• Title/Summary/Keyword: Multiple Calibration

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Prediction from Linear Regression Equation for Nitrogen Content Measurement in Bentgrasses leaves Using Near Infrared Reflectance Spectroscopy (근적외선 분광분석기를 이용한 잔디 생체잎의 질소 함량 측정을 위한 검량식 개발)

  • Cha, Jung-Hoon;Kim, Kyung-Duck;Park, Dae-Sup
    • Asian Journal of Turfgrass Science
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    • v.23 no.1
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    • pp.77-90
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    • 2009
  • Near Infrared Reflectance Spectroscopy(NIRS) is a quick, accurate, and non-destructive method to measure multiple nutrient components in plant leaves. This study was to acquire a liner regression equation by evaluating the nutrient contents of 'CY2' creeping bentgrass rapidly and accurately using NIRS. In particular, nitrogen fertility is a primary element to keep maintaining good quality of turfgrass. Nitrogen, moisture, carbohydrate, and starch were assessed and analyzed from 'CY2' creeping bentgrass clippings. A linear regression equation was obtained from accessing NIRS values from NIR spectrophotometer(NIR system, Model XDS, XM-1100 series, FOSS, Sweden) programmed with WinISI III project manager v1.50e and ISIscan(R) (Infrasoft International) and calibrated with laboratory values via chemical analysis from an authorized institute. The equation was formulated as MPLS(modified partial least squares) analyzing laboratory values and mathematically pre-treated spectra. The accuracy of the acquired equation was confirmed with SEP(standard error of prediction), which indicated as correlation coefficient($r^2$) and prediction error of sample unacquainted, followed by the verification of model equation of real values and these monitoring results. As results of monitoring, $r^2$ of nitrogen, moisture, and carbohydrate in 'CY2' creeping bentgrass was 0.840, 0.904, and 0.944, respectively. SEP was 0.066, 1.868, and 0.601, respectively. After outlier treatment, $r^2$ was 0.892, 0.925, and 0.971, while SEP was 0.052, 1.577, and 0.394, respectively, which totally showed a high correlation. However, $r^2$ of starch was 0.464, which appeared a low correlation. Thereof, the verified equation appearing higher $r^2$ of nitrogen, moisture, and carbohydrate showed its higher accuracy of prediction model, which finally could be put into practical use for turf management system.

Status and Prospect of Unmanned, Global Ocean Observations Network (글로벌 무인해양관측 네트워크 현황과 전망)

  • Nam, Sunghyun;Kim, Yun-Bae;Park, Jong Jin;Chang, Kyung-Il
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.19 no.3
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    • pp.202-214
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    • 2014
  • We introduce status and prospect of increasingly utilizing, unmanned, global ocean observing systems, and the global network to integrate, coordinate, and manage the systems. Platforms of the ocean observing system are diversified in order to resolve/monitor the variability occurring at multiple scales in both three-dimensional space and time. Here purpose, development history, and current status of the systems in two kinds - mobile (surface drifter, subsurface float, underwater glider) and fixed platforms (surface and subsurface moorings, bottom mounts), are examined and the increased future uses to produce synergies are envisioned. Simultaneous use of various mobile and fixed platforms is suggested to more effectively design the observing system, with an example of the NSF-funded OOI (Ocean Observations Initiative) program. Efforts are suggested 1) to fill the data gap existing in the deep sea and the Southern Ocean, and toward 2) new global network for oceanic boundary currents, 3) new technologies for existing and new sensors including biogeochemical, acoustic, and optical sensors, 3) data standardization, and 4) sensor calibration and data quality control.

Facial Expression Animation which Applies a Motion Data in the Vector based Caricature (벡터 기반 캐리커처에 모션 데이터를 적용한 얼굴 표정 애니메이션)

  • Kim, Sung-Ho
    • The Journal of the Korea Contents Association
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    • v.10 no.5
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    • pp.90-98
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    • 2010
  • This paper describes methodology which enables user in order to generate facial expression animation of caricature which applies a facial motion data in the vector based caricature. This method which sees was embodied with the plug-in of illustrator. And It is equipping the user interface of separate way. The data which is used in experiment attaches 28 small-sized markers in important muscular part of the actor face and captured the multiple many expression which is various with Facial Tracker. The caricature was produced in the bezier curve form which has a respectively control point from location of the important marker which attaches in the face of the actor when motion capturing to connection with motion data and the region which is identical. The facial motion data compares in the caricature and the spatial scale went through a motion calibration process too because of size. And with the user letting the control did possibly at any time. In order connecting the caricature and the markers also, we did possibly with the click the corresponding region of the caricature, after the user selects each name of the face region from the menu. Finally, this paper used a user interface of illustrator and in order for the caricature facial expression animation generation which applies a facial motion data in the vector based caricature to be possible.

