• 한국잠사곤충학회지
• /
• 제47권2호
• /
• pp.68-77
• /
• 2005

아열대산 MK-T2의 생태적 특성조사, 발근 특성 그리고 오디의 형태와 이화학적 특성, 사료가치 검정 평가, MK-T2로 사육한 5령3일차 누에의 아미노산 분석과 누에 에탄올 추출액의 약리효과에 대한 연구를 하여 다음과 같은 결과를 얻어 잎과 오디 등 다용도 $\ulcorner$청강상(淸江桑)$\lrcorner$ 자체개발 품종육성의 기본자료로 활용하였다. 1. 아열대산 MK-T2의 생태적 특성을 조사한 결과 발아 개엽기는 개량뽕과 비교하여 $9{\sim}10$일 빠르며 새순의 발육 또한 67.2 cm로 3 cm 정도 길었으며, 새순의 뽕잎수도 18.6 개로 2개가 많았다. 2. 엽의 형태는 엽장이 엽폭보다 약간 발달한 1.10 비율을 나타내었으며 엽두께, 엽면적은 각각 $228.2{\mu}m,\;225.6cm^2$로서 개량뽕보다 약간 얇거나 작은편이었다. 3. MK-T2는 화주부분이 0.7mm로 짧게 존재하며 주두내면에 드물게 미모(微毛)가 분포하여 장화계구(長花桂區) 계두유모류(桂頭有毛類)에 속한다고 판단이 된다. 4. 고손장비율은 5.7%로서 개량뽕의 12.9%와 비교하여 상당히 우수하지만 무농약재배의 조건에서 충해의 경우 주당 피해율이 88.6%로서 개량뽕의 47.5%의 거의 2배정도 약함을 보여주었다. 5 .무처리 신소삽목시 발근율이 가지의 굵기와 무관하게 100% 발근하여 온대지역의 개량뽕이 15mm 이상에서 10% 발근한 사실과 비교하여 매우 우수한 발근력을 보여주었으며 발근 형태적 특성은 토양과 물 모두 45%비율로 가지의 중부와 하부에서 발근하는 특성을 보여주어 하부에서 발근하는 온대지역의 뽕나무와는 많은 차이를 나타내었다. 6. MK-T2의 오디는 길이가 24.6 mm, 수분율 78.8%, 당도가 19.21 Brix%로 오디용으로 개발된 품보 20, 검설뽕과 비슷한 성적을 나타내었으나 특이하게 pH 4.7로 단맛과 신맛을 함께 지니고 있었다. 7. NK-T2의 사료가치 검정평가 결과 개량뽕과 비교하여 유충기간과 화용비율, 견충비율은 거의 비슷하였으나, 단견중, 견층중. 2만두 수견량은 약간 떨어지는 결과를 보여 엽질이 비교적 우량하다고 평가되었다. 8. MK-T2로 사육한 5령3일차 누에의 아미노산을 분석한 결과 개량뽕을 급이한 대조구 간에는 서로 거의 유사한 아미노산 조성을 보여주었으나 MK-T2를 급이한 시험구의 경우 대조구와 비교하여 Leu에서 차이를 보여주었으며 Ile는 대조구에서는 검출되지 않았으나 MK-T2 시험구에서는 검출되었다. 9. MK-T2로 사육한 5령3일차 누에 에탄올 추출물의 약리효과 검정결과 누에 에탄을 추출물을 투여한 쥐에서 적출한 신장조직에 대한 HE 염색 및 조직면역화학염색 모두에서 조직학적 병징이 나타나지 않았고 $TGF-{\beta}1$ 단백 발현이 거의 확인되지 않는 수준으로 낮아졌다.

