• Journal of Pharmaceutical Investigation
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• v.41 no.2
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• pp.95-102
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• 2011

An amphiphilic cationic branched methoxy poly (ethylene glycol)-branched polyethylenimine - poly(L-phenylalanine) (mPEG-bPEI-pPhe) block copolymer was successfully synthesized by ring-opening polymerization (ROP) of N-carboxyanhydride of L-phenylalanine (Phe-NCA) with mPEG-bPEI for the preparation of more stable polyelectrolyte complex (PEC) included a hydrophobic interaction. mPEG-bPEI was firstly prepared by the coupling of mPEG and bPEI using hexamethylene diisocyanate (HMDI). The structural properties of mPEG-bPEI-pPhe copolymers were confirmed by $^1H$ NMR. The copolymers exhibited a self-assemble behavior in water above critical aggregate concentration (CAC) in the range of 0.01-0.14 g/L. The CAC of copolymers obviously depended on the hydrophobic block content in the copolymers (the value decreased with the increase of the pPhe block content). The cationic copolymers have the ability to form multi-interaction complex (MIC) with bovine serum albumin (BSA) and plasmid DNA through multi-interaction (electrostatic and hydrophobic interaction). The physicochemical characterization of the complex was carried out by the measurement of zeta potential and particle size. Their zeta-potentials were positive (approximately +10 mV) and their sizes decreased with increasing pPhe contents in the copolymers (PPF/BSA wt% ratio = 2). The complex showed good stability at high ionic strength. Therefore, mPEG-bPEI-pPhe block copolymer was considered as a potential material to enhance the stability of complex including biotherapuetic drugs.

• Structural Engineering and Mechanics
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• v.41 no.4
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• pp.559-573
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• 2012

An adaptive modeling and simulation technique is introduced for the effective and reliable fluid-structure interaction analysis using MSC/Dytran for large-scale complex pressurized liquid containment. The proposed method is composed of a series of the global rigid sloshing analysis and the locally detailed fluid-structure analysis. The critical time at which the system exhibits the severe liquid sloshing response is sought through the former analysis, while the fluid-structure interaction in the local region of interest at the critical time is analyzed by the latter analysis. Differing from the global coarse model, the local fine model considers not only the complex geometry and flexibility of structure but the effect of internal pressure. The locally detailed FSI problem is solved in terms of multi-material volume fractions and the flow and pressure fields obtained by the global analysis at the critical time are specified as the initial conditions. An in-house program for mapping the global analysis results onto the fine-scale local FSI model is developed. The validity and effectiveness of the proposed method are verified through an illustrative numerical experiment.

• The Journal of Society for e-Business Studies
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• v.12 no.3
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• pp.1-17
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• 2007

A virtual enterprise (VE) can be mapped into a multi-agent system (MAS) that consists of various agents with specific role(s), communicating with each other to accomplish common goal(s). However, a MAS for enacting VE requires more advanced mechanism such as context that can guarantee autonomy and dynamism of VE members considering heterogeneity and complex structure of them. This paper is to suggest a context-based MAS as a platform for constructing and managing virtual enterprises. In the Context-based MAS a VE is a collection of Actor, Interaction (among Actors), Actor Context, and Interaction Context. It can raise the speed and correctness of decision-making and operation of VE enactment using context, i.e., information about the situation (e.g., goal, role, task, time, location, media) of Actors and Interactions, as well as simple data of their properties. The Context-based MAS for VE we proposed('VECoM') may consists of Context Ontology, Context Model, Context Analyzer, and Context Reasoner. The suggested approach and system is validated through an example where a VE tries to find a partner that could join co-development of new technology.

• Nuclear Engineering and Technology
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• v.55 no.1
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• pp.373-377
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• 2023

The Monte Carlo (MC) method has become an indispensable part of the nuclear radiation research field. Several widely used and well-known MC packages were developed for simulation of radiation transport and interaction with matter. All these MC packages require users to prepare an input script. The input script can become lengthy for complex models. The process of preparing these input scripts is time-consuming and error-prone. In the present work, we have developed an open-source GUI computer program for modelling radiation transport and interaction in multi-segmented slab phantoms using grid-based system for the widely used PHITS MC package. The developed tools would be useful for future users of PHITS MC package and particularly inexperienced users. The present program is distributed under GPL license and all users can freely download, modify and redistribute the program without any restrictions.

