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Genetic Diversity of Foodborne Pathogen Detected in Commercial Shellfish in Metropolitan Area

  • Park, Jung Hun (Department of Biomedical Laboratory Science, Shinhan University) ;
  • Cho, Kyu Bong (Department of Biomedical Laboratory Science, Shinhan University)
  • Received : 2022.05.11
  • Accepted : 2022.06.16
  • Published : 2022.06.30

Abstract

This study was performed to investigate the contamination status of microorganisms that cause food poisoning in shellfish distributed in the metropolitan area of South Korea. The analyses were conducted according to the sample type, season, and region. In particular, the genotype of food poisoning viruses that explosively cause collective food poisoning was analyzed. Total 483 each of four types of shellfish (Crassostrea gigas, Mytilus coruscus, Pectea albicans albicans, and Scapharca subcrenata) were collected from four distribution sites located in the metropolitan area. We investigated foodborne pathogen by multiplex PCR and RT/Nested PCR from shellfish. As a result, Vibrio parahaemolyticus and Bacillus cereus were detected in 13 and 21 samples and Norovirus (NoV) GI and GII were detected in 7 and 12 samples, respectively. V. parahaemolyticus and NoV GI and GII showed differences in types of shellfish and seasons, and no correlation was confirmed with regional differences. Also, as a result of genotyping for the detected NoV GI and GII, they were confirmed as NoV GI.7, GI.5 and GII.3, which are causes of collective food poisoning. Therefore, cross-infection with shellfish can cause food poisoning. In particular, attention must be paid to the handling and cooking of shellfish in organizations that implement group feeding, and it is necessary to establish a management system for microorganisms that cause food poisoning in the process of shellfish farming and distribution.

