• 제목/요약/키워드: N4 phage

검색결과 27건 처리시간 0.024초

Lactobacillus casei의 Bacteriophage내성돌연맥리균분리 (Development of Phage-resistant Mutants from Lactobacillus casei)

  • 강국희;이경화;박기문;유익제;김영창
    • 한국미생물·생명공학회지
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    • 제10권3호
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    • pp.217-222
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    • 1982
  • 발효유 starter로서 사용하고 있는 유산간균 L. casei YIT 9018을 NTG로 처리하여 4종류의 phage(J$_1$, Tk93, PD5, $K_1$)에 대하여 저항성을 나타내는 8 개의 돌연변이균주(PR5, PR6, PR35, PR38, PR46, PR47, PR48)를 분리하여, 산생성력, 생육, 당발효성, 내균성을 시험하였다. Phage저항균주 8개중에서 특히 PR38은 4 종류의 phage에 대하여 4 개월동안 저항성을 유지하였고, 생육속도, 산생성력 등에 있어서 parent strain과 비교할때, 유의적인 차이가 없었고 (P>0.01), 인공위액에서의 내균성은 parent strain보다 약한 것으로 나타났다. 당발효성에 있어서는 양자 사이에 차이가 없었다. 본 실험에서 분리한 PR38은 발효유제조시에 phage가 감염되면, Parent strain 대용으로 사용할 수 있는 가능성이 제시되었다.

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Studies on Lytic, Tailed Bacillus cereus-specific Phage for Use in a Ferromagnetoelastic Biosensor as a Novel Recognition Element

  • Choi, In Young;Park, Joo Hyeon;Gwak, Kyoung Min;Kim, Kwang-Pyo;Oh, Jun-Hyun;Park, Mi-Kyung
    • Journal of Microbiology and Biotechnology
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    • 제28권1호
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    • pp.87-94
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    • 2018
  • This study investigated the feasibility of the lytic, tailed Bacillus cereus-specific phage for use in a ferromagnetoelastic (FME) biosensor as a novel recognition element. The phage was immobilized at various concentrations through either direct adsorption or a combination of 11-mercapto-1-undecanoic acid (11-MUA) and [N-(3-dimethylaminopropyl)-N'-carbodiimide hydrochloride and N-hydroxysuccinimide (EDC/NHS)]. The effects of time and temperature on its lytic properties were investigated through the exposure of B. cereus (4 and 8 logCFU/ml) to the phage (8 logPFU/ml) for various incubation periods at $22^{\circ}C$ and at various temperatures for 30 and 60 min. As the phage concentration increased, both immobilization methods also significantly increased the phage density (p < 0.05). SEM images confirmed that the phage density on the FME platform corresponded to the increased phage concentration. As the combination of 11-MUA and EDC/NHS enhanced the phage density and orientation by up to 4.3-fold, it was selected for use. When various incubation was conducted, no significant differences were observed in the survival rate of B. cereus within 30 min, which was in contrast to the significant decreases observed at 45 and 60 min (p < 0.05). In addition, temperature exerted no significant effects on the survival rate across the entire temperature range. This study demonstrated the feasibility of the lytic, tailed B. cereus-specific phage as a novel recognition element for use in an FME biosensor. Thus, the phage could be placed on the surface of foods for at least 30 min without any significant loss of B. cereus, as a result of the inherent lytic activity of the B. cereus-specific phage as a novel recognition element.

Mannose permease가 변형된 대장균 변이주에 대한 coliphage N4 감염의 저해 (Ingibition of coliphage N4 infection to escherichia coli mutant defective in mannose permease)

  • 김기태;유욱준
    • 미생물학회지
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    • 제25권3호
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    • pp.184-188
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    • 1987
  • Evidences that the mannose permease of Escherichia coli mediates the infection of N4 in early steps, were obtained as follows. First, A mutant strain of Escherichia coli which was resistant to both wild type N4 and lambda whose genome is Charon 4A containing human genomic fragments in its EcoR I site, could not use mannose efficiently. Second, N4 could not infect pel mutant strains which lack one or all of intact components of mannose permease. However, unknown alterations in N4 made it possible for the phage to infect pel mutant of E. coli. It also turned out to be clear that the receptor of N4 was different from that of lambda.

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대장균의 회분식 발효에 의해 생산된 덱스트란 결합 파아지를 활용한 설탕 제조공정 오염 검출 (Detection of Sugar Process Contamination Using Dextran Binding hages Produced by Batch Fermentation of Escherichia coli)

  • 김두운
    • 한국식품저장유통학회지
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    • 제15권5호
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    • pp.617-621
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    • 2008
  • Sequential passes through $Sephadex^{TM}$ columns were used to select phages that displays ligands for dextran ($\alpha$-1,6 linked linear chains) from a phage antibody library. Those phages that bound to the $Sephadex^{TM}$ in each iteration were replicated in E. coli. A phage preparation isolated on the third round selection produced 5.4 nephelos turbidity units (NTU) in a dextran specific immunonephelometric assay, a 2.2 fold higher value than the phage preparation from the first round selection. This phage gave $72\;{\pm}\;10$ normalized intensity (N.I.) in a dip-stick assay against high molecular size dextran (T2000, $2\;{\times}\;10^6) and significantly lower color ($30\;{\pm}\;6$ N.I.) against low molecular size dextran (T10, $10^4$). The presence of an Fab insert in each of these phages was confirmed using a $\beta-galactosidase linked assay and polymerase chain reaction.

