• Journal of the Korean Chemical Society
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• v.15 no.3
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• pp.127-132
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• 1971

To obtain the various kinds of inosose stereomers, the process of electrochemical oxidation is more effective than chemical oxidation of myo-inositol. So that myo-inositol aqueous solution was electrolyzed by platinum and lead peroxide anode to confirming the occurrence of electrochemical oxidation. The result is that myo-inosose-2 is producing with high yield comparatively by electrolytic oxidation of myo-inositol. Also we studied about the relation between the electrolytic current efficiency and electrolytic temperature and anodic current density. The current efficiency is rising with lowering of electrolytic temperature identically in both anode such as platinum and lead peroxide and also rising with increasing of anodic current density in platinum anode, but inversely in lead peroxide.

• Mass Spectrometry Letters
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• v.7 no.1
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• pp.12-15
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• 2016

Results of free and bound myo-inositol in infant formula (IF) are presented. Inositol was analyzed by HILIC ultra-performance liquid chromatography coupled with mass spectrometer. The levels of free myo-inositol in 27 Australian and 4 EU originated IF samples were 300-600 mg/kg of powder or 1.6-3.1 mg/100 kJ. The amount of bound inositol in lipid fraction of IF was, on average, 10% of free myo-inositol.

• BMB Reports
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• v.32 no.3
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• pp.294-298
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• 1999

Myo-inositol monophosphate phosphatase is a key enzyme in the phosphoinositide cell-signaling system. Incubation of myo-inositol monophosphate phosphatase from porcine brain with N-bromosuccinimide (NBS) resulted in a time-dependent loss of enzyme activity. The inactivation followed pseudo-first-order kinetics with the second-order rate constant of $3.8{\times}10^3\;M^{-1}min^{-1}$. The time course of the reaction was significantly affected by the substrate myo-inositol-1-phosphate, which afforded complete protection against the loss of catalytic activity. Spectrophotometric studies indicated that about one oxindole group per molecule of enzyme was formed following complete loss of enzymatic activity. It is suggested that the catalytic function of myo-inositol monophosphate phosphatase is modulated by the binding of NBS to a specific tryptophan residue at or near the substrate binding site of the enzyme.

• Journal of environmental and Sanitary engineering
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• v.10 no.3
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• pp.45-55
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• 1995

This study was carried out to elucidate the toxic mechanism of cadmium in peripheral nerve. An animal model of cadmium neuropathy was induced by feeding diet containing cadmium to Sprague- Dawley rat (or two weeks. Four weeks aged Sprague- Dawley rats were divided into four groups : normal control group, 10ppm- cadmium treated group, 100ppm- cadmium treated group, 1000ppm- cadmium treated group, reference drug- treated group. All rats were sacrificed at the end of two weeks for assessing the development of cadmium neuropathy, These results obtained were summarized as follows : 1. Cadmium reduced peripheral flow of both acetylcholinesterase and cholinesterase in rat sciatic nerve. 2. The toxic mechanism of cadmium might be the result of an reduction of myo-inositol concentration in peripheral nervous system 3. Reduction in myo-inositol content of peripheral nerve resulted from the inhibition of sodium- Potassium ATPase activity, which is responsible for myo-inositol transport, by cadmium 4. Oral administration of myo-inositol improved the flow of both acetylcholinesterase and cholinesterasenerve in cadmium intoxicated rat. These results suggest that mild cadmium neuropathy might be diagnosed by checking nervous myo-inositol content and oral administraion of myo-inositol might prevent the development of severe cadmium neuropathy with special reference to detective axonal transport.

• Environmental Analysis Health and Toxicology
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• v.9 no.1_2
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• pp.1-8
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• 1994

Nerve conduction impairment in lead neuropathy has been empirically linked to altered nerve myo-inositol metabolism. In most cases of neuropathy, abnormal myo-inositol metabolism is associated with abnormal $Na^+/K^+$ATPase provides a potential mechanism to relate defects of the myo-inositol metabolism in the peripheral nerve treated with lead. Therefore, the effect of lead on the rat sciatic nerve $Na^+/K^+$ATPase and other ATPase of sciatic nerve was studied. ATPase activity was measured enzymatically in sciatic nerve homogenates from 2-wk lead treated neuropathy rats and age-mached controls administered myo-inositol. $Na^+/K^+$ATPase components were assessed by ouabain inhibition or the omission of sodium and potassium ions. Lead reduced 50% reduction in the $Na^+/K^+$ATPase activity in homogenates of sciatic nerve. The 50% reduction in the $Na^+/K^+$ ATPase activity was selectively prevented by myo-inositol treatment. This study suggests that the toxic mechanism of the lead on peripheral nerve may be through reduction in $Na^+/K^+$ATPase activity which has been linked to axonal transport slowing in the rat model of lead neuropathy, via direct changes by the perturbation of the intracelluar sodium or potasium level.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.6
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• pp.960-966
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• 2022

