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Effects of the microbiological properties and pathogenicity of Photobacterium damselae subsp. damselae under different culture conditions (배양 조건이 Photobacterium damselae subsp. damselae의 미생물학적 성상 및 병원성에 미치는 영향)

  • Kwon, Mun-Gyeong;Cho, Byoung-Youl;Park, Myeong-Ae
    • Journal of fish pathology
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    • v.22 no.3
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    • pp.239-251
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    • 2009
  • The effects of microbiological properties and pathogenicity of Photobacterium damselae subsp. damselae were investigated under different culture conditions, temperature, pH, NaCl and iron concentration on culture media. Favorable conditions for bacterial growth were between 15-30${^{\circ}C}$, pH 5-9, 0-4% NaCl concentration and iron contents of over 10 mM, whereas the bacterial growth was inhibited under iron chelator existence. When P. damselae was cultured in iron-limited tryptic soy broth, total protein concentration of extracellular products, cytotoxic ability of ECPs on cell line, bacterial viability in flounder serum, phospholipase and siderophore activities of ECPs were significantly increased. On the other hand, the activities of P. damselae cultured under iron-added conditions were decreased. In this study, the iron-limited conditions were similar to the host in which iron concentration is low. During infection caused by P. damselae, the conditions could be related to the pathogenesis of the pathogen.

Phylogenetic Relationships of 3 Korean Neodiplostomum Species (Digenea: Neodiplostomidae) Based on Partial CO1 Gene

  • Pyo, Kyoung-Ho;Lee, Jo Woon Yi;Lee, Jin-Ju;Park, Yun-Kyu;Shin, Eun-Hee;Chai, Jong-Yil
    • Parasites, Hosts and Diseases
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    • v.52 no.3
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    • pp.325-329
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    • 2014
  • The phylogenetic relationships of the 3 Neodiplostomum spp. (Digenea: Neodiplostomidae) occurring in Korea (N. seoulense, N. leei, and N. boryongense) were analyzed using the partial mitochondrial cytochrome c oxidase subunit 1 (CO1) gene. The adult flukes were recovered from Sprague-Dawley rats (N. seoulense) and newborn chicks (N. leei and N. boryongense) experimentally infected with the neodiplostomula from the grass snake, Rhabdophis tigrinus tigrinus. The genomic DNA was amplified using specific primers, and the sequence of CO1 was obtained. According to the results, the pairwise similarity was 96.1% between N. boryongense and N. seoulense, but was 95.0% between N. boryongense and N. leei and 94.2% between N. leei and N. seoulense. The results demonstrated a closer phylogenetic relationship between N. seoulense and N. boryongense. This high relationship of N. seoulense and N. boryongense may be related to their similar morphologic features including the limited distribution of vitellaria and the presence of a genital cone. N. leei is distinct on the other hand with an extensive distribution of vitellaria and the absence of a genital cone.

Formation of ultra-thin $Ta_{2}O_{5}$ film on thermal silicon nitrides (열적 성장된 실리콘 질화막위에 산화 탄탈륨 초박막의 형성)

  • 이재성;류창명;강신원;이정희;이용현
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.32A no.11
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    • pp.35-43
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    • 1995
  • To obtain high quality of $Ta_{2}O_{5}$ film, two dielectric layers of $Si_{3}N_{4}$ and $Ta_{2}O_{5}$ were subsequently formed on Si wafer. Silicon nitride films were thermally grown in 10 Torr ammonia ambient by R.F induced heating system. The thickness of thermally grown $Si_{3}N_{4}$ film was able to be controlled in the range of tens $\AA$ due to the self-limited growth property. $Ta_{2}O_{5}$ film of 200$\AA$ thickness was then deposited on the as-grown $Si_{3}N_{4}$ film about 25$\AA$ thickness by sputtering method and annealed at $900^{\circ}C$in $O_{2}$ ambient for 1hr. Stoichiometry film was prepared by the annealing in oxygen ambient. Despite the high temperature anneal process, silicon oxide layer was not grown at the interface of the layered films because of the oxidation barrier effect of Si$_{3}$N$_{4}$ film. The fabricated $Ta_{2}O_{5}$/$Si_{3}N_{4}$ film showed low leakage current less than several nA and high dielectric breakdown strength.

