 18, 175-192   DOI
30 Alexander DJ. Newcastle disease in ostriches (Struthio camelus)-a review. Avian Pathol. 2000, 29, 95-100   DOI
31 Iorio RM, Borgman JB, Glickman RL, Bratt M. Genetic variation within a neutralizing domain on the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus. J. Gen. Virol. 1986, 67, 1393-1403   DOI   ScienceOn
32 Wilson RA, Charles PJ, Betsy F, Robert JE. An enzyme-linked immunosorbent assay that measures protective antibody levels to Newcastle disease virus in chickens. Avian Dis. 1984, 28, 1079-1085   DOI   ScienceOn
33 Jayawardane GW, Spradbrow PB. Mucosal immunity in chickens vaccinated with the V4 strain of Newcastle disease virus. Vet Microbiol. 1995, 46, 69-77   DOI   ScienceOn
34 Russell PH, Dwivedi PN, Davison TF. The effect of cyclosporin A and cyclophosphamide on the populations of B and T cells and virus in the Harderian of chickens vaccinated with the Hitcher B1 strain of Newcastle disease virus. Vet. Immunol. Immunopathol. 1997, 60, 171-185   DOI   ScienceOn
35 De Leevw O, Peeters B. Complete nucleotide sequence of Newcastle disease virus: Evidence for the existence of a new genus within the subfamily Paramyxovirinae. J. Gen. Virol. 1999, 80, 131-136   DOI