• Korean Journal of Environmental Agriculture
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• v.36 no.4
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• pp.249-255
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• 2017

BACKGROUND: Globally, mulberry (Morus sp.) is exploited for feeding leaf to silkworms in order to obtain silk fiber or for animal feedstock production. Also, mulberry fruit is known to a by-product that was produced from mulberry tree after harvesting leaves for silkworm rearing, as a yield and consumption of mulberry fruit was increased, it has been fixing to a newincome crop. Mulberry leaves and fruits are used for the health benefits of human beings. Mulberry contains various bioactive components, such as alkaloids and flavonoids. Mulberry flavonoids are an important part of the diet because of their effects on human nutrition. The flavonoids in mulberry leaf and fruit of 'Suhyang'(Morus alba L.) were determined. METHODS AND RESULTS: Flavonoids for mulberry leaf and fruit of 'Suhyang' were analysed using ultrahigh performance liquid chromatography coupled with diode array detection and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF/MS)technique. An UPLC-DAD-QTOF/MS system was used, and identification of mulberry leaves constituents was carried out on the basis of the complementary information obtained from LC spectra, MS ions, and MS/MS fragments. The mulberry leaf (16 flavonoids) and fruit (9 flavonoids) were isolated and analyzed from Suhyang using UPLC-DAD-QTOF/MS chromatogram. To the best of our knowledge, Quercetin 3-O-(6"-O-malonyl) glucoside and quercetin 3-O-rutinoside (rutin) was detected on the highest content in leaf and fruit, respectively and further research will be devoted to evaluate their biological activity. CONCLUSION: Obtaining information about the concentration of functional materials in mulberry leaves could contribute to the development and promotion of processed, functional products and offer possible industrial use of 'Suhyang', holding promises to enhance the overall profitability of sericulture.

• Journal of Sericultural and Entomological Science
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• v.29 no.1
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• pp.12-19
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• 1987

This study was carried out to investigate the effects of climatic conditions on the budding of buds and the growth of shoots of mulberry (Morus alba L.) at Suwon for the period of six years from 1978 to 1983. The results are summarized as follows. 1. The period that influenced most greatly on mulberry budding and foliating of it's buds was from late part of March to middle part of April in the district of Suwon, Korea. 2. Temperatures in soil 20cm deep during the period from late part of March to middle part of April had high correlations with budding dates. 3. It was disclosed that the budding date had high correlations with the date of bud shaped swallow-bill (r=0.9861**), date of the third leaf(r=0.97**), and date of the fifth leaf(r=0.96**), respectively. 4. The higher the average temperature of April became, the longer the length of shoots became. 5. The earlier the budding date and foliating date came, the larger the leaf yield became. However, with excessive amount of precipitation after early budding and foliating, the leaf yield was not increased in proportional. 6. The longer the growing period of the shoots became, the larger the leaf yield became.

• International Journal of Industrial Entomology and Biomaterials
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• v.2 no.1
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• pp.75-78
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• 2001

The whitefly, Dialeuropora decempuncta Quaintance and Baker (Homoptera : Aleyrodidae) causing considerable damage to mulberry, Morus alba. Crop loss caused by the whitefly was estimated in mulberry. Quantitative yield loss was estimated on the basis of harvest data from both sprayed and unsprayed plots. Data on pest incidence has been recorded at weekly intervals in both sprayed and unsprayed plots from 30 th day of plant age till harvesting i.e., 60 days after pruning in August October season. An attempt has been made to establish a relationship between whitefly population and percentage of crop loss due to it. Percentage of crop loss due to whitefly has got a linear relationship with the whitefly population. An initial population of 24 adults/top leaf would be able to cause 24% loss (l,630 Kg leaf/ha ) in a period of 30 days. Economic analysis postulates that application of pesticide can stove a net amount of 1,630 Kg leaf/ha which is sufficient to produce 67.65 Kg of additional multivoltine cocoons. This determines the cost/benefit ratio which will enable to workout the economics of management practices.

