• Journal of Korean Society of Forest Science
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• v.90 no.3
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• pp.306-313
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• 2001

The objectives of this study were to understand the physiological responses and resistance of red pine trees to ozone exposure in relation to nitrogen and phosphorus fertilization. Potted one-year-old seedlings of Pinus densiflora S. et Z. were exposed in an open-top chamber(OTC) to ozone at concentration of 0.12ppm for 3 hours daily for eight weeks with or without N and P fertilization alone or in combination. The OTC had dimensions of 2.0m in height and 2.5m in diameter, and the air in a control chamber was filtered with activated charcoal to maintain the ozone concentration below 0.02ppm. After eight weeks of ozone exposure, none of the seedlings showed any symptoms of visible injury on leaves. The seedlings fertilized with N and P in a control chamber showed 22 to 95% increase in total dry weight, and similar fertilizer effect was also noticed in an ozone chamber. Ozone treatment did not decrease the total dry weight, but increased shoot/root ratio by 14.5%. Ozone treatment increased sucrose content in the leaves by 23%, but decreased sucrose content in roots by 20% regardless of N or P application. Starch content in the leaves was not affected by either ozone or fertilizer. However, starch content in the roots was decreased by 41% by ozone treatment. Chlorophyll content in the leaves was increased by 70% by N application, but was not affected by ozone treatment. Nitrogen and P fertilization stimulated net photosynthesis by 80% in a control chamber, but stimulatory effect of N and P on net photosynthesis was 22.3% less in an ozone chamber. Net photosynthesis of the seedlings with no fertilization was not affected by ozone treatment. Based on the observed interactions between N, P, and ozone, it was concluded that the stimulatory effect of fertilization on growth of Pinus densiflora would be decreased by ozone treatment, but fertilization would increase resistance to ozone by re-allocation of increased carbohydrates.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.3
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• pp.189-194
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• 1991

This experiment was carried out to investigate the effect of pure mineral nitrogen fertilization on dry matter yield and to determine the amounts of advisable mineral nitrogen according to difference of annual precipitations in permanent grassland. The results obtained were as follows: 1. Only PK-fertilizing without additional mineral nitrogen application produced 6. 9 tonlha of annual dry matter yields on average in relatively wet years that was higher than in rel. dry and normal years under 3-cut system by 0. 7 and 0.6 ton DMIha, respectively. 2. Due to the lesser rainfall the nitrogen-efficiency was substantially higher with heavy dressing of nitrogen fertilizer. The absolute differences in yield between rel. dry and wet years were 0.4 and 0.7 ton DMIhalyear respectively when dressed with 90 and 120 kgN/ha/cut at 4-, 5- and 6-cut systems, whereas at 3-cut system differences around 1.3 and 1.1 ton Dhllhalyear respectively were recorded. 3. In rel. dry years the most efficient N-dressing rates per ha and year tended to be slightly higher than in rel. wet and normal years. Particularly at 5-cut system 4-7 kg/ha/cut of more nitrogen fertilization was required in order to obtain the highest overyields. 4. The N-dressing rates needed to maintain a nitrogen-efficiency of 8 to 16 kg DM/kg N tended to be stronger particularly at high cut system, and also in rel. dry years higher dressing rates were required that in rel. wet and normal years.

• Journal of Animal Science and Technology
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• v.57 no.4
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• pp.16.1-16.7
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• 2015

A well-characterized sperm specific protein of the Member of immunoglobulin superfamily, IZUMO1, has crucial role in fertilization by mediating sperm binding to the egg plasma membrane in the mouse. However little is known about IZUMO1 in bovine. Here, we describe the molecular cloning and expression analysis of bovine IZUMO1 (bIZUMO1). RT-PCR and Western blot analysis of the bovine tissues indicated that bIZUMO1 was specifically expressed in the testis and sperm, Furthermore, the result of our biotinylation assay from ejaculated bovine sperm strongly suggest the assumption that bIZUMO1 is localized on the cell surface. These data imply the potential role of bovine IZUMO1 in mammalian fertilization.

