• The Korean Journal of Malacology
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• v.23 no.2
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• pp.199-208
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• 2007

The gonad index, gonadosomatic index, the condition index, reproductive cycle, first sexual maturity, and sex ratio of the hard shelled mussel, Mytilus coruscus were investigated by using histological analysis and morphometric data. Specimens were collected monthly in Kyeokpo, western Korea from January to December, 2006. The gonad index reached a maximum in February, and minimum in September. Both the monthly variations of the gonad index (GI) and the gonadosomatic index (GSI) reached a maximum in February. After that, their values continually decreased between March and April because of spawning. The monthly variation of the condition index coincides with the GI and the GSI. The spawning period was from February to April, with the main spawning occurring between February and March. The reproductive cycle of this species could be classified into six stages: early active stage (November to January), late active stage (November to February), ripe stage (December to April), partially spawned stage (February to April), degenerative stage (April to October), and resting stage (June to December ). Percentages of sexual maturity was over 50% for the female and male hard shelled mussels that ranges from 40.1 to 50.0 mm in shell length. The percentage was 100% for those that are over 50.1 mm. The sex ratios of females to males over 40.1 mm in shell length were not significantly different from a 1:1 sex ratio $(x^2\;=\;0.63,\;p\;>\;0.05)$. No evidence of hermaphroditism was found in histological sections of any hard shelled mussel examined.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.7 no.2
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• pp.87-90
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• 1974

The followings are the results of study about morphological variation of mussel, Mytilus coruscus collected respectively from Sanyang-myeon Tongyeong-goon Gyeongnam on 13th January, Yeonwha-do Yokji-myeon Tongyeong-goon Gyeongnam on 15th May and down below the Yeong-do Bridge Busan on 20th August, 1964. The biggest mussel of the above three areas have been from Yeong-do Bridge, whose shell height is 164.1 mm, shell length 77.8mm, shell breadth 52.2mm, total weight 291,9g and shell length is as follows : from Yeong-do Bridge L=0.4954H + 1.9516, from Sanyang-myeon L=0.3718H + 14.145, from Yeonwha-do L=0.4074H + 9,6610 The relationship between shell height and shell breadth is as follows : from Yeong-do Bridge B=0.3426H + 0.2052, from Sanyang-myeon B=0.3084H + 3.6183, from Yeonwha-do B=0.3507H + 0.8028 In view of the above relationship, it is concluded that the slope value of mussel, nearer to the inshore from the off-sea, is similar to that of M. edulis, from which are can presume that M. coruscus could be changed in shell form according to its environment. Growth curve between total weight and shell height is as follows : from Yeong-do Bridge W=0. 00020469 $$H^{2.79745}$ from Sanyang-myeon W=0.00061512$H^{2.53708}$ from Yeonwha-do W=0.00016965$H^{2.83960}$

• Journal of Life Science
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• v.27 no.11
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• pp.1276-1289
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• 2017

This study isolated and purified the antimicrobial peptide McSSP-31 from an acidified hemocyte extract of a Mytilus coruscus. The antimicrobial peptide was purified by using a $C_{18}$ reversed-phase high-performance liquid chromatography (HPLC). The peptide was determined to be 3330.549 Da by matrix assisted-laser desorption ionization time-of-flight mass spectrophotometer (MALDI-TOF/MS). The N-terminus of a 14 amino-acid sequence was identified as P-S-P-T-R-R-S-T-S-R-S-K-S-R by Edman degradation method. The acquired sequence showed a 93% similarity with the sperm-specific protein Phi-1, which is from M. californianus. The identified open-reading frame (ORF) of peptide was 306 bp encoding 101 amino acids, which was analyzed by rapid amplification of cDNA ends (RACE), cloning and sequencing analysis. We compared the full sequence with other known proteins that reveal the sperm-specific protein Phi-1 (93.5%) of M. californianus. Synthesized antimicrobial peptide (McSSP-31) showed antibacterial activity against gram-positive bacteria including B. subtilis, S. mutans, S. aureus and gram-negative bacteria including E. coli, K. pneumoniae, P. mirabilis, P. aeruginosa and fungi, C. albicans. Also, synthesized peptide showed strong antibacterial activity against antibiotic-resistant strains, including S. aureus. The cytotoxicity of the peptide was determined by using the HUVEC human cell line. The peptide did not exhibit any significant cytotoxic effects on the normal human cell line, and it had very low hemolytic activity with flounder hemoglobin. The results demonstrated that peptide purified from the hemocyte of a M. coruscus exhibits antibacterial activity against various bacteria and has the potential to be an alternative antibiotic agent.