Analysis of quercetin in extract of Hovenia dulcis Thunb (헛개나무과병추출물 중 퀘르세틴 분석)

  • Hu, SooJung;Oh, Jae-Myoung;Oh, Hyun-Suk;Moon, Myung-Hee;Bae, Yun-Young;Lee, Sung-Hye;Kim, Ji-An;Koo, Yong-Eui
    • Analytical Science and Technology
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    • v.29 no.6
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    • pp.255-260
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    • 2016
  • Based on the Korean Health Functional Food Act, health functional foods are dietary supplements containing nutrients or other substances that have nutritional or physiological effects. Since generic health functional food items have been expanded, this project was performed to develop a standardized analytical method of examining such sale items. The method exhibited high linearity in the quercetin calibration curve ($R^2$ > 0.999) over concentrations of $0-40{\mu}g/mL$. The limits of detection and quantitation for quercetin were $0.12{\mu}g/mL$ and $0.36{\mu}g/mL$, respectively, while quercetin recovery was 97.1-105.4 % with standard deviations of 1.15-3.11 %. To verify the accuracy of the analytical methods, the labeled amounts of purchased health functional foods were monitored. The recovery rate for multiple quercetin concentrations ranged from 82.5-105.1 % of the labeled amounts. Thus, the new method was suitable for all cases.

An Estimation of Flood Quantiles at Ungauged Locations by Index Flood Frequency Curves (지표홍수 빈도곡선의 개발에 의한 미 계측지점의 확률 홍수량 추정)

  • Yoon, Yong-Nam;Shin, Chang-Kun;Jang, Su-Hyung
    • Journal of Korea Water Resources Association
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    • v.38 no.1
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    • pp.1-9
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    • 2005
  • The study shows the possible use of the index flood frequency curves for an estimation of flood quantiles at ungauged locations. Flood frequency analysis were made for the annual maximum flood data series at 9 available stations in the Han river basin. From the flood frquency curve at each station the mean annual flood of 2.33-year return period was determined and the ratios of the flood magnitude of various return period to the mean annual flood at each station were averaged throughout the Han river basin, resulting mean flood ratios of different return periods. A correlation analysis was made between the mean annual flood and physiographic parameters of the watersheds i.e, the watershed area and mean river channel slope, resulting an empirical multiple linear regression equation over the whole Han river basin. For unguaged watershed the flood of a specified return period could be estimated by multiplying the mead flood ratio corresponding the return period with the mean annual flood computed by the empirical formula developed in terms of the watershed area and river channel slope. To verify the applicability of the methodology developed in the present study the floods of various return periods determined for the watershed in the river channel improvement plan formulation by the Ministry of Construction and Transportation(MOCT) were compared with those estimated by the present method. The result proved a resonable agreement up to the watershed area of approximately 2,000k $m^2$. It is suggested that the practice of design flood estimation based on the rainfall-runoff analysis might have to be reevaluated because it involves too much uncertainties in the hydrologic data and rainfall-runoff model calibration.

Method Development for the Profiling Analysis of Urine Globotriaosylceramide (Gb3) for the Screening of Fabry Disease by Tandem Mass Spectrometry (ESI-MS/MS를 이용한 소변 중 Globotriaosylceramide(Gb3)의 정량 및 임상 응용; 패브리병(Fabry) 진단)

