• Title/Summary/Keyword: N.D.C method

Search Result 794, Processing Time 0.032 seconds

Exchange Interaction In $Y_{3-x}Ce_xFe_5O_{12}$ Fabricated Using a Sol-gel Method (Sol-gel 법으로 만든 $Y_{3-x}Ce_xFe_5O_{12}$ 의 초교환상호작용 연구)

  • 금준식;김삼진;김철성;이보화
    • Proceedings of the Korean Magnestics Society Conference
    • /
    • 2002.12a
    • /
    • pp.108-109
    • /
    • 2002
  • 자성 garnet(YIG)의 현재 가장 널리 쓰이는 microwave 자성재료 중 하나이며 자기적 손실이 적은 특성을 가지고 있다고 보고되어 지고 있다.[1] Microwave 소자로 응용하기 위해서는 포화자화값 (M$_{s}$ ), 보자력 (H$_{c}$), Neel 온도 (T$_{N}$)등을 제어하는 기술을 요구되어진다. 이러한 자성재료인 Garnet의 결정 내에는 octahedral-16a과 tetrahedral-24d 그리고 dodechahedral-24c의 세 개의 부격자가 있다. 이러한 부격자들에 치환되는 이온에 따라 자기적 교환 상호작용이 달라지게 된다. (중략)

  • PDF

Vibration Serviceability Evaluation for Pedestrian of Concrete Cable-stayed Bridge by Experimental Method (실험적 방법에 의한 콘크리트 사장교의 보행자 중심 진동사용성 평가)

  • Kang, Sung-Hoo;Choi, Bong-Hyun;Park, Sun-Joon
    • Journal of the Korean Society of Hazard Mitigation
    • /
    • v.11 no.2
    • /
    • pp.59-66
    • /
    • 2011
  • In this study, the vibration serviceability of pedestrian by travelling vehicles on the cable-stayed bridge with concrete tower was studied. Experiment variables were considered travelling speed of vehicles, pavement state of asphalt on the deck and weight of vehicles, preferentially. Especially, pavement grade states were considered by A and C grades by BMS (Bridge Management System) standard. The incremental ratio extent of vibration acceleration responses, asphalt pavement grade C over A, was construed to 1.23~1.43. Only, these results are valid within extent of the Scaled-Weight 228.0~1161.9 km/h kN. The vibration equations for acceleration responses prediction of bridge deck were proposed into three types, reliability 50%, 90%, 95% respectively. These equations can consider asphalt pavement grade, and the vehicle's weight and travelling velocity, which are the source of vibration, are combined into the term called, 'Scaled Weight'.

N-oleoyl-D-erythro-sphingosine-based Analysis of Ceramide by High Performance Liquid Chromatography and Its Application to Determination in Diverse Biological Samples

  • Lee, Youn-Sun;Choi, Heon-Kyo;Yoo, Jae-Myung;Choi, Kyong-Mi;Lee, Yong-Moon;Oh, Sei-Kwan;Kim, Tack-Joong;Yun, Yeo-Pyo;Hong, Jin-Tae;Okino, Nozomu;Ito, Makoto;Yoo, Hwan-Soo
    • Molecular & Cellular Toxicology
    • /
    • v.3 no.4
    • /
    • pp.273-281
    • /
    • 2007
  • Ceramide is involved in cell death as a lipid mediator of stress responses. In this study, we developed an improved method of ceramide quantification based on added synthetic ceramide and thin layer chromatography (TLC) separation, and applied to biological samples. Lipids were extracted from samples spiked with N-oleoyl-D-erythro-sphingosine ($C_{17}$ ceramide) as an internal standard. Ceramide was resolved by TLC, complexed with fatty-acidfree bovine serum albumin (BSA), and deacylated by ceramidase (CDase). The released sphingosine was derivatized with o-phthalaldehyde (OPA) and measured by high performance liquid chromatography (HPLC). The limit of detection for ceramide was about 1-2 pmol and the lower limit of quantification was 5 pmol. Ceramide recovery was approximately 86-93%. Ceramide concentrations were determined in biological samples including cultured cells, mouse tissues, and mouse and human plasma. TLC separation of ceramide provides HPLC chromatogram with a clean background without any interfering peaks and the enhanced solubility of ceramide by BSAceramide complex leads to the increased deacylation of ceramide. The use of an internal standard for the determination of ceramide concentration in these samples provides an accurate and reproducible analytical method, and this method can be applicable to diverse biological samples.

