• 대한의용생체공학회:의공학회지
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• 제27권6호
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• pp.409-417
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• 2006

Research on neural circuit is a difficult area due to complexity and inaccessibility. Due to recent developments, the research using multi-electrode array of cells or tissues has become an important research area. However, there are some difficulties to decode the submerged meaning from huge and complex neural data. Moreover, it needs a harmonic collaboration between informatics and bioscience. In this paper, we have developed a custom-designed signal processing technique for multi-electrode array measured neural responses induced by electrical stimuli to the hippocampal tissue slices of the rat brain. The raw data from hippocampal slice using the multi-electrode array system were saved in a computer. Then we estimated characteristic points in each channel and calculated the total activity. To estimate the points, we used the Polynomial Fitting Approximation Method. Using the calculated total activity, we could provide the histogram or pseudo-image matrix to help interpretation of results.

• 대한전기학회논문지:시스템및제어부문D
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• 제51권9호
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• pp.423-430
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• 2002

The fabrication and experimentation of multi-channel electrodes which enable detecting and recording of multi-site neuronal signals have been investigated. A multi-channel electrode array was fabricated by depositing 2000${\AA}$ thick Au layer on the 1000${\AA}$ thick Ti adhesion layer on a glass wafer. The metal paths were patterned by wet etching and passivated by depositing a PECVD silicon nitride insulation layer to prevent signals from intermixing or cross-talking. After placing a thin slice of rat cerebellar granule cell in the culture ring located in central portion of the multi-channel electrode plate, a neuronal signal from an electrode which is in contact with the cerebellar granule cell has been detected. It was found that the electrode impedance ranges 200㏀∼1㏁ and the impedance is not changed by cleaning with nitric acid. Also, the impedance is inversely proportion to the exposed electrode area and the cross-talk is negligible when the electrode spacing is bigger than 600$\mu\textrm{m}$. The amplitude and frequency of the measured action potential were 38㎷ and 2㎑, which are typical values. From the experimental results, the fabricated multi-channel electrode array proved to be suitable for multi-site neuronal signal detection for the analysis of a complicated cell network.

• 재활복지공학회논문지
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• 제10권4호
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• pp.279-285
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• 2016

신경보철 의수를 제어하기 위해서는 말초 신경신호를 측정하고 분석함으로써 사용자의 동작의도를 인식해야 한다. 본 논문에서는 다채널 신경전극 어레이, 전극 삽입 장치, 신경신호 증폭기로 구성된 말초 신경신호 측정 시스템을 제안한다. 마취상태에서 전극 삽입 장치를 이용하여 16 채널의 신경전극 어레이를 백서의 좌골신경에 안정적으로 삽입하였다. 수동적 관절 운동을 유도하거나 기계적 피부 자극을 인가하였을 때, 신경신호 증폭기를 통해 근육 또는 피부 감각 신경신호가 측정됨을 확인하였다. 또한, 개별 신경 단위를 분리하기 위해 활동전위 분류방법을 제안한다. 근육의 내부 수용기들은 근방추와 골지건 기관 수용기로 구분되었으며, 피부의 외부 수용기들은 완만적응과 급속적응 수용기로 분리되었다. 이와 같은 실험결과는 제안한 방법이 신경보철 시스템에서 말초 신경신호의 측정과 분석에 적용될 수 있음을 보였다.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2003년도 가을 학술발표회 논문집
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• pp.663-666
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• 2003

Using numerical analysis modeling of Multi-electrode Array that could be applied to the corrosion state measurement of a reinforcing steel bar in concrete, the steel bar location inside concrete can be probed by the investigation of the change of the measured impedance from concrete surface determined by the electrical impedance of interface between the steel bar and concrete, the electrical resistivity of concrete, the array of electrodes and the relative location and diameter of the steel bar.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2004년도 추계학술대회 논문집 Vol.17
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• pp.481-484
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• 2004

다중 채널 전극 위에 세포를 성장시켜 전극면을 통해 검출되는 신경 신호의 손실을 줄이고 주파수의 변형을 줄이기 위해서 전극과 전해질의 사이의 impedance를 줄이는 것이 바람직하다. 전하 이동을 증대시키기 위해서는 낮은 impedance가 요구되며 이를 위한 전극의 개선 방안으로 전극면이 증착될 기판의 표면을 거칠게 하여 결과적으로 전극면의 표면적을 넓히는 방법을 모색하였다. 기판으로 사용되는 glass(Pyrex#7740)의 구성 물질 중에서 4%를 차지하는 $N^+$ 이온을 황산 용액으로 표면 처리하여 제거함으로써 매끈한 표면을 거칠게 하여 표면적을 넓힐 수 있다. 기판으로 사용되는 glass (pyrex#7740) $1cm{\times}1cm{\times}0.05cm$를 50%, 95% 농도의 황산 용액 내에서 각각 30분, 60분 동안 상온에서 표면처리를 진행하였다. AFM을 이용하여 표면을 관찰한 결과 황산 용액 95%에서 30분간 표면 처리를 진행한 시편에서 최대 $4000{\AA}$정도의 조도를 얻었다. 이후 동일 시편에 대해 전극으로 사용될 Ti/Au를 각각 $500{\AA}/2500{\AA}$ 증착 후 사진식각 공정으로 MEA(Multi-channel electrode array)를 제작하여 impedance를 측정한 결과, 표면 처리 후 impedance가 70% 개선되었음을 측정하였다.

