• Mycobiology
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• 제35권4호
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• pp.186-190
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• 2007

This study was conducted to investigate the effects of foliar endophytic fungi and arbuscular mycorrhizal fungi (AMF) on plant community structure in experimental microcosms containing an assemblage of five species of plants (Oenothera odorata, Plantago asiatica, Trifolium repens, Isodon japonicas and Aster yomena). Leaves of Sasa borealis, Potentilia fragarioides, and Viola mandshurica were collected in Chungbuk, Korea. Endophytic fungi were isolated from the surface sterilized leaves and identified to species level using molecular and morphological techniques. Four isolates of the endophytic fungi were inoculated to the leaves of host plants in the microcosms. Also, three species of AMF spores were extracted from pure cultures and the mixture of the three species inoculated to the roots of the plants. After four months of growth in a green house, effects of both symbiotic fungi on plant species diversity, community composition and productivity were examined. The plant species diversity showed significant differences with inoculation of the symbiotic fungi. Results indicate that AMF significantly affect plant productivity and plant community structure.

• Mycobiology
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• 제45권1호
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• pp.20-24
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• 2017

We investigated the effects on various crops of inoculation with species of arbuscular mycorrhizal fungi (AMF) in soils from different sources and selected AMF species suitable for domestic environment-friendly farming. Effects on plants varied with the AMF species used. In carrot, Scutellospora heterogama, Acaulospora longula, and Funneliformis mosseae had a positive effect on growth of the host, whereas AMF had only weak effects on the growth of red pepper and leek. AMF inoculation had positive effects on the growth of carrot and sorghum. The results of this study indicate the nature of the relationship between soil, plants, and AMF; this study therefore has important implications for the future use of AMF in environment-friendly agriculture.

• Mycobiology
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• 제44권4호
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• pp.277-282
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• 2016

In this study, we collected rhizosphere soils and root samples from a post-mining area and a natural forest area in Jecheon, Korea. We extracted spores of arbuscular mycorrhizal fungi (AMF) from rhizospheres, and then examined the sequences of 18S rDNA genes of the AMF from the collected roots of plants. We compared the AMF communities in the post-mining area and the natural forest area by sequence analysis of the AMF spores from soils and of the AMF clones from roots. Consequently, we confirmed that the structure of AMF communities varied between the post-mining area and the natural forest area and showed significant relationship with heavy metal contents in soils. These results suggest that heavy metal contamination by mining activity significantly affects the AMF community structure.

• 한국균학회지
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• 제18권4호
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• pp.191-197
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• 1990

접합내생균근은 세 식물군락(차풀, 솔새, 억새)의 토양에서 분리되었다. 분리된 포자는 형태학적으로 분류 동정한 결과 4속 8종이었다. 우리나라에서 새롭게 기술하는 종(種)은 2속 6종이다 ; Glomus aggregatium, Gl. ambisporum, Gl. geosporum, Gl. mosseae, Scutellospora coralloided, and Sc. heterogama.

• 한국유기농업학회지
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• 제13권2호
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• pp.197-209
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• 2005

of Hangalku per plant sold for wild vegetables were 9.1g and 0.9g, and number of leaves was 10.8. Root fresh and dry weights of Hangalku per plant were 19.2g and 4.1g. Thirty five soil samples were collected from the native soils grown Cirsium japonicum DC., and mycorrhizal spores in soils were separated using wet-sieving methods. Number of mycorrhizal spores per 30g fresh soil sized over 500${\mu}$m, 355~500${\mu}$m, 251~354${\mu}$m, 107~250${\mu}$m and 45~106${\mu}$m were 0.6, 2.1, 6.0, 55.3 and 126, etc. Total number of mycorrhizal spores per 30g fresh soil were 190. Root infection by vesicles, hyphae and arbuscules were 13%, 4% and 3%, respectively. As a result of identification, mass propagated mycorrhizal spores by the host plant of Sudangrass were Glomus sp., Gigaspora sp., and Acaulospora sp., and so on.

• 원예과학기술지
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• 제18권6호
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• pp.802-807
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• 2000

Agrobacterium rhizogenes의 Ri t-DNA로 형질전환된 당근의 모상근에 Glomus sp.를 감염시킨후 arbuscular 균근균 포자를 Modified Strullu & Romand 배지상에서 12주 동안 기내증식하였다. 기내증식을 위한 접종원으로는 포자보다는 균근감염 뿌리가 더 좋았다. 당근 모상근과 arbuscular 균근균의 대치배양으로 증식된 Glomus sp.의 포자는 직경 약 $50{\mu}m$정도의 원형 또는 타원형의 포자로서 균사의 중간부위에서 형성되었다. 12주 동안 기내에서 대치 배양한 결과 plate 당 약 1,200개 정도의 arbuscular 균근균 포자를 생산하였다.

