Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect Of Nelumbinis Semen On The Recovery Of The Cardiac Muscle Activity by Proteome Analysis

연자육(蓮子肉)의 심근 경색 모델에 대한 Proteom 분석

  • Ahn, Chang-Joon (Department of Physiology, College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Gi-Hyun (Department of Physiology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Yang-Seok (Department of Physiology, College of Oriental Medicine, Kyung Hee University) ;
  • Hong, Moo-Chang (Department of Physiology, College of Oriental Medicine, Kyung Hee University) ;
  • Bae, Hyun-Su (Department of Physiology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Jong-Hoon (Department of Veterinary Physiology, College of Veterinary Medicine, Chonbuk National University) ;
  • Shin, Min-Kyu (Department of Physiology, College of Oriental Medicine, Kyung Hee University)
  • 안창준 (경희대학교 한의과대학 생리학교실) ;
  • 이기현 (경희대학교 한의과대학 생리학교실) ;
  • 김양석 (경희대학교 한의과대학 생리학교실) ;
  • 홍무창 (경희대학교 한의과대학 생리학교실) ;
  • 배현수 (경희대학교 한의과대학 생리학교실) ;
  • 김종훈 (전북대학교 수의과대학 수의생리학교실) ;
  • 신민규 (경희대학교 한의과대학 생리학교실)
  • Received : 2010.08.23
  • Accepted : 2010.11.04
  • Published : 2010.12.25
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Abstract

The purpose of this investigation was to confirm the effect of Nelumbinis Semen on the recovery of the cardiac muscle activity. We studied the effect of Nelumbinis Semen on the recovery of ischemic SD rat hearts perfused with Nelumbinis Semen, using a model of ex-vivo perfusion (Non-working Langendorff perfusion system) and working heart perfusion system at the same time. To explore the effect of Nelumbinis Semen at the level of proteome, two-dimensional electrophoresis and MALDI-TOF analysis were performed. We found out that the proteins increased after perfusion of Nelumbinis Semen are Mitochondrial aconitase, ATP synthase alpha chain, Lactate dehydrogenase B, Creatine kinase, Glyceraldehyde 3-phosphate dehydrogenase, Alpha B-crystallin, Myosin and Heart fatty acid binding protein. Almost, all of them are concerned with ATP production in the cardiac muscle with glucose metabolism.

Keywords

References

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