Phage Assembly Using APTES-Conjugation of Major Coat p8 Protein for Possible Scaffolds

  • Kim, Young Jun;Korkmaz, Nuriye;Nam, Chang Hoon
    • Interdisciplinary Bio Central
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    • 제4권3호
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    • pp.9.1-9.7
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    • 2012
  • Filamentous phages have been in the limelight as a new type of nanomaterial. In this study, genetically and chemically modified fd phage was used to generate a biomimetic phage self-assembly product. Positively charged fd phage (p8-SSG) was engineered by conjugating 3-aminopropyltriethoxysilane (APTES) to hydroxyl groups of two serine amino acid residues introduced at the N-terminus of major coat protein, p8. In particular, formation of a phage network was controlled by changing mixed ratios between wild type fd phage and APTES conjugated fd-SSG phage. Assembled phages showed unique bundle and network like structures. The bacteriophage based self-assembly approach illustrated in this study might contribute to the design of three dimensional microporous structures. In this work, we demonstrated that the positively charged APTES conjugated fd-SSG phages can assemble into microstructures when they are exposed to negatively charged wild-type fd phages through electrostatic interaction. In summary, since we can control the phage self-assembly process in order to obtain bundle or network like structures and since they can be functionalized by means of chemical or genetic modifications, bacteriophages are good candidates for use as bio-compatible scaffolds. Such new type of phage-based artificial 3D architectures can be applied in tuning of cellular structures and functions for tissue engineering studies.

Bacollis cereis의 RK-용원파아지에 관한 연구 (Studies on the RK-temperate phage of bacillus cereus)

  • 이태우
    • 미생물학회지
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    • 제23권2호
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    • pp.129-137
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    • 1985
  • The RK-temperate phage which infected with Bacillus cereus was isolated and the characters were investigated. The induction of RK-temperate phage from host bacterium attained by ultraviolet light irradiation (15W, 30cm, 30-120sec) and mitomycin C treatment (0.2-2 ug/ml). The host range of RK-temperate phage was not revealed with lysogenic and related strains of B. cereus. But B. cereus(PS) 352 which obtained by N-nitrosoguanidine treatment (1,000{$\mu}g/ml)$ to phage infected with host bacteria was sensitive bacteria of RK-temperate phage. RK-temperate phage was stabilized at the condition of nutrient broth (pH 7-8), Tris-buffer (pH 7-8) and ammonium buffer (pH 8-9) and Sorensen's phosphate buffer (pH 6-7), but unstabilized at other salt solutions and pH range. Also, thermostability was to $45^{\circ}C$ but unstabilized at above $50^{\circ}C$. At RK-temperate phage, the measurment values of head, neck, mid tail and end tail were 59nm, $9{\times}16nm,\;10{\times}189nm,\;and\;10{\times}14nm$ respectively. The morphology of head was regular polyhedron, and the end tail was coneate form. On the one hand, the number of capsid protein layer of tail were consist of 4, 35, and 1 at neck, mid tail, and end tail, respectively. RK-temperate phage was identified with DNA phage and G+C contents were 38.63. The latent time of RK-temperate phage was 30 minutes and the burst size was 70-80. And the host bacteria was lysed in case of multi-infection, above moi 1.

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미생물에 의한 벤제노이드의 분해 (Degradation of Benzenoids by Microorganisms)

  • 권영명;하영칠
    • 미생물학회지
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    • 제16권2호
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    • pp.79-89
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    • 1978
  • The RK-temperate phage which infected with Bacillus cereus was isolated and the characters were investigated. The induction of RK-temperate phage from host bacterium attained by ultraviolet light irradiation (15W, 30cm, 30-120sec) and mitomycin C treatment (0.2-2 ug/ml). The host range of RK-temperate phage was not revealed with lysogenic and related strains of B. cereus. But B. cereus(PS) 352 which obtained by N-nitrosoguanidine treatment(1,000.$\mu$g/ml) to phage infected with host bacteria was sensitive bacteria of RK-temperate phage. RK-temperate phage was stabilized at the condition of nutrient broth (pH 7-8), Tris-buffer (pH 7-8) and ammonium buffer (pH 8-9) and Sorensen's phosphate buffer (pH 6-7), but unstabilized at other salt solutions and pH range. Also, thermostability was to 45.deg.C but unstabilized at above 50.deg.C. At RK-temperate phage, the measurment values of head, neck, mid tail and end tail were 59nm, 9*16nm, 10*189nm, and 10*14nm respectively. The morphology of head was regular polyhedron, and the end tail was coneate form. On the one hand, the number of capsid protein layer of tail were consist of 4, 35, and 1 at neck, mid tail, and end tail, respectively. RK-temperate phage was identified with DNA phage and G+C contents were 38.63. The latent time of RK-temperate phage was 30 minutes and the burst size was 70-80. And the host bacteria was lysed in case of multi-infection, above moi 1.