We aimed to determine the dietary myo-inositol (MI) requirements of Pacific white shrimp Litopenaeus vannamei. A basal diet was formulated without myo-inositol (M0) and a negative control diet (M0-) was prepared by adding tetracycline hydrochloride to the basal diet to prevent intestinal inositol synthesis. Five MI diets were prepared by adding MI at 300, 600, 900, 1,200 and 1,500 mg/kg to the basal diet (designated as, M300, M600, M900, M1200 and M1500, respectively). Triplicate groups of shrimp (initial body weight, 0.55±0.01 g) were fed one of the experimental diets for 42 days. The growth performance of shrimp in M0- group was significantly lower when compared to that of shrimp in M0, M1200 and M1500 groups. Feed efficiency was significantly improved in M1200 and M1500 groups when compared to the M0 and M0- groups. GPx activity was significantly higher in M1200 and M1500 groups compared to that in M0 and M0- groups. Therefore, a practical diet (over 240 mg/kg) meets the minimum MI requirements of Pacific white shrimp. However, the optimum dietary MI level would be potentially above 1,200 mg/kg for better feed utilization efficiency and antioxidant capacity of Pacific white shrimp.

• Journal of the Korean Chemical Society
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• v.50 no.2
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• pp.129-136
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• 2006

Synthesis of novel polymeric ligands based on myo-inositol was performed. Cyclopolymerization, whose mechanism and the cyclic structure were proved, was first attempted to build a conformationally rigid inositol polymer. Comparative spectroscopic methods were introduced to verify the conformation of the prepared cyclohexane polymers. A conformationally rigid polymeric ligand was successfully prepared using myo-inositol carbonate.

• Journal of Life Science
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• v.13 no.4
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• pp.383-389
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• 2003

A cDNA (SeMIPS) encoding myo-inositol 1-phosphate synthase has been isolated from developing sesame (Sesamum indicum L. cv. Dan-Baek) seeds and its structure and function analyzed. The SeMIPS protein was highly homologous with those from plant species (88-94%), while a much lower degree of sequence homology (60%) was found with that of human. The functional domains commonly found in MIPS protein were identified and their amino acid residues were compared with each other. Northern blot indicated that the expression of the SeMIPS gene might be organ-specifically regulated. A complementation assay based on a yeast mutant system confirmed that the SeMIPS gene encodes a myo-inositol 1-phosphate synthase (MIPS) of sesame by showing functional expression of the SeMIPS cDNA in the yeast mutants containing the disrupted INO1 gene.

• Korean Journal of Microbiology
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• v.12 no.2
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• pp.85-93
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• 1974

Four stereoisomers of inositol p-aminosalicylate, that is hexaris-O-(p-aminosalicylyl)-myo-inositol, hexaris-O-(p-aminosalicylyl)-muco-inositol were synthesized by p-aminosalicylyl chloride with myo-, epi-, scyllo-, and muco-inositol respectively. Their antibacterial activity was tested against human type tubercle bacilli $H_{37}$/Rv, in contrast to 1.25.gamma./ml of p-aminosalicylic acid used as control. Hexaris-O-(p-aminoslicylyl)-muco-inositol showed the strongest antibacterial action at 0.625.gamma./ml, the other compounds more or less active than p-aminosalicylic acid.

• Journal of Environmental Health Sciences
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• v.22 no.3
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• pp.8-16
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• 1996

Effects of water extract of aloe vera on lead-induced neurotoxicity were investigated in sciatic nerve isolated from rat. The mechanism on toxicity reduction by measuring activities of axonal enzymes, metabolism of myo-inositol in nerve, lead concentration in several organs and so on were further examimed. In the lead-treated rats, the transport rate of axonal enzyme, such as acetyl cholinesterase and choline acetyltransferase, was reduced by from 50% to 30% respectively. Reduction in myo-inositol concentration and $Na^+/K^+$ ATPase activity were also observed in sciatic nerve from lead-treated rat. However, the aloe extract administration significantly eliminated the impairment and maintained myo-inositol concentration to about 85% of normal level. Also aloe extract promoted the excretion rate of lead which is accumulated in blood, sciatic nerve and kidney. These results suggest that lead-induced neurotoxicity was significantly reduced by administration of aloe extract and the mechanism might be partly increase in kidney excretion rate of lead and parity normalization of $Na^+/K^+$ ATPase activity which is critical factor in order to keep nerve maintaining normal myo-inositol level.