• Journal of The Geomorphological Association of Korea
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• v.19 no.2
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• pp.51-68
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• 2012

This paper aims to investigate the value in culture, history and ecology of the Mt. Palgong. It attempts to build the strategy for making use of The Mt. Palgong by literature survey, interview, and field survey. The main results are as follows: 1) The boundary of physical geography of the Mt. Palgong is classified into two. The boundary of the Mt. Palgong limits to granite mass block in a narrow sense, while in a broad sense, the boundary of the Mt. Palgong includes a contact aureole to be bordered on the Mt. Palgong granite mass block. The boundary from a cultural viewpoint limits to Daegu city(excluding Dalseong county) and Gyeongsan city, Yeongcheon city, Gunwi county and Chilgok (Dongmyeong-myeon, Giseong-myeon) in Gyeongbuk province. 2) In the geological boundary, one of the south-west slope is clearer than that of the north-east slope of the Mt. Palgong. The landforms such as tor, sheeting joint and gutter are well developed as a whole. Mountain landform such as boulder stream, polygonal cracking is relatively well developed on the south-west slope, while river landform is relatively well developed on the north-east slope of the Mt. Palgong. 3) It is necessary to develope various masterpiece of interesting stories related to Mt. Palgong in order to make the Mt. Palgong excellent tour complex. 4) It is desirable to designate the Mt. Palgong as a national park for systematic management. A master plan should be ultimately designed to raise brand value of Daegu city, and make good identity of the city by restoring 'The Mt. Palgong Jecheondan' and registering 'Gatbawi' as world heritage. 5) It is reasonable that the method of development in The Mt. Palgong should be based on the pattern of 'slow life town'. 'The Mt. Palgong museum' will then be designed to give visitors all the informations on The Mt. Palgong.

• Korean Journal of Plant Resources
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• v.7 no.2
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• pp.121-126
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• 1994

Many Entoloma were collected at Mt. Baiyang, Mt. Jiry, Mt, Sunun, Mt, Naejang and Mt. Manduck to September 1991 from June 1991. These spercies were identified. According to the resulting. Entoloma readii, E. cinerascens, E.padiceum, E.squamulosum and E. umbilicatum were newly to Korea.

• Journal of the Korean Geographical Society
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• v.51 no.1
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• pp.127-142
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• 2016

This paper analyzes on the physical place name of Mt. Seoraksan in Joseon period from the historical geographical perspective. The analysis materials are Jirijis, old maps and travel records of Joseon period. The results are as follows. The space range of Mt. Seoraksan in Joseon period and today was different. Mt. Seoraksan in Joseon period was around Daecheongbong Peak nowadays, Ulsanbawi Rock was Mt. Cheonhusan, and north mountain range of Hangyeryeong Pass called Mt. Hangyesan. Region of Mt. Seoraksan and Mt. Hangyesan were devided in Joseon Period. Mt. Seoraksan name was representative in late Joseon period including Mt. Hangyesan and Mt. Cheonhusan. Mountain names were the most in the physical place name. The old name of Hangyeryeong Pass was Osaek Pass in maps of Joseon Period. The popular travel course of Mt. Seoraksan in Joseon period was Baekdamsa Temple-Oseam Temple-Sinheungsa Temple.

• Journal of Microbiology and Biotechnology
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• v.17 no.5
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• pp.728-732
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• 2007

In the present article, a novel fusion expression vector for Escherichia coli was developed based on the pTORG plasmid, a derivative of pET32a. This vector, named pT7MT(GenBank Accession No DQ504436), carries a T7 promoter and it drives the downstream gene encoding Metallothionein 2A(MT2A). There are in-framed multiple cloning sites(MCS) downstream of the MT2A gene. A target gene can be cloned into the MCS and fused to the C-terminal of the MT2A gene in a compatible open reading frame(ORF) to achieve fusion expression. The metal-binding capability of MT2A allows the purification of fusion proteins by metal chelating affinity chromatography, known as $Ni^{2+}$-affinity chromatography. Using this expression vector, we successfully got the stable and high-yield expression of MT2A-GST and MT2A-Troponin I fusion proteins. These two proteins were easily purified from the supernatant of cell lysates by one-step $Ni^{2+}$-affinity chromatography. The final yields of MT2A-GST and MT2A-Troponin I were 30mg/l and 28mg/l in LB culture, respectively. Taken together, our data suggest that pT7MT can be applied as a useful expression vector for stable and high-yield production of fusion proteins.

