• 농업과학연구
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• 제40권1호
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• pp.13-18
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• 2013

Effect of electric pulse stimulation was tested on the fruit body formation of valuable mushroom Tricholoma matsutake in the field of natural habitat of this mushroom. After applying the electric stimulator to the specific area of pine forest we found that the treatment especially stimulated the fructification of T. matsutake. And the most valuable findings of our study was that only our treatment plots showed fruit body formation whereas the control plots and the whole natural habitats of our study area showed zero production of this mushroom during the same time. From the point of view of mushroom production, our experiment shows that fruit body production can be upgraded by using pulsed power as an electrical stimulation in the field of the natural habitat of this mushroom. These findings from our experiment confirm the effectiveness of the significance of pulsed power technology for the improvement of T. matsutake fruit body production in the natural habit of this mushroom.

• 한국식품조리과학회지
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• 제10권3호
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• pp.291-295
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• 1994

The main purpose of this research is to survey mushroom uses by housewives in Taegu metropolitan region to provide basic knowledge for the development of mushroom cooking methods. We have done statistical analyses of 472 questionnaires on mushroom uses. We found the following results: many respondents regarded mushrooms as one of natural, or health foods. The most well-known mushrooms turned out to be Pleurotus ostreatus, Dried Lentinus edodes, Ganoderma lucidum, Agaricus bisporus and Tricholoma matsutake in the order. The higher in income and year of education, the more species of mushroom are known to respondents. Anticancer effect of mushrooms are known very well and 56.4% of respondents eat Pleurotus edodes often. Preference between mushrooms and their characteristics have been turned out to be significantly different. Preferred characteristics are taste, texture and aroma: respondents preferred texture of Pleurotus ostreatus, and taste and aroma of Tricholoma matsutake. Main purchasing place for mushrooms turned out to be market. Majorproblems of mushroom uses are price, development of cooking methods, storage and preservation of mushrooms.

• 한국버섯학회지
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• 제20권4호
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• pp.193-198
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• 2022

The biological efficiencies of promising Pleurotus spp. were evaluated. Pleurotus ostreatus, Pleurotus tuberregium,and Pleurotus sajor-cajuwere investigated at temperatures of 22℃, 20℃, and 18℃ to determine their ability to adapt to temperatures that are likely to be found in subtropical regions. The experiment was conducted using a substrate comprising Popular sawdust 60% + cotton seed 20% + beet pulp 20% under a relative humidity of 65%. The results indicated that there were significant differences in terms of biological efficiency between the species: P. tuber regium 128.84% at 22℃, P. ostreatus 108.41% at 22℃, and P. sajor-caju is 80% at 20℃. The common temperatures at which all species showed the highest biological efficiencies were 22℃ and 20℃. Therefore, the production of the evaluated species was equal to or surpassed biological efficiencies reported in tropical environments, thereby demonstrating a potential opportunity to adapt to tropical environments without compromising quality standards.

• Mycobiology
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• 제47권3호
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• pp.350-354
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• 2019

In the post-genomic era, gene function analysis has attracted much attention. Transformation is often needed to investigate gene function. In this study, an easy, rapid, reliable, and cost-effective colony polymerase chain reaction (PCR) method for screening mushroom transformants was developed: picking up a suitable amount of transformant's tissue ($1-10{\mu}g$) to $20{\mu}l$ 0.25% Lywallzyme solution, and vortexing for 10 s followed by incubation at $34^{\circ}C$ for 15 min. Finally, $2{\mu}l$ of the suspension was used as templates to perform PCR and single target bands were successfully amplified from respective transformants of Tremella fuciformis, Pleurotus ostreatus, and Pleurotus tuber-regium. This procedure could be widely employed for screening transformants in mushroom transformation experiments.

