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Glucose Effects on Cell Growth, Antibody Production, and Cell Metabolism of Hybridoma Cells (Hybridoma 세포의 세포성장, 항체생산 및 세포대사에 미치는 Glucose의 영향)

  • ;Shaw S.Wang
    • KSBB Journal
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    • v.10 no.3
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    • pp.323-334
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    • 1995
  • The effects of glucose on cell growth kinetics, monoclonal antibody productivity, and cell metabolism or hybridoma cells were investigated. The mouse-mouse hybridoma cell line VIII H-8 producing mouse IgG2a was used as a modal system. Glucose showed substrate inhibition type dependence on specific growth raie. The maximum cell density increased as initial glucose concentration increased up to 4 g/$\ell$. Glucose showed a strong influence on cell death kinetics, and an inverse relationship between specific death rate and glucose concentration was found. Cell viability and monoclonal antibody production increased as initial glucose concentration increased. The specific glucose consumption rate increased with glucose concentration, and cumulative specific lactate production rate increased with increasing initial glucose concentration. The overall kinetics of ammonium ion production was almost invariant with respect to initial glucose concentration, while the cumulative specific ammonium ion production rate was dependent on initial glucose concentration.

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A Literature Review of Team Effectiveness on Hospital Nursing Units (간호단위의 팀 효과성에 대한 고찰)

  • Kim, Se Young;Kim, Jong Kyung;Jung, Myun Sook;Kim, Eun Kyung;You, Sun Ju
    • Journal of Korean Academy of Nursing Administration
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    • v.23 no.1
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    • pp.18-31
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    • 2017
  • Purpose: The purpose of this study was to analyze literature related to nursing team effectiveness and to summarize the definition variables included, measurement tools, and findings. Basic data on operation and research for team effectiveness in nursing units was sought. Methods: A literature search was performed to identify all studies published between 2003 and 2016 from periodicals indexed in PUBMED, CINAHL, SCIENCE DIRECT, RISS, KISS, and NAL databases. The following keywords were used in the search: 'team effectiveness', 'nurse', 'nursing', and 'hospital'. Ten studies were analyzed. Results: The analysis included domestic and international literature on nursing team effectiveness. The foreign literature included studies of various organizations including nurses, use of various research tools, leadership programs and/or new nursing delivery systems. In the Korean studies, most of the research on team effectiveness surveyed nurses on team satisfaction, team commitment, and team performance in general nursing units, operating rooms, and intensive care units. Conclusion: The findings show the necessity to develop a definition of team effectiveness that can be accepted comprehensively in nursing organizations in Korea. The definition should reflect team effectiveness that includes all cooperating units not only nurses of the nursing unit but also all other related health care teams.

A Study on the formativeness of the Minimal Art represented in the second half 20th century fashion (20세기 후반 패션에 나타난 미니얼 아트의 조형성에 관한 연구)

  • 이효진
    • Journal of the Korean Society of Costume
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    • v.29
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    • pp.23-38
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    • 1996
  • The purpose of this study was to analyze the characteristics of the Minimal Art represented in the second half 20th century fashion. All the mode of dress worn this century have developed from the stimulus of a chang-ing society fired by new discoveries and a zest for the new and different. It would appear that even more dramatic changes will soon occur in our clothing styles the effects of which will be farreaching. Many of today's styles intended to dress our psyche rather than our bodies and this if fully understood by the new designers whose influ-ence will become increasingly more potent as we approach the new century. The highly developed material civilization resulted in human alienations environmental distruption, l By this fact human being of the 20th century was to overcome social com-plexity. Accordingly their search for simplicity was interested in the 60's style. Especially the formativeness of the 60s style was repre-sented in the second half 20th century fashion. In this paper it was focused that how the characteristics of the Minimal Art was expressed in the second half 20th century fashion. minimalism was a quest for basic elements repesenting the fundamental esthetic values of art. without regard to issues of content. At its most extreme it reduced art not ot an eter-nal essence but to an arid simplicity. "Primary Structure" the most suitable name suggested for this type conveys its two salient characteristics : extreme simplicity of shapes and a kinship with architecture. minimalist works are charcterized by huge dimensions coldness and absolute aesthetic neutrality. minimalist artists ambition is to de-fine through the most rudimentary materials such as plywood galvanized iron aluminum plastic and wood a new order of the space. The environment is just as important as the object itself. Similary the use of new tough materials. such as vinyl metal and plastic at late half of the 20th century fashion related to the formativeness of the Minimal Art. And the style of 20th fashion was holded the internal meanings in common the formativeness of the Minimal Aet thorough the various texture pat-tern silhouette etc.uette etc.

