• Parasites, Hosts and Diseases
• /
• v.39 no.2
• /
• pp.171-176
• /
• 2001

Western blot analysis was performed to diagnose vivax malaria using stage-specific recombinant antigens. Genomic DNA from the whole blood of a malaria patient was used as templates to amplify the coding regions for the antigenic domains of circumsporozoite protein (CSP-1), meroxoite surface protein (MSP-1), apical merozoite antigen (AMA- 1), serine repeat antigen (SERA), and exported antigen (EXP- 1) of Plasmodium vivax. Each amplified DNA fragment was inserted into a pGEX-4T plasmid to induce the expression of GST fusion protein in Escherichia coli by IPTG. The bacterial cell extracts were separated on 10% SDS-PAGE followed by western blot analysis with patient sera which was confirmed by blood smear examination. When applied with patient sera, 147 (91.9%) out of 160 vivax malaria, 12 (92.3%) out of 13 falciparum malaria, and all 9 vivax/falciparum mixed malaria reacted with at least one antigen, while no reactions occurred with 20 normal uninfected sera. In the case of vivax malaria, CSP-1 reacted with 128 (80.0%) sera, MSP-1 with 102 (63.8%), AMA-1 with 128 (80.0%), SERA with 115 (71.9%), and EXP-1 with 89 (55.6%), respectively. We obtained higher detection rates when using S antigens (91.9%) rather than using each antigen solely (55.6 - 80%), a combination of 2 (76.3 - 87.5%), 3 (85.6 - 90.6%), or 4 antigens (89.4 - 91.3%). This method can be applied to serological diagnosis, mass screening in endemic regions, or safety test in transfusion of prevalent vivax malaria.

• Journal of Energy Engineering
• /
• v.22 no.1
• /
• pp.28-37
• /
• 2013

Stirred tank is widely used in various industries for mixing operations and chemical reactions for single- or multi-phase fluid systems. For designing agitator of high performance, quantity data of internal flow characteristics influenced by mixing performance are definitely confirmed but quantity analysis about the transient flow characteristics of complicate structure is recognized as difficult problem in the present. In this study, two models of commercial CFD code Fluent 6.3 used to propose suitable for the tank analysis. Agitation of Stirred tank is analyzed using a mixed model and the flow in the stirred tank is analyzed using a standard k-${\varepsilon}$ model. Multiple reference frame(MRF) and Sliding mesh(SM), the analysis techniques were used For compare a result of CFD with a visualization experiment result, to grasp internal flow and mixing characteristic in stirred tank and to present fundamental analysis method.

• Journal of Microbiology and Biotechnology
• /
• v.17 no.11
• /
• pp.1826-1832
• /
• 2007

We describe the fabrication of poly(ethylene glycol) diacrylate (PEG-DA) hydrogel microstructures with a high aspect ratio and the use of hydrogel microstructures containing the enzyme ${\beta}$-galactosidase (${\beta}$-Gal) or glucose oxidase (GOx)/horseradish peroxidase (HRP) as biosensing components for the simultaneous detection of multiple analytes. The diameters of the hydrogel microstructures were almost the same at the top and at the bottom, indicating that no differential curing occurred through the thickness of the hydrogel microstructure. Using the hydrogel microstructures as microreactors, ${\beta}$-Gal or GOx/HRP was trapped in the hydrogel array, and the time-dependent fluorescence intensities of the hydrogel array were investigated to determine the dynamic uptake of substrates into the PEG-DA hydrogel. The time required to reach steady-state fluorescence by glucose diffusing into the hydrogel and its enzymatic reactions with GOx and HRP was half the time required for resorufin ${\beta}$-D-galactopyranoside (RGB) when used as the substrate for ${\beta}$-Gal. Spatially addressed hydrogel microarrays containing different enzymes were micropatterned for the simultaneous detection of multiple analytes, and glucose and RGB solutions were incubated as substrates. These results indicate that there was no cross-talk between the ${\beta}$-Gal-immobilizing hydrogel micropatches and the GOx/HRP-immobilizing micropatches.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2008.03a
• /
• pp.343-358
• /
• 2008

