Maxillofacial Plastic and Reconstructive Surgery

  • Volume 37
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  • Pages.46.1-46.6
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  • 2015
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  • 2288-8101(pISSN)
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  • 2288-8586(eISSN)
Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
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Early effect of Botox-A injection into the masseter muscle of rats: functional and histological evaluation

  • Moon, Young-Min (Department of Orthodontics, School of Dentistry, Dental Research Institute, Seoul National University) ;
  • Kim, Young-Jun (Department of Oral Medicine and Diagnosis, College of Dentistry, Research Institute of Oral Science, Gangneung-Wonju National University) ;
  • Kim, Min-Keun (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Kim, Seong-Gon (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Kweon, HaeYong (Sericultural and Apicultural Materials Division, National Academy of Agricultural Science) ;
  • Kim, Tae-Woo (Department of Orthodontics, School of Dentistry, Dental Research Institute, Seoul National University)
  • Received : 2015.11.11
  • Accepted : 2015.12.22
  • Published : 2015.12.31
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Abstract

Background: The purpose of this study was to evaluate the change of food intake after different dosages of botulinum toxin A (BTX) injection in the animal model. Additionally, the dimensional and histological change at 14 days after BTX injection was also evaluated. Methods: The comparative study was performed using the BTX injection model in rats (n = 5 for each group). Group 1 was the saline-injected group. Group 2 was the 5-unit BTX-injection group to each masseter muscle. Group 3 was the 10-unit BTX-injection group to each masseter muscle. Food intake rates and body weight were checked daily before and after BTX injection until 10 days. All animals were sacrificed at 14 days after BTX injection, and the specimens underwent hematoxylin and eosin stain and immunohistochemical staining for myosin type II (MYH2). Results: The recovery of food intake in groups 2 and 3 decreased significantly compared with group 1 from day 2 to day 7 and day 9 after injection (p < 0.05). The BTX-treated masseter muscles were significantly smaller than those in group 1 (p = 0.015). The immunohistochemical findings demonstrated that the expression of MYH2 was significantly higher in group 3 compared to groups 1 and 2 (p < 0.001). Conclusions: BTX injection to the masseter muscle in rats demonstrated short food-intake-rate reduction with recovery until 10 days after injection. The thickness of the masseter muscle and MYH2 expression were significantly changed according to the injected dose of BTX.