• BMB Reports
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• v.29 no.3
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• pp.230-235
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• 1996

The interaction of ${\alpha}-ketoglutarate$ dehydrogenase complex (${\alpha}-KGDC$) with a hydrophobic fluorescent probe [1,1'-bi(4-aniline)naphthalene-5,5'-disulfonic acid] (bis-ANS) was studied. The punfied ${\alpha}-KGDC$ was potently inhibited by bis-ANS with an apparent half maximal inhibitory concentration ($IC_{50}$) of 9.8 ${\mu}m$ at pH 8.0. The catalytic activities of both the E1o and E2o subunits were predominantly inhibited while that of the E3 component was hardly affected. The binding of bis-ANS to the enzyme caused a marked enhancement and blue shift from 523 nm to 482 nm in the fluorescence emission spectrum. The dissociation constant ($K_d$) and the number of binding sites (n) were calculated to be 0.87 mM and 158, respectively. Allosteric regulators such as purine nucleotides and divalent cations further increased the fluorescence intensity of the $bis-ANS-{\alpha}-KGDC$ binary complex. These data suggest that the binding of these allosteric regulators to ${\alpha}-KGDC$ may cause the conformational changes in the enzyme and that bis-ANS could be used as a valuable probe to study the interaction of the multi-enzyme complex and its allosteric regulators.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2009.05a
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• pp.289-292
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• 2009

Injector dynamics of multi swirl injectors in an annular combustor have been investigated by LES(Large Eddy Simulation) turbulent model with MPI parallel computation technique. The present study employs the LM6000 lean premixed swirl-stabilized annular combustor. Real shape combustor is simulated in order to investigate the detail interaction mechanism among multi-injectors. The strong vortex breakdown occurs at the impinging surface between the adjacent injectors so that the complex and strong oscillatory pressure propagates inside of the combustor. Tangential pressure fluctuation mode was captured by including multi injectors in computational domain.

• Journal of Microbiology and Biotechnology
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• v.31 no.8
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• pp.1183-1189
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• 2021

Autodisplay of a multimeric protein complex on a cell surface is limited by intrinsic factors such as the types and orientations of anchor modules. Moreover, improper folding of proteins to be displayed often hinders functional cell surface display. While overcoming these drawbacks, we ultimately extended the applicability of the autodisplay platform to the display of a protein complex. We designed and constructed a cell surface attachment (CSA) system that uses a non-covalent protein-protein interaction. We employed the high-affinity interaction mediated by an orthogonal cohesin-dockerin (Coh-Doc) pair from Archaeoglobus fulgidus to build the CSA system. Then, we validated the orthogonal Coh-Doc binding by attaching a monomeric red fluorescent protein to the cell surface. In addition, we evaluated the functional anchoring of proteins fused with the Doc module to the autodisplayed Coh module on the surface of Escherichia coli. The designed CSA system was applied to create a functional attachment of dimeric α-neoagarobiose hydrolase to the surface of E. coli cells.

• 한국전산유체공학회:학술대회논문집
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• 2008.03a
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• pp.57-70
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• 2008

A high-order accurate Euler flow solver based on a discontinuous Galerkin finite-element method has been developed for the numerical simulations of blade-vortex interaction phenomena on unstructured meshes. A free vortex in freestream was investigated to assess the vortex-preserving property and the accuracy of the present flow solver. Blade-vortex interaction problems in subsonic and transonic freestreams were simulated by adopting a multi-level solution-adaptive dynamic mesh refinement/coarsening technique. The results were compared with those of other numerical and experimental methods. It was shown that the present discontinuous Galerkin flow solver can preserve the vortex structure for significantly longer vortex convection time and can accurately capture the complex unsteady blade-vortex interaction flows, including generation and propagation of acoustic waves.

• 한국전산유체공학회:학술대회논문집
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• 2008.10a
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• pp.57-70
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• 2008

A high-order accurate Euler flow solver based on a discontinuous Galerkin finite-element method has been developed for the numerical simulations of blade-vortex interaction phenomena on unstructured meshes. A free vortex in freestream was investigated to assess the vortex-preserving property and the accuracy of the present flow solver. Blade-vortex interaction problems in subsonic and transonic freestreams were simulated by adopting a multi-level solution-adaptive dynamic mesh refinement/coarsening technique. The results were compared with those of other numerical and experimental methods. It was shown that the present discontinuous Galerkin flow solver can preserve the vortex structure for significantly longer vortex convection time and can accurately capture the complex unsteady blade-vortex interaction flows, including generation and propagation of acoustic waves.

• Journal of the Society of Disaster Information
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• v.11 no.4
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• pp.542-547
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• 2015

This study is discussing with respect to the access sector for terrorism response based on the thinking and methodology of complex systems theory, which is mainly used in many disciplines today to effectively respond to complex multi-environment change its purpose there. As a result, Butterfly Effect, fractal & self-similarity, self-organization, emergence, coevolution, edge of chaos the applicability of the corresponding field of terrorism through the complex system theory as metaphorical will be able to navigate.