Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
권호 표지
DOI QR코드

DOI QR Code

Optimum cultivation conditions for mass production of antagonistic bacterium Alcaligenes sp. HC12 effective in antagonistic of browning disease caused by Pseudomonas agarici

버섯 세균성회색무늬병균(Pseudomonas agarici)에 대한 길항 세균 Alcaligenes sp. HC12의 대량배양을 위한 최적 배양조건

  • 이찬중 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 문지원 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 정종천 (농촌진흥청 국립원예특작과학원 버섯과)
  • Received : 2016.12.06
  • Accepted : 2016.12.15
  • Published : 2016.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate optimum conditions for mass production of ntagonistic microbes Alcaligenes sp. HC12. Alcaligenes sp. HC12 had a potent biological control agent to control browning disease caused by Pseudomonas agarici. Alcaligenes sp. HC12 markedly showed the antagonistic activity against Pseudomonas agarici, the most destructive pathogen of cultivated mushrooms. To define the optimum conditions for the mass production of the Alcaligenes sp. HC12, we have investigated optimum culture conditions and effects of various nutrient source on the bacterial growth. The optimum initial pH and temperature were determined as pH 9.0 and $30^{\circ}$, respectively. The optimal concentration of medium elements for the growth of pathogen inhibitor bacterium(Alcaligenes sp. HC12) was determined as follows: 0.5% dextrine, 1.5% yest extract, 1.0% $NaNO_3$, 0.5% $KH_2PO_4$, and 1.5% asparagine.

버섯 세균갈성색무늬병원균인 Pseudomonas agarici에 대한 길항미생물로 보고된 Alcaligenes sp. HC12 균주의 배양적 특성과 대량 배양을 위한 최적배양 조건을 설정하였다. HC12균주의 생육온도는 $20{\sim}40^{\circ}C$, pH는 6.0 이상에서 왕성한 생육을 보였다. 대량배양을 위한 효율적인 영양원 선발을 위하여 기본배지에 탄소원 fructose 등 18종, 무기질소원 $NH_4Cl$ 등 6종, 유기질소원 peptone 등 6종 그리고 아미노산 asparagine 등 11종을 각각 1%씩 첨가하였고, 무기염류 13종을 첨가하여 각각에 대한 생육에 미치는 영향을 조사하였다. 또한 선발된 각각의 영양성분들에 대한 최적 농도를 조사하기 위하여 각각의 성분을 최소 0.1%에서 최대 4.0%까지 배지에 첨가하여 배양 후 생육정도를 조사하였다. 그 결과, 대량배양을 위한 생육최적조건은 온도 $30^{\circ}C$, pH 9, 탄소원 0.5% dextrine, 유기질소원 1.5% yeast extract, 무기질소원 1.0% $NaNO_3$, 아미노산 1.5% asparagine 그리고 무기염류는 0.5% $KH_2PO_4$에서 왕성한 생육을 보였다.

