• Asian-Australasian Journal of Animal Sciences
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• v.14 no.4
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• pp.479-484
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• 2001

The effect of dietary supplementation of sodium salt of isobutyric acid in low protein (10% CP) wheat straw based diet on nutrient utilization and rumen fermentation was studied in ruminally fistulated male crossbred cattle. The study included a 7 day metabolism and a 3 day rumen fermentation trials. The cattle were distributed into two equal groups of 4 each. The animals of control group were fed a basal diet consisting of wheat straw, concentrate mixture and green maize fodder in 40:40:20 proportion whereas branched chain volatile fatty acid (BCFA) supplemented group received a basal diet + isobutyric acid at 0.75 percent of basal diet. The duration of study was 36 days. The feed intake between experimental groups did not differ significantly and the average total DMI (% BW) was 2.01 and $2.28kg\;day^{-1}$ in control and BCFA supplemented diets. The dietary supplementation of BCFA improved (p<0.05) the DM, OM, NDF and cellulose digestibility by 4.46, 6.63, 10.57 and 11.31 per cent over those fed control diet. The total N retention on BCFA supplementation was improved (p<0.01) due to decreased (p<0.05) urinary N excretion. The concentrations of ruminal total N was 37.07 and $34.77mg\;100ml^{-1}$ in control and BCFA fed groups, respectively. Dietary supplementation BCFA significantly (p<0.01) reduced the ruminal ammonia N concentration as compared to control and the mean values ($mg\;100ml^{-1}$) were 13.18 and 9.42 in control and BCFA fed groups. The total VFA concentration was higher (p<0.01) in BCFA supplemented group (101.14 mM) than the control (93.05 mM). Among the VFAs, the molar proportion of acetate was higher (p<0.01) in BCFA supplemented group (71.07 mM) as compared to control (64.98 mM). However, the concentration of propionate and butyrate remained unchanged. Amino acids composition of bacterial hydrolysates was similar in both the groups. Ruminal outflow rate of liquid digesta was higher (p<0.01) in BCFA fed group ($67.56l\;day^{-1}$) than control ($52.73l\;day^{-1}$). It is concluded that the dietary supplementation of Na-salt of isobutyric acid in low protein diet improved the nutrient utilization and ruminal fermentation characteristics.

• Journal of the Korea Organic Resources Recycling Association
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• v.13 no.4
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• pp.136-147
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• 2005

Leachate from landfill sites contains high organics, chloride and ammonium nitrogen in concentration which might be potentially major pollutants to surface and groundwater environment. Most of landfill leachate treatment plants in Korea consist of biological processes to remove BOD and nitrogen. However, the efficiencies of refractory organics removal, nitrification and denitrification have not met frequently the national effluent regulation of wastewater treatment facility, especially in winter season. Simultaneous removal of organics and nitrogen from leachate is strongly necessitated to meet the national regulation on effluents from leachate treatment facilities. The intermittently aerated biological activated carbon fluidized bed(IABACFB) process was applied to treat real landfill leachates containing refractory organics and high concentration of ammonium nitrogen. The IABACFB reactor consisted of a single bed in which BAC fluidizing and an aerating column. The fluidized bed is intermittently aerated through the blower located at the aerating column. Experiments were performed to evaluate the applicability of Intermittently Aerated BACFB for simultaneous removal of refractory organic carbon and ammonium nitrogen of leachate. Organics and ammonia nitrogen($NH{_4}{^+}-N$)are oxidized during the aerobic stage, and nitrite-nitrate nitrogen($NO{_x}{^-}-N$) are removed to nitrogen gas through denitrification reaction during anoxic state. The IABACFB reactor condition reached a steady state within 40 days since the reactors had been operated. The blowing mode of 60 min.-On/60 min.-OFF is more compatible to remove TOC and ($NH{_4}{^+}-N$) operated. The blowing mode of 60 min.-On/60 min.-OFF is more compatible to remove TOC and ($NH{_4}{^+}-N$) simultaneously than the mode of 30 min.-On/90 min.-OFF. The average removal efficiencies of TOC, the refractory organic carbon, and the average efficiencies of nitrification and denitrification were 90%, 75%, 80%, 95%, respectively.