• Archives of Pharmacal Research
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• v.20 no.6
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• pp.560-565
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• 1997

The physicochemical properties of melatonin (MT) in propylene glycol (PG) and 2-hydroxypropyl-.betha.-cyclodextrin $(2-HP{\beta}CD)$ vehicles were characterized. MT was endothermally decomposed as determined by differential scanning calorimetry (DSC). Melting point and heat of fusion obtained were $116.9{\pm}0.24^{\circ}C $.and $7249{\pm}217 cal/mol$., respectively. MT as received from a manufacture was very pure, at least 99.9%. The solubility of MT in PG solution increased slowly until reaching 40% PG and then steeply increased. Solubility of MT increased linearly as concentration of $2-HP{\beta}CD$ without PG INCREASED$(R^2=0.993)$. MT solubility in the mixtures of pg and $2-HP{\beta}CD$ also increased linearly but was less than the sum of its solubility in $2-HP{\beta}CD$ and PG individually. The MT solubility was low in water, simulated gastric or intestinal fluid but the highest in the mixture of PG(40v/v%) and $2-HP{\beta}CD$ (30w/v%) although efficiency of MT solubilization in $2-HP{\beta}CD$ decreased as the concentration of PG increased. MT was degraded in a fashion of the first order kinetics $(r^2>0.90)$. MT was unstable in strong acidic solution (HCl-NaCl buffer, pH 1.4) but relatively stable in other pH values of 4-10 at $70^{\circ}C$. In HCl-NaCl buffer, MT in 10% PG was more quickly degraded and then slowed dpwm at a higher concentration. However, the degradation rate constant of MT in 2-HP.betha.CD was not changed significantly when compared to the water. The current studies can be applied to the dosage formulations for the purpose of enhancing percutaneous absorption or bioavailability of MT.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.2
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• pp.19-39
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• 2016

This study was carried out to investigated the flora of Naknam-Jeongmaek, Korea. The survey sites were Gilmajae, Mt. Muryang, Mt. Yeohang, Mt. Muhak, Mt. Cheonju and Mt. Sineo. The flora were summarized, numbering 666 taxa including 112 families, 357 genera, 574 species, 4 subspecies, 73 varieties and 15 forms. The threatened species was Aconitum austrokoreense. The rare plants were 12 taxa including Asarum maculatum, Wikstroemia ganpi, Viola albida, Tricyrtis macropoda and so forth. The Korean endemic plants were 19 taxa including Salix koriyanagi, Thalictrum actaefolium var. brevistylum, Weigela subsessilis, Hosta minor and so forth. The specific plants by floristic region were 81 taxa including 38 taxa of grade I, 19 taxa of grade II, 16 taxa of grade III, 7 taxa of grade IV and 1 taxa of grade V. The naturalized plants were 32 taxa including Rumex crispus, Physalis angulata, Erechtites hieracifolia, Taraxacum officinale and so forth. The invasive alien plants were Ambrosia artemisiifolia and Aster pilosus.

• Journal of Oriental Neuropsychiatry
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• v.21 no.2
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• pp.103-123
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• 2010

Objectives : This study was to investigate the effect of several herbal prescriptions such as Tianwangbuxindan qu zhusha, Tianwangbuxindan, Wendantang, Guipitang on the level of $MT_1$ and $MT_2$ melatonin receptors in C6 glial cells. Methods : For this study, we exposed of C6 cells to several herbal prescriptions resulted in non-cytotoxic in various dose as measured by MTT assay, trypan blue count, morphology change and DAPI stain. Results : Tianwangbuxindan qu zhush and Tianwangbuxindan induced the levels of $MT_1$ melatonin receptor expression in a dose-dependent manner without altering the level of $MT_2$ melatonin receptor expression. However, the treatment with Wendantang, Guipitang don't effect of $MT_1$ and $MT_2$ melatonin receptor expression. Conclusions : This results suggest that Tianwangbuxindan can be a promising the regulation of melatonin receptor synthesis, and further studies will be needed to clarify the mechanism.

