• Journal of Korean Academy of Nursing
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• v.37 no.1
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• pp.81-90
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• 2007

Purpose: The purpose of this study was to examine the effects of daily exercise before steroid treatment on mass, the type I and II fiber cross-sectional area, and myofibrillar protein content of hindlimb muscles in a rat model. Method: Adult male Sprague-Dawley rats were randomly assigned to one of three groups: a control group(n=10) that had a normal saline injection for 7days, a steroid group(n=10) that had a steroid injection for 7days, and an exercise-steroid group(n=10) that ran on the treadmill for 7days before a steroid treatment. Body weight and food intake were measured every day. At 15 days all rats were anesthetized and the soleus, plantaris and gastrocnemius muscles were dissected. Result: The exercise-steroid group showed significant increases as compared with the steroid group in body weight, muscle weight of the soleus and gastrocnemius, type II muscle fiber cross-sectional area of plantaris, and myofibrillar protein content of the soleus, plantaris, and gastrocnemius. As compared with the control group, the steroid group showed significant decreases in body weight and diet intake, muscle weight, the type II fiber cross-sectional area and myofibrillar protein content of the soleus, plantaris, and gastrocnemius muscles. Conclusion: Daily exercise before steroid treatment attenuates hindlimb muscle atrophy, with type II muscle changes more apparent than type I muscle changes.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.37
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• pp.46.1-46.6
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• 2015

Background: The purpose of this study was to evaluate the change of food intake after different dosages of botulinum toxin A (BTX) injection in the animal model. Additionally, the dimensional and histological change at 14 days after BTX injection was also evaluated. Methods: The comparative study was performed using the BTX injection model in rats (n = 5 for each group). Group 1 was the saline-injected group. Group 2 was the 5-unit BTX-injection group to each masseter muscle. Group 3 was the 10-unit BTX-injection group to each masseter muscle. Food intake rates and body weight were checked daily before and after BTX injection until 10 days. All animals were sacrificed at 14 days after BTX injection, and the specimens underwent hematoxylin and eosin stain and immunohistochemical staining for myosin type II (MYH2). Results: The recovery of food intake in groups 2 and 3 decreased significantly compared with group 1 from day 2 to day 7 and day 9 after injection (p < 0.05). The BTX-treated masseter muscles were significantly smaller than those in group 1 (p = 0.015). The immunohistochemical findings demonstrated that the expression of MYH2 was significantly higher in group 3 compared to groups 1 and 2 (p < 0.001). Conclusions: BTX injection to the masseter muscle in rats demonstrated short food-intake-rate reduction with recovery until 10 days after injection. The thickness of the masseter muscle and MYH2 expression were significantly changed according to the injected dose of BTX.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.15 no.1
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• pp.61-70
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• 2005

This study investigated the spinal loads(L5/S1 disc compression and shear forces) predicted from four biomechanical models: one EMG model and three optimization models. Three objective functions used in the optimization models were to miminize 1) the cubed muscle forces : MF3, 2) the cubed muscle stress : MS3, 3) maximum muscle intensity : MI. Twelve healthy male subjects participated in the isometric voluntary exertion tests to six directions : flexion/extension, left/right lateral bending, clockwise/ counterclockwise twist. EMG signals were measured from ten trunk muscles and spinal loads were assessed at 10, 20, 30, 40, 50, 60, 70, 80, 90%MVE(maximum voluntary exertion) in each direction. Three optimization models predicted lower L5/S1 disc compression forces than the EMG model, on average, by 31%(MF3), 27%(MS3), 8%(MI). Especially, in twist and extension, the differences were relatively large. Anterior-posterior shear forces predicted from optimization models were lower, on average, by 27%(MF3), 21%(MS3), 9%(MI) than by the EMG model, especially in flexion(MF3 : 45%, MS3 : 40%, MI : 35%). Lateral shear forces were predicted far less than anterior-posterior shear forces(total average = 124 N), and the optimization models predicted larger values than the EMG model on average. These results indicated that the optimization models could underestimate compression forces during twisting and extension, and anterior-posterior shear forces during flexion. Thus, future research should address the antagonistic coactivation, one major reason of the difference between optimization models and the EMG model, in the optimization models.