• Polymer(Korea)
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• v.27 no.5
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• pp.470-476
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• 2003

In order to obtain high refractive plastic materials, 1,2 -ethylenedisulfanylbis(2-mercaptomethyl-1-ethanthiol) (ESTT) was newly prepared in good yield by the reaction of 1,2-ethylenedisulfanylbis(2-bromomethyl-1-ethanthiol) (ESTB) with thiourea followed by hydrolysis using an aqueous ammonia solution and characterized by $^1$H-NMR (-SH at 1.7 ppm), $\^$13/C-NMR(-CH$_2$SH at 28.4 ppm) and FT-IR (-SH at 2540 cm$\^$-1/) spectroscopy, etc. Polythiourethanes (PTU) were obtained from the combinations of ESTT with each of 4,4＇-methylenebis(phenylisocyanate) (MDI), tolyene 2,4-diisocyanate (TDI), isophorone diisocyanate (IPDI), mxylene diisocyanate (XDI), and 1,6-diisocyanatohexane (HMDI) in the presence of dibutyltin dilaurylate as a catalyst, in a casting mold, and characterized by FT-IR (existence of N=C=O) spectroscopy and elemental analyzer (sulfur content). Accordingly, their thermal, mechanical and optical properties were investigated by using DSC, TGA, hardness tester and refractometer: both the melting point on DSC and crystallinity on X -ray diffraction (XRD) for specimens of PTUs were not observed. PTUs with T$\_$g/s above 110 $^{\circ}C$ showed good hardness (Shore D) in the range of 86 to 89. Thermal stabilities of PTUs obtained by using ESTT and each of diisocyanates containing aromatic rings were especially good. Also, the optical transmittances of amorphous PTUs through UV-visible source in the range of 400 to 600 nm were good. PTUs showed refractive indexes above 1.60, and their refractive indexes gradually increased with increase of sulfur contents.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.4
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• pp.414-419
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• 2005

This study was performed to compare the treatment efficiencies of two media, newly developed Bio-rock and conventional gravel, in soil clothing contact oxidation process. The composition of synthetic wastewater were $COD_{Cr}$ $150{\sim}370\;mg/L$, $BOD_5$ $150{\sim}270\;mg/L$, T-N $20{\sim}60\;mg/L$, T-P $5{\sim}25\;mg/L$, pH 7 and 2 mL/L of trace element solution. The experiment using two reactors was comparatively conducted for the flow rate of 40 L/d for 13 months, respectively. Initially Bio-rock reactor was increased to pH 12 due to $Ca(OH)_2$ with hydration of cement, but gravel reactor was dropped to pH 4 due to the degradation of organic material and nitrification. This significant pH variation deteriorated the growth and activity of microorganism. But the high pH of Bio-rock seems favorite to ammonia stripping and precipitation of phosphate. Such pH variation of Bio-rock and gravel reactors were finally stabilized to pH 8 and pH 6, respectively. The removal efficiencies of organic compounds from Bio-rock reactor were 96% of $COD_{Cr}$, 98% of $BOD_5$, 80% of T-N and 85% of T-P which stably coping against variation of influent concentration. But those of gravel reactor were 96% of $COD_{Cr}$, 96% of $BOD_5$, 42% of T-N and 40% of T-P, respectively. The Bio-rock was 2 times higher than T-N and T-P in treatment efficiency. And electron-microscopic examination showed that Bio-rock was more favorable to microbial adherence than gravel. The microbial populations were $5.2{\times}10^6\;CFU/mL$ of Bio-rock reactor compared to $2.6{\times}10^6\;CFU/mL$ in gravel reactor. In result Bio-rock was favor to microbial adherence and high treatment efficiency in spite of variation of influent concentration which had the advantages in saving running time and reducing site requirement.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.8
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• pp.1102-1108
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• 2000

An experiment was conducted as a Latin square design with four rumen fistulated local yellow cattle with a mean live weight of 230 kg. The treatments were: $(CLM_0)$ urea-treated rice straw ad libitum plus 1 kg cassava root meal (basal diet), $(CLM_{500})$ basal diet plus 500 g cassava leaf meal, $(CLM_{1000})$ basal diet plus 1,000 g cassava leaf meal, and $(CLM_{1500})$ basal diet plus 1,500 g cassava leaf meal. The results showed that there were differences in dry matter intake of urea-treated rice straw between treatments (p<0.05). The highest total dry matter intake was observed for treatment $CLM_{1500}$, with 2.62 kg DM/100 kg LWt/day, followed by treatments $CLM_{1000}$, $CLM_{500}$ and $CLM_0$, with 2.42, 2.00 and 1.86 kg DM/100 kg LWt/day, respectively. The ruminal ammonia concentration on treatment $CLM_{1500}$ was greater than on treatments $CLM_{1000}$, $CLM_{500}$ and $CLM_0$. There were non-significant differences in the ruminal pH among the treatments. The in sacco degradability of cassava leaf meal and cassava root meal was high, and on average 75 and 85% respectively of the DM had disappeared after 24 h of incubation. Degradation rate of urea treated rice straw was 64% after 72 h of incubation.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.7
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• pp.967-973
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• 2000

