• Journal of Biosystems Engineering
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• 제25권6호
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• pp.517-526
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• 2000

A series of experiments was conducted to evaluate the capability of detecting nutrimental deficient stress of N, P and Ca of a tomato plant using several fast and intact type physiological properties measuring devices - a chlorophyll content meter an infra-red thermometer to measure leaf temperature a chlorophyll fluorescence meter a porometer an optical spectrometer a multi-scan radiometer and a canopy analyzer. to detect N deficient stress a chlorophyll content meter a spectrometer and a multi-scan radiometer were useful and their possibility to detect was estimated as about 50%, 100% and 100% respectively. To detect P deficient stress the infra-red thermometer the porometer and the spectrometer proved their usefulness an their possibility to detect was estimated as about 70%, 70% and 70% respectively. To detect Ca deficient stress an thermometer a porometer a spectrometer and a multi-scan radiometer were useful and their possibility to detect was estimated as about 60%, 70%,80% and 100% respectively. The experiments resulted that use of a spectrometer and a multi-scan radiometer in combination with a chlorophyll content meter an infra-red thermometer and a porometer were desirable to distinguish the nutrimental stress tested in the study.

• 한국산학기술학회논문지
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• 제15권7호
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• pp.4184-4190
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• 2014

본 연구의 목적은 요양시설 입소노인의 일반적 특성에 따른 스트레스와 생활행동지수를 파악하고자 한다. 연구대상은 서울시 N구에 거주하는 입소노인과 강원도 횡성군 S면 거주하는 입소노인 110명 대상으로 2013년 5월 1일부터 9월30일까지 설문조사를 실시하였다. 자료 분석은 SPSS/WIN 18.0 프로그램을 이용하여 t-test, 상관분석, 다중회귀분석방법 등을 실시하였고, 실증분석은 유의수준 5%에서 검증하였다. 분석결과는 첫째, 입소 증후군은 일상적 스트레스의 가족왕래부족 스트레스와 정의 상관이 있었다. 둘째, 우울증상은 일상적 스트레스의 개인시공간부족, 가족왕래부족 스트레스와 정의 상관이 있었다. 셋째, 체중조절은 일상적 스트레스의 개인시공간부족 스트레스와만 정의 상관이 있었으며, 영양결핍증상은 일상적 스트레스의 가족왕래부족 스트레스와만 정의 상관이 있었다. 본 연구의 시사점은 입소노인의 일반적 특성에 따른 스트레스와 생활행동지수 정책의 기초자료가 될 것으로 사료된다.

• Journal of Electrical Engineering and Technology
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• 제1권4호
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• pp.414-422
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• 2006

Deterministic techniques have been applied in power system planning for many years and there is a growing interest in combining these techniques with probabilistic considerations to assess the increased system stress due to the restructured electricity environment. The overall reliability framework proposed in this paper incorporates the deterministic N-1 criterion in a probabilistic framework, and results in the joint inclusion of both adequacy and security considerations in system planning. The combined framework is achieved using system well-being analysis and traditional adequacy assessment. System well-being analysis is used to quantify the degree of N-1 security and N-1 insecurity in terms of probabilities and frequencies. Traditional adequacy assessment is Incorporated to quantify the magnitude of the severity and consequences associated with system failure. The concepts are illustrated by application to two test systems. The results based on the overall reliability analysis framework indicate that adequacy indices are adversely affected by a generation deficient environment and security indices are adversely affected by a transmission deficient environment. The combined adequacy and security framework presented in this paper can assist system planners to realize the overall benefits associated with system modifications based on the degree of adequacy and security, and therefore facilitate the decision making process.