Study on the Direct Bonding of Silicon Wafers by Cleaning in $HNO_3:H_2_O2:HF$ (HNO$_3:H_2O_2$ : HF 세척법을 이용한 실리콘 직접 접합 기술에 관한 연구)

  • Joo, C.M.;Choi, W.B.;Kim, Y.S.;Kim, D.N.;Lee, J.S.;Sung, M.Y.
    • Proceedings of the KIEE Conference
    • /
    • 1999.07g
    • /
    • pp.3310-3312
    • /
    • 1999
  • We have studied the method of silicon direct bonding using the mixture of $HNO_$, $H_2O_2$, and HF chemicals called the controlled slight etch (CSE) solution for the effective wafer cleaning. CSE, two combinations of oxidizing and etching agents, have been used to clean the silicon surfaces prior to wafer bonding. Two wafers of silicon and silicon dioxide were contacted each other at room temperature and postannealed at $300{\sim}1100^{\circ}C$ in $N_2$ ambient for 2.5 h. We have cleaned silicon wafers with the various HF concentrations and characterized the parameters with regard to surface roughness, chemical nature, chemical oxide thickness, and bonding energy. It was observed that the chemical oxide thickness on silicon wafer decreased with increasing HF concentrations. The initial interfacial energy and final energy postannealed at $1100^{\circ}C$ for 2.5h measured by the crack propagation method was 122 $mJ/m^2$ and 2.96 $mJ/m^2$, respectively.

  • PDF

Studies on Pathogenicity of Nosema bombycis Naegeli to Parents and Hybrid in the Silkworm (Bombyx mori L.) (원종 및 교잡종에 대한 미립자의 병원성에 관한 연구)

  • 김종수;김문협;채수군;임종성
    • Journal of Sericultural and Entomological Science
    • /
    • v.14 no.2
    • /
    • pp.81-92
    • /
    • 1972
  • Studies on Pathogenicity of Nosema bombycis Naegeli are summarized as follows: 1. The mortality of the parents, Jam 103 and Jam 104, is remarkbly higher than that of the hybrid, Jam 103$\times$Jam 104, whereas there is no difference in the mortality between the parents. 3. In the mortality of the pathogen-concentration, it is increased in order of the following concentrations inoculated, 10$^{8}$ , 10$^{7}$ , 10$^{8}$ and 10$^{5}$ /ml. 3. In the mortality of each instar, it is high in order of 5th, 4th, 3rd, and 2nd instar. 4. In the interaction between the mortalities of the varieties and the concentrations, 1) The mortality shows no differences between the parents and the hybrid in the high concentration of 10$^{8}$ /ml. 2) The mortality of the hybrid is lower than that of the parents in the low concentration of 10$^{5}$ /ml, whereas no difference is found between the parents. 3) The interaction appears at the same level in the middle concentration of 10$^{6}$ /ml to the parents and of 10$^{7}$ /ml to the hybrid. 5. It was pointed out that active immunity depends upon the volume of antigen injection, immunizing period, and injection intervals. In this experiment, it is noticed that the optimum volume of injection is above 20ml and D is the best one of the four treatment (A.B.C.D.). 6. The immune sera indicate such a 12,800 high titer in the indirect method can be obtained from the D immunizing method. Silkworm tissues and N. bombycis spores show self-fluorescence, but it is able to distinguish it from the F.I.T.C. by using the U.V. filter. 7. The midgut epithelium is examined to be the first site of the tissues which are penetrated into and multiplied by the inoculation of the pathogen per os.