• Journal of Korean Society of Forest Science
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• v.86 no.3
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• pp.311-318
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• 1997

Chemical properties of soil(N, P, K, Ca, Na, Mg, CEC, and pH) were studied after annual additions of $NH_4NO_3$(336kg/ha N), treble superphosphate(112kg/ha P), and KCl(224kg/ha K) fertilizers in a willow(Salix spp.) bioenergy plantation. Soil samples were collected from November through December 1992 from previously established the fertilized and non-fertilized willow plantation at Tully, New York, U.S.A. in 1987. Total fertilizer additions from 1987 through 1991 were 1,680kg/ha N and 560kg/ha P and 1.120kg/ha K. Fertilization with N, P, and K resulted in no difference in total soil N content between the fertilized and non-fertilized plots, increased soil P and K, decreased base cations ($Ca^{2+}$ and $Mg^{2+}$) and soil pH, and increased soil pH with soil depth. Strong positive correlations of soil carbon to soil N, Ca, Mg, and CEC were noted. Soil C/N ratio in the study plots ranged from 9.6 to 11.2 for all treatment combinations. Significant differences in soil P, K, Ca, and pH between the fertilized and non-fertilized plots indicate that fertilization had changed chemical properties of soil in this fertilizer trial.

• Korean Journal of Agricultural Science
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• v.11 no.2
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• pp.207-217
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• 1984

Seasonal growth performances or root collar diameter and seedling height of Quercus acutissima. Q. mongolica and Q. variabilis were measured at regular intervals of 10 or 15 days after the treatments of some combinations of soil water regime ${\times}$ fertilization. The treatments of soil water regime and fertilization Influenced on the growth performances of seedlings differently with one another in course of time lapse. The growth performances revealed highly significant differences between soil water regimes, between fertilizations and between their interactions after unlike time lapses by species. The effects of soil water regime were exhibited in retard compared with those of fertilization, and to be different outstandingly in the treatments of N or N+P+K fertilization. The limit of soil water potential influencing critically on the growth performances might be estimated to be in -3~-6 bar in all the species. The growth responses were significantly different between N or N+P+K treatment and P or K treatment or control in all the species, and the treatment effects represented more great differences in moist soil water regime than in dry soil water regime. The interactions of soil water regime ${\times}$ fertilization were revealed s lowly with time lapse in all the treatments. The statistical differences of growth responses of root collar diameter according to the treatments were observed in earlier stage compared with those of seedling height. By comparison of the growth responses of the species studied, Q. variabilis and Q. mongolica seemed to be more resistant to moisture stress than Q. acutissima.

• Journal of Embryo Transfer
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• v.17 no.2
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• pp.117-121
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• 2002

This study was carried out to investigate the in vitro fertilization rate of canine immature oocytes cryopreserved by vitrification freezing. The vitrification solutions of EPS and EDS were consisted of 40％ ethylene glycol 18％ Ficoll and 0.3M sucrose, and 20％ ethylene glycol, 16.5％ DMSO and 0.5M sucrose in TCM-199 medium supplemented with 10％ FCS, respectively. The oocytes were exposed The developmental rate of in vitro cultured oocytes recovered from ovaries collected at different stages of the reproductive cycle were 3.8％, 10.7％, 46％, respectively. to EFS or EDS at $25^{\circ}C$ and loaded into straw fer 30 sec. The straws was slowly immersed into L$N_2$. Fertilization and survival rate was defined as development rate on in vitro culture or FDA-test. 1. The fertilization rate after vitrification freezing of immature oocytes at 1, 6, 12 and 24 hrs after collection from ovaries was very low(5.3％～31.4％) than the unfrozen oocyte(60.0％). And the fertilization rate after vitrification freezing of immature oocytes was very higher than that of mature oocytes. 2. The survival rate after vitrification freezing of immature oocytes at 1, 6, 12 and 24 hrs after collection from ovaries was 55.0％, 40.0％, 28.6％ and 17.1％, respectively. And the survival rate after vitrification freezing of immature oocytes was slightly higher than that of mature oocytes.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.1
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• pp.47-52
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• 1996

To investigate the effects of phosphate($P_2O_5$) fertilization levels on the dry matter(DM) yield, nutrition yield, and $P_2O_5$ efficiency of forages in mixed sward of alfalfa-grasses, a field experiment arranged by randomized complete block design with five treatments(0, 50, 100, 150 and 200kg $P_2O_5$/ha) was conducted at Livestock Experiment Station in Suwon, September, 1990 to Feb~ary, 1993. During two years, average DM yields of forages increased as $P_2O_5$ fertilization level was increased, but no significant difference was found between DM 10,754kg of $P_2O_5$ 150kg/ha and DM 10,845kg of $P_2O_5$ 200kg/ha. Cmde protein and energy productivity of forages increased as $P_2O_5$ fertilization level was increased, and the increasing degree was highest between $P_2O_5$ l00kg and 150kg/ha feriilization. With increasing $P_2O_5$ fertilization, mineral contents tended to increase in P, K and WCa+ Mg equivalent ratio, but not to be regular in the other elements. Efficiency of $P_2O_5$ was highest at $P_2O_5$ 150kg/ha fertilization, which produced DM 12.8kg. net energy lactation 75.4MJ. starch equivalent 6.lkStE, and total digestible nutrients 8.6kg per Ikg $P_2O_5$.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.2
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• pp.69-75
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• 1994

