• Journal of Digital Convergence
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• v.17 no.12
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• pp.435-444
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• 2019

The purpose of this study was to investigate the relationship between sleep duration and muscle strength in Korean adults aged 19 years and older. The cross-sectional analysis was conducted using the 2016 National Health and Nutrition Examination Survey data and Chi square test and multiple regression analysis were used. As a result of the analysis, the grip strength of those with more than weekday average sleep duration of 9 hours was found to be -1.267kg compared with those with weekday average sleep duration of 7 hours. The grip strength of those with more than weekend average sleep duration of 9 hours was found to be -0.879kg compared with those with weekend average sleep duration of 7 hours. In model simultaneously adjusting for both the average weekday and weekend average sleep duration, weekday average sleep duration of 9 hours was found to be -1.034kg compared with those with weekday average sleep duration of 7 hours. Therefore, careful observation will be required in light of the fact that both sleep duration and grip strength can predict future health conditions.

• Journal of Periodontal and Implant Science
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• v.37 no.4
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• pp.859-869
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• 2007

Naked DNA and standard vectors have been previously used for gene delivery. Among these, PEI can efficiently condense DNA and has high intrinsic endosomal activities. The aim of this study is to investigate whether the cationic polycation PEI could increase the transfection efficiency of BMP expressing DNA using a vector-loaded collagen sponge model. BMP-2/pcDNA3.1 plasmid was constructed by subcloning human BMP-2 cDNA into the pcDNA3.1 plasmid vector. PEI/DNA complexes were prepared by mixing PEI and BMP-2/pcDNA3.1 and the constructed complexes were loaded into the collagen sponges. In vitro studies, BMSCs were transfected with the PEI/BMP-2/pcDNA3.1 complexes from collgen sponge. The level of secreted BMP-2 and alkaline phosphatase activities of transfected BMSCs were significantly higher in PEI/BMP-2/pcDNA3.1 group than in BMP-2/pcDNA3.1 group (p<0.05). Transfected BMSCs were cultured and mineralization was observed only in cells treated with PEI/BMP-2/pcDNA3.1 complexes. In vivo studies, PEI/BMP-2/pcDNA3.1/collagen, BMP-2/pcDNA3.1/collagen and blank collagen were grafted in skeletal muscle of nude mice. Ectopic bone formation was shown in PEI/BMP-2/pcDNA3.1/collagen grafted mouse 4 weeks postimplantation, while not in BMP-2/pcDNA3.1 grafted tissue. This study suggests that PEI-condensed DNA encoding for BMP-2 is capable of inducing bone formation in ectopic site and might increase the transfection rate of BMP-2/pcDNA3.1. As a non-viral vector, PEI offers the potential in gene therapy for bone engineering.

• The Journal of Korean Obstetrics and Gynecology
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• v.31 no.1
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• pp.20-42
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• 2018

Objectives: This study was designed to investigate anti-obesity effects the improvement effects of Gyejibongnyeong-hwan and Gyejibongnyeong-hwan-Gangji-hwan (CIPPDF) in a ob/ob mouse model. Methods: Seven-week old mice (wild-type C57BL/6J and ob/ob) were used for all experiments. Wild-type C57BL/6J mice were used as normal group and obese ob/ob mice were randomly divided into 4 groups. a normal group given a standard diet, an obese control group given a standard diet with CIPP (300 mg/kg), CIPPDF (1) (300+300 mg/kg), CIPPDF (2) (300+600 mg/kg) respectively. After 10 weeks of treatment, body weight gain, feeding efficiency ratio, blood lipid markers, mRNA levels of genes involved in fatty acid ${\beta}-oxidation$ and lipogenesis in in-vivo, were examined. Results: 1. Body weight gain and Feeding efficiency ratio were significantly decreased in CIPPDF (1) compared with control. Fat mass was significantly decreased in CIPPDF (2) in EAT compared with control. 2. Consistent their effects on body weight gain and fat mass, circulating concentrations of LDL-cholesterol were decreased in CIPPDF (1), CIPPDF (2) groups compared with control. 3. MCAD mRNA levels of genes was increased in CIPPDF (1), CIPPDF (2) groups in the liver, epididymal adipose tissue compared with control. VLCAD mRNA levels of genes was increased in CIPPDF (1), CIPPDF (2) groups in the skeletal muscle compared with control. 4. $PPAR{\gamma}$ mRNA was decreased in CIPPDF (1) in the liver compared with control. SCD1 mRNA was decreased in CIPPDF (1), CIPPDF (2) groups in the epididymal adipose tissue compared with control. Conclusions: In conclusion, These results suggest that CIPPDF not only decrease feeding efficiency ratio, and LDL-cholesterol, but also reduce EAT fat mass contributing to the improvement of ovesity. CIPPDF also were increased in mRNA levels of genes involved in fatty acid ${\beta}-oxidation$ and decreased in mRNA levels of genes involved in lipogenesis.