• Clinics in Shoulder and Elbow
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• v.21 no.2
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• pp.87-94
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• 2018

Background: Popeye deformity is common after rupture of the biceps muscle's long head tendon. Herein, we report on histological changes in biceps brachii muscles following tenotomy of the long head biceps tendon. Methods: Twelve Sprague-Dawley rats (12-week-old) underwent tenotomy of the long head biceps tendon in the right shoulder. At postoperative weeks 4, 7, and 10, the operative shoulders were removed by detaching the biceps brachii muscle from the glenoid scapula and humerus; the opposite shoulders were removed as controls. H&E staining was performed to elucidate histological changes in myocytes. Oil-red O staining was performed to determine fatty infiltration. Myostatin antibody immunohistochemistry staining was performed as myostatin is expressed by skeletal muscle cells during myogenesis. Results: H&E staining results revealed no changes in muscle cell nuclei. There were no adipocytes detected. Compared with that of the control biceps, the cross-sectional area of the long head biceps was significantly smaller (p=0.00). Statistical changes in the total extent of the 100 muscle cells were significant (p=0.00). Oil-red O staining revealed no fatty infiltration. Myostatin antibody immunohistochemical staining revealed no significant difference between the two sides. Conclusions: Muscular changes after tenotomy of the long head biceps included a decrease in the size of the individual muscle cells and in relative muscle mass. There were no changes observed in muscle cell nuclei and no fatty infiltration. Moreover, there were no changes detected by myostatin antibody immunohistochemistry assay.

• Animal Bioscience
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• v.34 no.8
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• pp.1309-1320
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• 2021

Objective: Investigation of muscle growth at different developmental stages is an appropriate strategy for studying the mechanisms underlying muscle development and differences in phenotypes. In particular, the muscle development mechanisms and the difference between the fastest and slowest growth rates. Methods: In this study, we used a growth curve model to fit the growth inflection point (IP) of QingYu pigs and compared differences in the long non-coding RNA (lncRNA) transcriptome of muscle both at the growth IP and plateau phase (PP). Results: The growth curve of the QingYu pig had a good fit (R² = 0.974) relative to a typical S-curve and reached the IP at day 177.96. At the PP, marbling, intramuscular fat, and monounsaturated fatty acids had increased significantly and the percentage of lean muscle and polyunsaturated fatty acids had decreased. A total of 1,199 mRNAs and 62 lncRNAs were differentially expressed at the IP compared with the PP. Additional to gene ontology and Kyoto encyclopedia of genes and genomes pathway analyses, these differentially expressed protein coding genes were principally related to muscle growth and lipid metabolism. Conclusion: Our results suggest that the identified differentially expressed lncRNAs, could play roles in muscle growth, fat deposition and regulation of fatty acid composition at the IP and PP.

• Journal of Mechanical Science and Technology
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• v.14 no.3
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• pp.338-349
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• 2000

Neck muscle forces and spinal loads at the C4/5 level were estimated that result from isometric voluntary ramp efforts gradually developing to maximums in flexion, extension, left lateral bending and right lateral bending. Electromyographic (EMG) activities, a three-dimensional anatomic data of the neck and a hybrid model, EMG-assisted optimization (EMGAO) model, were used. The model computed the cervical loads at 25%,50%,75%, and 100% of peak moments. The highest model-predicted C4/5 joint compressive forces occurred during flexion; $361\;({\pm}164)\;N,\;811\;({\pm}288)\;N,\;1207\;({\pm}491)\;N\;and\;1674\;({\pm}319)\;N$ in 25%, 50%, 75% and 100% of peak moment respectively. Variations in load distribution among the agonistic muscles and co-contractions of antagonistic muscles were estimated during ramp efforts. Results suggest that higher C4/5 joint loads than previously reported are possible during isometric, voluntary muscle contractions. These higher physiological loads at C4/5 level must be considered possible during orthopedic reconstruction at this level.

