• 한국작물학회:학술대회논문집
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• 한국작물학회 2005년도 춘계학술발표회 요지
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• pp.158-159
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• 2005

• 한국농공학회:학술대회논문집
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• 한국농공학회 2003년도 학술발표논문집
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• pp.619-622
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• 2003

The concentrations of nutrients in precipitation increased slightly from May to June and did not change afterwards. Regarding irrigation water, the nutrient concentrations were high in the early stage of rice growth but decreased during the period of mid-June to mid-July. The concentration of Tot-N in runoff water increased significantly during the period of fertilizer application (basal, tillering, and panicle fertilization) and then decreased. The concentrations of Tot-N in runoff water ranged from 0.4 to 39.8mg/L (average of 5.9mg/L). The concentration of Tot-P in runoff water ranged from 0.0004 to 0.2084mg/L (average of 0.055mg/L). The Tot-P concentrations were high only at the early stage of rice growth after fertilizer application and did not change afterwards.

• Applied Biological Chemistry
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• 제46권2호
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• pp.134-139
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• 2003

본 연구는 3요소$(N-P_2O_5,-K_2O)$처리가 산국(Chrysanthemum boreale M.)의 수량과 유효성분에 미치는 효과를 조사하여 효과적인 재배시스템을 개발하고자하였다. 처리구는 무처리, NPK(250-160-160), NP(250-160-0), NK(250-0-160), PK(0-160-160 kg/ha)의 5처리로 설정하여 산국을 재배한 결과, 건물수량은 NPK처리구가 PK처리구에 비하여 4.0배의 증수가 있었으며, 다른 처리구보다 무기이온의 흡수율, 생육 및 수량에서 NPK처리구가 가장 우수하였다. 식용으로 많이 쓰이는 꽃의 주요 아미노산은 proline, glutamic acid과 aspartic acid이였고, 다른 처리구들에 비하여 NPK처리구가 높은 함량을 보였다. 혈압강하효과가 우수한 cumambrin A는 PK처리구에 비하여 NPK처리구가 6.2배의 증수효과가 있었다. NK처리구는 NP처리구보다 cumambrin A함량이 우수하였고, 식물체 중의 칼리 함량과 cumambrin A의 함량과는 상관관계가 인정되었으나, 이에 대한 생리적 대사과정은 아직까지 밝혀져 있지 않다. 따라서 산국의 재배시 cumambrin A의 함량을 높이기 위해서는 인산의 시용보다 칼리의 시용이 더 요구되며, 야외포장에서의 인산과 칼리의 적정 시비량의 설정이 필요하다고 판단된다.

• 한국수산과학회지
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• 제29권4호
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• pp.497-502
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• 1996

1990년 3월, 경상북도 영덕군 강구면에 위치한 오십천과 경상남도 밀양시 밀양강에서 채집하여 인공수정한 난을 실험실로 운반하여 난발생과정과 부화자어의 성장에 따른 형태변화를 관찰한 결과는 다음과 같다. 1. 빙어의 수정난은 구형의 침성부착난으로 무색 투명하며, 난경은 $0.85\~1.05mm$ (평균 0.97mm, n=50)이다. 2. 부화에 소요된 시간은 평균 $16.5^{\circ}C$에서 약 170시간만에 부화한다. 3. 부화직후의 자어는 전장 $3.85\~4.25mm$ (평균 4.05 mm, n=10)로, 아직 입과 항문이 열려 있지 않고, 눈에 색소포가 착색되어 있으며, 근절수는 $52\~54$개이다. 4. 부화 $4\~5$일째의 자어는 전장이 $5.20\~5.65mm$ (평균 5.37mm, n=10)로 난황은 거의 흡수되고, 눈의 색소는 더욱 짙어진다. 5. 부화 20일째의 후기자어는 전장이 $8.20\~8.55\;mm$(평균 8.38mm, n=10)로 척색말단이 $45^{\circ}$ 각도로 위로 굽어진다. 6. 부화 25일째의 후기자어는 전장이 $9.15\~9.75mm$ (평균 9.63mm, n=10)로 등지느러미와 뒷지느러미에 각각 융기하기 시작하여 원기가 나타난다.