• Korean Journal of Food Science and Technology
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• v.20 no.3
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• pp.385-391
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• 1988

In an attempt to develop natural seasoning materials by use of shellfishes and crustaceans, contents of taste components such as amino acids, nucleotide and its derivatives, the extractability of oyster (Crassostrea gigas), sea mussel(Mytilus coruscus) and crab(Portanus tribuerculata) were investigated. As a result of chemical analysis and sensory evaluation, the optimum condition of extraction could be concluded as extracting fresh or frozen raw materials for about 40 min. at $95^{\circ}C$ with 1.5 to 2 times of water by volume. The contents of free amino acids in the extractions were much in sequence as crab(1,886 mg%), mussel(765 mg%) and oyster(554 mg%), and the dominant amino acids in each extracts were identified as glutamic acid, alanine, glycine, proline and arginine in oyster, threonine, alanine, arginine, glycine and glutamic acid in mussel, arginine, proline, lysine, alanine and threonine in crab respectively. In addition, the major nucleotides affecting as taste enhancer of each extracts were estimated as inosine in oyster and crab, and inosine monophosphate in mussel respectively.

• Korean Journal of Food Science and Technology
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• v.30 no.1
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• pp.206-212
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• 1998

This study was undertaken to explore the applicability of glutathione S-transferase (GST) activity as a predictable indicator to monitor chemical pollution in shells and fishes utilized for food. There were some variations in the basal level of GST activity depending on species tested. Ark shells, Anadara satowi, showed the highest normal enzyme activity, followed by catfish and marine mussels, Mytilus coruscus. White clams, Meretrix lusoria, Israeli carp and catfish had lower activity. When A. satowi was exposed to 3-methyl-cholanthrene (3-MC), a prototypic polycyclic aromatic hydrocarbon for 1 week, GST activity decreased by about 30%. This reduction in GST activity induced by 3-MC did not recover until two weeks after the cessation of exposure. GST activity increased in response to 3-MC in most of the other species studied. The GST elevation in M. coruscus attained its maxinum of about 200% at the termination of 3-MC exposure maintaining this level up to 2 weeks, and declined gradually thereafter. 3-MC also induced GST activity in lsraeli carp in a similar fashion to M. coruscus. Phenobarbital induced GST activity both in M. coruscus and lsraeil carp. Other chemicals. such as clofibrate, butylated hydroxyanisole. hexachlorobenzene, and oxolinic acid did not change the enzyme activity significantly in most speciel. Phenol depressed GST activity only in lsraeli carp. These results suggest that the basal level of GST activity is somewhat variable and that the direction of change in response to chemicals seems to be related to its normal activity. The change in enzyme activity can be a preditable indicator of some environmental chemicals such as PAHs and phenol.

• Journal of Microbiology and Biotechnology
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• v.22 no.11
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• pp.1596-1596
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• 2012

• The Korean Journal of Malacology
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• v.32 no.3
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• pp.203-209
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• 2016

Growth and survival of the hard shelled mussel spat were investigated to confirm the possibe spat culture in seawater pond from September 2014 to April 2015. Also, we measured simultaneously environmental factors including water temperature, salinity, dissolved oxygen, pH, DIN, DIP, chlorophyll a, and abundance and dominant species of phytoplankton in seawater pond every month. Ranges of water temperature and salinity were $4.0-23.4^{\circ}C$ and 18.8-25.2 psu, respectively, which were rather lower than the reported optimal level. Monthly measured survival rates in all the spat cages were over 90%. Concentration of chlorophyll-a and abundance of phytoplankton were very high, and dominant species phytoplankton were cryptomonads and nanoflagellates. These dominant species were considered to be good food organisms for the bivalve spat. The experimental cage stocked 100 individuals per basket ($2,700ind.\;m^{-2}$) hanging in bottom of seawater pond revealed the highest growth in shell height ($7.63{\pm}4.65mm$), but all experimental cages stocked below 200 individuals per basket ($5,400ind.\;m^{-2}$) did not show statistically significant difference. We may expect that seawater pond would be one of the best culture ground for bivalve spats when appropriate measures are available.