  • PDF

Piezoelectric and Dielectric Properties of Low Temperature Sintering (Na,K,Li)(Nb,Sb,Ta)O3 Ceramics Doped with CuO (CuO 첨가된 저온소결 (Na,K,Li)(Nb,Sb,Ta)O3계 세라믹스의 압전 및 유전 특성)

  • Lee, Gwang-Min;Yoo, Ju-Hyun;Lee, Jie-Young
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
    • /
    • v.28 no.4
    • /
    • pp.229-233
    • /
    • 2015
  • In this paper, in order to develop outstanding Pb-free piezoelectric composition ceramics, the $(Na_{0.525}K_{0.443}Li_{0.037})(Nb_{0.883}Sb_{0.08}Ta_{0.037})O_3+0.3wt%Bi_2O_3+0.4wt%Fe_2O_3+xwt%CuO$ (x= 0~0.8 wt%)(abbreviated as NKL-NST) lead-free piezoelectric ceramics have been synthesized using the ordinary solid state reaction method. The effects of CuO-doping on the structure and electrical properties of the NKL-NST ceramics were systematically studied. The results show that the ceramics exhibit a pure perovskite structure with orthorhombic phase at room temperature, and secondary phase was found in the ceramics. The 0.4 wt%CuO added ceramics sintered at $950^{\circ}C$ showed the optimum properties of piezoelectric constant($d_{33}$), planar piezoelectric coupling coefficient(kp) and mechanical quality factor(Qm) : $d_{33}=213$[pC/N], kp= 0.43, Qm= 423,respectively.

Surface Acoustic Wave Characteristics of PSS-PZT Cermaics with Cr addition (Cr 첨가에 따른 PSS-PZT 세라믹스의 탄성 표면파 특성)

  • 홍재일;유주현;김준한;강진규;박창엽
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
    • /
    • 1991.10a
    • /
    • pp.68-72
    • /
    • 1991
  • In this study, to improve temperature stability 0.05 Pb(Sn$\_$$\frac{1}{2}$/Sb$\_$$\frac{1}{2}$/O$_3$ - 0.35PbTiO$_3$ - 0.60PbZrO$_3$ + 0.4[wt%]MnO$_2$ piezoelctric oeramics of low dielectric constant and large mechanical quality factor were manufactured with the addition of Cr$_2$O$_3$by Hot Press method. And the SAW delay line was fabricated and the propagation characteristics of SAW was investigated, and the SAW filter was fabricated on C4 added by 0.2[wt%] Cr$_2$O$_3$and its frequency characteristics was investigated. The specimen, whose propagation characteristics of surface acoustic wave were the best, was C4 added by 0.2[wt%] Cr$_2$O$_3$, and its electromechanical coupling factor(ks$^2$) was 3.11[%] and its temperature coefficient of the center frequency (C$\_$fo/) was -21.27[ppm/$^{\circ}C$]. The 31[MHz] SAW IF filter of C4 scarcely had diffraction phenomena and its group delay time was 1.4673 ${\pm}$40[ns] in the pass band, and the insertion loss was -24.419[dB] on no impedance matching.

Factors Influencing Protoplast Isolation and Culture in Three Capsicum Species (세 가지 고추속 식물의 원형질체 분리 및 배양에 미치는 요인)