A metabolism trial was conducted with 28 Najdi rams allocated into seven dietary groups to evaluate the effect of dietary inclusion of Salicornia bigelovii Torr biomass on nutrient digestibility, rumina I fluid metabolites and nitrogen and mineral balances. Either the stems (ST) or spikes (SP) of this seawater-irrigated halophyte were incorporated into complete diets at rates of 0, 10, 20 and 30% levels, replacing equal amounts of rhodesgrass hay in a ground mixed control diet. Digestibility of DM, OM, EE, NFE and fecal and urinary nitrogen were not affected by increased level of ST in the diet. As level of ST increased from 0 to 20% in the diets, CP digestibility and nitrogen retention approached their maximum (p<0.01), whereas CF digestibility reached its minimum (p<0.01). On the other hand, except for EE, digestion of all nutrients and nitrogen retention were linearly depressed (p<0.01) as SP increased in the diets from 10 to 30% level. Concentration of ammonia-N, total VFA and pH values in the rumen fluid were lower (p<0.01) with the ST- or SP-fed diets than with the control diet. Increasing level of ST or SP in the diet was associated with an increase (p<0.01) in the proportion of acetate and a decline (p<0.01) in molar percentage of propionate in the ruminal fluid. Sodium absorption increased (p<0.01) with increased ST and SP in the diets up to the 10 and 20% level, respectively, followed by constant absorption values up to the 30% level. When the level of ST in the diet gradually increased to 30%, a concomitant increased (p<0:01) in Ca and P absorption were obvious; whereas, increased level of SP in the diets from 0 to 30% resulted in noticeable (p<0.01) depression in Ca and P apparent absorption.

• Journal of Korean Society on Water Environment
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• v.21 no.5
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• pp.477-483
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• 2005

Anammox (Anaerobic Ammonium Oxidation) bacteria is recently discovered microorganism which can oxidize ammonium to nitrogen gas in the presence of nitrite under anaerobic conditions. The anammox process can save an energy for nitrification and need not require a carbon source for denitrification, however, the start-up periods takes a long time more than several months due to the long doubling time (approximately 11 days). In order to find the effects of seeding microorganisms, hydrazine, and nitrite concentration on the enhancement of the anammox activity, five kinds of microorganisms were selected. Among the several kinds of seeding microorganisms, the granule from acclimated microorganisms treating high concentration of ammonia nitrogen (A-1) and sludge from piggery wastewater treatment plant (A-2) were found to have a high anammox activity. In the case of A-1, the maximum nitrogen conversion rate represented 0.4 mg N/L-hr, and the amount of nitrite utilization was high compared to those of other seeding microorganisms. The A-4 represented a higher nitrogen conversion rate to 0.7 mg N/L-hr although the ammonium concentration in the serum bottle was high as 200 mg/L. Meanwhile, the anaerobic granule from UASB reactor treating distillery wastewater showed a low anammox activity due to the denitrification by the remained carbon sources in the granule. Hydrazine, intermediate product in anammox reaction, enhanced the anammox activity by representing 1.4 times of nitrogen gas was produced in the test bottle than that of control, when 0.4 mM of $N_2H_4$ was added to serum bottle which contains 5 mM of nitrite. The high concentration of nitrite (10 mM) resulted in the decrease of the anammox activity by showing lower production of nitrogen gas compared to that of 5 mM addition of nitrite concentration. As a result of FISH (Florescence In-Situ Hybridization) experiment, the Amx820 probe showed a more than 13% of anammox bacteria in a granule (A-1).

