• 한국자원식물학회지
• /
• 제19권3호
• /
• pp.432-435
• /
• 2006

A rapid and mass propagation method for multiple shoots and plant regeneration using bulb scales of Muscari comosum var. plumosum were developed. In vitro different parts of bulb scale as explants were cultured on 11 kinds of MS (1962) media supplemented with various plant growth regulators to induce shoot and callus. A combination of 2.0 mg/L 6-BA and 2.0 mg/L IBA on MS medium was the most favorable and induced the highest production (80%) of shoot formation after 30 days. We also found that the middle part of bulb scale was the best for mass propagation of Muscari comosum var. plumosum of which production could reach 64.4%.

• 한국균학회지
• /
• 제45권1호
• /
• pp.68-73
• /
• 2017

In 2015, the leaves of broadleaf liriope, Liriope muscari, showing anthracnose symptoms were found in a field of Korea. The fungus was isolated and identified as Colletotrichum liriopes based on the morphological characteristics and nucleotide sequence data of the glyceraldehyde-3-phosphate dehydrogenase, internal transcribed spacer and actin genes. To the best of our knowledge, this is the first report of C. liriopes isolated from L. muscari in Korea.

• Current Research on Agriculture and Life Sciences
• /
• 제29권
• /
• pp.21-27
• /
• 2011

화경을 상, 중, 하부로 나누어 신초 및 자구를 유도하기 위하여 NAA와 몇 종류의 cytokinin 첨가 배지에서 각 부위별 화경 절편체를 배양해서 얻은 결과는 다음과 같다. 화경 조직에서 상부, 중부, 하부 중 소화경이 부착된 상부조직에서 다아체 및 자구 형성이 전반적으로 가장 양호 하였다. 다아체 형성은 NAA $0.1mg{\cdot}L^{-1}$와 TDZ $1.0mg{\cdot}L^{-1}$ 혼용배지에서 가장 효율적이었고 자구 형성은 NAA $0.01mg{\cdot}L^{-1}$와 kinetin $0.1mg{\cdot}L^{-1}$ 또는 NAA $0.01mg{\cdot}L^{-1}$와 kinetin $0.2mg{\cdot}L^{-1}$ 혼용배지에서 가장 효율적이었다. 온실에서 순화된 식물체는 정상적으로 개화하였으며 재식 2년차에 1.5개 내외의 자구를 가진 구를 생산할 수 있었다.

• 대한본초학회지
• /
• 제36권1호
• /
• pp.97-102
• /
• 2021

Objectives : Virus infection through the respiratory tract causes various inflammatory diseases such as pneumonia, cystic fibrosis, and obstructive pulmonary disease, causing enormous social damage. Therefore, it is very important to develop a treatment and prevention of infectious diseases. In this study, we investigated the effect of water extracts of Liriope muscari (WELM), known to improve lung function, on the inflammatory response of lung carcinoma cell line A549 cells induced by the viral double stranded RNA mimetic Polyinosinic:polycytidylic acid (Poly I:C). Methods : The cell viability by WELM treatment was analyzed using MTS assay in A549 cells. After inducing an inflammatory response to WELM-treated A549 cells with Poly I:C, the degree of apoptosis was confirmed through bright field microscopy. Interferon beta (IFN-β) mRNA expression level in A549 cells was analyzed by quantitative reverse transcription PCR (qRT-PCR). Results : WELM treatment has no significant effect on cell viability of A549 cells. We confirmed that pre-treatment of WELM effectively reduces the Poly I:C-induced apoptotic cell death in A549 cells. In addition, it was confirmed that the mRNA expression level of IFN-β, a pro-inflammatory cytokine increased by Poly I:C treatment, was significantly suppressed by WELM treatment in A549 cells. Conclusions : These results provide the evidence that WELM is effective at inhibiting inflammation on respiratory viral infections and suggest that Liriope muscari might be a valuable natural substance in the prevention and treatment of infectious diseases.

• 한국자원식물학회지
• /
• 제19권3호
• /
• pp.427-431
• /
• 2006

In vitro high-frequency plant regeneration of Muscari comosum var. plumosum through somatic embryogenesis was obtained via two developmental pathways: direct embryos and multiple shoots regenerated from embryogenic callus. Flower bud with pedicel, receptacle, petal and ovary wall, floral stalk and leaf as explants were cultured in MS medium supplemented with various plant growth regulators. Embryos formed directly from pedicel, receptacle and floral stalk. Depending on explant sources, the optimal medium was MS medium supplemented with 0.2 mg/L IBA and 0.3 mg/L BA, 3.0 mg/L IBA and 3.0 mg/L BA, and MS-free medium for pedicel, receptacle, and floral stalk, respectively. Multiple shoots regenerated from embryogenic cal]i which was initiated from petal, ovary and leaf were observed in MS medium with different concentrations and combinations of hormone. The most suitable medium for each type of explant was 3.0 mg/L IBA and 3.0 mg/L BA(petal and ovary) and 5.0 mg/L IBA and 5.0 mg/L BA (leaf) Furthermore, the combination of 0.1 mg/L 2,4-D and 1.0 mg/L BA was also good for all sources of explants not only for direct embryo formation, but also, for embryogenic callus induction.