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References

  1. Kiris E, Burnett JC, Kane CD, Bavari S (2014) Recent advances in botulinum neurotoxin inhibitor development. Curr Top Med Chem 14(18):2044-2061 https://doi.org/10.2174/1568026614666141022093350
  2. Tan EK, Jankovic J (2000) Treating severe bruxism with botulinum toxin. J Am Dent Assoc 131:211-216 https://doi.org/10.14219/jada.archive.2000.0149
  3. Watts MW, Tan EK, Jankovic J (1999) Bruxism and cranial-cervical dystonia: is there a relationship? Cranio 17:196-201 https://doi.org/10.1080/08869634.1999.11746095
  4. Molina OF, Dos Santos JJ, Nelson SJ, Grossman E (1997) Prevalence of modalities of headaches and bruxism among patients with craniomandibular disorder. Cranio 15:314-325 https://doi.org/10.1080/08869634.1997.11746026
  5. Santamato A, Ianieri G, Ranieri M, Megna M, Panza F, Fiore P et al (2008) Botulinum toxin type A in the treatment of sialorrhea in Parkinson's disease. J Am Geriatr Soc 56:765-767 https://doi.org/10.1111/j.1532-5415.2008.01612.x
  6. Seok H, Park YT, KimSG PYW (2013) Correction of post-traumatic anterior open bite by injection of botulinum toxin type A into the anterior belly of the digastric muscle: case report. J Korean Assoc Oral Maxillofac Surg 39:188-192 https://doi.org/10.5125/jkaoms.2013.39.4.188
  7. Kim HJ, Yum KW, Lee SS, Heo MS, Seo K (2003) Effects of botulinum toxin type A on bilateral masseteric hypertrophy evaluated with computed tomographic measurement. Dermatol Surg 29:484-489
  8. Kim JH, Shin JH, Kim ST, Kim CY (2007) Effects of two different units of botulinum toxin type A evaluated by computed tomography and electromyographic measurements of human masseter muscle. Plast Reconstr Surg 119:711-717 https://doi.org/10.1097/01.prs.0000239453.67423.99
  9. von Lindern JJ, Niederhagen B, Appel T, Berge S, Reich RH (2001) Type A botulinum toxin for the treatment of hypertrophy of the masseter and temporal muscles: an alternative treatment. Plast Reconstr Surg 107:327-332 https://doi.org/10.1097/00006534-200102000-00004
  10. Ahn KY, Kim ST (2007) The change of maximum bite force after botulinum toxin type A injection for treating masseteric hypertrophy. Plast Reconstr Surg 120:1662-1666 https://doi.org/10.1097/01.prs.0000282309.94147.22
  11. Kolaski K, Ajizian SJ, Passmore L, Pasutharnchat N, Koman LA, Smith BP (2008) Safety profile of multilevel chemical denervation procedures using phenol or botulinum toxin or both in a pediatric population. Am J Phys Med Rehabil 87(7):556-566 https://doi.org/10.1097/PHM.0b013e31817c115b
  12. Park SY, Park YW, Ji YJ, Park SW, Kim SG (2015) Effects of a botulinum toxin type A injection on the masseter muscle: an animal model study. Maxillofac Plast Reconstr Surg 37:10 https://doi.org/10.1186/s40902-015-0010-8
  13. Tsai CY, Chiu WC, Liao YH, Tsaic CM (2009) Effects on craniofacial growth and development of unilateral botulinum neurotoxin injection into the masseter muscle. Am J Orthod Dentofacial Orthop 135(2):142, e1-6
  14. Kiliaridis S, Engstrom C, Thilander B (1988) Histochemical analysis of masticatory muscle in the growing rat after prolonged alteration in the consistency of the diet. Arch Oral Biol 33:187-193 https://doi.org/10.1016/0003-9969(88)90044-1
  15. Kim HC, Song JM, Kim CJ, Yoon SY, Kim IR, Park BS, Shin SH (2015) Combined effect of bisphosphonate and recombinant human bone morphogenetic protein 2 on bone healing of rat calvarial defects. Maxillofac Plast Reconstr Surg 37:16 https://doi.org/10.1186/s40902-015-0015-3
  16. Rafferty KL, Liu ZJ, Ye W, Navarrete AL, Nguyen TT, Salamati A, Herring SW (2012) Botulinum toxin in masticatory muscles: short-and long-term effects on muscle, bone, and craniofacial function in adult rabbits. Bone 50(3):651-662 https://doi.org/10.1016/j.bone.2011.11.015
  17. Tsai CY, Lin YC, Su B, Yang LY, Chiu WC (2012) Masseter muscle fibre changes following reduction of masticatory function. Int J Oral Maxillofac Surg 41(3):394-399 https://doi.org/10.1016/j.ijom.2011.10.016
  18. Lee CJ, Kim SG, Kim YJ, Han JY, Choi SH, Lee SI (2007) Electrophysiologic change and facial contour following botulinum toxin A injection in square faces. Plast Reconstr Surg 120:769-778 https://doi.org/10.1097/01.prs.0000271068.71399.ae
  19. Kim NH, Chung JH, Park RH, Park JB (2005) The use of botulinum toxin type A in aesthetic mandibular contouring. Plast Reconstr Surg 115:919-930 https://doi.org/10.1097/01.PRS.0000153236.79775.A0
  20. Freund B, Schwartz M, Symington JM (1999) The use of botulinum toxin for the treatment of temporomandibular disorders: preliminary findings. J Oral Maxillofac Surg 57:916-920 https://doi.org/10.1016/S0278-2391(99)90007-1