This experiment was canied out to study the effect of cutting frequencies (3, 4 and 5 cuts per year) and mineral nitrogen fertilization on dry matter yields, in order to estimate optimum level of fertilizing mineral nitrogen in orchardgrass(Dacry1is glomerafa L.). The results are as follows: 1. Dry matter yields were the highest in the 1st cut at 3 cutting frequency and in the 2nd cut at 4 and 5 cutting frequencies, and they shared 49.7, 37.0 and 37.0% of annual dry matter yield respectively. 2. When only phosphorus and potassium were applied, annual dry matter yields were between 9.4 and 11.5 tons per ha and the highest yield was observed at 3 cutting frequency. 3. Dry matter yield in relation to fertilization of mineral nitrogen was highly increased when more nitrogen was applied as 360 kg per ha compared to 240 kg per ha at 3 cutting frequency. At 4 and 5 cutting frequencies, increases in dry matter yield, to large extent, were showed at 120 and 150 kg of nitrogen per ha(30 kg Nlcutlha) compared to no application of nitrogen. The efficiencies of mineral nitrogen fertilization (kg DM/kg N) were 28.0, 22.7 and 20.6 kg dry matter yields per nitrogen(kg) respectively). 4. At 4 and 5 cutting frequencies, dry matter yields were reduced when 360 kg and 300 kg of mineral nitrogen per ha(90 kg and 60 kg Nlcutlha) were applied respectively. 5. Particularly at 4 and 5 cutting frequencies of this study, maximum marginal yields reached at 129.9 kg and 148.0 kg of fertilizing mineral nitrogen per ha, and economical borden of mineral nitrogen fertilization were between 222.4 and 250.0 kg and between 244.8 and 276.8 kg respectively. At the same cutting frequencies, the highest dry matter yields were obtained at 365.4 and 433.8 kg of fertilizing mineral nitrogen respectively.

• Proceedings of the Korean Society of Crop Science Conference
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• 1987.06a
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• pp.62-63
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• 1987

• Horticultural Science & Technology
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• v.35 no.4
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• pp.431-438
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• 2017

This study investigated the effect of pre-planting nitrogen (N) fertilization levels added to a soilless root medium on the growth of 'Dotaerang Dia' tomato seedlings. The N levels were varied for a total of 7 treatments: 0, 100, 250, 500, 750, 1,000, or $1,500mg{\cdot}L^{-1}$. The pH of the root media gradually rose in all treatments as the seedlings grew; however, the differences in the pH were not significant among the treatments. The electrical conductivity (EC) of the root media was significantly different among the treatments from sowing to week three, then drastically decreased after week four, which diminished the differences in the EC among the treatments. At week six, plant height, leaf length, leaf width, number of leaves, and fresh and dry weights of the shoot were highest for the treatment with $500mg{\cdot}L^{-1}N$. In contrast, the treatment with $1,500mg{\cdot}L^{-1}N$ had the lowest results for all growth measurements. The fresh weight was 67% heavier in the $500mg{\cdot}L^{-1}N$ treatment compared to the $1,500mg{\cdot}L^{-1}N$ treatment. The total N content in the tissues was lowest in the treatment with $0mg{\cdot}L^{-1}N$ and highest in the treatment with $1,000mg{\cdot}L^{-1}N$. The contents of calcium (Ca), magnesium (Mg), and metal micronutrients in the tissues were highest in the $250mg{\cdot}L^{-1}N$ treatment. A previous study demonstrated that adjusting the fertilization level to promote growth to over 90% of the maximum growth is a good strategy for lowering production costs and preventing damage due to excessive fertilizer absorption by crops. Our results indicated that the optimal pre-planting N fertilization level for tomato plug seedlings should be lower than $500mg{\cdot}L^{-1}$ and the optimum tissue N contents should be around 3.21% to 4.60%.