• Animal cells and systems
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• v.5 no.3
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• pp.263-266
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• 2001

Metallothionein (MT) is induced in the regenerating rat liver. We have investigated expression of MT gene by RT PCR as well as specific localization of MT by immunocytochemistry in regenerating rat liver after partial hepatectomy (PH). MT mRNA level started to increase from 1 h and reached the peak at 8 h after PH. The level decreased gradually by 24 h, and became similar to that of control group. In the immunocytochemical study, in all groups treated with primary antibody, immunogold particles indicating the presence of MT were evenly distributed throughout both cytoplasm and nucleus of the rat hepatocytes. Within the nucleus, the gold particles appeared to be intensely localized in the areas of euchromatin and nucleolus. Within the cytoplasm, gold particles did not seem to adhere to mitochondria or Iysosomes, but were freely distributed. However, rough endoplasmic reticulum was the obvious compartment on which the gold particles were localized. Time course of MT immunoreactivity revealed that distribution of gold particles in hepatocytes increased gradually by 24 h, and decreased at 48 h after PH. Briefly, PH resulted in the sharpest increase in the expression of MT mRNA at 8 h and in the immunoreactivity of MT at 24 h, respectively. It is suggested that the increase of MT mRNA expression, the intensity of immunoreactivity and the specific localization of MT may be associated with the compensatory cell proliferation followed by PH.

• Journal of Life Science
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• v.27 no.1
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• pp.100-121
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• 2017

The sexual reproduction of the unicellular green alga Chlamydomonas is reviewed for a comprehensive understanding of the complex processes. The sexual life cycle of C. reinhardtii is distinguished into five main stages: gametogenesis, gamete activation, cell fusion, zygote maturation, and meiosis and germination. Gametogenesis is induced by nitrogen starvation in the environment. C. reinhardtii has two mating types: mating type plus ($mt^+$) and mating type minus ($mt^-$), controlled by a single complex mating type locus ($MT^+$ or $MT^-$) on linkage group VI. In the early gametogenesis agglutinins are synthesized. The $mt^+$ and $mt^-$ agglutinins are encoded by the autosomal genes SAG1 (Sexual AGglutination1) and SAD1 (Sexual ADhesion1), respectively. The agglutinins are responsible for the flagellar adhesion of the two mating type of gametes. The flagellar adhesion initiates a cAMP mediated signal transduction pathways and activates the flagellar tips. In response to the cAMP signal, mating structures between two flagella are activated. The $mt^+$ and $mt^-$ gamete-specific fusion proteins, Fus1 and Hap2/Gcs1, are present on the plasma membrane of the two mating structures. Contact of the two mating structures leads to develop a fertilization tubule forming a cytoplasmic bridge between the two gametes. Upon fusion of nuclei and chloroplasts of $mt^+$ and $mt^-$ cells, the zygotes become zygospores. It is notable that the young zygote shows uniparental inheritance of chloroplast DNA from the $mt^+$ parent and mitochondrial DNA from the $mt^-$ parent. Under the favorable conditions, the zygospores divide meiotically and germinate and then new haploid progenies, vegetative cells, are released.

• The Korean Journal of Ecology
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• v.17 no.4
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• pp.443-452
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• 1994

A phytosociological study of the forest vegetation was carried out in Mt. Chongok and Mt. Tuta from June, 1992 to August, 1993. The forest vegetation was classified into 1 order, 2 alliances, 1 suballiance and 4 communities; Vaccinium hirtum v. koreanum-Quercus monogolica community, Carex humilis v. nana-Pinus densiflora community, Sasa borealis-Carapinus laxiflora community, Plectranthus inflexus-Quercus variabilis community. Actual vegetation map was drawn in 1:25,000 scale.