• Korean Journal of Environmental Biology
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• v.28 no.4
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• pp.179-187
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• 2010

The Theory of Island Biogeography describes that the number of species on an island affected by island area and distance from the mainland. This study was performed to compare and analyze the community structure of the macro-invertebrates in three isolated islands, around Korean waters in terms of the Theory of Island Biogeography. Macrobenthic animals were collected using a modified underwater quadrat in August 2009. A total of 104 macrobenthic species were sampled with a mean density of 399 individuals $m^{-2}$ and biomass of 1,506.70 g $m^{-2}$. Based on the abundance and biomass data, there were 10 dominant species accounting for approximately 67.17% of total individuals. The highest densities were found in the amphipoda Amphipoda spp., the bivalvia Modiolus agripetus and Mytilus coruscus, the Sipunculida Phascolosoma scolops and the polychaeta Syllidae unid.. On the contrary, the top ten species made up 95.66% of the total biomass while the three most abundant, the bivalves M. coruscus, Streostria circumpicta and M. agripetus. The conventional multi-variate statistics (cluster analysis and non-metric multi-dimensional scaling) applied to assess spatial variation in macrobenthic assemblages. Cluster analysis and nMDS ordination analysis based on the Bray-Curtis similarity identified 2 station groups. The group 1 was consisted with Gageodo (except for lower station at Transect 2) and Dokdo station and was numerically dominated by the polychaetes Eunice antennata and Syllidae unid., the cirripedia Megabalanus rosa and the bivalvia M. coruscus. However, group 2 was associated with Sohwado station and was characterized by high abundance of the anomura Petrolisthes japonicus, the gastropoda Lirularia pygmaea and the brachiopoda Coptothyris grayi. In conclusion, these results suggested that the species diversity and community structure of macrobenthos in three isolated island seemed slightly related to island area and distance from the mainland.

• Korean Journal of Food Science and Technology
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• v.22 no.3
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• pp.234-240
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• 1990

Attempts have been made to optimize the hydrolysis conditions of the oyster and the mussel by the commercial proteolytic enzymes. Raw materials were digested with seven different commercial enzymes, and their quality parameters measured in terms of degree of hydrolysis and content of free amino nitrogen, nucleic acid-related substances. and free amino acids as well as sensory evaluation of optimization of their hydrolysis conditions. As a result, following enzymes have been disclosed as effective for enzymatic digestion: MKC-HT proteolytic, alcalase 0.6L and thermease for the oyster whereas MKC-acid fungal protease and thermoase for the mussel, respectively.

• Biomedical Science Letters
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• v.28 no.2
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• pp.83-91
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• 2022

This study was performed to investigate the contamination status of microorganisms that cause food poisoning in shellfish distributed in the metropolitan area of South Korea. The analyses were conducted according to the sample type, season, and region. In particular, the genotype of food poisoning viruses that explosively cause collective food poisoning was analyzed. Total 483 each of four types of shellfish (Crassostrea gigas, Mytilus coruscus, Pectea albicans albicans, and Scapharca subcrenata) were collected from four distribution sites located in the metropolitan area. We investigated foodborne pathogen by multiplex PCR and RT/Nested PCR from shellfish. As a result, Vibrio parahaemolyticus and Bacillus cereus were detected in 13 and 21 samples and Norovirus (NoV) GI and GII were detected in 7 and 12 samples, respectively. V. parahaemolyticus and NoV GI and GII showed differences in types of shellfish and seasons, and no correlation was confirmed with regional differences. Also, as a result of genotyping for the detected NoV GI and GII, they were confirmed as NoV GI.7, GI.5 and GII.3, which are causes of collective food poisoning. Therefore, cross-infection with shellfish can cause food poisoning. In particular, attention must be paid to the handling and cooking of shellfish in organizations that implement group feeding, and it is necessary to establish a management system for microorganisms that cause food poisoning in the process of shellfish farming and distribution.