• 한국잠사곤충학회지
• /
• 제25권2호
• /
• pp.37-43
• /
• 1984

누에병 중에 피해가 막대한 전염성 연화병을 방제하기 위하여 바이러스 증식을 특이적으로 억제하는 guanidine 유도체중에서 그 억제효과가 큰 guanidine hydrochloride(GH)를 도입하여 전염성 연화병 바이러스의 증식억제효과에 관하여 조사하였다. 1. 누에에 대한 GH의 약해시험 결과에서는 GH농도 0.01% 수준 이하에서는 약해가 전혀 없었다. 2. FV 감염잠 혼입에 따른 피해조사에서는 화용비율은 무처리(대조) 89.2% (100)에 대하여 1% 감염잠혼입구 70.7%(79), 5% 감염잠 혼입구 38.4%(43)이었으며 1만두수견양은 대조구 21.3kg(100)에 대하여 1% 혼입구 16.7kg(77), 5% 혼입구 8.8kg(41)으로서 전자와 같은 경향으로 나타났다. 3. FV에 대한 GH의 발병억제효과는 GH 0.01% 농도에서 대조구에 비하여 약 10배의 억제효과가 있었다. 4. GH의 투여회수와 발병억제효과에서는 FV접종후 계속 투여하는 것이 가장 효과적이었다. 5. FV 접종후 GH투여시간에 대한 발병억제 효과는 접종과 동시에 투여는 것이 가장 효과적이었다. 6. FV의 GH에 의한 시험관내 불활화조사에서는 GH가 시험관내에서 FV를 불활화시키는 작용이 인정되지 않았다.

• 한국식생활문화학회지
• /
• 제20권4호
• /
• pp.416-420
• /
• 2005

This study was carried out to make functional laver added with sericulture powder which have a effect of prevention adult diseases such as diabetes mellitus, hypertention, and obesity. In our lab. a lot of study have been performed about functional effect of sericulture(mulberry leaf, silkpeptide and silkworm) for examples decrease of serum cholesterol, triacylglyceride and control of serum glucose in rat. sericulture powder was prepared by pulverizing freeze-dried. Functional laver samples were prepared, and the sensory evaluation quality, physical characteristics and mineral contents of those were compared. In case of overal quality of sensory evaluation, silkpeptide powder laver was lower than other samples. The content of moisture of functional laver added with sericulture powder samples(control, sample 1, 2, 3) were 0.30, 0.98, 0.24 and 0.28%, respectively. The content of crude protein of functional laver added with sericulture powder samples were 40.50, 44.10, 56.75, and 62.50%, respectively. The content of crude fat of functional laver added with sericulture powder samples were 4.00, 4.10, 4.00 and 4.40%, respectively. The content of ash of functional Laver added with sericulture powder samples were 7.07, 7.53, 7.60 and 7.27%, respectively. The content of calcium of functional laver added with sericulture powder samples were 219, 253, 224 and 229mg/100g, respectively. The content of potassium of functional laver added with sericulture powder samples were 1879, 1919, 1890 and 1907mg/l00g, respectively. The content of iron of functional laver added with sericulture powder samples were 18.03, 18.23, 18.46 and 18.53mg/100g, respectively. The content of zinc of functional laver added with sericulture powder samples were 1.86, 4.47, 4.57 and 5.64mg/100g, respectively. The content of manganese of functional laver added with sericulture powder samples were 4.50, 8.50, 7.00 and 6.00 mg/100g, respectively. It was concluded that this functional laver added with sericulture powder is low calorie, high protein and high mineral health food which have a preventive effect of adult disease.

• 대한간호학회지
• /
• 제31권1호
• /
• pp.7-19
• /
• 2001

In our country, patients with diabetes mellitus are searching for complementary treatments to recover from illness, while they received treatments from the doctor. However, have been evaluated or investigated systematically. This study was carried out to explore application of complementary treatments for patients with DM. For this survey, questionnaires were developed by researchers and the data was collected from July to October of 1999. Among the 223 subjects, there is one general hospital, one oriental hospital, 15 area C.H.P, ahd 2 area health centers. The results were as follows: 1. The total number of cases was 223 and the average age was 62.85 years old and average duration of DM was 8.1 years. The number of patients who had experience with alternative therapies was 145 (65%). The number of those who have not undergone treatments was 78 (35%). 2. The 43.5% of experienced CAM were advised family and relatives. Only 30.3% approved the effect of CAM and 52.5% said that If another a new CAM is introduced, they will try it. 4. Ninety three kinds of complementary treatments were used. Among the used items, 63.7% was various types of plants, 21.6% was animal material and 14.7% was the mixed group. As a single item, Bombyx Mori (Silkworm) was the most frequently used (10.5%) followed by the bean, mushroom, Morus bombycis (mulberry), Ginseng, Commelina Communis (Dalgaebi), Chinese medicine, root of Rosa rugosa (Haedangwha). 5. Among the used items, Trichosanthes kirilowii Max. Eucommia ulmoides Oliver, Commelina Communis, Aralia elata, pine needle, mulberry fruit, root of Rosa rugosa. Ginseng, Lycii Fructus, Dioscorea radix, Polygonatum odoratum, Cassia tora L, Bombyx Mori, loach, Crucian carp were based upon the pharmacological function of effect for control of diabetes mellitus symptom. 6. In the analysis of the relationships between the general characters of the patients with new complementary alternative medicine try and hospital treatment; 1) The shorter group suffered from DM (p=.038), poor Self-MBG (p=.037) and wanted to try new complementary alternative medicine. 2) The group of DM education experience were carried out hospital treatment well (p=.045). In conclusion, further study will be required for the patients experience using alternative therapies as the D-M in terms of holistic view of patients.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제30권1호
• /
• pp.6-16
• /
• 2015