• Clinical and Experimental Reproductive Medicine
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• 제25권2호
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• pp.109-113
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• 1998

Objective: To ananlyze the direct effect of nitric oxide (NO), generated from sodium prusside (SNP) on the embryo developments in reproductive process. Design: Ova from mouse were treated to allow fertilization in in vitro culture. And the samples of fertilized ova were alloted into five alliqutos. Each alliquot was cultured in media treated with either concentration at 0 (n=92), $25{\mu}M$ (n=84), $50{\mu}M$ (n=80), $100{\mu}M$ (n=77), $500{\mu}M$ (n=54) of SNP. Main Outcome Measure: Rates of embryonal cell cleavages, viability and cell morphology were assessed during in vitro fertilization and culture. Results: As analyse the cell cleavage at 24 hours after in vitro culture of fertilised egg in variuos NO concentration, all of egg cells of each alliquot were developed to $2\sim4$ cell stage. But the alliquot of egg cells treated with $50{\mu}M$, which were totally degenerated. And also all embryonal cells of each alliquot were developed to 8 cell stage and morula stage on culture continuosly. And the embryonal cells of each alliquot were analysed at 24 and 48 hours following the in vitro culture. The rates of cell fragmentation and fusion were $4.2{\pm}3.4%$ in control group which is not treated with NO, while experimental groups was high, as rated $23.4{\pm}6.2%$ in $25{\mu}M$, $28.2{\pm}5.7%$ in $50{\mu}M$ and $32.1{\pm}6.4%$ in $100{\mu}M$ concentration of NO. Accordingly the rate of abnormal morphology of embryonal cell in control was lower significantly than that in each alliquot of experimental groups (p<0.05). And the degenerated rates of embryonal cells were 0% in control, $17.8{\pm}6.7%$ in $25{\mu}M$, $23.6{\pm}4.7%$ in $50{\mu}M$ and $26.8{\pm}11.2%$ in $100{\mu}M$ at 8 cells and morula on culture of 48 and 72 hours. On the examination of embryonal cells developed to blastocyst through in vitro culture, the rates of degenerated cells were $16.8{\pm}7.2%$ in control, $37.5{\pm}6.2%$ in $25{\mu}M$, $73.4{\pm}4.6%$ in $50{\mu}M$, 100% in $100{\mu}M$. Conclusion: This results suggeted that the NO in any concentrations is harmful on embryos in view of morphology as well as viability of cell, and the toxicity of NO on embryo is stronger at condition in higher concentration of NO.

• 한국초지조사료학회지
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• 제40권2호
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• pp.117-122
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• 2020

국내에서 말 사육 두수가 가장 많고 화산회토양으로 이루어져 있는 제주도에서 마분 퇴비의 시용수준이 이탈리안 라이그라스의 생산성과 수질 환경에 미치는 영향을 알아보기 위해 본 연구를 수행하였다. 사료작물은 이탈리안 라이그라스(코윈어리)를 공시하였고 용탈수 성분 분석을 위해서 간이 Lysimeter를 제작하였다. 퇴비 시용 수준에 따라 6처리하였고, 이탈리안 라이그라스의 수량 등 생산성을 조사하였다. 용탈수는 7회 채취하여 NO₃-N, PO₄-P, Cr, Cu, Zn을 분석하였다. 마분 퇴비 시용 수준에 따른 이탈리안 라이그라스의 건물 수량은 화학비료구가 유의적으로 가장 높게 나타났고(11,965±564 kg/ha), 마분 퇴비 150%, 혼용구가 그 다음으로 유의적으로 높게 나타났으며(9,043±681 kg/ha, 8,825±611 kg/ha), 마분퇴비 100%, 50%가 그 다음으로 나타났다(4,293±804 kg/ha, 2,986±739 kg/ha)(p<0.05). 용탈수의 분석항목 중 질산태 질소(NO₃-N)의 경우 3차 분석까지는 전체적으로 증가하다가 감소하는 경향을 보였고 마지막 6, 7차 분석 시에는 검출되지 않았다(Fig. 2.). NO₃-N는 처리구 간에 유의적인 차이는 없었으나 수치적으로 마분 퇴비 150% 시용구에서 NO₃-N가 가장 많은 경향이 나타났고, 화학비료구와 혼용구는 수치적으로 마분 퇴비를 시용했을 때보다 적거나 비슷한 수준을 나타났다. NO₃-N를 제외한 나머지 PO₄-P, Cr, Cu, Zn은 전 기간 거의 검출되지 않았다. 본 연구 결과를 종합했을 때 마분 퇴비를 시용할 때는 기비로 마분 퇴비를 질소 기준 50%수준 시비하고 이듬해 봄 추비로 화학비료를 나머지 50% 수준으로 시비하면 수량이 크게 떨어지지 않으면서 수질 오염에도 상대적으로 적은 영향을 줄 수 있을 것으로 사료된다.