• Korean Journal of Ecology and Environment
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• v.46 no.2
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• pp.175-184
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• 2013

Organic carbon and total nitrogen stable isotope ratios of suspended materials were determined at 3 stations (from Mongtan Bridge to Youngsan river estuary barrage) (n=6, each) from November 2008 to August 2009, in order to understand the origin of particulate organic matter at the Estuary of Youngsan River. Allochthonous organic matter, ammonia-N and silicate were increased after heavy rain (in August). Carbon isotope ratios were significantly different between stations in November and August, and it was possible to determine the origin of organic matter. The heavier nitrogen isotope ratios, as well as higher phosphate concentrations, were found in November than other sampling times. Livestock wastewater and farmland input was likely the main causes of these high values. In addition, YS3 station, the nearest site to estuary barrage, appears to be affected by a substantial amount of livestock wastewater and farmland input, considering that nitrogen isotope ratios were heavier than those at the upper sites. These results suggest that the analysis of stable isotope ratios is a simple but useful tool for the determination of organic matter origin in aquatic environments.

• Korean Journal of Environmental Agriculture
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• v.22 no.4
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• pp.278-283
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• 2003

This study was carried out to examine the nitrogen removal rate of a subsurface-flow treatment wetland system which was constructed on floodplain of the Kwangju River from May to June 2001. Its dimensions were 29m in length, 9m in width and 0.65m in depth. A bottom layer of 45cm in depth was filled with crushed granite with about $15{\sim}30\;mm$ in diameter and a middle layer of 10cm in depth had pea pebbles with about 10 mm in diameter. An upper layer of 5 cm in depth contained course sand. Reeds (Phragmites australis) were transplanted on the surface of the system. They were dug out of natural wetlands and stems were cut at about 40 cm height from their bottom ends. Water of the Kwangju River flowed into it via a pipe by gravity flow and its effluent was funneled back into the river. The height of reed stems was 44.2 cm in July 2001 and 75.3cm in September 2001. The number of stems was increased from $80\;stems/m^2$ in July 2001 to $136\;stems/m^2$ in September 2001. Volume and water quality of inflow and outflow were analyzed from July 2001 through December 2001. Inflow and outflow averaged 40.0 and $39.2\;m^3/day$, respectively. Hydraulic detention time was about 1.5 days. Average nitrogen uptake by reeds was $69.31\;N\;mg/m^2/day$. Removal rate of $NO_3-N$, $NH_3-N$, T-N averaged 195.58, 53.65, and $628.44\;mg/m^2/day$, respectively. Changes of $NO_3-N$ and $NH_3-N$ abatement rates were closely related to those of wetland temperatures. The lower removal rate of nitrogen species compared with that of subsurface-flow wetlands operating in North America could be attributed to the initial stage of the system and inclusion of two cold months into the six-month monitoring period. Increase of standing density of reeds within a few years will develop both root zones suitable for the nitrification of ammonia and surface layer substrates beneficial to the denitrification of nitrates into nitrogen gases, which may lead to increment in the nitrogen retention rate.

• Journal of Practical Agriculture & Fisheries Research
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• v.23 no.2
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• pp.63-70
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• 2021

In order to compare and investigate the growth rates of each of the various soils, the soil mixing ratios were varied to four soils (Pitmos, Pearlite, Masato, General Soil, and Cocopeat). Ten were selected for each soil ratio and the average length and weight were compared. As a result, in the ratio of No. 1 pitmos 6.5: Perlite 2: Masato 1.5, it was measured as 16.36cm, 0.60g. In the ratio of No. 2 pitmos 10, 13.74cm, 0.41g. In the ratio of No. 3 general clay 10, it was measured as 12.43cm, 0.26g. 4 general clay 8, 0.39g. The growth rate of each soil was measured to be superior to that of other soil growth environments in the ratio of pitmos 6.5: pearlite 2: masato 1.5 soil. For ginseng plant analysis, 30 ginseng plants grown in the average length and weight of each soil at a ratio of 6.5: pearlite 2: masato 1.5 and relatively low-result general soil were selected and analyzed. As a result, 1,040ppm of nitrite nitrogen(NO₃-N) was higher in ginseng plants grown in general soil. There was no significant difference in phosphoric acid(P), potassium(K), and magnesium(Mg). Ginseng is characterized by poor growth when it exceeds 300ppm by combining ammonia tae (NH₄-N) and nitrate tae (NO₃-N) nitrogen. In addition, nitric acid produced in a part of this nitrite makes the pH reaction of the soil acidic, and the nitrite remaining in the soil evaporates into gas.