• Mycobiology
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• v.32 no.3
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• pp.115-118
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• 2004

A composting technique was assessed to enhance efficiency in oyster mushroom substrate. Poplar sawdusts and by-product of winter mushroom added by 10% of rice bran were composted outdoors at $20^{\circ}C$ to $28^{\circ}C$ for 12 days. The duration of fermentation was shortened 3 days in fermented sawdust plot. The yield obtained from waste cotton substrate with fermented poplar sawdust was 742 g, whereas the yield from control 663 g. In addition, the substrate with by-product of winter mushroom was the most effective to composting. It was useful to apply the fermented sawdust and by-product to waste cotton for compost and sporophore production of oyster mushroom.

• Journal of Mushroom
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• v.15 no.3
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• pp.134-138
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• 2017

The white button mushroom, Agaricus bisporus, is commercially the fifth most important edible mushroom, accounting for the production of 9,732 tons of mushrooms in Korea in 2015. The genus Agaricus has been known for its potential to degrade lignocellulosic materials. Chemical analyses carried out during the cultivation of A. bisporus indicated that the cellulose, hemicellulose, and lignin fractions were changed preferentially for both vegetative growth and sexual reproduction. We screened A. bisporus strains for effective biodegradation through extracellular enzyme activity using cellulase, xylanase, and ligninolytic enzymes. The enzyme biodegradations were conducted as follows: mycelia of collected strains were incubated in 0.5% CMC-MMP (malt-mops-peptone), 0.5 Xylan-MMP, and 0.5% lignin-MMP media for 14 days. Incubated mycelia were stained with 0.2% trypan blue. Eighteen strains were divided into 8 groups based on different extracellular enzyme activity in MMP media. These strains were then incubated in sterilized compost and compost media for 20 days to identify correlations between mycelial growth in compost media and extracellular enzyme activity. In this study, the coefficient of determination was the highest between mycelial growth in compost media and ligninolytic enzyme activity. It is suggested that comparison with ligninolytic enzyme activity of the tested strains is a simple method of screening for rapid mycelial growth in compost to select good mother strains for the breeding of A. bisporus.

• Journal of Mushroom
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• v.16 no.1
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• pp.39-44
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• 2018

We investigated the possibility of utilizing spent mushroom culture-medium as raw material to produce organic compost. Mushroom culture-medium contained 2.69% nitrogen, 41.07% carbon, and 0.99% phosphoric acid. Nitrogen and carbon content in rice bran were 3.08% and 47.34%, respectively. Nitrogen, carbon, and phosphoric acid content in mushroom Pleurotus eryngii were 3.41%, 34.63%, and 1.70%, respectively. Our study aimed to evaluate the degree of decay of compost produced from the substrate, used to culture King Oyster mushrooms by analyzing seed germination indexes in cucumber, radish, lettuce, and Chinese cabbage. Our results showed that compost from spent mushroom culture-medium completed the stabilization process in 4 to 6 weeks, as evaluated by the appropriate organic compost maturity point for the plantation.

• Journal of Mushroom
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• v.15 no.2
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• pp.61-68
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• 2017

This study focused on evaluating the phytoextraction of heavy metals (Co, Pb, and Zn) induced by bioaugmentation of button mushroom compost (BMC) in Helianthus annuus (sunflower). When the potential ability of BMC to solubilize heavy metals was assessed in a batch experiment, the inoculation with BMC could increase more the concentrations of water-soluble Co, Pb, and Cd by 35, 25, and 45% respectively, compared to those of non-inoculated soils. BMC-assisted growth promotion and metal uptake in H. annuus was also evaluated in a pot experiment. In comparison with non-inoculated seedlings, the inoculation led to an increase in the growth of H. annuus by 27, 25, and 28% in Co-, Pb-, and Zn-contaminated soils, respectively. Moreover, enhanced accumulation of Co, Pb, and Zn in the shoot and root systems was observed in inoculated plants, where metal the translocation from root to the above-ground tissues was also found to be enhanced by the BMC. Evidently, these results suggest that the BMC could be effectively employed in enhancing the phytoextraction of Co, Pb, and Zn from contaminated soils.

• Mycobiology
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• v.41 no.4
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• pp.214-220
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• 2013

This study was conducted in order to perform efficient extraction of lignocellulolytic enzymes amylase (EC 3.2.1.1), cellulase (EC 3.2.1.4), laccase (EC 1.10.3.2), and xylanase (EC 3.2.1.8) from spent mushroom compost (SMC) of Pleurotus ostreatus, P. eryngii, and P. cornucopiae. Optimal enzyme recovery was achieved when SMCs were extracted with 50 mM sodium citrate (pH 4.5) buffer at $4^{\circ}C$ for 2 hr. Amylase, cellulase, and xylanase activities showed high values in extracts from P. ostreatus SMC, with 2.97 U/g, 1.67 U/g, and 91.56 U/g, respectively, whereas laccase activity and filter paper degradation ability were highest in extracts from P. eryngii SMC, with values of 9.01 U/g and 0.21 U/g, respectively. Enzymatic activities varied according to the SMCs released from different mushroom farms. The synthetic dyes remazol brilliant blue R and Congo red were decolorized completely by the SMC extract of P. eryngii within 120 min, and the decolorization ability of the extract was comparable to that of 0.3 U of commercial laccase. In addition, laccase activity of the SMC extract from P. eryngii was compared to that of commercial enzymes or its industrial application in decolorization.