Keywords

References

  1. Bessette AE, Kerrigan RW, Jordan DC. 1985. Yellow blotch of Pleurotus ostreatus. Appl Environ Microbiol. 50:1535-7.
  2. Cutri SS, Macauley BJ, Roberts WP. 1984. Characteristics of pathogenic non-fluorescent (smooth) and non-pathogenic fluorescent (rough) forms of Pseudomonas tolaasii and Pseudomonas 'gingeri'. J Appl Bacteriol. 57:91-298.
  3. Geels FP, Hesen LP, Van Griensven LJ. 1994. Brown discoloration of mushrooms caused by Pseudomonas agarici. J Phytopathol, 140:249-59. https://doi.org/10.1111/j.1439-0434.1994.tb04814.x
  4. Lee CJ, Yoo YM, Han JY, Jhune CS, Cheong JC, Moon JW, Suh JS, Han HS, Cha JS. 2014. Isolation of the bacterium Pseudomonas azotoformans HC5 effective in antagonistic of brown blotch disease caused by Pseudomonas tolaasii. Kor J Mycol 42:219-224. https://doi.org/10.4489/KJM.2014.42.3.219
  5. Lee CJ, Yoo YM, Han JY, Jhune CS, Cheong JC, Moon JW, Suh JS, Han HS, Cha JS. 2013. Isolation of the bacterium Pseudomonas sp. HC1 effective in inactivation of tolaasin produced by Pseudomonas tolaasii. Kor J Mycol. 41: 248-254. https://doi.org/10.4489/KJM.2013.41.4.248
  6. Lee CJ, Moon JW, Cheong JC, Kong WS. 2016. Effective in antagonistic on browning disease caused by Pseudomonas agarici of the bacterium Alcaligenes sp. HC12. Kor J Mycol. 44:171-175.
  7. Lee HI, Cha JS. 1998. Cloning of a DNA fragment specific to Pseudomonas tolaasii causing bacterial brown blotch disease of oyster mushroom (Pleurotus ostreatus). Korean J Plant Pathol. 14:177-83.
  8. Liao YM, Tu CC, Jeng JJ. 1980. Control of bacterial blotch of mushroom. Taiwan Mushrooms. 4:34-41.
  9. Nair NG, Fahy PC. 1972. Bacteria antagonistic to Pseudomonas tolaasii and their control of brown blotch of the cultivated mushroom Agaricus bisporus. J Appl Bacteriol. 35:439-42. https://doi.org/10.1111/j.1365-2672.1972.tb03720.x
  10. Nutkins JC, Mortishire-Smith RJ, Packman LC, Brodey CL, Rainey PB, Johnstone K, Williams DH. 1991. Structure determination of tolaasin, an extracellular lipodepsipeptide produced by the mushroom pathogen Pseudomonas tolaasii Paine. J Am Chem Soc. 113:2621-7. https://doi.org/10.1021/ja00007a040
  11. Olivier JM, Guillaumes J, Martin D. 1978. Study of a bacterial disease of mushroom caps. In: Proceedings of 4th International Conference Plant Pathogenic Bacteria.; 1978 Aug 27-Sep 2; Angers, France. Paris: INRA. p. 903-16.
  12. Paine SG. 1919. Studies in bacteriosis II. A brown blotch disease of cultivated mushrooms. Ann Appl biol. 5:206-219. https://doi.org/10.1111/j.1744-7348.1919.tb05291.x
  13. Park BS, Cho NC, Chun UH. 1992. Identification of Pseudomonas fluorescens antagonistic to Pseudomonas tolaasii and its cultivation. Kor J Biotechnol Bioeng. 7:296-301.
  14. Reasoner DJ, Geldreich EE. 1985. "A new medium for the enumeration and subculture of bacteria from potable water," Appl Environ Microbiol. 49:1-7.
  15. Scherwinski K, Grosch R, Berg G. 2008. Effect of bacterial antagonists on lettuce: active biocontrol of Rhizoctonia solani and negligible, short-term effects on nontarget microorganisms. FEMS Microbiol Eco. 64:106-16. https://doi.org/10.1111/j.1574-6941.2007.00421.x
  16. Stainer RY, Palleroni NJ, Doudoroff M. 1966. The aerobic pseudomonads: a taxonomic study. J general Microbiol 43:159-271. https://doi.org/10.1099/00221287-43-2-159
  17. Tolaas AG. 1915. A Bacterial disease of cultivated mushrooms. Phytopathology 5: 51-54
  18. Wells JM, Sapers GM, Fett WF, Butterfield JE, Jones JB, Bouzar H, Miller FC. 1996. Postharvest discolorization of the cultivated mushroom Agaricus bisporus caused by Pseudomonas tolaasii, P. 'reactans', and P. 'gingeri'. Phytopathology 86: 1098-1104. https://doi.org/10.1094/Phyto-86-1098
  19. Wong WC, Fletche, JT, Unsworth BA, Preece TF. 1982. A note on ginger blotch, a new bacterial disease of the cultivated mushroom, Agaricus bisporus. J Appl Bacteriol. 52:43-48. https://doi.org/10.1111/j.1365-2672.1982.tb04371.x