• International Journal of Industrial Entomology and Biomaterials
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• v.19 no.1
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• pp.165-169
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• 2009

A field experiment to evaluate the effect of application of different organic manures and inorganic fertilizers on growth, yield and quality of leaf was studied during 2004-05 has showed that, the application of 10 kg each of Azospirillum brasilense and Aspergillus awamori+20% each of recommended N through compost+green manure (Glyricidia maculata)+castor cake+vermicompost+Urea and remaining P and K through fertilizers ($T_{11}$) has recorded significantly higher leaf yield (250 g/ plant and 34.70 tonnes/ha/yr, respectively) with improvement in growth characters as compared to control. Leaf quality status was also improved in terms of N (3.19%), P (1.97%), K (1.28%), total soluble protein (8.39 mg/ml), total soluble sugars (14.40 mg/ml), secondary nutrients viz., Ca (3.00%), Mg (0.60%), S (0.35%) and micronutrients viz., Cu (0.410 ppm), Mn (0.454 ppm) and Zn (0.112 ppm) contents. The mulberry grown with 20 tonnes of compost+300:120:120 Kg of NPK/ha/year through fertilizer ranked second for growth and foliar constituents.

• The Korean Journal of Community Living Science
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• v.14 no.2
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• pp.63-70
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• 2003

In this experiment, the curd was made from soybean and cow's milk in the ratio of 7 to 3, and coagulated with Glucono-delta-lacton(GDL) and $CaSO_4&.$2H_{2}O& in the 1:1 ratio. During the procedure, the mulberry leaf powder was added. The amount of the added powder varied from 0.1 % to 0.5 %. This experiment aimed to show the change of yield, general components, minerals, amino acid, changes of pH, sensory properties of these products. As mulberry leaf powder addition increased, crude protein, crude fat, crude ash content, minerals and amino acids increased. Whereas moisture content decreased, and in particular,$Ca^{++}$, $K^+$ and $Na^+$ increased. On the other hand, in the sensory properties, the levels of satisfaction about the color, appearance, flavor, texture and savory were lower in mulberry leaf powder added than under control.

• Journal of Sericultural and Entomological Science
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• v.36 no.2
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• pp.102-109
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• 1994

Profitability was analysed in the fields of various planting densities from 1,666 trees/10a to 4,166 with mulberry grafts or conventional saplings under the consideration of leaf yield and quality. Leaf yield per tree decreased with the higher planting densities. Seasonal and total leaf yield per area, however, increased by 20 to 63% as a mean for 3 years in the densities of 2,083~4,166 trees per 10a than in the conventional density of 1,666 trees per 10a. The increase in leaf yield per area was not so high in the densities over 2,083. Leaf yield in the sapling plots was a little higher than that in the graft plots. Topping of shoot tip affected neither on the branch length nor on yield. Yield was higher in the planting spacing with single raws than in that with double raws. Quality of leaves in the densities from 2,083 to 3,333 trees per 10a was relatively good judged based on the results of the pupation rate and cocoon yield and quality.

• Journal of Sericultural and Entomological Science
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• v.35 no.1
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• pp.11-16
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• 1993

Field experiment and silkworm rearing were conducted for 4years in an attempt to reduce phosphorus fertilizer in mulberry field with high levels of phosphate. Experiments consisted of four treatments; normal recommended P application(13kg P2O5/10a), half, null and no fertilizer on each of 3 mulberry fields, two fields with 300ppm and one filed begining with 450ppm of aviable P2O5 content in soil. One of 300ppm fields was used for leaf quantity and one for leaf quality test by silkworm rearing. Leaf yield in the 300ppm field decreased after the 3rd spring in the no phosphorus treatment and in the 4th fall in the half phosphorus treatment. No yield decrease occurred in 450ppm field for 4 years. Yield decrease did occur in the next rearing season after soil phosphate content in the 10~20cm zone dropped below 150ppm. Phosphorus fertilizer should be applied at this time. Though P2O5 concentration in leaves of no phosphorus treatment was 0.18% lower that that of conventional treatment, there were no difference in cocoon quality.