  • 임학태;염옥희;전익조;조미애;양승균
    • Korean Journal of Plant Tissue Culture
    • /
    • v.28 no.3
    • /
    • pp.141-146
    • /
    • 2001
  • Protoplasts were isolated from cotyledons, hypocotyls, and mesophyll tissues of three species of Capsicum species (C. anuumm, C. bacatuum, and C. chacoense). Combination of Cellulysin (1%) and Macero-zyme (0.25%) in 0.65 M sorbitol was found to be the most effective for the digestion of cell wall, regardless of the Capsicum species. Antioxidant MES (2-[N-Morpholino]ethanesulfonic acid) in the enzyme solution helped protoplasts overcome browning. After 5 days of initial culture, Cell division occurred in modified K8p medium containing 1~5 mg/L zeatin, 0.5 mg/L IAA, 0.1~0.5 mg/L TDZ, and 1 mg/L 2,4-D under continuous dark condition at $25^{\circ}C$. Semi-solid agarose culture method was more effective than liquid culture, and it also protected the cells from browning caused by polyphenolic compound released during protoplast culture. A total of 4000 calli were obtained from protoplast culture of different capsicum species. All of these calli were transferred to the 100 combinations of regeneration media using various plant growth regulators; TDZ, IAA, 2ip, BAP, NAA, and zeatin. These calli derived from protoplast of three species of capsicum were, however, not differentiated into shoots.

  • PDF

Solid Phase Synthesis of N-(3-hydroxysulfonyl)-L-homoserine Lactone Derivatives and their Inhibitory Effects on Quorum Sensing Regulation in Vibrio harveyi (고체상 합성법에 의해 합성된 N-(3-hydroxysulfonyl)-L-homoserine Lactone 유사체들의 Vibrio harveyi 쿼럼 센싱에 대한 저해 효과)

  • Kim, Cheol-Jin;Park, Hyung-Yeon;Kim, Jae-Eun;Park, Hee-Jin;Lee, Bon-Su;Choi, Yu-Sang;Lee, Joon-Hee;Yoon, Je-Yong
    • Microbiology and Biotechnology Letters
    • /
    • v.37 no.3
    • /
    • pp.248-257
    • /
    • 2009
  • The inhibitors against Vibrio harveyi quorum sensing (QS) signaling were developed by modifying the molecular structure of the major signal, N-3-hydroxybutanoyl-L-homoserine lactone (3-OH-$C_4$-HSL). A series of structural derivatives, N-(3-hydroxysulfonyl)-L-homoserine lactones (HSHLs) were synthesized by the solid-phase organic synthesis method. The in vivo QS inhibition by these compounds was measured by a bioassay system using the V. harveyi bioluminescence, and all showed significant inhibitory effects. To analyze the interaction between these compounds and LuxN, a 3-OH-$C_4$-HSL receptor protein of V. harveyi, we tentatively determined the putative signal binding domain of LuxN based on the sequence homology with other acyl-HSL binding proteins, and predicted the partial 3-D structure of the putative signal binding domain of LuxN by using ORCHESTRA program, and further estimated the binding poses and energies (docking scores) of 3-OH-$C_4$-HSL and HSHLs within the domain. In comparison of the result from this modeling study with that of in vivo bioassay, we suggest that the in silica interpretation of the interaction between ligands and their receptor proteins can be a valuable way to develop better competitive inhibitors, especially in the case that the structural information of the protein is limited.

Vertical Neutron Reflectometer at HANARO (하나로 수직형 중성자 반사율 측정장치)

  • Lee J.S.;Lee C.H.;Hong K.P.;Choi B.H.;Choi Y.H.;Kim Y.J.;Shin K.W.
    • Journal of the Korean Vacuum Society
    • /
    • v.14 no.3
    • /
    • pp.132-137
    • /
    • 2005
  • Neutron reflectometer has been installed at HANARO, research reactor in Korea. It has vertical sample geometry and the wavelength of incident neutron beam is $2.459\;\AA$ Neutron fluxes at monochromator and sample position were $4.5\times10^9\;n/cm^2/sec,\;6.64\times10^6\;n/cm^2/sec4 those were measured by gold wire activation method. Also, some reference thin films such as d-PS, $SiO_2$ were measured and analyzedwith HANARO neutron reflectometer. As result of the work, it was certified that minimum reflectivity and available Q range were $10^{-6},\;and\;0.003\sim0.3\;\AA^{-1}$ respectively.