• Applied Biological Chemistry
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• 제40권3호
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• pp.232-237
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• 1997

인산 결핍이 질소고정식물체의 Ureide 농도 및 분배에 미치는 영향을 살펴보고자 Bradyrhizobium japonicum MN 110을 접종한 대두 식물체를 1.0 mM-P(대조구) 및 0.05 mM-P(결핍구)를 함유한 무질소 영양액을 처리, 재배하여 이식 28, 35,42, 49일 후 수확하였다. 3주의 실험기간중 건물량은 대조구의 경우 8.9배, 결점구의 경우 2.7배 증가하였다. 인산 결핍구에서 식물체의 전질소 및 인산의 함량은 이식 후 28일에 각각 80%,40%,49일에 각각 93%, 84% 감소하였으며 뿌리 및 근류보다 잎과 줄기의 질소 농도가 크게 감소하였다. 인산 결핍은 잎과 줄기의 수용태 및 ureide태 질소 농도를 감소시켰으나 뿌리의 경우 영향을 받지 않았으며 인산결점구의 경우 전체 수용태 및 ureide태 질소의 함량중 뿌리 함유량 비율이 대조구에 비하여 증가하였다. 이러한 결과는 인산 결핍이 근류의 질소고정뿐만 아니라 고정질소의 상향 이동도 저해함을 나타낸다.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1999년도 하계 학술대회 논문집
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• pp.171-176
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• 1999

작물의 생육장해 진단은 식물체의 계측에 의해 수행되는데, 이러한 계측은 생육 중인 작물을 대상으로 할 경우, 작물의 잎이나 줄기의 비파괴적 생체(in-vivo)계측을 필요로 하며 비접촉이면 더욱 바람직하다. 그리고 식물체의 건강상태를 좀더 신속하고 정확히 파악하기 위해서는 가능한 다양한 항목을 측정 대상으로 하여 관찰하는 것이 요구될 뿐만 아니라 많은 경우 그 관찰은 미소한 생장반응 및 생장량을 측정하여야 하므로 상당히 높은 정확도의 전문 센서가 필요하다. (중략)

• Molecules and Cells
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• 제21권3호
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• pp.356-359
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• 2006

The transcription factor E2F1 coordinates cell cycle progression and induces apoptosis in response to DNA damage stress. Aside from DNA damage, the role of E2F1 in the endoplasmic reticulum (ER) stress signaling pathways is unclear. We found that $E2F1^{-/-}$ murine embryonic fibroblasts (MEFs) are resistant to apoptosis triggered by the ER stress inducer thapsigargin. In addition, E2F1 deficiency results in enhanced phosphorylation of eukaryotic translation initiation factor $2{\alpha}$ ($elF2{\alpha}$). These results therefore indicate that E2F1 deficiency increases phosphorylation of $elF2{\alpha}$ in response to ER stress triggered by thapsigargin, and suggest that the reduction in ER stress-induced apoptosis in E2F1-deficient cells is related to the high level of $elF2{\alpha}$ phosphorylation.

• 한국작물학회지
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• 제46권4호
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• pp.272-278
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• 2001

To investigate of C and N metabolisms in response to phosphorus-deficient stress during regrowth of Italian ryegrass (Lolium multiflorum L.), C and N metabolites were analyzed at 0, 6, 12 and 24 days after defoliation. P-sufficient (control, +P) and P-absent (-P) nutrient solutions were applied from 7 days before defoliation, and continued for one cycle of 24 day-regrowth period. During 24 days of regrowth, dry matter of regrowing shoots and remaining tissues were not significantly different between +P and -P treatment. In remaining stubble, 70% to 91 % decline of the initial level (at day 0) in all C compounds occurred during the first 6 days of regrowth. Initial amounts of nitrate and amino acids in roots were significantly higher in the +P medium. Nitrate contents in stubble in the +P medium significantly decreased for the first 12 days and then rapidly recovered, while that of the -P medium continuously decreased until day 24. Amino acids in stubble in the P medium were continuously increased during the whole regrowth period. Soluble proteins in stubble in the +P medium also largely fell down (46.0% of the initial) for only the first 6 days, however the decline in the -P medium continued until day 12. In regrowing shoots, the accumulation of C compounds was significantly higher, while that of N compounds except amino acids was largely lower in the -P medium. These results showed a stimulation of carbohydrate synthesis and a compensatory utilization of organic reserves occurred to support regrowth under P-deficient condition.

