• Title/Summary/Keyword: Muscari

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High frequency somatic embryogenesis through leaf explant-derived callus culture in Muscari armeniacum cv. 'Early Giant' (무스카리 'Early Giant' 잎 절편 유래 캘러스 배양을 통한 고빈도 체세포배 발생)

  • Lee, Hyang-Bun;Jeon, Su-Min;Chung, Mi-Young;Han, Jeung-Sul;Kim, Chang-Kil;Lim, Ki-Byung;Chung, Jae-Dong
    • Journal of Plant Biotechnology
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    • v.39 no.1
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    • pp.69-74
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    • 2012
  • Using calli of $Muscari$ $armeniacum$ cv. 'Early Giant' that is monocotyledonous ornamental bulb crop with increasing demand in Korea, we carried out current studies to establish an in vitro multiple propagation protocol via somatic embryogenesis. We found that soft pale yellow green calli were induced from leaf explants cultured on all media containing 0.1~3.0 $mg{\cdot}L^{-1}$ auxins such as 1-naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). However, induced calli showed vigorous growth only when they further transferred on same media containing 2,4-D, 4-amino-3,5,6-tri-chloropicolinic acid (picloram), or 3,6-dichloro-o-anisic acid (dicamba). Although frequency of somatic embryo induction depended on callus source and PGR composition in somatic embryo induction media, somatic embryogenesis was initiated on surface of proliferated calli after transferring on media with no PGR or 0.01 $mg{\cdot}L^{-1}$ NAA co-supplemented with various cytokinins such as $N^6$-benzylaminopurine (BAP). Highest number of embryo at 9.3 per callus clump was obtained when calli which were grown under 0.1 $mg{\cdot}L^{-1}$ picloram supplementation were sub-cultured on medium with 0.01 $mg{\cdot}L^{-1}$ NAA and 0.5 $mg{\cdot}L^{-1}$ BAP. In addition, morphological characteristics of somatic embryo were categorized into following nine phases: globular, biased heart, biased torpedo, early cotyledonary, middle cotyledonary, late cotyledonary, early sprouting, middle sprouting, and late sprouting embryos.

Selecting Plants for the Extensive Rooftop Greening Based on Herbal Plants (초화류를 중심으로한 관리조방적 옥상녹화용 식물 소재 선정)

  • Lee, Eun-Heui;Cho, Eun-Jin;Park, Min-Young;Kim, Dong-Wook;Jang, Seong-Wan
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.10 no.2
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    • pp.84-96
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    • 2007
  • The purpose of this study was to select herbaceous plants that can be used for presenting various views and biodiversity on the extensive rooftop greening. Experiment plots were constructed in July 2005 on the rooftop of the Administration Building in the Seoul Women's University. For this experiment, planters were used to design rooftop greening. The size of a planter is 500mm${\times}$500mm${\times}$100mm and each planter has the water storage plate in the lower part of it. The soil was constructed by mixing pearlite, vermiculite, cocopeat, and leaf mold in the ratio of 6 : 2 : 1 : 1. The plot was divided into the watered plot and the dry plot. Since each plot was constructed 2 times, finally 4 planters were constructed in total. One hundred species were used for the experiment and 9 plants per species were planted in each planter. Plants were organized according to types of plants and the experiment used 86 native herbaceous plants, 6 herbs, and 8 foreign plants. The plots were monitored once a month, from July to November 2005. The length and width of plants were tape-measured and covering rate was calculated by CAD program. "SPSS 10.1" was used for a statistical analysis. The result showed no significant difference between the watered plots and the dry plots. In cases of some plants, there were statistically significant differences between the watered planter and dry planter such as follows : Astilbe chinensis and Polygonatum odoratum which are shade plants were measured as the highest value on the watered pots, and Aquilegia buergeriana, Chrysanthemum zawadskii, Calendula arvensis and Gypsophila cerastioides D.Don which are sunny plants were measured as the highest value on the dry plots. According to the final analysis of the data collected and observed for growth condition during the first year of the research, 51 species including Prunella vulgaris var. lilacina and Veronica linaiaefolia in native herbaceous plant, 5 species with Lavandula angustifolia in herbs, and 3 species with Lantana camara and Muscari armeniacum in foreign plants showed the highest growth condition. In conclusion, it is suggested that various plants including sedums could be effectively used for extensive rooftop greening to improve landscape(a view) of the rooftop and increase ecological values.

The Study of Vascular Plants in Hwangseong Park, Gyeongju-si (경주 황성공원의 관속식물상 연구)

  • You, Ju-Han;Kim, Young-Hun;Chen, Geng
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.38 no.3
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    • pp.35-49
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    • 2020
  • The purpose of this study is to provide the baseline data for conservation and management of the ecosystem of urban forest by surveying and analysing the vascular plants distributed in Hwangseong Park, Gyeongju-si. The results of the survey of vascular plants from April 2018 to May 2020 were summarized as 338 taxa including 78 families, 222 genera, 296 species, 5 subspecies, 18 varieties, 6 forms, 4 hybrids and 9 cultivars, and 110 taxa of planted species and 235 taxa of native species were identified. The representative planting species was Liriope muscari. The 7 taxa of rare plants and 5 taxa of Korean endemic plants were investigated. The specific plants by floristic region were totally 24 taxa, which were 1 taxa of grade V, 4 taxa of grade IV, 7 taxa of grade III, 4 taxa of grade II and 8 taxa of grade I. The 57 taxa of naturalized plants and 6 taxa of invasive alien plants were showed. In particular, Chionanthus retusus represent traditional landscape trees in Korea and have high ecological value. Therefore, the old trees of Chionanthus retusus will have to be managed by assigning a number for each individual. The invasive alien plants will need periodic control and development of programs that can work in conjunction with civic groups.