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Studies on In Vitro Fertilization after Vitrification Freezing of Immatured Canine Oocytes  

박상훈 (충남대학교 수의과대학)
박종민 (충남대학교 수의과대학)
김상근 (충남대학교 수의과대학)
Publication Information
Journal of Embryo Transfer / v.17, no.2, 2002 , pp. 117-121 More about this Journal
Abstract
This study was carried out to investigate the in vitro fertilization rate of canine immature oocytes cryopreserved by vitrification freezing. The vitrification solutions of EPS and EDS were consisted of 40％ ethylene glycol 18％ Ficoll and 0.3M sucrose, and 20％ ethylene glycol, 16.5％ DMSO and 0.5M sucrose in TCM-199 medium supplemented with 10％ FCS, respectively. The oocytes were exposed The developmental rate of in vitro cultured oocytes recovered from ovaries collected at different stages of the reproductive cycle were 3.8％, 10.7％, 46％, respectively. to EFS or EDS at $25^{\circ}C$ and loaded into straw fer 30 sec. The straws was slowly immersed into L$N_2$. Fertilization and survival rate was defined as development rate on in vitro culture or FDA-test. 1. The fertilization rate after vitrification freezing of immature oocytes at 1, 6, 12 and 24 hrs after collection from ovaries was very low(5.3％～31.4％) than the unfrozen oocyte(60.0％). And the fertilization rate after vitrification freezing of immature oocytes was very higher than that of mature oocytes. 2. The survival rate after vitrification freezing of immature oocytes at 1, 6, 12 and 24 hrs after collection from ovaries was 55.0％, 40.0％, 28.6％ and 17.1％, respectively. And the survival rate after vitrification freezing of immature oocytes was slightly higher than that of mature oocytes.
Keywords
dogs; immature oocytes; vitrification freezing; fertilization; survival rate;
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1 Kim SK, Park SH and Suk HB. 2000. Studies on the effects of cryoprotectant kinds and cell stages on the viability of bovine embryos cryopreserved by vitrification. Korean J. Anim. Rerpod., 24(3):225-230
2 Candy CJ, Wood M, Whittingham DG, Merriman JA and Choudhury N. 1994. Cryopreservation of immature mouse oocytes. Hum. Rerpod., 9:1738-1742   DOI
3 Schilling E, Niemann H and Schmidt D. 1982. Evaluation of fresh and frozen cattle embryos by fluorescence microscopy. Cryobiology, 15:245-248   DOI   PUBMED   ScienceOn
4 Schroeder AC, Champlin AK, Mobraten LE and Eppig JJ. 1990. Developmental capacity of mouse oocytes cryopreserved before and after maturation in vitro. J. Reprod. Fert., 89:43-50   DOI
5 Vajta G, Holm P, Kuwayama M, Booth PJ, Jacobsen, A., Greve, T. and Callesen, H. 1998a. Open pulled straw vitrification; a new way to reduce cryoinjures of bovine ova and embryos. Mol. Reprod. Dev., 51:53-58   DOI   ScienceOn
6 Rall WF. 1987. Factor affecting the survival of mouse embryos cryopreserved by vitrification. Cryobiol., 24:387-402   DOI   PUBMED   ScienceOn
7 Kasai M, Komi JH, Takakamo A, Tssnoda H, Sakurai T and Machida T. 1990. A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J. Reprod. Fertil., 89:91-97   DOI
8 Gunzel. AR. 1986. Semen collection, evaluation, preservation and artificial insemination in the dog. Tierarzti Prax., 14:275-282
9 Rall WF and Fahy GM. 1985. Ice-free cryopreservation of mouse embryos at -196$^\circ C$ by vitrification. Nature, 313:573-575   DOI   ScienceOn
10 Kono T, Kwon OY and Nakahara T. 1991. Development of vitrified mouse oocytes after in vitro fertilization. Cryobiology, 28:50-54   DOI   ScienceOn
11 Tachigawa S, Otoi T, Kondo S, Machida T and Kasa M. 1993. Successful vitrification of bovine blastocyst, derived by in vitro maturation and fertilization. Mol. Rerpod. Dev., 34:266-271   DOI   ScienceOn
12 Schellander K, Brackett BK, Fuher F and Schleger W 1988. In vitro fertilization of frozen-thawed cattle oocytes. Proc 11th Congra. on Anim. Reprod. and June, A.I., Dublin Ireland, 26-30
13 Vajta G, Lewis I. M, Kuwayama M, Greve T and Callesen H. 1998b. Steril application of the open pulled straw(OPS) vitrification method. Cryo-Letters, 19:389-392
14 Dinnyes A, Wallace GA and Rall WF. 1995. Effect of genotype onthe efficiency of mouse embryo cryopreservation by vitrification of slow freezing methods. Mol. Rerpod. Dev., 40:429-435   DOI   ScienceOn
15 Kim SK. 2001. Studies on the viability of frozen removed seminal plasma by saline(RSP-S) and tris-buffer(RSP-T) semen of small species dogs. Korean J. Anim. Reprod., 25(3):269-275
16 Riha J, Landa V, Kneisssl J, matus J, Jindra J and Kloucek Z. 1991. vitrification of cattle embryos by direct dropping into liquid nitrogen and embryo survival after non-surgical transfer. Ziivoc. Vir., 36:113-120