• Journal of Animal Science and Technology
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• 제55권4호
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• pp.273-280
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• 2013

This study was conducted to investigate the effects of fermented king oyster mushroom by-products diet (FMBD) on the growth performance, blood characteristics, and carcass traits of fattening pigs and its economics. The fermented diet mainly contained 40.0% king oyster mushroom (Pleurotus eryngii) by-products, 20.0% corn, 28.0% soybean meal, 0.1% supplemental probiotics and 0.08% cellulase. The mixed ingredients were fermented for 1 d at $37^{\circ}C$ followed by 9 d at room temperature, after which they were dried at $45^{\circ}C$ for 3 d. Pigs (n=96) were divided into eight heads per pen, four diet treatments and three replications. The basal diet (C) was substituted with 20% (T1), 50% (T2) and 80% (T3) FMBD. The concentration of crude protein (CP) was significantly higher (p<0.05) at the end of the fermentation period. The average daily gain (ADG) and feed efficiency were significantly lower (p<0.05) in T3 than C. Additionally, carcass grade was significantly better (p<0.05) in all treatments than C and the ratio of high carcass grade was higher. Although substitution of FMBD decreased growth performance, it improved carcass grade and decreased the feed cost of fattening pigs. Therefore, it was expected that the increase in the utilization ratio of FMBD will reduce the cost of animal production.

• 방사선산업학회지
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• 제5권2호
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• pp.169-173
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• 2011

This study investigated the effect of gamma irradiation on immune enhancing activity of Chaga mushroom extract (CME). CME was prepared by hot water extraction at $70^{\circ}C$ for 4 hours and lyophilized. Lyophilized CME powder was dissolved with deionized water at $10mg\;ml^{-1}$ and then irradiated at the doses of 10, 30 and 50 kGy by cobalt 60 gamma irradiator. The gamma-irradiated and non-irradiated CME were treated into the splenocyte separated from mouse. Cell proliferation and cytokine production of the immune cells were increased by gamma-irradiated CME and these increases were more prominent when CME was irradiated at higher doses. Therefore, it is considered that gamma irradiation can be an effective method for improvement of the immunomodulating activity Chaga mushroom extract.

• Animal Bioscience
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• 제34권10호
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• pp.1695-1704
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• 2021

Objective: The aim of this work was to assess the effect of ergothioneine (ESH)-enriched mushroom extract on oxidative stability, volatile compounds, and sensory quality of emulsified sausage. Methods: The ESH content was determined by high performance liquid chromatography. The antioxidant activity of Flammulina velutipes (F. velutipes) extract was determined through radical-scavenging activity of 1,1 diphenyl-2-picryl-hydrazyl, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) and hydroxyl radicals. Four different groups of emulsified sausage were manufactured: control, no antioxidants; BHA, 0.01% butylated hydroxyanisole; EEME, 0.8% ESH-enriched mushroom (F. velutipes) extract; AE, 0.012% authentic ESH, after storage for 14 days (at 4℃), the quality of sausage including oxidative stability (2-thiobarbituric acid reactive substances and protein carbonyls content), volatile compounds and sensory quality were studied. Results: It was demonstrated that adding ESH-enriched F. velutipes extract to sausage could effectively prevent lipid and protein oxidation, and its efficacy was equivalent with 0.01% BHA. During meat processing, the ESH mainly contributed to the antioxidative activity of F. velutipes extract. The flavor and sensory attributes of emulsified sausage were improved through adding ESH-enriched F. velutipes extract. Conclusion: Accordingly, the extract of F. velutipes contained high-level of ESH and could be a good antioxidant candidate for processed meat production.

• 한국버섯학회지
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• 제15권4호
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• pp.249-253
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• 2017

Cellulase와 xylanase 분비능이 우수한 세균을 분리하기 위하여 부여군 석성면 지역의 양송이 재배농장으로부터 수확후배지를 수집하였다. 양송이 수확후배지로부터 12종의 균주를 분리하였으며 이 중 cellulase와 xylanase 활성이 가장 우수한 균주 NO12를 최종 선발하였다. 분리균 NO12의 생리적 생화학적 특성은 Bacillus ID kit와 MicroLog system을 이용하여 조사하였으며 분리균 NO12는 Bacillus subtilis와 유사한 특징을 나타내었다. 분리균 NO12의 16S rDNA 염기서열도 B. subtilis와 99.2%의 상동성을 나타내었다. 이와 같은 결과를 종합하여 분리균 NO12는 B. subtilis NO12로 동정되었다. 분리균이 분비하는 cellulase와 xylanase 활성은 분리균이 증식함에 따라 대수증식기 중반부터 급격히 증가하였고 정지기에 진입하면 효소활성이 더 이상 증가하지 않는 것으로 나타났으며 xylanase 활성은 대수증식기 초기부터 지속적으로 증가하여 대수증식기 중반에 최대활성을 나타내었다.