• 한국어류학회지
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• 제26권1호
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• pp.34-41
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• 2014

The present study describes the spawning ecology and early morphological development of Hemitripterus villosus. The natural spawning ground consisted of bedrock and pebbles was the intertidal coast at Taean (Chungnam) and its depth was about 5~10 m. Spawning period was mainly from the end of October to December, when the water temperature and salinity were $6.0{\sim}15.8^{\circ}C$ and mean 32.0‰, respectively. There were no difference of the body shape and color between female and male of Hemitripterus villosus, however its reproductive organs showed clear differences. The male had tube shaped genital papilla, which was connected with testis, and the female had seminal recepacle, which was the lower part of oviduct connected with ovary. Genital papilla of male came out of its body at spawning period and then male copulated. After copulation, female stored the sperm in its seminal recepacle and fertilized when it spawned. Fertilized eggs were reached 8 cells stage after fertilization at rearing water temperature $8.2{\sim}14.9^{\circ}C$. At 29 hours after fertilization, it reached morula stage, and at 146 hours after fertilization, its embryo was clearly formated. Hatching was begun from 1,488 hours (62 days) after fertilization with $8.2{\sim}14.9^{\circ}C$ water temperature. The newly hatched larvae were 12.99~15.46mm(mean $14.16{\pm}0.65$ mm) in TL (Total Length), and its mouth and anus were open. At 7 days after hatching, its yolk sac was completely absorbed and the myotomes were 15+25=40, measuring 15.23~15.54mm(mean $15.39{\pm}0.22$ mm, n=5) in TL. At 75~80 days after hatching, it was measured mean $30.06{\pm}0.76$ mm in TL, and it had reached the juvenile stage with the complete set of fin rays.

• Asian-Australasian Journal of Animal Sciences
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• 제17권2호
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• pp.164-167
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• 2004

This study was carried out to investigate in vitro fertilization and development of in vitro matured pig oocytes inseminated with the Duroc boar sperm by different sperm washing media after thawing of the 5 ml frozen straws. Immature follicular oocytes (30-40) were transferred into each well of a Nunc 4-well multidish containing $500{\mu}l$ mTCM199 maturation medium. The sperm rich portion of ejaculates was collected into a 250 ml insulated vacuum bottle and gradually cooled 22 to $24^{\circ}C$ over a 2 h period. Semen was centrifuged at 800 g for 10 min and the seminal plasma discarded. Sperm were esuspended in a lactose-egg yolk and N-acetyl-Dglucosamine (LEN) diluent to contain $1{\times}10^{9}$ sperm/ml and cooled to $5^{\circ}C$ over a 2 h period. Immediately before freezing, semen was rediluted with an equal volume of LEN+4% glycerol and packed into 5 ml straws. After thawing of the 5 ml straw, the 5 ml semen was diluted with 20 ml Beltsville thawing solution (BTS) at room temperature. Oocytes were inseminated with untreated (unwashed and nonpreincubated) or treated sperm (washed two times in BTS, mTLP-PVA and mTBM media, respectively and nonpreincubated) with $2{\times}10^{7}$ sperm concentration. Oocytes were coincubated for 6 h in $500{\mu}l$ mTBM fertilization. At 6 h after IVF, oocytes were transferred into $500{\mu}l$ NCSU-23 culture medium for further culture of 6 h. Sperm penetration, polyspermy and male pronuclear formation of oocytes at 12 h after IVF and developmental ability of oocytes at 48 h after IVF were evaluated. Sperm penetration rate, male pronuclear formation and rate of cleaved embryos were higher in the BTS, mTLP-PVA and mTBM treatments than the unwashed treatment (p<0.05). The rate of blastocysts from the cleaved oocytes (2-4 cell stage) were higher in the mTLP-PVA treatment than in the unwashed, BTS and mTBM treatments. In conclusion, we recommend the washing of frozen-thawed sperm with mTLP-PVA medium before in vitro fertilization of oocytes in mTBM medium.

