• Title/Summary/Keyword: Mycological characteristic

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Mycologic Characteristics of Trichophyton tonsurans (Trichophyton tonsurans의 진균학적 성상)

  • Bang, Young Jun;Kim, Ssang Young;Shin, Du Man
    • Korean Journal of Clinical Laboratory Science
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    • v.36 no.1
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    • pp.7-12
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    • 2004
  • Trichophyton(T.) tonsurans has been identified for the first time in Korea in 1995 and has been increasing in number of reported in wrestlers, in judo players, and in korean - style wrestlers. Author tried mycological studies with T. tonsurans is isolated in Daegu Korea. The results are as follows: All 16 strains of T. tonsurans tested for nutritional requirement, showed strongly stimulated growth by thiamine, mild to moderate growth on casamino acid agar, poor growth on ammonium nitrates base agar. All 16 strains of T. tonsurans tested showed positive results for hair perforation and urease test. Gross findings of T. tonsurans showed that fine granular surface with light yellow hue or white color and mahogany brown color on the reverse side. Microscopic findings showed that septated hyphae, chlamydoconidia and microcondia. Microconidia were characteristic in their arrangerment and shape : round or oval shaped microconidia laterally to hyphae, some were match - head like terminal swelling.

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Mycological Findings of Trichophyton tonsurans Isolated in New Orleans Area (Trichophyton tonsurans의 진균학적 소견)

  • Kim, Ki-Hong
    • Journal of Yeungnam Medical Science
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    • v.8 no.2
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    • pp.45-51
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    • 1991
  • There had been no reports of Trichophyton tonsurans infection in Korea yet. We have much chances to import the organism through international travels and exchanges. But we, Korean, have no experiences to observe T. tonsurans. Author tried mycological studies with T. tonsurans isolated in New Orleans, USA. The results are as follows : Gross findings of T. tonsurans showed that fine granular surface with light yellow hue or white color and groving in the central area on the front side and mahogany brown color on the reverse side. On the urease test, various reddish discoloration was noted. Microscopic findings showed that septated hyphae, macroconidia, chlamydoconidia and microcondia. Macroconidia were 3-5 septated, smooth-surfaced, and were found more frequently in the whitish colony. Microconidia were characteristic in their arrangement and shape ; round or oval shaped microconidia laterally to hyphae, some were match-head like terminal swelling. Hair perforation test showed positive results in 16 strains among 19 tested strains. All these findings are similar to T. rubrum and T. menatgrophytes, the most frequent isolates of dermatophytes in Korea. And we have to pay an attention to diffentiate carefully.

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Antibiotics from Mushrooms (버섯의 항생물질(抗生物質))

  • Hwang, Byung-Ho
    • Journal of Forest and Environmental Science
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    • v.22 no.1
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    • pp.83-100
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    • 2006
  • Antibiotics which produced by mushrooms discovered for last 40 years were described. Any antibiotic has not been used as infectious disease remudy but will be used as physiological active substance in near future. The antibiotic of mushrooms have not been published much in papers and do not have various finds of structures, compared to those of Streptomyces. Triple bond having compounds, terpenoid compounds aromatic compounds and some other compound have been known. These compounds are not dissolved well in water and mainly fat-soluble, except for cordycepin. Also, they are generally neutral, and some of them are acidic and almost none of them are basic compounds. However, acetylene and terpenoid compounds are the characteristic compounds of mushroom, and are not found in other microorganisms and plants. Especially, there are various terpenoid compounds in mushrooms. These metabolites of mushrooms were not used as antibiotic, but are interested as physiological active substance, such as enzyme inhibitor and immunomodulator. To promote studying on the antibiotics of mushroom, new screening methods must be developed, because strain belonged to the different genus produces different antibiotics, even though mushrooms belonged to the same genus and species. It is also known that mushrooms collected in different areas produce different antibiotics. Now, it is difficult to separate each pure compound from mushroom. It is important to find mushrooms which is impossible to cultivate artificially, or grow in the back land where is difficult to collect. Thousands of mushrooms grow on earth now, so that which species will be screened if not known. The biochemical and mycological study for usability of the metabolites of mushrooms is thought, as one of the important research areas, must be performed.

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Distribution and Classification of Cordyceps Parasites in Cicada (매미를 기주로 한 동충하초의 분포와 분류 동정)