  • Yoon, Hye-Ran;Cho, Kyung-Hee;Kang, Seung-Woo;Kwon, Young-Joo;Jeong, Choon-Sik;Lee, Yong-Soo
    • YAKHAK HOEJI
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    • v.51 no.2
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    • pp.96-102
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    • 2007
  • Measurement of globotriaosylceramide (Gb3, ceramide trihexoside) in urine has clinical importance for monitoring after enzyme replacement therapy in Fabry disease patients. The disease is an X-linked lipid storage disorder that results from a deficiency of the enzyme ${\alpha}$-galactosidase A (${\alpha}$-Gal A). The lack of ${\alpha}$-Gal A causes an intracellular accumulation of glycosphingolipids, mainly Gb3. A simple, rapid, and highly sensitive analytical method for Gb3 in urine was developed without labor-extensive pre-treatment by electrospray ionization MS/MS (ESI-MS/MS). Only simple 5-fold dilution of urine is necessary for the extraction and isolation of Gb3 in urine. Gb3 in diluted urine was dissolved in dioxane containing C17:0 Gb3 as an internal standard. After centrifugation it was directly injected and analyzed through guard column by in combination with multiple reaction monitoring mode of ESI-MS/MS. Eight isoforms of Gb3 were completely resolved from urine matrix. C24:0 Gb3 occupied 50% of total Gb3 as a major component in urine. Linear relationship for Gb3 isoforms was found in the range of 0.005${\sim}$5.0 ${\mu}$g/ml. The limit of detection (S/N=5) was 0.005 ${\mu}$g/ml and limit of quantification was 0.05 ${\mu}$g/ml for C24:0 Gb3 with acceptable precision and accuracy. Correlation coefficient of calibration curves for 8 Gb3 isoforms ranged from 0.9598 to 0.9975. This method could be useful for rapid and sensitive 1st line Fabry disease screening, monitoring and/or diagnostic tool for Fabry disease.

Bioequivalence of Atorva Tablet® to Lipitor Tablet® (Atorvastatin 20 mg) (리피토정® (아토르바스타틴 20 mg)에 대한 아토르바정®의 생물학적동등성)

  • Lim, Hyun-Kyun;Lee, Tae-Ho;Lee, Jae-Hyun;Youm, Jeong-Rok;Song, Jin-Ho;Han, Sang-Beom
    • Journal of Pharmaceutical Investigation
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    • v.38 no.2
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    • pp.135-142
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    • 2008
  • The present study describes the evaluation of the bioequivalence of two atorvastatin tablets, Lipitor $Tablet^{(R)}$ (Pfizer, reference drug) and Atorva $Tablet^{(R)}$ (Yuhan, test drug), according to the guidelines of Korea Food and Drug Administration (KFDA). Forty-nine healthy male Korean volunteers received each medicine at the atorvastatin dose of 40 mg in a $2{\times}2$ crossover study with a two weeks washout interval. After drug administration, serial blood samples were collected at a specific time interval from 0-48 hours. The plasma atorvastatin concentrations were monitored by an high performance liquid chromatography -tandem mass spectrometer (LC-MS/MS) employing electrospray ionization technique and operating in multiple reaction monitoring (MRM) and positive ion mode. The total chromatographic run time was 4.5 min and calibration curves were linear over the concentration range of 0.1-100 ng/mL for atorvastatin. The method was validated for selectivity, sensitivity, linearity, accuracy and precision. $AUC_t$ (the area under the plasma concentration-time curve from time zero to 48hr) was calculated by the linear log trapezoidal rule method. $C_{max}$ (maximum plasma drug concentration) and $T_{max}$ (time to reach $C_{max}$) were complied trom the plasma concentration-time data. Analysis of variance was carried out using logarithmically transformed $AUC_t$ and $C_{max}$. No significant sequence effect was found for all of the bioavailability parameters indicating that the crossover design was properly performed. The 90% confidence intervals of the $AUC_t$ ratio and the $C_{max}$ ratio for Atorva $Tablet^{(R)}$ / Lipitor $Tablet^{(R)}$ were ${\log}\;0.9413{\sim}{\log}\;1.0179$ and ${\log}\;0.831{\sim}{\log}\;1.0569$, respectively. These values were within the acceptable bioequivalence intervals of ${\log}\;0.8{\sim}{\log}\;1.25$. Based on these statistical considerations, it was concluded that the test drug, Atorva $Tablet^{(R)}$ was bioequivalent to the reference drug, Lipitor $Tablet^{(R)}$.