Piles of a bridge pier are connected with a column through a pile cap(footing). Behavior of the pile foundation can be different according to the connection method between piles and the pile cap. This difference causes a change of the design method. Connection methods between pile heads and the pile cap are divided into two groups ; rigid connections and hinge connections. KHBDC(Korea Highway Bridge Design Code) has specified to use rigid connection method for the highway bridge. In the rigid connection method, maximum bending moment of a pile occurs at the pile head and this helps the pile to prevent the excessive displacement. Rigid methods are also good to improve the seismic performance. However some specifications prescribe that conservative results through investigations for both the fixed-head condition and the free-head condition should be reflected in the design. This statement may induce an over-estimated design for the bridge which have very good quality structures with casing covered drilled shafts and the PC-house contained pile cap. Because the assumption of free-head conditions (hinge connections) are unreal for the elevated pile cap system with multiple piles of the long span sea-crossing bridges. On the other hand, elastic displacement method to evaluate the pile reactions under the pile cap is not suitable for this type of bridges due to impractical assumptions. So, full modeling techniques which analyze the superstructure and the substructure simultaneously should be performed. Loads and stress state of the very large diameter drilled shaft and the pile cap for Incheon Bridge which will the longest bridge in Korea were investigated through the full modeling for rigid connection conditions.

• Journal of Korean Elementary Science Education
• /
• v.31 no.3
• /
• pp.298-310
• /
• 2012

The purpose of this study was to analyze the characteristics of elementary school students' prediction changes by the suggestion types in a multiple anomalous situation. We investigated the responses, the rate and time of changing prediction, and cognitive conflicts of the students when repeated anomalous situation was suggested in experimental or logical way in science classes focused on buoyancy. As the anomalous situation was repeated, the students to change the prediction increased in number and also the rates to choose the correct prediction became higher. The group who was exposed in experimental way changed their prediction more than in logical way. In addition, when we classified the students to change the prediction by types, the group in experimental way showed higher rate of NM, MM type and FFT type. With anomalous situation repeated, cognitive conflicts of the students has been gradually declining in both groups. But it seemed that the group in experimental way experienced higher mental conflicts. In particular, as students changed the prediction more and arrived at the correct answer after changing their prediction, all the more so. It is concluded that the degree of students' changing prediction and experiencing cognitive conflict can be different according to the suggestion types for situation. Therefore the correlation with cognitive conflict factors can be also observed with the types of students' reactions.

• KSBB Journal
• /
• v.32 no.1
• /
• pp.16-21
• /
• 2017

The multiple sclerosis caused by multiple inflammatory disease or immune system disorder, is usually treated by interferon ${\beta}$ through adjusting the abnormal immune reactions. For high production of human interferon ${\beta}$ using recombinant E. coli, codon optimized and wild type genes were synthesized. When pET-15b or pET-21a vector was used as an expression vector with each gene, there was no target protein expression. When pQE30 vector was used as an expression vector, human interferon ${\beta}$ was expressed by recombinant E. coli XL1-blue and E. coli JM109. Using the codon optimized gene, the expression of human interferon ${\beta}$ was slightly increased as compared to that from wild type gene. However, most of expressed human interferon ${\beta}$ was insoluble form.

• Korean journal of dermatology
• /
• v.56 no.10
• /
• pp.609-613
• /
• 2018

Background: Psoriasis is a chronic inflammatory skin disease with an incidence of 0.5~3% of the worldwide population. The pathogenesis of psoriasis is related to dysregulated keratinocyte function and immune reactions. Notably, genetic factors are considered important etiological contributors. Globally, several researchers have recently performed genome-wide association studies (GWAS) to identify the genes related with psoriasis. Objective: We aimed to investigate the expression pattern of 2 candidate genes that were identified by GWAS. These include interleukin 28 receptor alpha (IL28RA) and CUB and Sushi multiple domains 1 (CSMD1). Methods: We applied imiquimod cream to develop a psoriasis-like mouse model and obtained skin tissue. We performed immunohistochemistry to detect the expression of IL-28A and CSMD1. Results: IL28RA expression increased at an early time point such as 1 day after the topical application of 5% imiquimod cream. However, its expression returned to baseline levels 2 weeks after the topical application of imiquimod cream. CSMD1 expression also increased after the topical application of imiquimod, with increased expression particularly observed in the upper epidermal layer. Notably, CSMD1 expression decreased 7 days after imiquimod cream application. Conclusion: These results suggest that IL28RA and CSMD1 may play key roles in the pathogenesis of psoriasis.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.6
• /
• pp.2635-2640
• /
• 2014