  • Oh, Junsang;Han, Sang-Kuk;Shrestha, Bhushan;Sung, Gi-Ho;Sung, Jae-Mo
    • Journal of Naturopathy
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    • v.8 no.2
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    • pp.78-87
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    • 2019
  • Purpose: The aim was to collect and classify the Cordyceps parasitized in cicadas from July to October every year from 1990 to 2016 in Korea. And they were frequently collected in Nepal, Vietnam, Japan, China, and Thailand. Methods: Cordyceps parasitizing cicadas collected in mountains and fields. Results: A total of 1,104 specimens were collected that belonged to 10 different species under nine genera. The highest number of samples belonged to Ophiocordyceps (563 specimens), followed by Isaria (361 specimens), Polycephalomyces (73 specimens), Cordyceps (70 specimens), Beauveria (25 specimens), Perennicordyceps (8 specimens), Metarhizium (2 specimens) and Purpureocillium (2 ones). Among Ophiocordyceps spp. O. longissima was most frequently collected with a total of 426 samples out of 563, followed by O. heteropoda with 120 ones and O. sobolifera with 17 specimens. The species mainly collected in Korea, but C. ishikariensis was collected in Nepal only. The new characteristic was that Isaria cicada-like synnemata were found growing together with C. ishikariensis stromata on the same host. In Korea, the collected 691 specimens in total out of 1,104 were found in Mt. Halla in Jeju Island. Other mountains in Korea where the samples were collected were Mountains Daeryong, Jiri, Yongmoon, Samag, Seolag, Gujeol, Duryun, Baegam, Chilgap, Chundeung, Naejang, Welchul, and Daeryong. The three samples were not identified. Conclusions: A total of 1,104 specimens belonged to 10 different species under nine genera, and the collected 691 samples were found in Mt. Halla in Jeju Island.

Occurrence Dollar Spot Caused by Sclerotinia homoeocarpa in Turfgrass of Golf Course in Korea (한국 골프장에서 Sclerotinia homoeocarpa에 의한 잔디동전마름병의 발생)

  • 심규열;민규영;신현동;이현주
    • Asian Journal of Turfgrass Science
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    • v.14 no.1
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    • pp.241-250
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    • 2000
  • In 1997, a new disease on creeping bentgrass and Kentucky bluegrass occurred in the green and fairway of a few golf courses in Korea. The disease spread gradually throughout the country and became a threat to turfgrass management. Symptoms of the disease consisted of small, circular, and sunken patches sized 3~5cm in diameter. The disease named as a dollar spot following its characteristic symptoms of circular blight and bleach on the green. The disease peaked two times in a year from April to June and from late August to October and white cottony mycelia of the causal fungus developed on diseased turfs in the early morning when the conditions were favored. A causal fungus was consistently isolated from the infected tufgrass and seven isolates originated from seven golf courses located in six provinces were selected for further study. The fungus produced abundant white aerial mycelia on PDA and turned to dark gray or light brown as it aged. Width of the mycelium was ca. $5~8\mu\textrm{m}$. While sclerotia were not readily formed on the medium, scattered small and dark colored stromata were developed on the surface. The fungus grew well on PDA between 5 to $30^{\circ}C$ and maximally around $25^{\circ}C$. Based on investigated mycological and cultural characteristics, the causal agent of dollar spot was identified as Sclerotinia homoeocarpa. The fungus showed strong pathogenicity to several turfs as creeping bentgrass, Kentucky bluegrass, perennial ryegrass, tall fescues, and zoysiagrass.

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First Report of Pink Mold Rot on Tomato Fruit Caused by Trichothecium roseum in Korea (Trichothecium roseum에 의한 토마토 분홍빛열매썩음병 발생)

  • Han, Kyung-Sook;Lee, Seong-Chan;Lee, Jung-Sup;Soh, Jae-Woo
    • Research in Plant Disease
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    • v.18 no.4
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    • pp.396-398
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    • 2012
  • Pink mold rot of tomato occurred very severly on tomato farm in Paju, Gyounggi Province, Korea, in July 2005. The infected fruits were dropped and abandoned and the symptoms were similar to calcium deficient tomatoes. But symptom was a slight water-soaked area on or near the blossom end of the fruit and firm greyish-brown lesions were most found at the blossom-end of affected fruits. lesions caused by the pink mold fungus possess a water soaked margin and rise to characteristic orange-pink spores. Also when the diseased tomato was cut, the inside of tomatoes showed completely rotted with pink mold. The causal fungus was identified as Trichothecium roseum based on mycological characteristics. This is the first report of T. roseum infecting greenhouse tomatoes in Korea.

Pythium spp. Isolated from Turfgrasses at Golf Courses in Korea (우리나라 골프장 잔디에서 분리한 Pythium spp.)

  • Kim, Jin-Won;Park, Eun-Woo
    • The Korean Journal of Mycology
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    • v.25 no.4 s.83
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    • pp.276-290
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    • 1997
  • Eleven species of Pythium were identified from 125 isolates collected from leaf blight lesions on creeping bentgrass (Agrostis palustris Huds.), Kentucky bluegrass (Poa pratensis L.), and zoysiagrasses (Zoysia japonica Steud., and Z. matrella (L.) Merr.) at 35 golf courses in Korea in $1990{\sim}1996$. The identified species included P. aphanidermatum, P. arrhenomanes, P. catenulatum, P. graminicola, P. myriotylum, P. oligandrum, P. periplocum, P. rostratum, P. torulosum, P. ultimum var. ultimum, and P. vanterpoolii. Mycological characteristics of sporangia, oogonia, antheridia, and oospores observed on the sucrose-asparagine bentgrass leaf culture medium were described for each species. Of the species, P. arrhenomanes, P. catenulatum, P. gmminicola, P. oligandrum, P. periplocum, P. rostratum, P. torulosum and P. vanterpoolii were reported for the first time in Korea. P. myriotylum, P. rostratum, P. torulosum and P. vanterpoolii showed characteristic colony patterns on the potato-carrot agar medium, which can be used as criteria for species identification of Pythium.

