• Journal of Korean Academy of Nursing
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• v.41 no.6
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• pp.834-842
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• 2011

Purpose: The purpose of this study was to determine the effect of dehydroepiandrosterone (DHEA) on recovery of muscle atrophy induced by Parkinson's disease. Methods: The rat model was established by direct injection of 6-hydroxydopamine (6-OHDA, 20 ${\mu}g$) into the left striatum using stereotaxic surgery. Rats were divided into two groups; the Parkinson's disease group with vehicle treatment (Vehicle; n=12) or DHEA treatment group (DHEA; n=22). DHEA or vehicle was administrated intraperitoneally daily at a dose of 0.34 mmol/kg for 21 days. At 22-days after DHEA treatment, soleus, plantaris, and striatum were dissected. Results: The DHEA group showed significant increase (p<.01) in the number of tyrosine hydroxylase (TH) positive neurons in the lesioned side substantia nigra compared to the vehicle group. Weights and Type I fiber cross-sectional areas of the contralateral soleus of the DHEA group were significantly greater than those of the vehicle group (p=.02, p=.00). Moreover, extracellular signal-regulated kinase (ERK) phosphorylation significantly decreased in the lesioned striatum, but was recovered with DHEA and also in the contralateral soleus muscle, Akt and ERK phosphorylation recovered significantly and the expression level of myosin heavy chain also recovered by DHEA treatment. Conclusion: Our results suggest that DHEA treatment recovers Parkinson's disease induced contralateral soleus muscle atrophy through Akt and ERK phosphorylation.

• Molecules and Cells
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• v.41 no.3
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• pp.198-206
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• 2018

Aortic dissection (AD) is a catastrophic disease with high mortality and morbidity, characterized with fragmentation of elastin and loss of smooth muscle cells. Although AD has been largely attributable to polymorphisms defect in the elastin-coding gene, tropoelastin (TE), other undermined factors also appear to play roles in AD onset. Here, we investigated the effects of post-transcriptional control of TE by microRNAs (miRNAs) on elastin levels in aortic smooth muscle cells (ASMC). We found that miR-144-3p is a miRNA that targets TE mRNA in both human and mouse. Bioinformatics analyses and dual luciferase reporter assay showed that miR-144-3p inhibited protein translation of TE, through binding to the 3'-UTR of the TE mRNA. Interestingly, higher miR-144-3p levels and lower TE were detected in the ASMC obtained from AD patients, compared to those from non-AD controls. In a mouse model for human AD, infusion of adeno-associated viruses (serotype 6) carrying antisense for miR-144-3p (asmiR-144-3p) under CAG promoter significantly reduced the incidence and severity of AD, seemingly through enhancement of TE levels in ASMC. Thus, our data suggest an essential role of miR-144-3p on the pathogenesis of AD.

• The Journal of Korean Academy of Prosthodontics
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• v.25 no.1
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• pp.213-226
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• 1987

The purpose of this study was to standardize and classify the coordination pattern among the left and right masseter and anterior temporal muscles, in terms of integrated EMG values per stroke during gum chewing in normal subjects. In this study, 20 normal subjects were selected to chew a piece of gum and integrated EMG from middle portion of the masseter and anterior temporal muscles on both sides were recorded 20 times during each of the right and left chewing respectively. And the Bioelectric Processor Model EM2 (Myo-tronies Research, Inc., U.S.A.) with the surface electrodes was used to record the EMG activity during all experimental procedures. The results were as follows; 1. In all subjects, the chewing side masseter muscle was predominantly active among the four muscles examined. 2. The integrated EMG value of the middle of masseter muscle was significantly predominant than those of the anterior temporal muscle (p<0.0001) on the chewing side, but no significant predominant was observed on the nonchewing side (p<0.98). 3. In comparison with same muscles on the chewing and nonchewing side, low correlation coefficient was found between the middle of masseter muscles (R=0.317), but high correlation coefficient was found between the anterior temporal muscles (R=0.738). Between two muscles on the same side, there were significant correlation in the chewing (R=0.557) and nonchewing side (R=0.625). 4. In the coordination patterns among four muscles examined, distinct individual differences were found, but in an identical subject one fixed pattern appeared with significant reproducibility.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.6
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• pp.962-969
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• 2010