• International Journal of Industrial Entomology and Biomaterials
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• v.18 no.2
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• pp.97-103
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• 2009

Sericulturists in the vicinity of Bangalore city irrigate their mulberry gardens with Vrishabhavathy stream water, which is polluted with domestic and industrial wastes from the city. To investigate the effect of pollutants on silkworm crops, a detailed field survey was conducted to study the cocoon yield pattern of the crops raised on mulberry irrigated with wastewater as against irrigation by bore/open wells. The villages along the stream were grouped into five test batches at about a distance of $5{\sim}8$ km from each other. The seasonal yield data with relevant information were collected through questionnaires from 117 rearers using stream water and 35 rearers using bore/open wells, the latter serving as control group. The average yield for 100 layings was 46 to 57 kg in the control group whereas in test groups, it ranged between 34 to 51 kg in the first test group and 22 to 38 kg in the rest. The difference in yield was $9{\sim}19$ kg depending on the season between control and test batches. In summer, this difference was higher, with high co-efficient of variation in test groups ($33{\sim}52$%). Further, water, soil and leaf samples were collected from selected rearers and were analyzed for zinc, copper, iron, lead and nickel. Results indicated significantly higher contents of these metals in samples from gardens using wastewater when compared control samples. Significantly (p<0.05 & p<0.01)) higher levels of zinc ($24{\sim}122$ ppm) and iron ($208{\sim}683$ ppm) were noticed in mulberry leaves during summer followed by winter and rainy season. The significance of high content of heavy metals in mulberry leaves and cocoon yield pattern of this area in relation to the quality of irrigation water is discussed.

• Journal of Sericultural and Entomological Science
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• v.10
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• pp.27-33
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• 1969

This study was carried out to investigate the variation of mulberry yield in various parts of mulberry field. The results obtained from the study are summarized as follows. 1. For all the varieties, higher yield was observed side part than in the middle part. 2. With respect to the direction of field, the side faced south produced the highest yield, which was followed both side parts faced east and west. The side part fated north also produced relatively high yield. 3. No significant difference in yield was obtained between the central part and the near the each side, even the former seemed to be some what higher in yield than the latter. 4. Such a variety as Kaeryangsuban havins small leaves on many twigs with small internodes appeared to have more side effect than the otherwise variety, i.e. Rosang. 5. The variety Kaeryangsuban, showed more side effect in spring than in Autumm, however Rosang showed reversed effect. 6. Similar results were obtained in the leaf dry weight. 7. As a conclusion, it can he an important factor to increase yield to set up the mulberry field in east and west resulting less side effect.

• Journal of Sericultural and Entomological Science
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• v.53 no.2
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• pp.71-77
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• 2015

Much attention has been focused on the activity of the natural antioxidants present in fruits and vegetables, because potentially these components may reduce the level of oxidative stress. Especially, mulberry leaves containing many natural components are considerable resource for natural antioxidants. The antioxidant capacity of mulberry leaves was investigated with minilum L-100 device and ARAW-KIT (anti-radical ability of water-soluble substance), in comparison to the ascorbic acid. The antioxidant capacity of 16 varieties was 3303.4 nmol at opening stage of five leaves in spring. The highest stage of antioxidant capacity (3708.0 nmol) and yield rate was just before the coloration stage with anthocyanin in fruits, whereas the lowest stage was middle of June (2231.6 nmol) and about two months growing stage after summer pruning (2064.6 nmol). But after summer pruning, the antioxidant capacity of mulberry leaves increased gradually until just before fallen leaves stage. Even if samples were same variety, antioxidant effect of those showed different results according to collected regions. Also, antioxidant effect of mulberry leaves were higher than that of branches. The antioxidant capacity of yield-type mulberry leaves and fruits (Morus alba L., M. bombycis Koidz, and M. Lhou (Ser.) Koidz) collected from In-je, Won-ju and Yang-yang regions, Kang-won province, Korea, was investigated. The results indicated that total antioxidant capacity of yield-type mulberry leaves was 2711.2 nmol. In the antioxidant capacity analysis of Jeollabuk-Do genetic resources, autumn's mulberry leaves showed higher antioxidant capacity than that of spring's it. To investigate the effect of tea on antioxidative capacity, five kinds of tea(coffee mix, green tea added brown rice, mulberry leaf tea, Polygonatum odoratum tea and black tea added lemon) were selected and analyzed. Their's anti-oxidative capacity were 2,531.01 nmol, 1,867.42 nmol, 1,053.72 nmol, 292.71 nmol and 188.91 nmol, respectively. The antioxidative capacity of drinking water soaked with mulberry leaf showed 891.96 nmol.