• Membrane and Water Treatment
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• v.4 no.1
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• pp.1-9
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• 2013

The process of electromembrane transformation of L-lysine monohydrochlorides into their zwitterionic form in four- and two-chamber electrodialysis apparatus was investigated. The process of transformation at various concentrations of lysine monohydrochloride (0.1-0.6 mol.L-1) was studied and it was established that at the optimum density of current optimal concentrations of lysine hydrochloride during electrodyalisis was in the range of 0.2-0.4 mol.L-1. It was determined that the process of total transformation was accomplished when pH of the lysine solution achieved 10. Changes of concentrations of $Cl^-$ ions and lysine diffused into the neighboring chamber were determined depending on the time. The method developed by us allows adjusting the removal coefficient of $Cl^-$ ions during transformation to a maximal value, the losses of lysine diffused into the next chamber after its return to the technological cycle being less than 1.0 %. The specific energy consumption during the process of transformation in two- and four-chamber electrodialyzers was 0.19 and 0.205 A.h.kg-1 and the current efficiency was 75.9 and 73.1 %, correspondingly. Study of the process of electromembrane transformation allowed obtaining zwitterionic form of L-lysine from L-lysine monohydrochloride with minimal reagent and energy consumption.

• Journal of Information Processing Systems
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• v.18 no.2
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• pp.188-196
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• 2022

Recently, neural architecture search (NAS) has received increasing attention as it can replace human experts in designing the architecture of neural networks for different tasks and has achieved remarkable results in many challenging tasks. In this study, a path-based computation neural architecture encoder (PCE) was proposed. Our PCE first encodes the computation of information on each path in a neural network, and then aggregates the encodings on all paths together through an attention mechanism, simulating the process of information computation along paths in a neural network and encoding the computation on the neural network instead of the structure of the graph, which is more consistent with the computational properties of neural networks. We performed an extensive comparison with eight encoding methods on two commonly used NAS search spaces (NAS-Bench-101 and NAS-Bench-201), which included a comparison of the predictive capabilities of performance predictors and search capabilities based on two search strategies (reinforcement learning-based and Bayesian optimization-based) when equipped with different encoders. Experimental evaluation shows that PCE is an efficient encoding method that effectively ranks and predicts neural architecture performance, thereby improving the search efficiency of neural architectures.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.68-68
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• 2022

The pepper mild mottle virus (PMMoV), belonging to the tobamovirus genus, is currently one of the most destructive pathogens in pepper production. Tobamoviruses have been classified in terms of increased pathogenicity as pathotypes P₀, P₁, P_1,2, P_1,2,3 and P_1,2,3,4, based on their ability to infect systemically Capsicum L⁰ , L¹ , L² , L³ and L⁴ resistant plants, respectively. Two hundred eighty pepper germplasms and 5 reference accessions known as resistant L alleles, were analyzed to select the resistance cultivars against PMMoV- P_1,2,3 (CV130614-2) using bioassay and genetic markers. The susceptible accessions showed systemic symptom when inoculated with PMMoV- P_1,2,3. However, accessions including IT223737, were resistant as they developed necrotic local lesions only on inoculated leaves, whereas no symptoms were observed on the upper leaves. Moreover, RT-PCR results for detecting the presence of virus were also negative. Thus, those accessions will be used as a novel source to facilitate introduction the resistant gene into commercial cultivars of pepper.

• Korean Journal of Plant Resources
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• v.32 no.6
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• pp.730-734
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• 2019

Cryopreservation is one of the ideal and suitable methods for long-term storage of plant germplasm. The plant contaminated with diseases and pathogens are decreased the multiplication rate, survival rate and high quality of plants after cryopreservation. The aim of this work was to improve a micropropagation method for lily in Korea, which is indigenous plant. In the last process of rinsing scales after surface-sterilization, we tried to control the diseases and pathogens lived within the tissue by rinsing in 0.03% sodium hypochlorite (NaClO) instead of sterile distilled water. Bulb scales of Lilium were cultured in vitro on MS medium supplemented with Plant Preservative Mixture (PPM). The results showed that L. tsingtauense accessions were observed ranged from 53.9 to 100% with a mean value of 76.8% and L. hansonii accessions were checked from 84.5 to 85.5% with a mean of 85% survival rate. The newly small bulb formed from bulb-scales was transferred to MS medium. We checked the presence of microorganisms and survival rate after 3 weeks in culture after examination of bacterial incidences. The results indicated that the non-contamination rate were shown ranged from 75.0 to 94.1% with mean value of 83.2% in L. tsingtauense species, and that L. hansonii were observed 85.1 to 91.7% with mean value of 88.4%. This study will provide a valuable basis for establishment of effective axenic cultures for in vitro micropropagation of Korean native lily species.