The purpose of this investigation was to confirm the effect of Nelumbinis Semen on the recovery of the cardiac muscle activity. We studied the effect of Nelumbinis Semen on the recovery of ischemic SD rat hearts perfused with Nelumbinis Semen, using a model of ex-vivo perfusion (Non-working Langendorff perfusion system) and working heart perfusion system at the same time. To explore the effect of Nelumbinis Semen at the level of proteome, two-dimensional electrophoresis and MALDI-TOF analysis were performed. We found out that the proteins increased after perfusion of Nelumbinis Semen are Mitochondrial aconitase, ATP synthase alpha chain, Lactate dehydrogenase B, Creatine kinase, Glyceraldehyde 3-phosphate dehydrogenase, Alpha B-crystallin, Myosin and Heart fatty acid binding protein. Almost, all of them are concerned with ATP production in the cardiac muscle with glucose metabolism.

• Journal of muscle and joint health
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• v.2 no.2
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• pp.131-146
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• 1995

Many arthritis patients experience weakness of muscles in extremities mainly due to disuse atrophy and weight gain because of the limited activities and exercises. This study examines the effects of the 6-week aquatic-exercise program on the body fat and the muscle of the rheumatoid arthritis patients. Seventeen patients in the experimental group and 18 in the control group were assigned depending on their preference and physical condition. These patients had more than 3 points of pain out of 10, deformities in knee, wrist and ankle joints. The amount of aquatic exercise increases from 35 minutes in the first week to 60 minutes in the 6th week. In the resting period they discussed their own experiences about exercise, personal and family affairs, and performed some recreation programs in order to increase the self-efficacy and promote the relationship with other patients by the group activities. Skinfold thickness and circumferences of both extremities were measured before and after experiment to compare the difference. For testing the body fat Saham Model was used. Prior to the experiment two group's body weight, skinfold thickness and skin circumferences were not significantly different which indicates the homogeneity of two groups. Body weight and most parts of skinfold thickness of the experimental group were significantly lower than the control group after 6-week aquatic exercise program. Circumference was not significantly lower than the control after the program. These findings indicate the in-crease of muscle sizes and the reduction of the body fat. Therefore a more active application of aquatic exercise into a variety of clients is strongly suggested.

• Genomics & Informatics
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• v.11 no.4
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• pp.263-271
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• 2013

We investigated the contribution of genetic variations of KLF5 to basal metabolic rate (BMR) and resting metabolic rate (RMR) and the inhibition of obesity in Korean children. A variation of KLF5 (rs3782933) was genotyped in 62 Korean children. Using multiple linear regression analysis, we developed a model to predict BMR in children. We divided them into several groups; normal versus overweight by body mass index (BMI) and low BMR versus high BMR by BMR. There were no differences in the distributions of alleles and genotypes between each group. The genetic variation of KLF5 gene showed a significant correlation with several clinical factors, such as BMR, muscle, low-density lipoprotein cholesterol, and insulin. Children with the TT had significantly higher BMR than those with CC (p=0.030). The highest muscle was observed in the children with TT compared with CC (p=0.032). The insulin and C-peptide values were higher in children with TT than those with CC (p=0.029 vs. p=0.004, respectively). In linear regression analysis, BMI and muscle mass were correlated with BMR, whereas insulin and C-peptide were not associated with BMR. In the high-BMR group, we observed that higher muscle, fat mass, and C-peptide affect the increase of BMR in children with TT (p < 0.001, p < 0.001, and p=0.018, respectively), while Rohrer's index could explain the usual decrease in BMR (adjust $r^2$=1.000, p < 0.001, respectively). We identified a novel association between TT of KLF5 rs3782933 and BMR in Korean children. We could make better use of the variation within KLF5 in a future clinical intervention study of obesity.

• Journal of the Korean Society of Physical Medicine
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• v.5 no.2
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• pp.223-231
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• 2010

Purpose : The Purpose of this study was to compare muscle activation and foot pressure on baby carrier and sling for baby care. Methods : Thirty one women subjects (mean age 23.2 years) participated in four static conditions : unloaded quite standing, carrying an anterior baby carrier, carrying a posterior baby carrier, and sling. The baby carrier and sling were loaded with baby model that filled 7.6kg loads. Surface electromyography was used to measure activity in the internal oblique, T4, L3, L5 paraspinal muscle, vastus medialis, biceps femoris, tibialis anterior, and gastrocnemius for four conditions. And foot pressure was measured by using MatScan system(Tekscan, USA). Results : The activation of Biceps femoris, T4, L3, and L5 paraspinal muscle were significant differences(p<.05), but other muscles were no significant differences in four conditions(p>.05). Right foot contact area and peak pressure of right mid foot area were significant differences in four conditions(p<.05). Conclusion : The results of this study indicate that the use of baby carrier of sling for baby care were influenced postural responses of young women. Further work is recommended to find out the influences of various assistive devices for baby care.

