• The Korean Journal of Mycology
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• v.32 no.2
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• pp.145-147
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• 2004

The ethyl acetate extracts from the liquid cultures of Coriolus versicolor, Phellinus linteus, and Hericium erinaceus showed significant antibacterial activities against Escherichia coli K88, E. coli K99, E. coli 987P, and Salmonella typhimurium 14058 causing bacterial diarrhea in Korean house pigs and chicken. Of these extracts, Coriolus versicolor extract showed the highest antibacterial activity. In addition, these extracts also showed significant growth inhibition against Staphylococcus aureus CARM3230 and E. coli CARM1381 which are known as kanamycin and ampicillin-resistant strains. These results showed that the mushroom extracts could be developed as a livestock feed additives that can replace commercial antibiotics, and also could be good resources for the development of a new antibacterial agent.

• The Plant Pathology Journal
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• v.27 no.2
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• pp.138-147
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• 2011

Xylogone ganodermophthora, an ascomycetous fungus, is known to cause yellow rot in the cultivated mushroom Ganoderma lucidum. In this study, we investigated the dissemination of this fungal pathogen in G. lucidum grown in cultivation houses. To determine the role of ascospores produced by X. ganodermophthora in disease development, we constructed a green fluorescent protein-labeled transgenic strain. This X. ganodermophthora strain produced a number of ascomata in the tissues of oak logs on which G. lucidum had been grown and on the mushroom fruit bodies. However, the ascospores released from the ascomata were not able to germinate on water agar or potato dextrose agar. Moreover, less than 0.1% of the ascospores showed green fluorescence, indicating that most ascospores of X. ganodermophthora were not viable. To determine the manner in which X. ganodermophthora disseminates, diseased oak logs were either buried in isolated soil beds as soil-borne inocula or placed around soil beds as air-borne inocula. In addition, culture bottles in which G. lucidum mycelia had been grown were placed on each floor of a five-floor shelf near X. ganodermophthora inocula. One year after cultivation, yellow rot occurred in almost all of the oak logs in the soil beds, including those in beds without soil-borne inocula. In contrast, none of the G. lucidum in the culture bottles was infected, suggesting that dissemination of X. ganodermophthora can occur via the cultivation soil.

• Journal of Mushroom
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• v.14 no.4
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• pp.191-196
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• 2016

This study was conducted to investigate optimum conditions for mass production of ntagonistic microbes Alcaligenes sp. HC12. Alcaligenes sp. HC12 had a potent biological control agent to control browning disease caused by Pseudomonas agarici. Alcaligenes sp. HC12 markedly showed the antagonistic activity against Pseudomonas agarici, the most destructive pathogen of cultivated mushrooms. To define the optimum conditions for the mass production of the Alcaligenes sp. HC12, we have investigated optimum culture conditions and effects of various nutrient source on the bacterial growth. The optimum initial pH and temperature were determined as pH 9.0 and $30^{\circ}$, respectively. The optimal concentration of medium elements for the growth of pathogen inhibitor bacterium(Alcaligenes sp. HC12) was determined as follows: 0.5% dextrine, 1.5% yest extract, 1.0% $NaNO_3$, 0.5% $KH_2PO_4$, and 1.5% asparagine.

• Mycobiology
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• v.48 no.5
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• pp.427-430
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• 2020

Ganoderma lingzhi is a well-known source of natural fungal medicines which has been given for the treatment of several diseases. China is one of the major commercial producers of Ganoderma mushroom worldwide. However, with the expansion of the commercial cultivation, the occurrence of the fungal diseases on G. lingzhi has also been increased. The green mold disease symptoms were observed in the cultivation base of G. lingzhi in Zuojia Town, Jilin City, Jilin Province, China, causing the basidiomes to be rotten and withered, and the green mycelium layer generated gradually. The pathogenicity tests showed the same symptoms as appeared naturally in Zuojia mushroom base. Morphology characters revealed conidia green, ellipsoid, globose, 2.56-4.83 × 2.09-4.22 ㎛, length-width ratio was 1.1-1.2 (n = 10). Conidiophores trichoderma-like, often asymmetry, branches solitary, paired or in whorls of 3 phialides formed solitary, paired or in whorl, variable in shape, lageniform, sometimes ampulliform or subulate. While using molecular methodology, comparing with the sequences of Trichoderma hengshanicum from GenBank, the analyzed sequence showed 97.32% homology with the RPB2 sequences, 100% with the TEF1-a sequences. A fungus isolated from the diseased tissues was identified based on morphology and molecular studies as T. hengshanicum. This is the first report of T. hengshanicum causing the green mold disease of G. lingzhi in China.

• Journal of Mushroom
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• v.13 no.4
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• pp.301-304
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• 2015

This study was carried out to investigate the optimum rate of substrate composition in bottle cultivation of Grifola frondosa and had three rates of substrate composition of 67:11:22(T1), 68:15:17(T2) and 74:14:12(T3) as mixing rate of weight of dried oak sawdust, dried corn husk and dried bean-curd refuse. The rate of primordia formation of T3 was 65.8% which was lowest among all treatments. Contraction rate of disease of T1 was 9.8% which was highest among all treatments. Harvesting rate of T2 was 70.5% which was highest among all treatments. Fruit body weights per bottle of T1 and T2 were 85.5 g, 83.3 g respectively and there was not significant difference between those. Yield per 10,000 bottles of T2 was 587 kg and was 7%, 28% higher than those of T1 and T3, respectively. As a result, the rate of substrate composition of 68:15:17(T2) as mixing rate of weight of dried oak sawdust, dried corn husk and dried bean-curd refuse was appeared as optimum rate of substrate composition in bottle cultivation of Grifola frondosa..