• Convergence Security Journal
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• v.13 no.3
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• pp.85-90
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• 2013

To provide high-quality services, national public institutions and companies have provided information and materials over the internet network. However, a risk of malware infection between transmission and reception of data leads to exposure to various security threats. For this reason, national institutions have proceeded with projects for network separation since 2008, and data linkage has been made using network connection storage through network separation technologies, along with physical network separation. However, the network connection storage has caused waste of resources and problems with data management due to the presence of the same data in internal network storage and external network storage. In this regard, this study proposes a method to connect internal and external network data using NAS storage as a way to overcome the limitations of physical network separation, and attempts to verify the priority of items for the optimization of network separation by means of AHP techniques.

• Journal of Biosystems Engineering
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• v.41 no.3
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• pp.208-220
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• 2016

Purpose: Fusel oil is a potent volatile aroma compound found in many alcoholic beverages. At low concentrations, it makes an essential contribution to the flavor and aroma of fermented alcoholic beverages, while at high concentrations, it induced an off-flavor and is thought to cause undesirable side effects. In this work, we introduce Fourier transform near-infrared (FT-NIR) spectroscopy as a rapid and nondestructive technique for the quantitative determination of fusel oil in the Korean alcoholic beverage "soju". Methods: FT-NIR transmittance spectra in the 1000-2500 nm region were collected for 120 soju samples with fusel oil concentrations ranging from 0 to 1400 ppm. The calibration and validation data sets were designed using data from 75 and 45 samples, respectively. The net analyte signal (NAS) was used as a preprocessing method before the application of the partial least-square regression (PLSR) and principal component regression (PCR) methods for predicting fusel oil concentration. A novel variable selection method was adopted to determine the most informative spectral variables to minimize the effect of nonmodeled interferences. Finally, the efficiency of the developed technique was evaluated with two different validation sets. Results: The results revealed that the NAS-PLSR model with selected variables ($R^2_{\upsilon}=0.95$, RMSEV = 100ppm) did not outperform the NAS-PCR model (($R^2_{\upsilon}=0.97$, RMSEV = 7 8.9ppm). In addition, the NAS-PCR shows a better recovery for validation set 2 and a lower relative error for validation set 3 than the NAS-PLSR model. Conclusion: The experimental results indicate that the proposed technique could be an alternative to conventional methods for the quantitative determination of fusel oil in alcoholic beverages and has the potential for use in in-line process control.

• Journal of The Korean Society of Agricultural Engineers
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• v.62 no.5
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• pp.85-91
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• 2020

In order to calculate the Crop Water Stress Index (CWSI), it is necessary to collect weather data (air temperature, humidity, wind speed and solar radiation) and canopy temperature. However, it is not always available to have necessary data sets for CWSI calculation. Therefore, this study was aimed to develop an easy and simple CWSI equation (CWSI_EE) using only two data, air and canopy temperatures. Infrared sensors and weather sensors were installed on apple and peach trees and nearby a study area and every ten-minute data were collected from June to October in 2018 and 2019, respectively. A relationship between air-canopy temperature difference and CWSI was statistically analyzed and used to develop CWSI_EE using the three dimensional Gaussian model. The performance of CWSI_EE against original CWSI showed R² and NSE to 0.780 and 0.710 for apple trees and R² and NSE to 0.884 and 0.866 for peach trees. This study found that the level of crop water stress could be easily calculated using CWSI_EE with only air and canopy temperature data.