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A Study of Antifungal Activity with Polygonum aviculare Linne (편축 추출물(抽出物)의 항진균작용(抗眞菌作用)에 관한 연구(硏究))

  • Kim, Hong-Sik;Cho, Kwan-Hyun
    • The Korean Journal of Mycology
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    • v.8 no.1
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    • pp.1-5
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    • 1980
  • This study was undertaken to investigate the activity or the water extract of Polygonum aviculare Linne in vitro. Some of the purely isolated strains or dermatophytes were inoculated on Sabouraud's dextrose agar medium containing different concentrations of the Polygonum extracts and their growth was observed for about two weeks at room temperature. Then we measured the sizes of fungal colony grown in various conditions and compared them with those of Sabouraud's medium as control to determine fungistatic effectiveness of the extract. For additional study, slide cultures on Sabouraud's dextrose medium and Sabouraud's media containing 3ml/10ml extract were performed with Epidermophyton floccosum to observe the growth of hyphae, sporulation and other mycological findings. 1. The growth of Epidermo-phyton floccosum was completely inhibited in the media containing 3ml/l0ml Polygonum extract. 2. Trichophyton rubrum, Trichophyton mentagrophytes and microsporum canis were completely inhibited in several strains of each specimen and a moderate inhibitory effect was observed in all of another strains in the media containing the 3ml/10ml extract. 3. In the slide culture of Epidormophyton floccosum the hypha was thin and more desiccated. The characteristic macroco-nidia formation was not observed on the media containing 3ml/10ml Polygonum extract as compared with those findings of Sabouraud's dextrose control medium.

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Characteristics of Phytophthora capsici Causing Pepper Phytophthora Blight Resistant to Metalaxyl (Metalaxyl에 대한 저항성 고추 역병균의 특성)

  • Lee, Soo-Min;Shin, Jin-Ho;Kim, Sun-Bo;Kim, Heung-Tae
    • The Korean Journal of Pesticide Science
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    • v.13 no.4
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    • pp.283-289
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    • 2009
  • Isolation frequency of resistant isolates of Phytophthora capsici to metalaxyl was reported to be 38.9% through the resistance monitoring for metalaxyl in P. capsici causing pepper Phytophthora blight. Metalaxyl was very effective to mycelium growth, while not to zoosporangium germination and zoospore release. $EC_{50}$ values of metalaxyl in the inhibition of mycelium growth were 0.204, 0.151, 0.379, and $0.215\;{\mu}g\;mL^{-1}$ against each isolate sensitive to the fungicide as P. capsici 06-119, 06-143, P08-7, and P08-31, respectively, whilst those were 5.242, 5.724, 6.621, and $5.377\;{\mu}g\;mL^{-1}$ in P. capsici 06-125, 06-155, P08-50, and P08-60. For the field fitness, several factors, which were mycelium growth, zoosporangium germination, zoospore release, virulence to pepper plants, and the zoosporangium and the oospore production, were investigated with 4 sensitive isolates and 4 resistant isolates. Between 2 groups differentiated by the sensitivity of metalaxyl, there was no significance in mycelium growth, zoosporangium germination, zoospore release, and virulence to pepper plants. However, the zoosporangium and the oospore production in each resistant isolate, which were related to survival of P. capsici in fields, were superior to those of sensitive isolates. Based on results of this study, it was suggested that the increase of the percentage of resistant isolates to metalaxyl resulted from the high capacities of the zoosporangium and the oospore production.

Isolation and Identification of Bipolaris coicis, Causing Leaf Blight of Job's Tears (율무 잎마름병을 일으키는 Bipolaris coicis의 분리 및 동정)

  • Kim, Sung-Kee;Kim, Ki-Woo;Hong, Soon-Sung;Park, Eun-Woo;Yang, Jang-Souck;Kim, Yun-Jeong
    • The Korean Journal of Mycology
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    • v.25 no.4 s.83
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    • pp.291-296
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    • 1997
  • Diseased leaves of Job's tears were collected at six locations in Korea to isolate and identify the pathogen causing leaf blight. Conidia were brown, fusoid, and slightly curved. They were $25{\sim}46{\times}10{\sim}15\;{\mu}m$ in size, and had mostly 4 septa which were characteristic of pseudosepta. A few conidia had slightly protruding hila, whereas most had no hilum. Conidial germ tubes were produced mostly from both polar end cells (bipolar), and progressed in semiaxial direction. When seedlings of Job's tears were inoculated with conidia, long, spindle-shaped, and brown lesions were produced on leaves, and entire leaves became blighted 15 days later. Based on the aggressiveness in pathogenicity, the isolates could be divided into two groups. Considering the mycological characteristics and pathogenicity of the fungus, the pathogen causing leaf blight of Job's tears was determined to be Bipolaris coicis (Nisikado) Shoemaker.

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