Analysis of Crop Survey Protocols to Support Parameter Calibration and Verification for Crop Models of Major Vegetables (주요 채소 작물 대상 작물 모형 모수 추정 및 검증을 지원하기 위한 생육 조사 프로토콜 분석)

  • Kim, Kwang Soo;Kim, Junhwan;Hyun, Shinwoo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.22 no.2
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    • pp.68-78
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    • 2020
  • Crop models have been used to predict vegetable crop yield, which would have a considerable economic impact on consumers as well as producers. A small number of models have been developed to estimate growth and yield of vegetables due to limited availability of growth observation data in high-quality. In this study, we aimed to analyze the protocols designed for collection of the observation data for major vegetable crops including cabbage, radish, garlic, onion and pepper. We also designed the protocols suitable for development and verification of a vegetable crop growth model. In particular, different measures were proposed to improve the existing protocol used by Statistics Korea (KOSTAT) and Rural Development Administration (RDA), which would enhance reliability of parameter estimation for the crop model. It would be advantageous to select sampling sites in areas where reliable weather observation data can be obtained because crop models quantify the response of crop growth to given weather conditions. It is recommended to choose multiple sampling sites where climate conditions would differ. It is crucial to collect time series data for comparison between observed and simulated crop growth and yield. A crop model can be developed to predict actual yield rather than attainable yield using data for crop damage caused by diseases and pests as well as weather anomalies. A bigdata platform where the observation data are to be shared would facilitate the development of crop models for vegetable crops.

Determination of Nitrovin in Fishery Products by Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS를 이용한 수산물 중 니트로빈의 정량분석법 개발 및 검증)

  • Kim, Joohye;Shin, Dasom;Kang, Hui-Seung;Jeong, Jiyoon;Rhee, Gyu-Seek
    • Journal of Food Hygiene and Safety
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    • v.33 no.2
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    • pp.118-123
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    • 2018
  • The objective of this study was to develop a sensitive method for the identification and determination of nitrovin in fishery products by using a solid-phase extraction (SPE), as performed with a liquid chromatography-tandem mass spectrometry (LC-MS/MS). The samples were extracted with a mixture of acetonitrile and water, and were then defatted with acetonitrile saturated hexane, after which further clean-up was accomplished with SPE on the hydrophilic-lipophilic balance (HLB) cartridges. The analytes were subsequently ionized in the positive mode of an electrospray ionization (ESI), and where thereby detected in a process of multiple reaction monitoring (MRM). The linearity (expressed as correlation coefficients) of the matrix calibration curves was > 0.985. The limit of the quantification for the nitrovin was measured at 0.001 mg/kg. The accuracy (expressed as average recovery) was noted between 72.1 and 122%. The precision (expressed as coefficient variation) was noted from 2.9 to 16.9%. According to the CODEX CAC/GL-71 guideline accuracy, precision, linearity, and limit of detection were determined in three matrices (which were flatfish, eel and shrimp). The proposed method was suitable for analyzing the associated nitrovin residues. This application and result can also be a factor to contribute to the non-detection drugs management in fishery products.

Comparison and Analysis of Matching DEM Using KOMPSAT-3 In/Cross-track Stereo Pair (KOMPSAT-3 In/Cross-track 입체영상을 이용한 매칭 DEM 비교 분석)

  • Oh, Kwan-Young;Jeong, Eui-Cheon;Lee, Kwang-Jae;Kim, Youn-Soo;Lee, Won-Jin
    • Korean Journal of Remote Sensing
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    • v.34 no.6_3
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    • pp.1445-1456
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    • 2018
  • The purpose of this study is to compare the quality and characteristics of matching DEMs by using KOMPSAT-3 stereo pair capture in in-track and cross-track. For this purpose, two stereo pairs of KOMPSAT-3 were collected that were taken in the same area. The two stereo pairs have similar stereo geometry elements such as B/H, convergence angle. Sensor modeling for DEM production was performed with RFM affine calibration using multiple GCPs. The GCPs used in the study were extracted from the 0.25 m ortho-image and 5 meter DEM provided by NGII. In addition, matching DEMs were produced at the same resolution as the reference DEMs for a comparison analysis. As a result of the experiment, the horizontal and vertical errors at the CPs indicated an accuracy of 1 to 3 pixels. In addition, the shapes and accuracy of two DEMs produced in areas where the effects of natural or artificial surface land were low were almost similar.