  21. Yu CC, Chen PKT, Chen YR (2007) Botulinum toxin A for lower facial contouring: a prospective study. Aesthetic Plast Surg 31:445-451 https://doi.org/10.1007/s00266-007-0081-8
  22. Matic DB, Yazdani A, Wells RG, LeeTY GBS (2007) The effects of masseter muscle paralysis on facial bone growth. J Surg Res 139:243-252 https://doi.org/10.1016/j.jss.2006.09.003
  23. Tsai CY, Yang LY, Chen KT, Chiu WC (2010) The influence of masticatory hypofunction on developing rat craniofacial structure. Int J Oral Maxillofac Surg 39:593-598 https://doi.org/10.1016/j.ijom.2010.02.011
  24. Kiliaridis S, Mejersjo C, Thilander B (1989) Muscle function and craniofacial morphology: a clinical study in patients with myotonic dystrophy. Eur J Orthod 11:131-138 https://doi.org/10.1093/oxfordjournals.ejo.a035975
  25. Tsai CY, Huang RY, Lee CM, Hsiao WT, Yang LY (2010) Morphologic and bony structural changes in the mandible after a unilateral injection of botulinum neurotoxin in adult rats. J Oral Maxillofac Surg 68(5):1081-1087 https://doi.org/10.1016/j.joms.2009.12.009
  26. Picard S, Lapointe NP, Brown JP, Guertin PA (2008) Histomorphometric and densitometric changes in the femora of spinal cord transected mice. Anat Rec (Hoboken) 291:303-307 https://doi.org/10.1002/ar.20645
  27. Poliachik SL, Bain SD, Threet D, Huber P, Gross TS (2010) Transient muscle paralysis disrupts bone homeostasis by rapid degradation of bone morphology. Bone 46:18-23 https://doi.org/10.1016/j.bone.2009.10.025
  28. Warner SE, Sanford DA, Becker BA, Bain SD, Srinivasan S, Gross T (2006) Botox induced muscle paralysis rapidly degrades bone. Bone 38:257-264 https://doi.org/10.1016/j.bone.2005.08.009
  29. Weiss A, Leinwand LA (1996) The mammalian myosin heavy chain gene family. Annu Rev Cell Dev Biol 12:417-439 https://doi.org/10.1146/annurev.cellbio.12.1.417
  30. Schiaffino S, Reggiani C (1996) Molecular diversity of myofibrillar proteins: gene regulation and functional significance. Physiol Rev 76:371-423 https://doi.org/10.1152/physrev.1996.76.2.371
  31. Gedrange T, Buttner C, Schneider M, Oppitz R, Harzer W (2005) Myosin heavy chain protein and gene expression in the masseter muscle of adult patients with distal or mesial malocclusion. J Appl Genet 46:227-236
  32. Harzer W, Worm M, Gedrange T, Schneider M, Wolf P (2007) Myosin heavy chain mRNA isoforms in masseter muscle before and after orthognathic surgery. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 104:486-490 https://doi.org/10.1016/j.tripleo.2007.01.017
  33. Raoul G, Rowlerson A, Sciote J, Codaccioni E, Stevens L, Maurage CA, Duhamel A, Ferri J (2011) Masseter myosin heavy chain composition varies with mandibular asymmetry. J Craniofac Surg 22(3):1093-1098 https://doi.org/10.1097/SCS.0b013e3182107766
  34. Rowlerson A, Raoul G, Daniel Y, Close J, Maurage CA, Ferri J, Sciote J (2005) Fiber-type differences in masseter muscle associated with different facial morphologies. Am J Orthod Dentofacial Orthop 127(1):37-46 https://doi.org/10.1016/j.ajodo.2004.03.025
  35. Borodic GE, Ferrante R, Pearce LB, Smith K (1994) Histologic assessment of dose-related diffusion and muscle fiber response after therapeutic botulinum A toxin injections. MovDisord 9:31-39
  36. Bobinac D, Malnar-Dragojevic D, Bajek S, Soic-Vranic T, Jerkovic R (2000) Muscle fiber type composition and morphometric properties of denervated rat extensor digitorum longus muscle. Croat Med J 41:294-297
  37. Del Gaudio JM, Sciote JJ (1997) Changes in myosin expression in denervated laryngeal muscle. Ann Otol Rhinol Laryngol 106:1076-1081 https://doi.org/10.1177/000348949710601212
  38. Gedrange T, Luck O, Hesske G, Buttner C, Seibel P, Harzer W (2001) Differential expression of myosin heavy-chain mRNA in muscles of mastication during functional advancement of the mandible in pigs. Arch Oral Biol 46:215-220 https://doi.org/10.1016/S0003-9969(00)00116-3
  39. Gedrange T, Gredes T, Spassov A, Mai R, Kuhn DU, Dominiak M et al (2013) Histological changes and changes in the myosin mRNA content of the porcine masticatory muscles after masseter treatment with botulinum toxin A. Clin Oral Investig 17(3):887-896 https://doi.org/10.1007/s00784-012-0750-0
  40. Cao R, Huang F, Wang P, Chen C, Zhu G, Chen L et al (2015) Chronic sleep deprivation alters the myosin heavy chain isoforms in the masseter muscle in rats. Br J Oral Maxillofac Surg 53(5):430-435 https://doi.org/10.1016/j.bjoms.2015.02.011
  41. Eriksson PO, Thornell LE (1983) Histochemical and morphological musclefibre characteristics of the human masseter, the medial pterygoid and the temporal muscles. Arch Oral Biol 28:781-795 https://doi.org/10.1016/0003-9969(83)90034-1

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