• Clinical and Experimental Reproductive Medicine
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• 제26권2호
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• pp.193-201
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• 1999

본 연구에서는 소 난자 세포질내 원형정자 및 원형정자 핵을 미세주입 후 수정과정과 체외 배 발달을 조사하였다. 원형정자 주입 3시간 전에 인위적 자극을 주고 원형정자를 주입했을 때 난자들의 수정률이 주입 직후에 자극을 준 것들과 자극을 주지 않은 것들 보다 높았다. 원형정자 주입과 원형 정자핵을 주입한 후 전핵 형성률과 전핵 이동률을 조사하였으나 유의차를 발견할 수 없었다. 전핵의 형성과 이동중에 미세소관의 움직임을 간접형광면역법 및 공촛점 현미경을 사용하여 조사해 본 결과, 난활성 직후 난자의 표층에서 미세소관이 발생해서 이것에 의해 웅성 및 자성전핵이 난자 중심부로 이전됨을 볼 수 있었다. 수정 후 원형정자의 세포질내 Mitochondria의 분포상태를 알아보기 위해 MitoTracker에 노출시킨 원형정자를 주입한 후 형광현미경에서 관찰해 보았다. 그 결과 원형정자의 Mitochondria가 주입 후 4-cell 이 전까지 는 관찰되었으나 그 이 후에는 발견 할 수 없었다. 원형정자를 미세주입한 후 소 난자를 체외에서 배양했을 때 2.5%의 난자가 배반포로 발달하였다. 이상의 결과는 체외 배양한 소 난자 내에 원형정자 혹은 원형정자 핵을 미세 주입하여 정상적인 수정과 배발달이 가능한 것을 보여 주는 것이다.

• 한국환경농학회지
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• 제41권4호
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• pp.230-235
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• 2022

BACKGROUND: Ammonia gas emitted from nitrogen fertilizers applied in agricultural land is an environmental pollutant that catalyzes the formation of fine particulate matter (PM2.5). A significant portion (12-18%) of nitrogen fertilizer input for crop cultivation is emitted to the atmosphere as ammonia gas, a loss form of nitrogen fertilizer in agricultural land. The widely practiced method for fertilizer use in agricultural fields involves spraying the fertilizers on the surface of farmlands and mixing those with the soils through such means as rotary work. To test the potential reduction of ammonia emission by nitrogen fertilizers from the soil surface, we have added N, P, and K at 2 g each to the glass greenhouse soil, and the ammonia emission was analyzed. METHODS AND RESULTS: The treatment consisted of non-fertilization, surface spray (conventional fertilization), and soil depth spray at 10, 15, 20, 25, and 30 cm. Ammonia was collected using a self-manufactured vertical wind tunnel chamber, and it was quantified by the indophenol-blue method. As a result of analyzing ammonia emission after fertilizer treatments by soil depth, ammonia was emitted by the surface spray treatment immediately after spraying the fertilizer in the paddy soil, with no ammonia emission occurring at a soil depth of 10 cm to 30 cm. In the upland soil, ammonia was emitted by the surface spray treatment after 2 days of treatment, and there was no ammonia emission at a soil depth of 15 cm to 30 cm. Lettuce and Chinese cabbage treated with fertilizer at depths of 20 cm and 30 cm showed increases of fresh weight and nutrient and potassium contents. CONCLUSION(S): In conclusion, rather than the current fertilization method of spraying and mixing the fertilizers on the soil surface, deep placement of the nitrogen fertilizer in the soil at 10 cm or more in paddy fields and 15 cm or more in upland fields was considered as a better fertilization method to reduce ammonia emission.