• Fashion & Textile Research Journal
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• v.9 no.4
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• pp.450-454
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• 2007

The purpose of this study was to offer objective information about standard of selecting and training fashion models by verifying anthropometric characteristics and comparing with general women. Total forty eight fashion models (M=20.08yr ; SD=1.7) were recruited by convenience sampling. Thirty eight items of participants were measured by manual of International Society for the Advancement of Kinanthropometric (ISAK). Mean and standard deviation of 38 items and also factor of somatotype (endomorph, mesomorph, ectomorph) were calculated. In the result, somatotype of fashion model was endomorphic ectomorphy, less muscle and taller height. Conclusionally, this study indicated that there is less mesomorphy rating related to muscle quantity, bone mass in somatoplot. However, it is necessary to provide the program enhancing the physical activity with model department.

• Journal of the Ergonomics Society of Korea
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• v.13 no.2
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• pp.65-79
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• 1994

A biomechanical model of lower extremity in seated postures was developed to assess muscular activities of lower extremity involved in a variety of foot pedal operations. The model incorporated four rigid body segments with the twenty-four muscles to represent lower extremity. This study deals with quasi-static movement to investigate dymanic movement effect in seated foot operation. It is found that optimization method which has been used for modeling the articulated body segments does not predict the forces generated from biarticular muscles and antagonistic muscles reasonably. So, the revised nonlinear optimization scheme was employed to consider the synergistic effects of biarticular muscles and the antagonistic muscle effects from the stabilization of the joint. For the model validation, three male subjects performen the experiments in which EMG activities of the nine lower extremity muscles were measured. Predicted muscle forces were compared with the corresponding EMG amplitudes and it showed no statistical difference. For the selection of optimal seated posture, a physiological meaningful criterion for muscular load sharing developed.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.39 no.3
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• pp.215-221
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• 2015

The elbow reflex, a principal reflex in the upper extremity, plays an important role in the diagnosis of cervical spine syndromes. In this study, the muscle activations of brachial biceps and triceps, and the kinematics of upper extremities were predicted using an activation model for the stretch reflex. The muscle activations that equated the simulation results estimated by the analysis model with the experimental results were obtained first, and the activations obtained from the simulations were compared with the electromyography signals obtained from the experiments, for model validation. The root mean squares error of the joint angles (obtained from experiments and simulation using the suggested model) was 0.056, a value that is half of that obtained using the previous model. This demonstrates that the suggested model corresponded well with the actual reflex.

• Journal of Korean Institute of Industrial Engineers
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• v.25 no.3
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• pp.330-335
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• 1999

The goal of the study is to determine the most sensitive parameter to represent the degree of muscle force and fatigue. Various numerical parameters such as the first coefficient of Autoregressive (AR) Model, Root Mean Square (RMS), Zero Crossing Rate (ZCR), Mean Power Frequency (MPF), Median Frequency (MF) were tested in this study. Ten healthy male subjects participated in the experiment. They were asked to extend their trunk by using the right and left erector spinae muscles during a sustained isometric contraction for twenty seconds. The force levels were 15%, 30%, 45%, 60%, and 75% of Maximal Voluntary Contraction (MVC), and the order of trials was randomized. The results showed that RMS was the best parameter to measure the force level of the muscle, and that the first coefficient of AR model was relatively sensitive parameter for the fatigue measurement at less than 60% MVC condition. At the 75% MVC, however, both MPF and the first coefficient of AR Model showed the best performance in quantification of muscle fatigue. Therefore, the sensitivity of measurement can be improved by properly selecting the parameter based upon the level of force during a sustained isometric condition.