• Journal of Pharmaceutical Investigation
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• v.19 no.3
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• pp.137-143
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• 1989

This study was attempted to investigate the effect of Tricholoma matsutake on experimentally induced hyperlipemia in rats. The levels of cholesterol, triglyceride and phospholipid were measured. Diagnostic attention has been paid to the cholesterol concentration associated with high density lipoprotein (HDL), which appears to be inversely related to the incidence of coronary artery disease. For these reasons, determination of HDL-cholesterol has significant meaning. Total cholesterol was determined by Abell-Kindall method, and to measure HDL-cholesterol, serum low density lipoproteins (LDL) were first selectively precipitated by HDL-precipitating reagent. The results obtained were as follows : 1) The level of total cholesterol in serum of hyperlipemic rats was decreased by oral administration of Tricholoma matsutake extract. 2) The level of HDL-cholesterol was increased by the mushroom extract. 3) The level of triglyceride was significantly decreased by the mushroom extract. 4) The level of phospholipid was slightly decreased by the mushroom extract.

• Applied Biological Chemistry
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• v.43 no.3
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• pp.190-195
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• 2000

Tolaasin is a peptide toxin produced by Pseudomonas tolaasii and causes a brown blotch disease forming brown, slightly sunken spots and blotches on the cultivated mushrooms. It is a lipodepsipeptide consisting of 18 amino acids and its molecular mass is 1,985 Da. It forms a pore in plasma membranes, resulting in the disruption of membranes of fungal, bacterial, plant, and animal cells as well as mushroom tissue. In order to measure the toxicity of tolaasin, erythrocytes of blood were used to evaluate the tolaasin-induced hemolysis. Hemolytic activity of tolaasin was measured by observing the absorbance change either at 420 nm, representing the release of hemoglobins from red blood cells(RBCs), or at 600 nm, representing the density of residual cells. The hemolytic activity of culture-extract of P. tolaasii increased at early-stationary phase of growth and was maximal at late stationary phase. The hemolytic activity of tolaasin appeared high in the RBCs of dog and rat. The RBCs of rabbit and hen were less susceptible to tolaasin. The effects of various cations were also measured. $Cd^{2+}$ and $La^{3+}$. as well as $Zn^{2+}$ appeared inhibitory to the tolaasin-induced hemolysis. The effects of various anions on tolaasin-induced hemolysis were measured and carbonate showed the greatest inhibition to the hemolysis. However, phosphate stimulated the tolaasin-induced hemolysis and no effects were observed by chloride and nitrate.

• Korean Journal of Environmental Biology
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• v.18 no.4
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• pp.441-446
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• 2000

Trichoderma harzianum is an aggressive causal agent of green mold disease on mushroom cultivation. Some bacterial strains isolated, from oyster mushroom compost in Wonju, were found to have in vitro antifungal activity against Trichoderma harzianum ATCC 6385, 6504, and our isolates Trichoderma spp. Y and G. Further in vitro antifungal studies on several strains of phytopathogenic fungi showed that all of 12 phytopathogenic fungal strains were significantly inhibited by the isolated antifungal bacteria in Petri dishes. Of these, KATB 99121 showed the broadest inhibiting effect and displayed as negative coagulase, negative sulfide production and rod shape. KATB 99121 was resistant to ampicillin, chlorampenicol, and kanamycin. Identification of isolates was determined by Biolog GN system, and KATB 99121 was identified as Photobacterium logei because of 96 probability, 0.65 similarity, and 4.97 disturbance. With electron microscopy, thin section of KATB 99121 strain revealed typical rod-like shaped cell (0.6-0.8${\mu}{\textrm}{m}$$\times$1.5-2.0${\mu}{\textrm}{m}$) with prokaryotic structure and organization.

• Journal of Mushroom
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• v.2 no.2
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• pp.102-108
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• 2004

The mushroom production is rapidly growing worldwide and mushroom present a great potential in agricultural promotion in Korea. Many researches have been done on the cultivation, disease control and breeding of mushrooms over last 20 years. The researches, on the other hand, on the utilization and processing of mushrooms and comparatively poor. A survey on consumer behavior of Korean, Chinese and Japanese consumers on mushrooms procurement and consumption was carried out. The main purchasing place for mushrooms by Korean and Japanese consumers was mart while the conventional market was the main purchasing place for Chinese consumers. Chinese and Japanese consumers had better recognition on processed mushroom products compared to Korean consumers. Chinese and Japanese consumers bought pickled mushrooms while Korean consumers were experienced in tea products made of mushrooms. The most popular method for cooking mushrooms by all the three country's consumers is saute.

• Journal of Mushroom
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• v.17 no.4
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• pp.179-184
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• 2019

This study was conducted to investigate the diversity of culture-dependent fungal species in the sawdust media of Lentinula edodes. A total of 405 fungi were isolated from the specimens and identified to belong to 24 genera and 42 species. Among the identified 42 species of fungi, 26.2% belonged to Penicillium sp., 9.5% belonged to Trichoderma sp., and 64.3% belonged to others. Especially, Trichoderma harzianum, which is a causal agent of fungal disease in mushroom, was found on all the farms, and showed the highest frequency among the identified fungi. Community analysis showed that the fungal diversity patterns of the samples were similar to each farm and many kinds of fungi existed in the sawdust media at high levels. These results showed that the management of internal environments would be required for the stable cultivation of Lentinula edodes.