• Journal of Biomedical Engineering Research
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• v.25 no.6
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• pp.479-487
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• 2004

Biopotential signals have been used as command in systems using electrical stimulation of motor nerves to restore movement after an injury to the central nervous system (CNS). In order to use the voluntary EMG (electromyography) among the biopotentials as a control signal for the electrical stimulation of the same muscle for CNS injury patients, it is necessary to remove M-wave of having high magnitude from raw data. We designed an optimal filter for removing the M-wave and preserving the voluntary EMG and showed that the optimal filter is eigen filter. We also proved that the previous method using the prediction error filter(PEF) is a suboptimal filtering in the sense of preserving the voluntary EMG. On basis of the data obtained from a model for M-wave and voluntary EMG and from actual CNS injury patients, with false-positive rate analysis, the proposed adaptive filter showed a very promising performance in comparison with previous method.

• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.1
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• pp.174-180
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• 2006

We examine the effect of Geiji-Bokryung-Hwan(GBH) on erectile function in a rat model of hypercholesterolemic erectile dysfunction. GBH, a drug preparation consisting of five herbs of Cinnamomi Ramulus (Geiji), Poria Cocos (Bokryun), Mountan Cortex Radicis (Mokdanpi), Paeoniae Radix (Jakyak), and Persicae Semen (Doin) is a traditional Korean herbal medicine that is widely used in the treatment of atherosclerosis-related disorders. In this study, 3-month-old Sprague-Dawley rats were used. The 6 rats control animals were fed a normal diet and the other 18 rats were fed 1% cholesterol diet for 3 months. After 1 months, GBH was added to the drinking water of the treatment group of 12 rats but not the cholesterol only group of 6 rats. Of the 12 rats 6 received 30 mg/kg per day (group 1) and 6 received 60 mg/kg per day (group 2) of GBH. At 3 months erectile function was evaluated with cavernous nerve electrostimulation in all animals. Penile tissues were collected for electron microscopy, and to perform Western blot for endothelial nitric oxide synthase (eNOS), neuronal nitric oxide synthase (nNOS), basic fibroblast growth factor (bFGF) and caveolin-1. Systemic arterial pressure was not significantly different between the animals that were fed the 1% cholesterol diet and the controls. Conversely erectile function was not impaired in the herbal medicine treated rats. Electron microscopy showed many caveolae with fingerlike processes in the cavernous smooth muscle and endothelial cell membranes in control and treated rats but not in the cholesterol only group of rats. Western blot showed differences among groups in protein expression for eNOS, nNOS, caveolin-1 and bFGF protein expression in penile tissue. Increased eNOS and nNOS protein expressions dy high cholesterol diet were significantly decreased in group 1 and group 2. Interestingly, caveolin-1 and bFGF protein expression was significantly higher in groups 1 and 2 than in the cholesterol only and control groups.

• Korean Journal of Food Science and Technology
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• v.38 no.1
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• pp.147-151
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• 2006

Effects of wild plant extract (Lak) based on Korea traditional prescription on maximal exercise performance and endurance were evaluated using calorie-restriction animal model. In acute forced swimming test with 10% body weight attached to tail, dietary Lak supplementation increased exercise performance endurance by increasing concentrations of ATP and insulin-like growth factor-1 (IGF-1) under calorie-restriction condition, and decrement of blood lactic acid concentration and increment of muscle ATP content were observed. These results suggest Lak is very effective for decreasing side-effects of obesity therapy using very low calorie diet.

• Journal of Biomedical Engineering Research
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• v.22 no.1
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• pp.49-58
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• 2001

A new scale named LCL(Logically Classified Level) has been developed to judge the status of the spasticity quantitatively implementing a pcndulum test in this study. Total 30 parameters have been used to develop the new scale. One representative parameter that can represent the dominant characteristics of spasticity has been chosen through statistical analysis. 20 parameters among 30 parameters uscd in the statistical analysis were newly developed and 10 parameters were from previous studies. The new 20 parametcrs were developed using combinations of pcndulum test characteristics. ie anglc, angular velocity. musclo midel. and EMG. 11 parameters among 30 parameters have showed strong correlations each other, Finally. L11 that showed consistency at every case has been chosen to be a representative parameter among the 11 parameters. 28 patients data were separated into 4 groups. A regression equation to predict the trend of patients of L11 has been made. The paramcter L11 was tested to prove its usabilitics for various cases of patients. The new LCL scale is expected to be a quantitative scale, and to replace the MAS(Modified Ashworth Scale) that is not a quantitative scale. Especially it is also expected that the new scale could be used to plan a treatmcnt period. methods, and intensity. as it can evaluate the status of patient's in detail.