• The Journal of Korean Physical Therapy
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• v.33 no.6
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• pp.292-296
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• 2021

Purpose: The purpose of this study was to investigate the exercise effect of two types of training with a recumbent cycle ergometer on ankle muscle strength (dorsiflexor strength, DFS; dorsiflexor strength/weight, DFS/kg; plantar flexor strength, PFS; and plantar flexor strength/weight, PFS/kg) in healthy male subjects. Methods: Twenty-three healthy males (27.91±8.66 yr) were randomly allocated into two groups (high-intensity interval training (HIIT), and aerobic exercise training (AET) after the first measurement. The subjects were trained for 24 sessions (40 min/rep, three times/week) and ankle strength was measured for a second time. Two-way mixed model analysis of variance (ANOVA) was used to identify significant differences between changes in ankle muscle strength between before and after training (within factors) in the HIIT and AET groups (between factors). The statistical significance level was set at α=0.05. Results: In both HIIT and AET groups, all variables of ankle muscle strength were significantly increased after training compared to before training (p=0.001). However, there were no differences in all variables of ankle strength between the HIIT and AET group (p>0.05). Conclusion: Both types (HIIT and AET) of recumbent cycle exercise training could be effective training methods to increase ankle muscle strength in healthy individuals, and the HIIT type with high intensity and low frequency pedaling could be recommended more to strengthen ankle muscles.

• Journal of muscle and joint health
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• v.29 no.3
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• pp.165-175
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• 2022

Purpose: This study was conducted to identify differences in health behavior, health-related quality of life, and depression between adult men and women during the COVID-19 pandemic. Methods: Data were obtained from 1,927 men and 2,314 women aged 19 to 64 who participated in the 7th Korea National Health and Nutrition Survey in 2020. Data were analyzed using complex sample x² test, complex sample general linear model, and complex sample logistic regression. Results: During the pandemic, men drank more alcohol (OR=2.34, 95% CI: 1.89~2.91) and smoked more (OR=7.89, 95% CI: 6.16~10.09) than women. In addition, men were 2.45 times more exposed to second-hand smoke at the workplace (95% CI: 1.78~3.37) and 1.4 times more exposed in public places (95% CI: 1.12~1.77). The rate of muscle exercise was higher among men than women (OR=2.09, 95% CI: 1.76~2.49). No difference in health-related quality of life was observed between men and women (t=0.17, p=.78), but women had higher depression scores (t=-3.14, p=.002). Conclusion: Customized sex-specific interventions should be developed during the COVID-19 pandemic. This kind of infectious disease disaster requires plans designed to reduce alcohol intake and smoking among men and interventions aimed at increasing muscle exercise among women. Strategies should also be formulated to reduce depression among women.

• The Korean Journal of Physiology and Pharmacology
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• v.5 no.2
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• pp.183-188
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• 2001

To elucidate antidiabetic effect and mechanism(s) of acarbose in a polygenic spontaneous hyperglycemic and hyperinsulinemic diabetic animal model, $KKA^y$ mice, acarbose was administered orally for 4 weeks and effects on body weight, plasma glucose and insulin levels, genetic expressions of intestinal sucrase-isomaltase (SI), sodium-glucose cotransporter (sGLT1) and glucose transporter in quadriceps muscle (GLUT4) were examined in this study. Although no differences in body weight were detected between control and acarbose-treated groups, plasma glucose level in acarbose-treated group was markedly reduced as compared to the control. In the mechanism study, acarbose downregulated the SI and SGLT1 gene expressions, and upregulated the GLUT4 mRNA and protein expressions when compared to the control group. In conclusion, the data obtained strongly implicate that acarbose can prevent the hyperglycemia in $KKA^y$ mice possibly through blocking intestinal glucose absorption by downregulations of SI and sGLT1 mRNA expressions, and upregulation of skeletal muscle GLUT4 mRNA and protein expressions.