• Journal of Animal Science and Technology
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• 제49권1호
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• pp.79-88
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• 2007

본 연구에서는 폐배지의 동물 사료로의 효율적 이용을 위하여 국내에서 발생되는 폐배지의 최소한의 발생량을 버섯종류와 버섯재배 방식에 따라 산출하고, 느타리, 새송이, 팽이 버섯폐배지의 물리화학적 특성을 버섯종류별과 재배방식별로 평가하고자 실시하였다. 2004년도 우리나라 폐배지 총 발생량은 1,670,182M/T이었다. 사료로의 이용가능성이 상대적으로 높을 것으로 예상되는 새송이버섯, 팽이버섯, 느타리버섯 폐배지는 전체 발생량의 58.2%인 972,141 M/T이 발생되었으며, 이중 재배방식별로는 병재배에 의해 67%, 균상재배에 의해 20%, 봉지재배에 의해 13%가 발생되었으며, 버섯 종류별로는 느타리버섯 폐배지가 46%, 팽이버섯 폐배지가 32%, 새송이버섯 폐배지가 22% 발생되었다. 버섯 재배방식별 폐배지의 물리화학적 특성에 있어서 균상재배 폐배지는 병재배와 봉지재배 폐배지 보다 NDF 함량(65.2%)이 낮고, NFC 함량(12.7%)이 높은 특징이 있어서(P<0.05), 사료적 가치가 상대적으로 높을 것으로 사료되었다. 폐배지의 화학적 성분 변이도는 병 또는 봉지재배 방식보다 균상재배 방식에서 높은 편이었으며, 전반적으로 섬유소(NDF) 함량의 변이도는 예상보다 낮은 편이었다. 결과적으로 폐배지를 동물 사료로 이용 시 버섯종류에 따라 분류하는 것보다는 재배방식에 따라 분류하여 사용하는 것이 바람직하였으며, 사료영양적 측면에서 가치가 높은 균상재배 폐배지의 우선적 이용이 권장된다.

• 한국포장학회지
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• 제20권3호
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• pp.71-76
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• 2014

농가로부터 판매처까지의 유통단계에서 변화하는 온도 조건에 노출된 생표고버섯의 저장성향상을 확보하기 위해 개별포장과 2차 포장을 결합시킨 마스터 포장 시스템을 적용하였다. 온도가 저온으로 유지되는 수송과 저장 단계에서는 1차와 2차 포장의 결합 형태로 유통되고, 온도가 높은 판매단계에서는 외부 2차 포장을 해체한 후 1차 포장 조건에서 적절한 가스농도를 확보할 수 있는 개념으로 설계하고, 2차 마스터 포장 내에는 $Ca(OH)_2$의 이산화탄소 흡수제를 포함시켰다. 유통흐름 중에서 포장의 온습도와 기체조성을 측정하고, 마지막 단계인 판매처에서 포장을 개봉하여 버섯의 품질을 측정하였다. 공기조성을 유지하는 관행적인 통기성 천공 포장을 대조구로 함께 유통흐름을 거치게 하여 저장 후 품질을 비교하였다. 초기의 수송 및 저장 단계에서 적절한 저온조건이 유지되지 못한 것으로 나타났으나, 유통의 전체 과정에서 버섯 주위에 산소 농도 0.4~4.2%, 이산화탄소 농도 0.7~1.7%로 품질보존에 도움이 되는 기체조성이 유지되었다. 포장 전 예건처리가 버섯의 품질보존에 효과가 있는 반면에, 마스터 포장 시스템의 긍정적 효과는 비예건 버섯에 뚜렷이 나타났다. 보다 효과적인 버섯 품질보존을 위해서 예건 처리 및 온도관리와 함께 조화된 마스터 포장 시스템의 설계가 추가적으로 이루지고 검토될 필요가 있는 것으로 생각된다.