• Journal of agriculture & life science
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• v.46 no.5
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• pp.57-63
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• 2012

The purpose of this study was to estimate genetic parameters for productive traits in Duroc breed. In this study, 40,657 records for productive traits and the pedigree data of 47,974 families were collected from 41 farms registered at the Korean Animal Improvement Association (KAIA) from 2004 to 2011. The REMLf90 program was used to analyze a multiple traits animal model with fixed effects of sex, contemporary group, parity and age at the end of the test as covariate and random effects of animal and residual error. The heritabilities of days to 90 kg (D90KG), average daily gain (ADG), backfat thickness (BF) and eye muscle areas (EMA) were estimated to be 0.334, 0.340, 0.335, and 0.200, respectively. The genetic correlation coefficients were -0.992 between D90KG and ADG, -0.142 between ADG and BF, -0.361 between ADG and EMA, and -0.243 between BF and EMA. Conversely, positive genetic correlations for D90KG with BF and EMA were 0.13 and 0.36, respectively.

• The Korea Journal of Herbology
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• v.28 no.4
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• pp.83-92
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• 2013

Objectives : Lespedeza Cuneata has been used to treat leukorrhea, asthma, stomach pain, diarrhea, acute mastitis, in Korean traditional medicine. According to recent studies, Lespedeza Cuneata has antioxidation, hypoglycemia, cell protective, insulin secretion, whitening, corpora cavernosa smooth muscle relaxation and antimicrobial activities, but it has been rarely conducted to evaluate the immuno-biological activity. The present study was examined to evaluate the anti-inflammatory effects of the Lespedeza Cuneata MeOH extract (LCE) in vivo and in vitro. Methods : In vitro, inflammatory mediators, such as cytokines, nitric oxide and prostaglandin $E_2$ were detected after the addition of LPS with or without LCE in Raw 264.7 macrophage cell line. In vivo, anti-edema effect of LCE was determined in the carrageenan-induced paw edema model in rats. Results : In vitro assay, LCE decreased release of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) via suppression of iNOS and COX-2 expression. LCE inhibited the phosphorylation of $I{\kappa}B$ indicating the suppression of NF-${\kappa}B$ pathway. In vivo assay, LCE significantly inhibited the formation of paw edema induced by carrageenan injection in rats. LCE effectively inhibited increases of hind paw skin thickness and inflammatory cell infilterations. Conclusion : These findings demonstrate that LCE has inhibitory effect on inflammatory mediators in LPS-activated Raw 264.7 cells and on paw edema in carrageenan-stimulated rats, showing the possibility of anti-inflammatory use of Lespedeza Cuneata.

• Journal of Korea Entertainment Industry Association
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• v.14 no.8
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• pp.447-454
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• 2020

The purpose of this study was to determine the clinical effects of blood flow regulation exercise for improving patients' health care and its usefulness as a rehabilitation model for various diseases by analyzing and examining the existing literature. A literature review of Korean academic journals published over a 10-year period, from 2010 to 2019, was conducted using words such as "blood flow regulation," "blood flow restriction," "low-intensity exercise," and "Kaatsu." Kaatsu is a blood flow regulation exercise developed in 1966 by Dr. Yoshiaki Sato of Japan. It is an efficient and effective exercise method that uses blood flow regulation bands that increase the secretion of growth hormones to develop muscles within a short time, improves blood circulation and metabolism to prevent and improve adult diseases, shortens the rehabilitation period, and improves cardiovascular function. The study participants consisted of 10 patients, of whom four were elderly, four had obesity, one was a stroke patient, and one was a trauma patient. The results of this study show that the blood flow regulation exercise, which is a low-intensity exercise, has the same effect as high-intensity exercise, which supports the evidence that it is a highly efficient exercise method for muscle development and rehabilitation of the elderly, adolescents, and patients with injuries who have difficulty in general exercising. For future studies, further reviews are necessary to verify the effectiveness of the exercise method according to blood flow regulation site and type of disease.