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Characterization of Extended-Spectrum-$\beta$-Lactamase Genotype TEM, SHV and CTX-M from Clinical Isolates of Klebsiella pneumoniae and Comparison with Antibiotic Susceptibility Test

  • Kim Yun-Tae;Oh Kwang-Seok;Choi Seok-Cheol;Kim Tae-Un
    • Biomedical Science Letters
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    • v.11 no.3
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    • pp.389-396
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    • 2005
  • Resent studies have reported increased isolation of extended-spectrum $\beta-lactamase$ (ESBL) producing strains at several hospital in Korea. We studied to investigate the isolation rates of ESBL strains from clinical isolates of Klebsiella pneumoniae and to characterize differences in types using analyses of genotyping and antibiotic susceptibility test. Antibiotic susceptibility test with confirmation of ESBL by double disk synergy test was performed on the 54 ESBL strains of Klebsiella pneumoniae from a hospital in Busan. Transfer of resistant gene in ESBL strains resistant to 3rd generated antibiotics was confirmed by transconjugation test using E. coli $RG176^{nal(r)}$. blaTEM, blaSHV, blaCTX-M genes were detected by PCR. ESBL producing strains had 100% of resistant rate to ampicillin, azteronam, cefazolin, cefepime and ceftriaxone ($\beta-lactam$ antibiotics). Forty strains of bla TEM$(74\%)$, 41 strains of bla SHV $(76\%)$, 23 strains of bla CTX-M $(43\%)$ were found, respectively. The strains had one or more genes. They had high resistant rates to $\beta-lactam$ antibiotics including cephalosporin. The resistant rates of strains with multiple resistant genes were higher than those of strains with single resistant gene.

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도서분류자동화를 위한 지식베이스의 설계에 관한 연구

  • 이경호
    • Journal of Korean Library and Information Science Society
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    • v.18
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    • pp.139-192
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    • 1991
  • Though the computer has become deeply entrenched as the major tool in information processing(library works), it may be obvious that automatic book classification techniques ate still under experimentation, and the techniques have not yet been tested against the criterion of usefulness. The purpose of this study is to design of knowledge base for automatic book classification which can be put to use in library operation, and to present a methodology of application of the automatic classification into the library. Since the enumerative classification schemes which are existing are manual systems, it cannot be applied to the automatic classification, the principle of faceted classification based on concept analysis is brought in and studied. The result of this study are summarized as follows : 1. The design of knowledge base confined the field of agriculture and medicine. 2. If title is entered by the computer keyboard it will be searched in knowledge base, and then be classified by the principle of automatic classification. 3. Program flowcharts are designed as a bases of classification procedures for automatic subject recognition and classification. 4. 283 books in agriculture, 196 books in medicine were drawn at random from Taegu University Library and Young-Nal Medical Center Library respectively. 5. The experiment of automatic classification is performed 143 books in agriculture 166 books in medicine except for other subject books. 6. It was proved that automatic book classification is possible by design of knowledge base. In addition the expected values from design of knowledge base for automatic book classification are as follows : 1. The prompt and accurate process of classification is possible. 2. Though some title is classified in any library, it can be classified the some classification number by a program. 3. The user can retrieve the classification codes of books for which he or she wants to search through the computer. 4. Since the concept coordination method is employed the representing of a multisubject concept is make simple. 5. By performing automatic book classification the automation of total system can be achieved. 6. The efficient international information transfer will be advanced since all the institution maintain unified classification number.

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A Novel Equalization Method of Multiple Transceivers of Multiple Input Multiple Output Antenna for Beam-farming and the Estimation of Direction of Arrival (빔조향 및 전파도래각 추정을 위한 새로운 다중입력 다중출력 안테나 송수신부 구성방법)

  • 이성종;이종환;염경환;윤찬의
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.13 no.3
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    • pp.288-300
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    • 2002
  • In this paper, a novel method of equalization of RF transceivers is suggested for MIMO(Multiple Input Multiple Output) antenna actively studied for high speed data transmission in the recent IMT-2000 system. The core of suggestion is in equalizing the transfer characteristics of multiple transceivers using feedback and memory during the predefined calibration time. This makes it possible to weight the signals in the intermediate frequency, which is easier in the application of recently developed DoA(Direction of Arrival) algorithms. In addition, the time varying optimum cell formation according to traffic is feasible by antenna beam-forming based on the DoA information. The suggested method of equalizing multiple transceivers are successfully verified using envelope simulation. two outputs. This paper is concerned with the diagnosis of multiple crosstalk-faults in OSM. As the network size becomes larger in these days, the convent.nal diagnosis methods based on tests and simulation be.me inefficient, or even more impractical. We propose a simple and easily implementable alg?ithm for detection and isolation of the multiple crosstalk-faults in OSM. Specifically, we develop an algorithm for isolation of the source fault in switc.ng elements whenever the multiple crosstalk-faults are.etected in OSM. The proposed algorithm is illustrated by an example of 16$\times$16 OSM.