This study established the genetic characterisation of 10 microsporidian isolates infecting non-mulberry silkworms (Antheraea assamensis and Samia cynthia ricini) collected from biogeographical forest locations in the State of Assam, India, using PCR-based markers assays: inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD). A Nosema type species (NIK-1s_mys) was used as control for comparison. The shape of mature microsporidian spores were observed oval to elongated, measuring 3.80 to $4.90{\mu}m$ in length and 2.60 to $3.05{\mu}m$ in width. Fourteen ISSR primers generated reproducible profiles and yielded 178 fragments, of which 175 were polymorphic (98%), while 16 RAPD primers generated reproducible profiles with 198 amplified fragments displaying 95% of polymorphism. Estimation of genetic distance coefficients based on dice coefficients method and clustering with un-weighted pair group method using arithmetic average (UPGMA) analysis was done to unravel the genetic diversity of microsporidians infecting Indian muga and eri silkworm. The similarity coefficients varied from 0.385 to 0.941 in ISSR and 0.083 to 0.938 in RAPD data. UPGMA analysis generated dendrograms with two microsporidian groups, which appear to be different from each other. Based on Euclidean distance matrix method, 2-dimensional distribution also revealed considerable variability among different identified microsporidians. Clustering of these microsporidian isolates was in accordance with their host and biogeographic origin. Both techniques represent a useful and efficient tool for taxonomical grouping as well as for phylogenetic classification of different microsporidians in general and genotyping of these pathogens in particular.

• 한국잠사곤충학회지
• /
• 제17권2호
• /
• pp.97-100
• /
• 1975

추잠기의 적엽정도가 지조의 수량에 미치는 영향을 알기 위하여 시험을 한 바 다음과 같은 결과를 얻었다. (1) 추기에 있어서의 적엽의 영향은 조장이나 조률과 같은 형태적인 면보다는 조량과 같은 질적인 면에 더 크게 나타나고 있다. 2. 조량은 중간부적엽구를 제외하고는 추잠기의 적엽량과 반비례하여 무적엽구, 불반부적엽구, 중간부적엽구가 크고 상반부적엽구와 전적엽구는 작았는데 중간부적엽구는 추기의 적엽량이 많았는데도 조량이 컸다. (3) 춘잠기의 수량은 조량과 정비례의 관계에 있어 조량이 큰 무적엽구, 중간부적엽구, 하반부적엽구의 순위대로 많았고, 상반부적엽구, 전적엽구의 순으로 적었다. (4) 연간합계수량은 중간부적엽구가 가장 많아서 무적엽구에 비한다면 36％, 가행법인 전적엽구(상단 5잎 남기고)보다는 13％정도 많았다.

• 복식문화연구
• /
• 제21권6호
• /
• pp.844-859
• /
• 2013

This study investigated the characteristics and changes of cotton and silk textile production in Honam district, especially in Gurye, Jella Province from the 1930s to the 1980s. To do this, research method in this study was both literature and fieldwork research and results were as follows. First, in terms of cotton fiber cultivation of Gurye, Chinese Cotton(在來綿) has been substituted for America Cotton(陸地綿) and additionally, Yellow Cotton (黃綿) has been cultivated in the 1930s. Also, in terms of silk fiber cultivation of Gurye, Joseon Silkworm has been substituted for Japanese Silkworm by inflowing the Japanese mulberry tree. Second, in terms of spinning method, cotton spinning has been conducted at every house by an individual tool from the 1930s to the 1950s and has been gradually changed to mechanization by market shop equipped with mechanized cotton gin and cotton whipping tool. However, there have been no changes in silk spinning method from the 1930s to the 1980s. Third, loom type has been changed from the traditional Korean back-strap loom to the treadle loom between the 1930s and the 1940s. Fourth, dyeing was conducted by chemical dyestuff after weaving. The circulation of textile was done through the joint market by Japan in the 1930s~1950s and has changed to the market sales by producers since the 1950s. Fifth, since the 1970s, the outputs of cotton and silk textile of Gurye have been reduced. This was connected with the westernization and the inflow of the synthetic fiber and cloth in cotton and was related to the changes of the nation policy and silk fiber inflow from the china to the Korean farm villages.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제17권1호
• /
• pp.115-120
• /
• 2008