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A Zero-latency Cycle Detection Scheme for Enhanced Parallelism in Multiprocessing Systems (다중처리 시스템의 병렬성 증대를 위한 사이클의 비 지연 발견 기법)

  • Kim Ju Gyun
    • Journal of KIISE:Computer Systems and Theory
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    • v.32 no.2
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    • pp.49-54
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    • 2005
  • This Paper Presents a non-blocking deadlock detection scheme with immediate cycle detection in multiprocessing systems. We assume an expedient state and a special case where each type of resource has one unit and each request is limited to one resource unit at a time. Unlike the previous deadlock detection schemes, this new method takes O(1) time for detecting a cycle and O(n+m) time for blocking or handling resource release where n and m are the number of processes and that of resources in the system. The deadlock detection latency is thus minimized and is constant regardless of n and m. However, in a multiprocessing system, the operating system can handle the blocking or release on-the-fly running on a separate processor, thus not interfering with user process execution. To some applications where deadlock is concerned, a predictable and zero-latency deadlock detection scheme could be very useful.

Novel swine-origin H1N1 influenza (신종 H1N1 인플루엔자)

  • Lee, Jina;Lee, Hoan Jong
    • Clinical and Experimental Pediatrics
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    • v.52 no.8
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    • pp.862-868
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    • 2009
  • Since its identification in April 2009, a swine-origin H1N1 influenza A virus (S-OIV) which is a reassortment of gene segments from both North American triple-reassortant and Eurasian swine influenza has been widely spread among humans in unexpected rapidity. To date, each gene segment of the 2009 influenza A (H1N1) outbreak viruses have shown high (99.9%) neucleotide sequence identity. As of July 6, 94,512 people have been infected in 122 countries, of whom 429 have died with an overall case-fatality rate of <0.5%. Most confirmed cases of S-OIV infection have been characterized by self-limited, uncomplicated febrile respiratory illness and 38% of cases have also included vomiting or diarrhea. Standard plus droplet precautions should be adhered to at all times. Tests on S-OIV have indicated that current new H1N1 viruses are sensitive to neuraminidase inhibitors (oseltamivir). However, current less virulent S-OIV may evolve into a pathogenic strain or acquire antiviral resistance, potentially with more severe clinical consequences. Efforts to control these outbreaks would be based on our understanding of novel S-OIV and previous influenza pandemics.

Oxidation Behavior of Ti1-xAlxN Barrier Layer for Memory Devices (메모리소자를 위한 Ti1-xAlxN 방지막의 산화 거동)

  • Park, Sang-Shik
    • Korean Journal of Materials Research
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    • v.12 no.9
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    • pp.718-723
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    • 2002
  • $Ti_{1-x}$ $Al_{ x}$N thin films as barrier layer for memory devices application were deposited by reactive magnetron sputtering. The crystallinity, micro-structure, oxidation resistance and oxidation mechanism of films were investigated as a function of Al content. Lattice parameter and grain size of thin films were decreased with increasing the Al content Oxidation of the film with higher Al content is slow and then, total oxide thickness is thinner than that of lower Al content film. Oxide layer formed on the surface is AlTiNO layer. Oxidation of $Ti_{1-x}$ /$Al_{x}$ N barrier layer is diffusion limited process and thickness of oxide layer with oxidation time increased with a parabolic law. The activation energy of oxygen diffusion, Ea and diffusion coefficient, D of $Ti_{0.74}$ /X$0.74_{0.26}$N film is 2.1eV and $10^{-16}$ ~$10^{-15}$ $\textrm{cm}^2$/s, respectively. $_Ti{1-x}$ /$Al_{x}$ XN barrier layer showed good oxidation resistance.

A Study on the Dielectric Characteristics and Microstructure of $Si_3N_4$ Metal-Insulator-Metal Capacitors ($Si_3N_4$를 이용한 금속-유전체-금속 구조 커패시터의 유전 특성 및 미세구조 연구)

  • 서동우;이승윤;강진영
    • Journal of the Korean Vacuum Society
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    • v.9 no.2
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    • pp.162-166
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    • 2000
  • High quality $Si_3N_4$ metal-insulator-metal (MIM) capacitors were realized by plasma enhanced chemical vapor deposition (PECVD). Titanium nitride (TiN) adapted as a diffusion barrier reduced the interfacial reaction between $Si_3N_4$ dielectric layer and aluminum metal electrode showing neither hillock nor observable precipitate along the interface. The capacitance and the current-voltage characteristics of the MIM capacitors showed that the minimum thickness of $Si_3N_4$ layer should be limited to 500 $\AA$ under the present process, below which most of the capacitors were electrically shorted resulting in the devastation of on-wafer yield. According to the transmission electron microscopy (TEM) on the cross-sectional microstructure of the capacitors, the dielectric breakdown was caused by slit-like voids formed at the interface between TiN and $Si_3N_4$ layers when the thickness of $Si_3N_4$ layer was less than 500 $\AA$. Based on the calculation of thermally-induced residual stress, the formation of voids was understood from the mechanistic point of view.