Keywords

References

  1. Ahn SJ, Chung HY, Lim SY, et al. Complete genome of Vibrio parahaemolyticus FORC014 isolated from the toothfish. Gut Pathog. 2016. 8: 59. https://doi.org/10.1186/s13099-016-0134-0
  2. Becker-Dreps S, Bucardo F, Vinje J. Sapovirus: an important cause of acute gastroenteritis in children. Lancet Child Adolesc Health. 2019. 3: 758-759. https://doi.org/10.1016/S2352-4642(19)30270-6
  3. Bellou M, Kokkinos P, Vantarakis A. Shellfish-borne viral outbreaks: a systematic review. Food Environ Virol. 2013. 5: 13-23. https://doi.org/10.1007/s12560-012-9097-6
  4. Bull RA, Tu ET, Mclver CJ, et al. Emergence of a new norovirus genotype II.4 variant associated with global outbreaks of gastroenteritis. J Clin Microbiol. 2006. 44: 327-333. https://doi.org/10.1128/JCM.44.2.327-333.2006
  5. Cheng HY, Hung MN, Chen WC, et al. Ice-associated Norovirus outbreak predominantly caused by GII.17 in Taiwan, 2015. BMC Public Health. 2017. 17: 870. https://doi.org/10.1186/s12889-017-4869-4
  6. Chhabra P, de Graaf M, Parra GI, et al. Updated classification of Norovirus genogroups and genotypes. J Gen Virol. 2019. 100: 1393-1406. https://doi.org/10.1099/jgv.0.001318
  7. Chiu SC, Hsu JK, Hu SC, et al. Molecular Epidemiology of GI.3 Norovirus Outbreaks from Acute Gastroenteritis Surveillance System in Taiwan, 2015-2019. Biomed Res Int. 2020. 4707538: 1-9.
  8. Choi ES, Kim NH, Lee SH, Lee MS, Rhee HJ. Seafood and Bacteria. KoSFoS. 2012. 7: 3-13.
  9. Cho HG, Lee SG, Lee MY, et al. An outbreak of norovirus infection associated with fermented oyster consumption in South Korea, 2013. Epidemiol Infect. 2016. 144: 2759-2764. https://doi.org/10.1017/S0950268816000170
  10. Cho SR, Yun SJ, Chae SJ, et al. An Outbreak Associated with Sapovirus GI.3 in an Elementary School in Gyeonggi-do, Korea. J Korean Med Sci. 2020. 35: e281. https://doi.org/10.3346/jkms.2020.35.e281
  11. de Oliveira-Tozetto S, Santiso-Bellon C, Ferrer-Chirivella JM, et al. Epidemiological and Genetic Characterization of Sapovirus in Patients with Acute Gastroenteritis in Valencia (Spain). Virueses. 2021. 184: 1-11.
  12. Dumen E, Ekici G, Ergin S, Bayrakal GM. Presence of Foodborne Pathogens in Seafood and Risk Ranking for Pathogens. Foodborne Pathog. Dis. 2020. 17: 541-546. https://doi.org/10.1089/fpd.2019.2753
  13. Elmahdi S, DaSilva LV, Parveen S. Antibiotic resistance of Vibrio parahaemolyticus and Vibrio vulnificus in various countries: A review. Food Microbiol. 2016. 57: 128-134. https://doi.org/10.1016/j.fm.2016.02.008
  14. Fu J, Ai J, Bao C, et al. Evolution of the GII.3[P12] Norovirus from 2010 to 2019 in Jiangsu, China. Gut Pathog. 2021. 13: 34. https://doi.org/10.1186/s13099-021-00430-8
  15. Han TH, Chung JY. Recent advances in Norovirus infection. Journal of the Korean Medical Association. 2017. 60: 985-991. https://doi.org/10.5124/jkma.2017.60.12.985
  16. Ho ZJ, Vithia G, Ng CG, et al. Emergence of Norovirus GI.2 outbreaks in military camps in Singapore. Int J Infect Dis. 2015. 31: 23-30. https://doi.org/10.1016/j.ijid.2014.12.023
  17. Hsu TK, Tsai HC, Hsu BM, Yang YY, Chen JS. Prevalence, enterotoxin-gene profiles, antimicrobial resistance, and genetic diversity of Bacillus cereus group in aquatic environments and shellfish. Sci Total Environ. 2021. 758: 143665. https://doi.org/10.1016/j.scitotenv.2020.143665
  18. Kang CH, Shin YJ, Kim WR, et al. Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from oysters in Korea. Environ Sci Pollut Res Int. 2016. 23: 918-926. https://doi.org/10.1007/s11356-015-5650-9
  19. Kang CR, Kim YY, Lee JI, et al. An Outbreak of Scombroid Fish Poisoning Associated with Consumption of Yellowtail Fish in Seoul, Korea. J Korean Med Sci. 2018. Sci. 33: e235. https://doi.org/10.3346/jkms.2018.33.e235
  20. Kim HW, Hong YJ, Jo JI, et al. Raw ready-to-eat seafood safety: microbiological quality of the various seafood species available in fishery, hyper and online markets. Lett Appl Microbiol. 2017. 64: 27-34. https://doi.org/10.1111/lam.12688