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Lactobacillus casei YIT 9018로부터 Prophage cured strain의 분리 및 특성 (Isolation and Characterization of Prophage cured strain derivatives from Lactobacillus casei YIT 9018)

  • 이정준;김경태;백영진
    • 한국미생물·생명공학회지
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    • 제18권3호
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    • pp.215-220
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    • 1990
  • L.casei YIT 9018균주에 N-methyl-N'-nitro-N-nitrosoguanidine을 처리하여 thermoinducible mutants를 분리하였고, 이 균주를 $42^{\circ}C$에서 30분 동안 열처리하여 prophage가 cured된 균주 L.casei HYM 1213과 L.casei HYM 4024를 분리하였다. Prophage cured strain L.case HYM 1213과 L.casei HYM 4024는 temperate phage $\phi$Fsw에 대해 지시균으로서 능력을 가지고 있었으며, 어떤 온도에서도 temperate phage $\phi$FSW가 검출되지 않았다. 이들의 생리적 특성을 모균주인 L.caseiYIT 9018과 비교한 결과 L.casei HYM 1213 균주는 균의 증식, pH변화, 산생성능력, 당 발효능력에서 모균주와 유사한 것으로 나타났으나, L.casei HYM 4024균주는 pH변화와 산생성능력이 모균주에 비해 미약한 것으로 나타났다. Plasmid DNA는 두 균주 모두 모균주와 동일한 size를 보여주여, prophage가 cured되었어도 plasmid DNA에는 손실을 가져오지 않았음을 알 수 있었다.

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N4에 대해 내성을 나타내는데 필요한 rtn 유전자의 부위 (The DNA region of rtn gene essential for resistance against N4 infection)

  • 이동환;유선미;황의욱;이영훈;채건상
    • 미생물학회지
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    • 제29권5호
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    • pp.290-295
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    • 1991
  • N4 phage, which infects E. coli K-12 strains, could not infect E. coli K-12 strains containing rtn(resistant to N4) gene on plasmids, which was isolated from Proteus vulgaris ATCC 13315. The region of rtn gene for Rtn phenotype was reduced to the 1.7 kb HincII-AccI fragment, and rtn gene seemed to have its own promoter. This putative promoter was present in 107 bp HindII-DraI fragment, and known to be functional in E. cole K-12, which is supported by the fact that phenotype of a subclone, pRMG103A1B which does not contain the 107 bp fragment, was dependent on the existance of a functional promoter in the upstream of rtn gene, and that the 107 bp fragment had promoter activity when located in the upstream of structural gene of galactodinase of E. coli. The promoter-bearing fragment contains two overlapping putative promoter sequences, both of which show a fit in eight of twelve nucleotides with consensus sequences of E. coli promoters at the -35 and -10 regions.

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Identification of Six Single-Strand Initiation (ssi) Signals for Priming of DNA Replication in Various Plasmids

  • Jeong, Jin-Yong;Seo, Hak-Soo;Kim, Ho-Yeon;Cho, Moo-Je;Bahk, Jeong-Dong
    • BMB Reports
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    • 제28권4호
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    • pp.336-341
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    • 1995
  • Using a mutant M13 phage derivative lacking a great part of the complementary strand synthesis origin, we identified six single-strand initiation (ssi) signals for DNA replication in pACYC184, pLG214, pGKV21, and pDPT270 plasmids, and named them $ssiA_{YC}$, $ssiA_{LG}$, $ssiB_{LG}$, $ssiA_{KV}$, $ssiA_{PT}$, and $ssiB_{PT}$, respectively. Two of them were from pDPT270, one from downstream the on of pACYC184, two from pLG214, one from upstream the plus origin of pGKV21. Introduction of these ssi signals into the deleted $ori_c$ site of a mutant filamentous M13 phage ($M13{\Delta}lac182$) resulted in the restoration of growth activity of this phage. These ssi signals were classified into a number of groups on the basis of sequence similarity. $ssiA_{YC}$ and $ssiA_{LG}$ show extensive sequence homology to the n'-site (primosome assembly sites) of ColE1, whereas $ssiB_{PT}$ is homologous to the n'-site of ${\Phi}X174$. $ssiA_{PT}$ belongs to G4-type ssi signals which require only dnaG primase and SSB protein for the priming of replication. In addition, possible biological roles of these ssi signals are discussed.

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