• 대한의용생체공학회:의공학회지
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• 제36권5호
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• pp.221-227
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• 2015

In this study, Multi-Array Electrodes (MAE) and Programmable Multi-channel Electrical Stimulator (PMES) were implemented for firing Trigger Points (TPs) of the patient with Myofascial Pain Syndrome (MPS). MAE has 25 Ag/AgCl electrodes arranged in the form of array ($5{\times}5$) fabricated with flexible pad, which are applicable to be easy-attached to curved specific region of the human body. PMES consisted of 25 channels. Each channel was to generate various electric stimulus patterns (ESPs) by changing the mono-phasic or bi-phasic of ESP, On/Off duration of ESP, the interval between ESP, and amplitude of ESP. PMES hardware was composed of Host PC, Stimulation Pattern Editing Program (SPEP), and Multi-channel Electrical Stimulator (MES). Experiments were performed using MAE and PMES as the following. First experiment was performed to evaluate the function for each channel of Sub- Micro Controller Unit (SMCU) in MES. Second experiment was conducted on whether ESP applied from each channel of SMCU in PMES was focused to the electrode set to the ground, after applying ESP being output from each channel of SMCU in PMES to MAE.

• 전기전자학회논문지
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• 제22권1호
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• pp.149-155
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• 2018

본 논문에서는 전기영상법(ET)을 이용하여 수직 다층구조 대지저항률을 영상복원 하였다. 일반적으로 접지 시공을 위한 대지 분석은 수평 다층 구조라는 가정 하에 행해지지만, 현실적으로는 지하구조가 수직 구조일 경우도 발생하게 된다. 여기에서는 수직 다층구조의 대지분석에 유리한 전극배열법을 찾아내기 위하여 전기영상법에 가장 널리 사용되는 Wenner, Schlumberger 그리고 쌍극자배열을 테스트하였다. ET영상복원에는 Gauss-Newton 역산 알고리즘이 이용되었다. RMS 오차 분석결과를 보면 Wenner 배열의 경우가 RMS 오차가 가장 적게 나타나는 것을 확인할 수 있었다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
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• pp.372.1-372.1
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• 2016

Recently, there are a lot of diseases all around the world. Out of them, Atherosclerosis (AS) is the most common cause of stroke, cardiovascular mortality, and myocardial infarction. The macrophage-derived foam cell, which is formed by oxidized low-density lipoprotein (oxLDL), is the crucial marker for AS. In this study, we report a label-free capacitance imaging technique with multi-electrode array (MEA). The lipid-rich aorta arch lesions, which are derived from an apolipoprotein-E receptor-deficient (apoE-/-) mouse, exhibit higher capacitance than the lipid-free aorta arch, allowing the capacitance imaging of lipid region in atherosclerosis. To improve the contacts between MEA and tissue, polypyrrole(PPy)-coated multi walled carbon nanotubes (MWNTs) multi electrode array (PPy-MWNTs-MEA) was fabricated. Compared to TiN-MEA, PPy-MWNTs-MEA yielded lower contact impedance and better capacitance images. In addition, we have also developed a flexible MEA using single walled carbon nanotubes on a PET substrate. The lipid region could be discriminated in the capacitance images of the lipid-rich aorta arch lesions measured using flexible MEA, demonstrating a feasibility of in vivo applications.

• Journal of Semiconductor Engineering
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• 제1권3호
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• pp.88-98
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• 2020

As biosensors are widely used in the medical field, flexible devices compatible with live animals have aroused great interest. Especially, significant research has been carried out to develop implantable or skin-attachable devices for real-time bio-signal sensing. From the device point of view, various biosensor types such as field-effect transistors (FETs) and multi-electrode arrays (MEAs) have been reported as diverse sensing strategies. In particular, the flexible FETs and MEAs allow semiconductor engineering to expand its application, which had been impossible with stiff devices and materials. This review summarizes the state-of-the-art research on flexible FET and MEA biosensors focusing on their materials, structures, sensing targets, and methods.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2002년도 추계학술대회 논문집 전기물성,응용부문
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• pp.257-259
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• 2002

In investigating the characteristics of a neural network, the use of planar microelectrode array shows several advantages over normal intracellular recording[1]. A transparent indium tin oxide(ITO) multi-electrode array(MEA) was fabricated and its top surface was insulated with photodefinable polyimide(HD-8001) except the exposed area for interfacing between the ITO electrodes and the neuronal cells. The exposed ITO electrodes were platinized in order to reduce the impedance between the electrodes and electrolyte. The one-minute platinization with $0.99nA/{\mu}m^2$ current density reduced the average impedance of the electrodes from $2.5M\Omega\;to\;90k\Omega$ at 1kHz in normal ringer solution. Cardiac cells were cultured on this MEA as a pilot study before neuron culture. The signals detected by the platinized electrodes had larger amplitudes and improved signal to noise ratio(SNR) compared to non-platinized electrodes. It is clear that microelectrodes need to have lower impedance to make reliable extracellular recordings, and thus platinization is essential part of MEA fabrication. Burst spike of cultured olfactory bulb was also detected with the MEA having platinized electrodes.