  21. Kim SY, Chung HY, Lee DH, et al. Complete genome sequence of Vibrio parahaemolyticus strain FORC 008, a foodborne pathogen from a flounder fish in South Korea. Pathog Dis. 2016. 74: ftw044. https://doi.org/10.1093/femspd/ftw044
  22. Koo HJ, Kwak HS, Yoon SH, et al. Phylogenetic group distribution and prevalence of virulence genes in Escherichia coli isolates from food samples in South Korea. World J Microbiol Biotechnol. 2012. 28: 1813-1816. https://doi.org/10.1007/s11274-011-0954-5
  23. Kwun JW, Lee CH. Trends of Recent Food-Borne Disease Outbreaks in Korea. Journal of the Korean Medical Association. 2007. 50: 573-581. https://doi.org/10.5124/jkma.2007.50.7.573
  24. La Bella G, Martella, V, Basanisi MG, et al. Food-Borne Viruses in Shellfish: Investigation on Norovirus and HAV Presence in Apulia (SE Italy). Food Environ Virol. 2017. 9: 179-186. https://doi.org/10.1007/s12560-016-9273-1
  25. Lee S, Bae KS, Lee JY, et al. Development of Molecular Diagnostic System with High Sensitivity for the Detection of Human Sapovirus from Water Environments. Biomed Sci Letters. 2021. 27: 35-43. https://doi.org/10.15616/BSL.2021.27.1.35
  26. Lee TS, Oh EG, Yoo HD, et al. Impact of Rainfall Events on the Bacteriological Water Quality of the Shellfish Growing Area in Korea. Korean J Fish Aquat Sci. 2010. 43: 406-414. https://doi.org/10.5657/KFAS.2010.43.5.406
  27. Lee Y, Choi Y, Lee S, et al. Occurrence of pathogenic Vibrio parahaemolyticus in seafood distribution channels and their antibiotic resistance profiles in S. Korea. Lett Appl Microbiol. 2019. 68: 128-133. https://doi.org/10.1111/lam.13099
  28. Li J, Gao X, Ye YL, et al. An acute gastroenteritis outbreak associated with person-to-person transmission in a primary school in Shanghai: first report of a GI.5 Norovirus outbreak in China. BMC Infect Dis. 2018. 18: 316. https://doi.org/10.1186/s12879-018-3224-4
  29. Li JY, Mai W, Tan HQ, et al. An outbreak of acute gastroenteritis caused by Sapovirus in a community of Guangdong province. Zhonghua Liu Xing Bing Xue Za Zhi. 2020. 41: 226-230.
  30. Lopatek M, Wieczorek K, Osek J. Prevalence and Antimicrobial Resistance of Vibrio parahaemolyticus Isolated from Raw Shellfish in Poland. J Food Prot. 2015. 78: 1029-1033. https://doi.org/10.4315/0362-028X.JFP-14-437
  31. Marques Mendanha de Oliveira D, Souza M, Souza Fiaccadori F, Cesar Pereira Santos H, das Dores de Paula Cardoso D. Monitoring of Calicivirus among day-care children: evidence of asymptomatic viral excretion and first report of GI.7 Norovirus and GI.3 Sapovirus in Brazil. J Med Virol. 2014. 86: 1569-1575. https://doi.org/10.1002/jmv.23791
  32. Oh EG, Yoo HD, Yu HS, et al. Removal of Fecal Indicator Bacteria from Bivalves under Natural and Electrolyzed Water. Korean J Fish Aquat Sci. 2012. 45: 11-16. https://doi.org/10.5657/KFAS.2012.0011
  33. Park KS, Jeong HS, Baek KA, et al. Genetic analysis of norovirus GII.4 variants circulating in Korea in 2008. Arch Virol. 2010. 1550: 635-41.
  34. Park WJ, Ryu HY, Lim GY, Lee YD, Park JH. Microbial Prevalence and Quality of Organic Farm Produce from Various Production Sites. Korean J Food Sci Technol. 2014. 46: 262-267. https://doi.org/10.9721/KJFST.2014.46.2.262
  35. Rahmati T, Labbe R. Levels and toxigenicity of Bacillus cereus and Clostridium perfringens from retail seafood. J Food Prot. 2008. 71: 1178-1185. https://doi.org/10.4315/0362-028X-71.6.1178
  36. Seo DJ, Son NR, Seo SW, et al. Seafood and Viruses. Safe Food. 2012. 7: 14-23.
  37. Shin BS, Oh EG, Lee HJ, et al. Norovirus Quantification in Oysters Crassostrea gigas Collected from Tongyeoung, Korea. Korean J Fish Aquat Sci. 2014. 47: 501-507. https://doi.org/10.5657/KFAS.2014.0501
  38. Widdowson MA, Cramer EH, Hadley L, et al. Outbreaks of acute gastroenteritis on cruise ships and on land: identification of a predominant circulating strain of norovirus--United States, 2002. J Infect Dis. 2004. 190: 27-36. https://doi.org/10.1086/420888
  39. Xie T, Xu X, Wu Q, Zhang J, Cheng J. Prevalence, Molecular Characterization, and Antibiotic Susceptibility of Vibrio parahaemolyticus from Ready-to-Eat Foods in China. Front Microbiol. 2016. 7: 549. https://doi.org/10.3389/fmicb.2016.00549