Sera of cancer patients may contain antibodies that react with a unique group of autologous cellular antigens called tumor-associated antigens (TAAs). The present study aimed to determine whether a mini-array of multiple TAAs would enhance antibody detection and be a useful approach in esophageal cancer detection and diagnosis. Our mini-array of multiple TAAs consisted of eleven antigens, p53, pl6, Impl, CyclinB1, C-myc, RalA, p62, Survivin, Koc, CyclinD1 and CyclinE full-length recombinant proteins. Enzyme-linked immunosorbent assays (ELISA) were used to detect autoantibodies against eleven selected TAAs in 174 sera from patients with esophageal cancer, as well as 242 sera from normal individuals. In addition, positive results of ELISA were confirmed by Western blotting. In a parallel screening trial, with the successive addition of antigen to a final total of eleven TAAs, there was a stepwise increase in positive antibody reactions. The eleven TAAs were the best parallel combination, and the sensitivity and specificity in diagnosing esophageal cancer was 75.3% and 81.0%, respectively. The positive and negative predictive values were 74.0% and 82.0%, respectively, indicating that the parallel assay of eleven TAAs raised the diagnostic precision significantly. In addition, the levels of antibodies to seven antigens, comprising p53, Impl, C-myc, RalA, p62, Survivin, and CyclinD1, were significantly different in various stages of esophageal cancer, which showed that autoantibodies may be involved in the pathogenesis and progression of esophageal cancer. All in all, this study further supports our previous hypothesis that a combination of antibodies might acquire higher sensitivity for the diagnosis of certain types of cancer. A customized mini-array of multiple carefully-selected TAAs is able to enhance autoantibody detection in the immunodiagnosis of esophageal cancer and autoantibodies to TAAs might be reference indicators of clinical stage.

• Food Science and Biotechnology
• /
• v.15 no.5
• /
• pp.664-671
• /
• 2006

Modeling the growth kinetics of lactic acid bacteria (LAB), one of the most valuable microbial groups in the food industry, has been actively pursued in order to understand, control, and optimize the relevant fermentation processes. Most modeling approaches have focused on the development of single population models. Primary single population models provide fundamental kinetic information on the proliferation of a primary LAB species, the effects of biological factors on cell inhibition, and the metabolic reactions associated with cell growth. Secondary single population models can evaluate the dependence of primary model parameters, such as the maximum specific growth rate of LAB, on the initial external environmental conditions. This review elucidates some of the most important single population models that are conveniently applicable to the LAB fermentation analyses. Also, a well-defined mixed population model is presented as a valuable tool for assessing potential microbial interactions during fermentation with multiple LAB species.

• Korean Institute of Interior Design Journal
• /
• v.20 no.6
• /
• pp.350-358
• /
• 2011

In contemporary architecture, Environment is not just a non-objective space. It is an experimental space where physical space and the principal result of interaction and order have been granted in symbolic, introverted space. Therefore, Yokohama International Port Terminal characteristics that appear in the architectural space, the nature of human desires and reveal the value of user-centered criteria to classify the type of response characteristics of the affordance are as follows; First, the experience response in the affordance reaction is when users reaction is multiple in theenvironment. Second, the evolution of organic reactions in a public space system is that it shows the spatial characteristics of the functional relationships. Third, the organic of space that can be seen through cultural practice in the inside and outside of the space could be explained as a perceptual response. It can be an opportunity to analyzed and develop in a various forms of space in environmental design if we understands the spatial characteristics of FOA's Yokohama International Port Terminal and applied end user spatial reaction analysis that shows an affordance relationship study.