• 화훼연구
• /
• 제18권4호
• /
• pp.261-265
• /
• 2010

본 연구는 우리나라 주요 재배종인 Muscari armeniacum 'Early Giant' 품종을 사용하여 엽절편체로부터 직접적으로 신초재생과 체세포배 발생에 미치는 생장조절제의 효과를 구명하였다. 무스카리의 엽조직으로부터 캘러스 과정을 거치지 않은 직접 신초형성은 2,4-D $0.1mg{\cdot}L^{-1}$가 함유된 배지에서 가장 좋았다. 반면, 체세포배 발생은 생장조절제를 첨가하지 않는 대조구와 IPA $0.1{\sim}1.0mg{\cdot}L^{-1}$가 함유된 농도의 배지에서 비교적 양호하였다. 무스카리의 엽조직으로부터 재생된 자구를 기외로 이식했을 때 맹아율은 모든 처리구에서 80%이상으로 높았으며 특히 NAA $0.1mg{\cdot}L^{-1}$, IPA $1.0{\sim}3.0mg{\cdot}L^{-1}$ 배지에서 재생된 자구의 생장이 양호하였다.

• 한국동물학회:학술대회논문집
• /
• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
• /
• pp.258.2-258
• /
• 1999

No Abstract, See Full Text

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2020년도 춘계학술대회
• /
• pp.67-67
• /
• 2020

Though there is an on-going need for the diversity of ornamental plants for in-door environment, their growth and stress adaptability in comparatively low light intensity condition require further studies for implementation. Here investigates the growth and chlorophyll fluorescence parameters of Farfugium japonicum (L.) Kitam, Liriope muscari (Decne.) L.H.Bailey and Acorus gramineus Aiton under several light intensities which were based on common in-door environment. The growth measurement of the plants included the quantity, length, width and SPAD value of the leaves. Calculated values of Fm/Fo, Fv/Fm, Pi_Abs, ETo/RC and DIo/RC were used as the parameters of the chlorophyll fluorescence under 10, 50, 100 and 200 PPFD (μmol·m^-2·s^-1) light intensities. In-door plants group was put in a closed room allowing no other light sources for 10 weeks and the control group was put in glass-greenhouse for the same period. The overall in-door growth of L. muscari was not significantly different in all light intensities compared to the control group and even showed the higher SPAD values. Also, an increasing tendency of Pi_Abs value under 10 to 100 PPFD was observed implying that L.muscari could adapt well to in-door environment. Measurement of A. gramineus growth mostly showed the highest values in the control group especially in the number of the leaves. Nevertheless, chlorophyll fluorescence parameters showed no significant value difference between in-door and the control groups and thus, A. gramineus might have possibility of successful adaptation to in-door environment. F. japonicum showed deficient growth in plant height and leaf length compared to the control but, it seemed to be able to sustain ornamental value under in-door light intensities. Furthermore, Pi_Abs and DIo/RC values were increased under in-door light conditions suggesting potential adaptability of F. japonicum.

• 화훼연구
• /
• 제19권1호
• /
• pp.49-54
• /
• 2011

본 연구는 우리나라 무스카리 우점품종인 'Early Giant'의 대량증식 체계를 구축하기 위하여 잎 절편으로부터 소인경 형성과 비대에 미치는 MS 무기염 농도, 당 농도 및 광조건의 영향을 조사하였다. 무스카리의 잎 절편을 2주간 암 배양 후 MS 기본배지에 NAA $0.01mg{\cdot}L^{-1}$, kinetin $0.2m{\cdot}L^{-1}$, 자당 $30g{\cdot}L^{-1}$와 gelrite $3g{\cdot}L^{-1}$가 첨가된 배지에 16시간 일장으로 명 배양하였을 때 자구형성 및 생육이 가장 양호하였다. 다만, 자구 비대는 MS 배지 내 $60g{\cdot}L^{-1}$ 당 농도에서 촉진되었다. 온실에서 순화된 식물체는 정상적으로 개화하였으며 정식 2년차에 1.5개 내외의 자구를 가진 구를 생산할 수 있었다.

• 생명과학회지
• /
• 제28권5호
• /
• pp.517-523
• /
• 2018

만성적 스트레스가 있는 상황에서, 과잉 생산된 cortisol은 glucocorticoid receptor (GR)를 활성화시킴으로써 해마(hippocampus)에 있는 신경 세포에 손상을 줄 수 있다. Cortisol을 생성하지 못하는 동물들의 경우, corticosterone이 스트레스 호르몬의 역할을 하는 것으로 알려져 있다. 한편, 인간의 경우, corticosterone은 aldosterone의 전구물질 이거나 cortisol과 비슷한 특성을 가지는 하나의 glucocorticoid로만 여겨져 왔다. 최근 인간을 대상으로 cortisol과 dexamethasone과 같은 합성 glucocorticoid의 기능에 관한 연구가 많이 이루어져 왔으나, corticosterone의 정확한 기능에 대하여 많이 알려져 있지 않다. 본 연구에서 corticosterone을 여러 농도로 SH-SY5Y 세포에 처리한 후 24시간과 48시간 때 viability를 조사한 결과, 높은 농도($500{\mu}M$과 $1,000{\mu}M$)에서 SH-SY5Y 세포의 성장 억제가 관찰된 반면, 낮은 농도($100{\mu}M$)에선 그 효과가 나타나지 않았다. 맥문동, 오미자, 복신 열수 추출물에 대한 세포 독성을 실시한 결과, 세 시료 모두 농도가 높아질수록 높은 세포 독성을 보였다. 한편, $500{\mu}g/ml$의 맥문동은 corticosterone에 의해 유도된 세포 성장 억제를 완화시켜 세포 성장을 회복시키는 효과를 보였다. 마지막으로, 맥문동 $500{\mu}g/ml$에 오미자와 복신의 농도를 달리하여 제조한 여러 혼합물의 시너지 효과를 알아본 결과, 대부분 혼합물이 약간의 효과를 보이긴 했으나, 음성 대조군 수준만큼 회복되지는 않았다.