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http://dx.doi.org/10.6116/kjh.2013.28.4.83.

Anti-inflammatory effects of Lespedeza Cuneata in vivo and in vitro  

Lee, Hoil (Daegu Haany University, College of Oriental Medicine, Dept. of ophthalmology, otolaryngology and dermatology)
Jung, Ji Yun (Daegu Haany University, College of Oriental Medicine, Dept. of Herbal formula)
Hwangbo, Min (Daegu Haany University, College of Oriental Medicine, Dept. of ophthalmology, otolaryngology and dermatology)
Ku, Sea Kwang (Daegu Haany University, College of Oriental Medicine, Dept. of Anatomy)
Kim, Young Woo (Daegu Haany University, College of Oriental Medicine, Dept. of Herbal formula)
Jee, Seon Young (Daegu Haany University, College of Oriental Medicine, Dept. of ophthalmology, otolaryngology and dermatology)
Publication Information
The Korea Journal of Herbology / v.28, no.4, 2013 , pp. 83-92 More about this Journal
Abstract
Objectives : Lespedeza Cuneata has been used to treat leukorrhea, asthma, stomach pain, diarrhea, acute mastitis, in Korean traditional medicine. According to recent studies, Lespedeza Cuneata has antioxidation, hypoglycemia, cell protective, insulin secretion, whitening, corpora cavernosa smooth muscle relaxation and antimicrobial activities, but it has been rarely conducted to evaluate the immuno-biological activity. The present study was examined to evaluate the anti-inflammatory effects of the Lespedeza Cuneata MeOH extract (LCE) in vivo and in vitro. Methods : In vitro, inflammatory mediators, such as cytokines, nitric oxide and prostaglandin $E_2$ were detected after the addition of LPS with or without LCE in Raw 264.7 macrophage cell line. In vivo, anti-edema effect of LCE was determined in the carrageenan-induced paw edema model in rats. Results : In vitro assay, LCE decreased release of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) via suppression of iNOS and COX-2 expression. LCE inhibited the phosphorylation of $I{\kappa}B$ indicating the suppression of NF-${\kappa}B$ pathway. In vivo assay, LCE significantly inhibited the formation of paw edema induced by carrageenan injection in rats. LCE effectively inhibited increases of hind paw skin thickness and inflammatory cell infilterations. Conclusion : These findings demonstrate that LCE has inhibitory effect on inflammatory mediators in LPS-activated Raw 264.7 cells and on paw edema in carrageenan-stimulated rats, showing the possibility of anti-inflammatory use of Lespedeza Cuneata.
Keywords
Lespedeza Cuneata; anti-inflammation; NF-${\kappa}B$; LPS; Carrageenan;
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Times Cited By KSCI : 5  (Citation Analysis)
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