• Mycobiology
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• 제46권2호
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• pp.122-128
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• 2018

Arbuscular mycorrhizal fungi (AMF) are well-known for their ability to improve plant growth and help plants withstand abiotic stress conditions. Unlike other fungi and bacteria, AMF cannot be stored, as they are obligate biotrophs. Long-term preservation of AMF spores is challenging and may lead to the loss of viability and efficiency. This study aimed to understand the effect of prolonged subculture of AMF species on the growth and glomalin-related soil protein (GRSP) from red pepper (Capsicum annuum L.). AMF spores were mass-produced using different techniques and subcultured in pots with sorghum sudangrass as the host plant for 3 years. Experimental soil samples were collected from natural grassland. Five different AMF inocula were used in triplicate as treatments. After 70 days of growth, red pepper plants were harvested and plant dry weight, plant nutrient content, mycorrhizal colonization, AMF spore count, and soil glomalin content were determined. AMF-treated plants displayed higher dry weight than controls, with only fruit dry weight being significantly different. Similarly, significant differences in phosphorous and potassium contents of the above-ground plant parts were observed between mycorrhizal and control treatments. In addition, soil GRSP content was significantly higher in plants inoculated with Rhizophagus sp. and Gigaspora margarita. The increased plant growth and GRSP content suggest that AMF can be maintained for 3 years without losing their efficiency if subcultured regularly with different symbiotic host plants.

• 한국토양비료학회지
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• 제37권5호
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• pp.350-355
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• 2004

본 연구는 분무경 방식을 이용한 arbuscular 균근균 접종원의 대량배양증식을 위하여 기주식물의 종류와 분무양액의 P 농도 등에 따른 균근감염율 및 감염양상 변화 등을 비교하였다. 기주식물 선발에서는 고구마와 토마토가 수단그라스와 질경이에 비해 우수하였다. 또한 인산농도가 5 또는 $10{\mu}M$ 보다 $20{\mu}M$의 수준으로 높아질수록 뿌리의 생성량이 많아진 반면, 뿌리의 균근감염은 인산농도가 낮을수록 높고, 하부 뿌리로 갈수록 arbuscular 균근균 감염율이 낮아지는 경향을 보였으며 감염율은 18.6~26.0% 범위였다. 16주후의 포자형성을 조사한 결과 신선중 1 g 당 500개의 포자가 형성되었고, 분무생육상당 생산된 포자는 총830.479개였다.

• Journal of Microbiology and Biotechnology
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• 제2권1호
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• pp.66-71
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• 1992

The ecological distribution of endomycorrhizas in evergreen woody species native to the evergreen forest ecosystem of Tadohae-haesang National Park in southern Korea in February, 1989 was studied. The abundance and diversity of vesicular-arbuscular (VA) mycorrhizal fungi were also determined. The spore densities ranged from 14 to 326 per 100 g of soil. Most of the spores of mycorrhizal fungi collected from 25 soil samples belonged to the genera Glomus and Gigaspora. The frequency and number of spores in Camellia japonica varied with location. Spores belonging to the genus Gigaspora were not found in Camellia japonica in Yesongri evergreen forests adjacent to the sea. Glomus sp. was the major constituent of the spore assemblage at this site. The most abundant species in Camellia japonica in the Yesongri evergreen forests in Pogildo was Glomus borealis. In the soil of a mountain at Buwhangri, in the central location of the island at an elevation of 250 m, Gigaspora sp. was present and Glomus sp. was a major constituent of the spore assemblage. In the urban area of Haenam spore densities were much higher than in the Pogildo area. The most abundant species in Camellia japonica in the urban area of Haenam was Gigaspora sp..

• 한국산림과학회지
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• 제76권4호
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• pp.376-389
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• 1987

1976년(年), 1984년(年), 1985년(年), 1986년(年)의 7월(月)부터 10월(月)까지 경기도(京畿道) 광릉(光陵)에 있는 임업시험장(林業試驗場) 중부지장(中部支場)의 시험림(試驗林)에서 고등균류(高等菌類)를 채집(採集)하여 분류(分類)하였다. 총(總) 104속(屬) 257종(種)의 담자균류(擔子菌類)를 동정(同定)하였는데, 그중(中) 부생균(腐生菌)이 75속(屬), 135종(種)으로써 전체종수(全體種數)의 52%를, 외생균근성(外生菌根性)의 28속(屬) 120종(種)으로써 47%를, 기생균(寄生菌)이 1속(屬) 2종(種)으로써 1%를 점유(點有)하고 있었다. 균근(菌根)버섯 중에서 광대버섯속(屬), 무당버섯속(屬), 젖버섯속(屬)이 전체균근(全體菌根)버섯종수(種數)의 53%를(63종(種)) 차지했으며, 이는 전체(全體)버섯종수(種數)의 25%에 해당(該當)한다. 위의 세가지속(屬)의 버섯들이 광릉시험림(光陵試驗林)의 우점종(優占種)(속(屬))이라고 할 수 있다. 문헌조사결과(文獻調査結果) 257종(種) 중(中)에서 한국미기록종(韓國未記錄種)이 77종(種)에 달함을 발견(發見)하였다.