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A Study on Construction of Aids to Navigation Big Data Based on S-201

  • Kim, Yunjee;Oh, Se-woong;Jeon, Minsu
    • Journal of Navigation and Port Research
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    • v.46 no.5
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    • pp.409-417
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    • 2022
  • The International Association of Lighthouse Authorities (IALA) utilizes a questionnaire to investigate the status of Aids to Navigation (AtoN) around the world. However, results of the IALA questionnaire have limited use because respondent understanding is inconsistent. In addition, there is uncertainty regarding the appropriateness of the questionnaire content. Furthermore, the overall response rate is low. Therefore, the status of AtoN is not clearly understood. AtoN data from around the world are generated hourly. Thus, big data solutions are required to effectively exploit the information. Digitization of analog data is an important component of building big data. Hence, the IALA has developed a Maritime Resource Name (MRN) scheme and an information exchange standard. Here, we used the AtoN information exchange standard and designed an S-201-based big data construction process that could collect and manage global AtoN information. In this study, construction of an IALA AtoN portal was proposed as the core of the construction of the AtoN big data. The process was divided into three stages. IALA AtoN portal is developed by IALA with the goal to provide various meaningful statistical analysis results based on AtoN data while managing AtoN information around the world based on S-201. If an AtoN portal capable of constructing S-201-based big data is developed, then a data collection and storage system that can gather basic S-201 AtoN data from the IALA and global AtoN management agencies could be achieved. Furthermore, insightful statistical analysis of AtoN status worldwide and changes in manufacturing technology will be possible.

Study on Inheritance and Transgressions at Cocoon and Shell Weight Characters between Genetically Sex-limited Silkworm Bombyx mori L. Lines

  • Petkov N.;Petkov Z.;Grekov D.;Arnaudova K.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.11 no.1
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    • pp.75-78
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    • 2005
  • Inheritance of cocoon and shell weight characters in silkworm crosses between sex-limited at larva stage lines, including three (NIG$_{1}$ $\times$ TV$_{3/2}$ ,NIG$_{1}$ $\times$ TBV$_{2/24}$ and NIG$_{1}$ $\times$ B$_{2/6}$ ) from Japanese type and three (NIG$_{2}$ $\times$ B$_{2/6}$ ,TV$_{3/2}$ $\times$ NIG$_{1}$ and NIG$_{2}$ $\times$ TBV$_{2/24}$ ) from Chinese type was studied at Regional Centre for Scientific­Applied Service - Vratza during the period of 2000 ­2002. Inheritance of tested characters in F I was deter­mined through genetic parameters, additiveness (a), dominance (d) and their ratio (d/a). Degree and frequency of transgressions in F$_{2}$ were calculated also. Over dominance (d/a(>) 1) with bigger contribution of the parent with higher value was determined at inheritance tested characters. Transgressions obtained at cocoon and shell weight characters were positive and varied in wide limits. The productivity of parent's lines had defined the variability of genetic parameters for degree and frequency of transgressions. Combination of positive transgressions at cocoon and shell weight characters in F2 hybrid populations from sex-limited at larva stage allow us to select individuals with high productive potential for further use in selection programs.

Comparison of Water Quality According to Seasonal Variation in Mokpo and Wando Costal Areas

  • Kim, Woo-Hang;Lee, Young-Sik
    • Journal of Environmental Science International
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    • v.17 no.3
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    • pp.269-273
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    • 2008
  • The objective of this study was to evaluate the relationship between nutrients and phytoplankton. This study was done by the comparison to two costal areas Mokpo, which inflow fresh water, and Wando. In August, salinity of the sea water decreased by 3.5-4.5%o in Mokpo coastal area, but was not nearly decreased in Wando coastal area. This suggests a lot of fresh water inflow in Mokpo coastal area. DIN and DIP were decreased by water temperature increasing in Wando. However, in Mokpo, DIN and DIP were increased greatly during the summer season. Nitrogen was limited to a 10 NIP ratio especially during the summer season in Wando coastal area while phosphorus in Mokpo coastal area was limited with over 28 N/P ratio in all the seasons. Coefficient of determination$(r^2)$ between DIP and Chl.-a was 0.91 in Mokpo coastal area. On the other hand, Coefficient of determination$(r^2)$ between Chl.-a and DIN, DIP were 0.93 and 0.89, respectively, in Wando coastal area. These results suggest DIP in Mokpo and DIN and DIP in Wando might be limited at the increase of phytoplankton.