Recently, there has been the gradual growth of the public in rural amenities, hobby farming works, much more urbanities, and especially near-retires. However, in spite of popular preference to ruralities, little has been known about their demand on retired rural life. Therefore, this study examined urbanites' attitude to preparatory works for out-migration, preferred residential site condition and life styles in the countryside. For the purpose, data were collected from the survey with the sample of 62 urban trainees who were studying farming technology in department of training on agricultural technology of Korea National Agricultural College, RDA. We carried out the experience farming to candidate with using the abandoned sericultural facilities which are the silkworm rearing house and mulberry fields. The 71% of urban trainees, who answered in this survey, hoped to move into countryside after retirement and to make a monthly income of $1,000 from agriculture. The age distribution of sericulural farmers is about 79% more than that of 60 years old. In the experience farming works. The two candidates made 1,977$ with net income through 32 days worked in spring season. In autumn season, the couple candidates made 1,317$ with net income through 23 days worked. Knowledge of these results will be important to policy-makers and to understand the role that retirement in-migration play in stimulating economic growth of rural agricultural farmers.

• 한국잠사곤충학회지
• /
• 제35권2호
• /
• pp.86-88
• /
• 1993

요소를 뽕잎에 살포한 후 경과일 수가 누에 생육 및 견질에 영향을 주는가를 알기 위해 0.5% 요소수용액을 6회 살포하고 살포 1, 2, 3일 후에 수확하여 뽕잎의 화학성분을 분석하고 늦가을누에를 사육한 결과 다음과 같은 결과를 얻었다. 1. 요소 엽면시비를 대조에 비해 뽕잎의 수분함량을 0.4%~0.7%, 전 질소함량을 0.19~0.51% 더 높였으나, 다른 무기성분함량에는 영향이 없었다. 2. 뽕잎중의 NO3-N 함량은 요소엽면시비구가 대조에 비해 503~1,107 ppm 높았으며, 살포 후 시일이 경과함에 따라 함량이 감소하였다. 3. 누에 사육결과 대조구보다 시비구에서 전견종은 0.02~0.14g, 견층중은 0.5~3.1cg 높았으나 통계적인 유의차는 인정되지 않았다. 4. 요소엽면시비에 의해 뽕잎중의 NO3-N 함량은 증가하였지만, 생물 검정 결과 살포후 1일 경과한 뽕잎을 따먹여도 누에에는 전혀 해가 없는 것으로 판단되었다.

• Applied Biological Chemistry
• /
• 제41권2호
• /
• pp.187-190
• /
• 1998

동결건조(凍結乾燥)한 누에 유래(4령유충(齡幼蟲), 암 수 번데기, 암 수 성충(成蟲)) 및 건조 뽕나무 유래 재료(잎, 오디, 상백피(桑白皮))의 메탄을 추출물, 백강잠(白彊蠶) 및 누에 4령유충(齡幼蟲) 잠분(蠶糞)의 메탄올 추출물의 5종 인간(人間) 암세포주(癌細胞株)(A549 lung, SK-OV-2 ovarian, SK-MEL-2 melanoma, XF-498 CNS, HCT-15 colon tumor cell lines)에 대한 세포독성(細胞毒性)을 sulforhodamine B법을 이용하여 in vitro 검정하였다. 공시시료중 잠분(蠶糞)의 70% 메탄올 열탕추출물(熱湯抽出物)은 이들 암세포주(癌細胞株)에 대하여 강한 세포독성(細胞毒性)을 나타내었으나, 잠분(蠶糞)의 메탄올 추출물 및 오디와 상백피(桑白皮)의 메탄을 추출물은 중간 정도의 활성을 보였다. 기타 물질들은 이들 암세포주(癌細胞株)에 거의 독성을 보이지 않았다. 70% 메탄올 열탕추출물(熱湯抽出物)이 강한 세포독성(細胞毒性)을 나타내어, 용매 분획한 결과 클로로포름과 에틸아세테이트 획분이 암세포주(癌細胞株)에 대하여 가장 강한 세포독성(細胞毒性)을 보였다. 결론적으로, 잠분(蠶糞), 상백피(桑白皮) 및 오디의 항암활성(抗癌活性)은 이들의 약리작용의 일부를 설명할 수 있을 것으로 생각된다.