Study on the Attributive Channel Theory of Herbal Medicine by the Pharmacodynamic Research of 1-131 Labelled Hesperetin (Hesperetin의 I-131 표지 및 생체(生體) 분포(分布) 분석(分析)을 통한 귀경(歸經) 연구)

  • Kim, Sung-Kyu;Ham, In-Hye;Bu, Young-Min;Kim, Ho-Cheol;Cho, Jung-Hyuck;Choi, Ho-Young
    • The Korea Journal of Herbology
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    • v.23 no.1
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    • pp.117-125
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    • 2008
  • Objectives : This study was to verify the Attributive Channel theory of herbal medicine. Methods : [$^{131}I$]iodohesperetin was synthesized, separated, and refined from hesperetin, the major component of Citrus species, followed by observing the biodistribution in an organism of C57 BL/6 mice with and without Lewis Lung Carcinoma. Results : Iodohesperetin 27.5 mg was obtained through column cliromatography after a reaction with 50 mg of Hesperitin and 8 mg of Nal. The radiochemical yield of [$^{131}I$]iodohesperetin synthesis was 25 % when checked with Radio TLC chromatography. [131I]iodohesperetin was most largely distributed in the stomach, lung and liver of C57BL/6 mouse. The highest %ID/g in stomach was 40 min, in lung and liver was 20 min after injection. The % ID/g of tumor tissue was comparable with that of blood. Conclusions : The fact that [$^{131}I$]iodocurcumin was most largely distributed in the stomach, lung and liver was related with the Attributive Channel theory. And there was no significant finding related to tumor cells.

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Study on the Dosimetry and Assessment of Terrestrial Radiation Exposure (지각 방사선에 의한 피폭선량측정 및 해석)

  • Jun, Jae-Shik;Oh, Hi-Peel;Ha, Chung-Woo
    • Journal of Radiation Protection and Research
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    • v.15 no.2
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    • pp.87-100
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    • 1990
  • For the quantitative evaluation and assessment of radiation exposure from terrestrial component of natural environmental radiation, successive thermoluminescence dosimetry and periodical in-situ gamma ray spectrometry were carried out for a period of 24 months. LiF PTFE dise TLDs and $3&{\phi}{\times}3'$cylindrical NaI(Tl)scintill ation detector in association of portable multichannel analyzer (4096 ch) were used in this study. The doses measured were evaluated and assessed in terms of effective dose equivalent. As a concomitant output, the dose equivalent due to ionizing component of cosmic ray was able to be evaluated. According to the results obtained in terms of variance weighted mean, the annual effective dose equivalents of terrestrial gamma ray and cosmic ray ionizing component in Taejeon area came out to be $564{\pm}4\;{\mu}Sv(64.8{\pm}0.5nSv{\cdot}h^{-1}$ and $300{\pm}2\;{\mu}Sv(34.3{\pm}0.2nSv{\cdot}h^{-1}$, respectively, which are reasonable comparably with that appeared in UNSCEAR Report[28]as per caput annual effective dose equivalent in 'areas of normal background radiation'.

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Dosimetrical Analysis of Reactor Leakage Gamma-rays by Means of Scintillation Spectrometry

  • Jun, Jae-Shik
    • Nuclear Engineering and Technology
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    • v.5 no.4
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    • pp.291-309
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    • 1973
  • Exposure rates due to leakage gamma-rays from operating reactors TRIGA Mark II and III were measured in a horizontal plane by means of scintillation spectrometry using a 3"$\times$3" cylindrical Nal(T1) detector associated with a 400 channel pulse height analyzer under varied conditions of reactor operation. In determining exposure rate due to the leakage gamma-rays at each point of measurement, Moriuchi's spectrum-exposure rate conversion theory was applied instead of using conventional responce matrix method which necessitates very complicated procedures to convert a spectrum into exposure rate. The results show that a basic pattern of "typical" spectrum of the reactor leakage gamma-rays is neither affected by thermal output of the reactor, nor influenced by overall attenuation in radiation intensity. It was indicated that he attenuation of the leakage gamma-rays in air in terms of exposure rate as a whole follows an exponential law, and the total exposure rate due to the leakage gamma-rays at a certain point is nearly proportional to thermal output of the reactor. The complexity in spectrum measured for a movable core reactor, TRIGA Mark III, was analyzed through spectrum resolution, and proper judgement of the leakage gamma-rays in a complex spectrum was discussed.ctrum was discussed.

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Influence of Mentoring Type and Mentoring Function on the Organizational Commitment among the Security Organization (시큐리티조직의 멘토링유형과 멘토링기능이 조직몰입에 미치는 영향)

  • Kim, Chan-Sun
    • Convergence Security Journal
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    • v.11 no.5
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    • pp.21-29
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    • 2011
  • This study is to investigate how security organizational mentoring type and mentoring function affects organizatio nal commitment. This study had selected security from 5 different security organizations of 2010 which are located i n capital area. Using judgment sampling method, 198 security were drawn for the final study. The survey used in th is study is composed of 37 questions. To practice frequency analysis, factorial analysis, reliability analysis and regre ssion, a program called SPSSWIN 18.0 was used. The value of Cronbach's ${\alpha}$, which shows the reliability of the stud y, appeared to be over .622. The result is: First, security organizational mentoring type affects mentoring function. T hat is, when a systematic mentoring is activated, friendship protecting function, career managing function and sociopsychological function are promoted. Second, security organizational mentoring type affects organizational commitmen t. That is, when a systematic mentoring is activated, organizational commitment is promoted. Third, security organiz ational mentoring function affects organizational commitment. That is, increased friendship protecting function, career managing function, socio-psychological function and role model function results in increased organizational commitment.