• Korean Journal of Agricultural Science
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• v.9 no.1
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• pp.269-274
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• 1982

These studies were conducted to evaluate the bulk pasteurization system in comparison with the conventional mushroom growing technique of and to establish the phase II fermentation method for the system. The results obtained were as follows. 1. From the mushroom compost peak-heated in the bulk pasteurization system higher mushroom yield was obtained than that of the conventional method. 2. The compost fermented in the bulk pasteurization system showed poor mycelial growth and low crop. It was caused by the imperfect blower and its operation, not by ununiform moisture content of the compost and ununiform filling. 3. A bulk pasteurization system which modified the mushroom house was not proper for the fermentation of the mushroom compost and the sporophore yields were lower than the standard pasteurization system. 4. In the bulk pasteurization system, peak-heating time, phase II period and quality of the compost were influenced by the air temperature but its effects were not more significant than those of the conventional method. 5. During phase II in the bulk pasteurization system moisture content of the compost at filling did not affect the fermentation of the compost.

• The Korean Journal of Mycology
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• v.1 no.2
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• pp.1-7
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• 1973

Among the factors which affect the mushroom yield, this investigation was aimed to confim the relationship between nitrogen content of rice straw compost and crop yield, residual ammonia content and yield. In this investigation the nitrogen content in dry weight of compost ranged from 1.10 to 2.06% and the residual ammonia content between 0.01 and 0.8% at spawning according to the sources of organic or inorganic nitrogen and the formulas of compost materials. The results obtained are summarized as follows: 1. The relationship between nitrogen content of rice straw compost and mushroom yield was represented by the formula: y=13.95+0.048$(r=0.68^{**})$. 2. Nitrogen content and mushroom yield of compost supplemented with organic nitrogen sources were increased as compared with control or inorganic treatments. 3. The relationship between the residual ammonia content and mushroom yield is represented by the formula: $y=0.38086-0.011948+0.00012x^2(r=-0.75^{**})$. In order to obtain high cropping yield the residual ammonia content at spawning should be below 0.03% (expressed as nitrogen content). 4. Application of ammonium sulfate increased the nitrogen content of compost slightly, but the residual ammonia content was increased considerably and yield decreased. On the other hand, the residual ammonia content of urea treatment was low and increased mushroom yield remarkably.

• The Korean Journal of Mycology
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• v.27 no.4 s.91
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• pp.289-292
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• 1999

We investigated the isolation method of mushroom infesting pests, Lycoriella mali, Coboldia fuscipes, Histiostoma sp. from mushroom-growing compost. Sugar solution of different densities (0, 10, 20, 30, 40, 50%) was tested to provide a means of seperating mushroom pests from the compost media. Thus, 40% sugar solution was suitable for isolation. The sieve size to entrap the pests was $30{\sim}140$ mesh; Lycoriella mali was trappped mainly $30{\sim}65$ mesh sieve, Coboldia fuscipes was caught mainly $30{\sim}100$ mesh sieve, Histiostoma sp. was trapped mainly $65{\sim}140$ mesh sieve. An isolation procedure was as follows; The infested compost was disintegrated in water and poured onto a set of 16, 30, 80, 140-mesh sieve. The material caught in the sieve is added in 40% sugar solution and then most compost particle were massed at the bottom while the supernatant contains mushroom pests. The upperlayer material was poured into a Seperatory funnel and the sediment at the bottom is drained off. The remaining material are washed off examination dish for study.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.2
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• pp.123-131
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• 2009

This study was carried out to investigate the feasibility for the use of environmental-friendly materials and the effective recycling of spent mushroom compost(SMC) after cultivation of Button Mushroom, Agaricus bisporus. SMC of white button mushroom contained diverse microorganisms including fluorescent Pseudomonas sp., Bacillus sp., Tricoderma sp. and Actinomycetes. These isolates showed the extensive antifungal spectrum against plant pathogen. Among of the isolates, fungal pathogen such as Alternaria brassicicola, Phytophtora melonis, Phytophthora capsici and Colletotichum gloeosporioides strong showed strong antagonistic activity. 45.8% of the isolates were actively colonized on the pepper root and 5.8% showed rhizosphere competent of >$5{\times}10^2cfu\;root^{-1}$. The plant growth promotion ability of the collected isolates were tested in pot experiments using red pepper seedling. Among them, 62.7% showed pepper growth promoting ability and growth of pepper root showed superior to the control. The germination of pepper treated with aqueous extracts of non-harvest SMC completely inhibited at concentration of more than 33%. The sterilization of SMC resulted in higher inhibition of germination and early growth of pepper. These results suggest that spent mushroom compost(SMC) of Button Mushroom may have adequately the feasibility for the use with environmental-friendly materials.

• Journal of Mushroom
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• v.8 no.3
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• pp.122-130
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• 2010

Common mushroom production per area has been decreased and are up to less than 50% of the 1980 production. To determine the main reasons for the decrement, we performed this study. Two main reasons, which are mushroom disease and the low compost quality because of mechanized compost making, were assessed. In mechanized mushroom farms, nitrogen concentration in compost was lower than recommended and total compost quantity was about 100-150 $kg/3.3m^2$, which was also lower than usual. Our study revealed that higher nitrogen concentration (about 1.5%) in compost gave better production. Also, use of large amount of compost appeared to increase the mushroom production, although more insects and disease problems were observed. The relationships between the presences of microorganisms and occurrence of diseases were assessed by monitoring the microorganism densities near the mushroom farms. Higher number of microorganisms were observed near the mushroom farm area, compared to control region, Daechon beach. Most contaminating molds were found in the circulating fans, tunnel and culture room floor. The bacterial isolates were collected from the air in mushroom culture room and killed with 0.005% Benzalkonium solution, indicating treatment of Benzalkonium are the effective methods to sanitize the mushroom culture room.