• 한국초지조사료학회지
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• 제21권2호
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• pp.81-88
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• 2001

To investigate C and N metabolisms in response to potassium-deficient stress during regrowth of Italian ryegrass(Lolium multiflorum L.), C and N metabolites were analyzed at day 0 (cutting date), 6, 12 and 24 days after defoliation. K-sufficient (control, +K) and K-absent (-K) nutrition solutions were applied from 7 days before defoliation, and continued for one cycle of 24 days-regrowth period. During 24 days of regrowth dry matter of regrowing shoots and remaining tissues were not significantly different between +K and -K treatment. In remaining stubble, all C compounds in both +K and -K treatment largely decreased (69% to 84% of the initial level) during the first 6 days of regrowth, and then rapidly recovered. The decline of soluble sugars and fructan in roots for the first 6 days much less in the -K medium. Amino acids, soluble and insoluble proteins in stubble also feel down during the first 6 days, thereafter actively replenished in both +K and -K treatment. The decline of nitrate in stubble prolonged to 12 days of regrowth. Initial amounts of all N compounds in roots were significantly lower in the -K medium. Higher accumulation of amino acids and soluble protein in roots in the -K medium was observed after 12 days of regrowth. In regrowing shoots, 3 all carbohydrates increased with a very similar pattern for both treatments. Nitrate was not significantly different between two treatments. Depress of soluble protein accumulation in -K medium was noteworthy after 12 days of regrowth. These results indicated that an active utilization of organic reserves occurred to support regrowth even under K deficient condition with a similar extent with K sufficient condition.

• Journal of Biosystems Engineering
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• 제24권6호
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• pp.539-546
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• 1999

오이를 공시재료로 하여, 생육중인 식물 잎으로부터 비교적 신속하게 생체정보 수집이 가능한 비파괴 계측기인 엽록소 측정기, 엽록소 형광측정기, 적외선 엽온측정기, 반사형 분광분석기를 이용하여 질소 결핍장해 오이의 조기 진단 가능성 여부와 그 유용한 정도를 파악하기 위하여 수행한 연구의 주요 결과는 다음과 같다. 1) 엽록소 측정기의 측정값으로서 질소 결핍장해 오이의 진단은 엽록소 함량 45 SPAD 이하 여부로서 판단할 수 있고, 이에 의한 질소 결핍 장해의 진단은 장해 발생 후 빠르면 3일 정도면 적중율 95%수준의 장해 진단이 가능하고 7∼10일 후엔 높은 정확도의 진단이 가능함을 알 수 있었다. 2) 엽록소 형광 측정에 의한 질소 결핍장해 오이의 진단은 장해 발생 후 빠르면 5일째부터 적중률 95%수준의 장해 진단이 가능하며, 이에 의한 오이의 질소 결핍장해 진단은 엽록소 함량 측정에 의한 진단보다는 우수하지 못하다. 그리고 대기-엽온차에 의해 질소 결핍장해 오이의 진단은 불가능한 것으로 판단되었다. 3) 분광분석기의 흡광도 분석에 의한 질소 결핍 진단의 민감 파장대는 562∼564 nm, 700∼724 nm, 1,886∼1,894 nm으로 분석되었다. 이러한 영역의 파장에 의한 질소 결핍장해 오이의 진단은 장해 발생후 3∼4일째부터 적중을 95%수준의 진단이 가능하며, 진단의 정확도는 본 연구에서 사용한 4가지 측정기 중 가장 우수하였다.

• Journal of Plant Biotechnology
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• 제39권2호
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• pp.106-113
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• 2012

Glycolysis is responsible for the conversion of glucose into pyruvate and for supplying reducing power and several metabolites. Fructose-1,6-bisphosphate aldolase (AtFBA1), a central enzyme in the glycolysis pathway, was isolated by functional complementation of the salt-sensitive phenotype of a calcineurin (CaN)-deficient yeast mutant. Under high salinity conditions, aldolase activity and the concentration of NADH were compromised. However, expression of AtFBA1 maintained aldolase activity and the NADH level in yeast cells. AtFBA1 shares a high degree of sequence identity with known class I type aldolases, and its expression was negatively regulated by stress conditions including NaCl. The fusion protein GFP-AtFBA1 was localized in the cytosol of Arabidopsis protoplasts. The seed germination and root elongation of AtFBA1 knock-out plants exhibited sensitivity to ABA and salt stress. These results indicate that AtFBA1 expression and aldolase activity is important for stress tolerance in yeast and plants.