• Korean Journal of Plant Resources
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• v.33 no.6
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• pp.675-681
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• 2020

This study describes an efficient and stable droplet vitrification following cryopreservation of strawberry shoot tip (Fragaria × ananassa Duch.) accessions 'Massey' and 'MDUS3816'. The shoot tips were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.7M). Precultured explants were osmoprotected with loading solution (LS, C4) containing 17.5% glycerol and 17.5% sucrose for 40 min and exposed to dehydration solution (B1) containing 50% glycerol and 50% sucrose for 40 min at 25oC. Subsequently, the explants were transferred onto droplets containing 2.5 µL PVS3 on sterilized aluminum foils (4 cm× 0.5 cm) prior to direct immersion in liquid nitrogen (LN) for 1 h. The highest regrowth rate (%) in both the cultivars was obtained when the shoot tips were precultured with 0.3M sucrose for 30 h + 0.5M sucrose for 16 h at 25oC. The cryopreserved shoots tips exhibited 57.8 % recovery rate by culturing in NH₄NO₃-free MS medium supplemented with 3% sucrose, 1.0 g/L casein, 1.0mg/L GA₃, and 0.5 mg/L BA for 5 weeks and in MS medium supplemented with 0.5 mg/L GA₃ for 8 weeks. Variation was not observed in both of ploidy analysis and morphological investigation on plantlets of two accessions cryopreserved under variable preculture conditions.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.6
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• pp.648-657
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• 2015

Soil organic matter (SOM) plays an important role in the continuous production and environmental conservation in arable soils. In particular, the decomposition of organic matter in soil might promote soil organic matter and fertility due to the mineralization of N. In this study, to evaluate the effect of organic matter amendment on the C mineralization and N dynamic, $CO_2-C$ flux, extractable N and $N_2O$ emission were determined using closed chamber for 4 weeks at 10, 15, $20^{\circ}C$ of incubation temperature after the mixture of $2Mgha^{-1}$ rice straw compost and rye in sandy loam and clay loam. Regardless of soil texture, decomposition rates of rice straw compost and rye at $10{\sim}20^{\circ}C$ of incubation temperature ranged from 0.9 to 3.8% and 8.8 to 20.3%, respectively. Rye application in soil increased $NH_4-N$ and $NO_3-N$ content as well as the $N_2O$ emission compared to the rice straw compost. After incubation for 4 weeks, total C content in two soils was higher in rice straw compost than in rye application. In conclusion, application of rice straw compost and rye to soil was able to improve the soil organic matter and fertility. However, organic matter including the recalcitrant compounds like rice straw compost would be effective on the management of soil organic matter and the reduction of greenhouse gases in soil.

• Korean Journal of Plant Resources
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• v.31 no.6
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• pp.684-694
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• 2018

This study describes the successful establishment of a cryopreservation protocol for Citrus limon cultivars: 'Frost Eureka limon' and 'Cook Eureka limon', using a droplet-vitrification method. The shoot tips that were excised from in vitro grown seedlings of the two cultivars were preserved in liquid nitrogen (LN) and successfully regenerated into whole plants. Excised shoot tips were pre-cultured for 1 or 2 days in 0.3 M and 0.5 M sucrose solutions at $25^{\circ}C$ and incubated in a loading solution (LS) composed of 17.5% glycerol + 17.5% sucrose in Murashige and Skoog (MS) medium for 40 min at $25^{\circ}C$. Prior to direct immersion in LN for 1 h, the shoot tips were dehydrated with plant vitrification solution 2 (PVS2) at $0^{\circ}C$ or PVS3 at $25^{\circ}C$. The frozen shoot tips were re-warmed and unloaded with 1.2 M sucrose in $\text\tiny{^1/_2}$ MS for 30 min at $25^{\circ}C$. Shoot tips were post-cultured overnight on survival medium and then micrografted onto 'trifoliate orange' (Poncirus trifoliate (L.) Raf. seedling rootstocks for recovery and to produce whole plants. The highest regrowth rates were 53.5% and 50.3% for cryopreserved shoot tips of 'Frost Eureka limon' and 'Cook Eureka limon', respectively, when pre-cultured in 0.3 M and 0.5 M sucrose concentrations in a sequencing manner, with LS and treated with PVS2 for 60 min at $0^{\circ}C$. We also investigated whether the ammonium ion concentration on post-culture medium affected the viability of the cryopreserved Citrus shoot tips. The viability of cooled samples, following culturing on woody plant media (WPM) containing $\text\tiny{^1/_4}$ ammonium nitrate overnight before micrografting, was the highest (70.3%) in 'Frost Eureka limon'. The study described here is a cost-effective and safe method to conserve Citrus fruit cultivars, for the improvement and large-scale multiplication of fruit plants and for breeding disease resistance.