• BMB Reports
• /
• 제43권1호
• /
• pp.29-33
• /
• 2010

It is well known that PR/SET family members participate in transcriptional regulation via chromatin remodeling. PRDM10 might play an essential role in gene expression, but no such evidence has been observed so far. To assess PRDM10 expression at various stages of mouse development, we performed immunohistochemistry using available PRDM10 antibody. Embryos were obtained from three distinct developmental stages. At E8.5, PRDM10 expression was concentrated in the mesodermal and neural crest populations. As embryogenesis proceeded further to E13.5, PRMD10 expression was mainly in mesoderm-derived tissues such as somites and neural crest-derived populations such as the facial skeleton. This expression pattern was consistently maintained to the fetal growth period E16.5 and adult mouse, suggesting that PRDM10 may function in tissue differentiation. Our study revealed that PRDM10 might be a transcriptional regulator for normal tissue differentiation during mouse embryonic development.

• Korean Journal of Veterinary Research
• /
• 제44권2호
• /
• pp.159-162
• /
• 2004

We investigated the cytogenetic characteristics of male black-striped field mouse (Apodemus agrarium) in Korea. Chromosome slides were obtained from blood cell cultures which were synchronized with thymidine blocking or not. In the chromosome slide which synchronization with thymidine blocking was employed on, the GTG(G bands by trypsin using Giemsa)-bands of high resolution were observed. The male black-striped field mouse has 48 chromosomes composed 46 autosomes and XY sex chromosomes. The centromeric regions of autosomes were positive to GTG-banding. According to this investigation, thymidine blocking in cell culture process was useful to get lengthened chromosomes. It may be necessary to employ RBG-banding technique to investigate complementary band patterns between R- and G-banding in black-striped field mouse.

• Journal of Korean Society of Water and Wastewater
• /
• 제24권5호
• /
• pp.549-560
• /
• 2010

Recently, heavy rainfalls due to the climate change in Korea have caused inundation problems in urban sewer networks. In july 2006, a flooding accident at Misari motorboat racing stadium near the Han river occurred due to the effect of record-breaking outflow discharge from Paldang-dam. The purpose of this study was to simulate and analyze the flooding accident at Misari stadium by MOUSE model. The results of simulation analysis indicated that the total flood volume was $1,313,450m^3$. The effect of back water was 85.9% of the total volume which was caused by the manhole accident, and the effect of accumulated runoff was 14.1% of total volume which was caused by non-return valve shutdown. The simulation results of this MOUSE modeling that was linked to the boundary condition of the dynamic flows in the river by DWOPER model showed the potential of successful inundation analysis for sewer networks.

• Proceedings of the Korean Society of Toxicology Conference
• /
• 한국독성학회 2003년도 추계학술대회
• /
• pp.109-110
• /
• 2003

To validate a novel mouse model, gpt delta, for in vivo mutagenesis, the Mammalian Mutagenesis Society (MMS), a subgroup of the Environmental Mutagen Society of Japan (JEMS) (JEMS/MMS), performed a collaborative study as the third trial for transgenic animal assay. In this mouse model, point mutations and deletions re separately identified by gpt (6-thioguanine-resistant) and Spi- (sensitive to P2 interference) selections, respectively.(omitted)

• Journal of Microbiology
• /
• 제45권6호
• /
• pp.572-577
• /
• 2007

To enhance therapeutic potential of murine monoclonal antibody, humanization by CDR grafting is usually used to reduce immunogenic mouse residues. Most humanized antibodies still have mouse residues critical for antigen binding, but the mouse residues may evoke immune responses in humans. Previously, we constructed a new humanized version (AKA) of mouse CC49 antibody specific for tumor-associated glycoprotein, TAG-72. In this study, to select a completely human antibody light chain against TAG-72, guided selection strategy using phage display was used. The heavy chain variable region (VH) of AKA was used to guide the selection of a human TAG-72-specific light chain variable region (VL) from a human VL repertoire constructed from human PBL. Most of the selected VLs were identified to be originated from the members of the human germline VK1 family, whereas the VL of AKA is more homologous to the VK4 family. Competition binding assay of the selected Fabs with mouse CC49 suggested that the epitopes of the Fabs overlap with that of CC49. In addition, they showed better antigen-binding affinity compared to parental AKA. The selected human VLs may be used to guide the selection of human VHs to get completely human anti-TAG72 antibody.

• Journal of the Ergonomics Society of Korea
• /
• 제29권5호
• /
• pp.763-770
• /
• 2010

The use of web widgets on desktop and mobile devices has been increasing rapidly. Web widgets provide access to activities and information from various sources across the web. As the number of supported widgets increases, managing widgets and finding relevant or interesting widgets becomes more complex. In addition, interacting with widgets in web service systems can be difficult, especially for novice users. Up to this point, there has been little research on web widget usability. This paper performs an experimental study regarding user interfaces of web widgets based on the mouse tracking and work-flow analysis. In the experiment, four sites providing widget services are chosen - iGoogle, Netvibes, My yahoo, and Wizard. The experiment participants perform three assigned tasks in the chosen sites, and their mouse operations are recorded using Camtasia, a screen casting software. Mouse tracking analysis is performed based on the recorded data in order to analyze common user behaviors. In addition, work-flow diagrams representing the operational flows to carry out the given tasks in each web site are constructed so as to visually and systematically analyze detailed usage patterns. The experimental study results presented in this paper can contribute to developing guidelines for highly usable and accessible interface design of web widgets.

• Clinical and Experimental Reproductive Medicine
• /
• 제21권1호
• /
• pp.63-68
• /
• 1994

To confirm the overcome of in vitro 2-cell block, ICR mouse I-cell embryos were cultured in CZB media. All embryos in CZB were overcome in vitro 2-cell block and 92% of embryos were developed to the blastocyst at day 4. However, in m-KRB group(control) only 20% of embryos were developed over 2-cell. Any embryos in m-KRB did not develop to the morular stage. Developments and degenerations of ICR mouse I-cell embryos were compared in CZB medium prepared with water of three quality:(l) Milli-Q ultrafiltration water(UF);(2) Milli-Q reverse osmosis water(RO);(3) tap water(TAP). The objective was to evaluate the potential of quality control using ICR mouse 1-cell embryos. The more water was purified, the better embryo developments were supported and the less embryos were degenerated. As a quality control system, the culture of ICR 1-cell mouse embryos in CZB was useful.

• Journal of Korea Multimedia Society
• /
• 제15권11호
• /
• pp.1377-1383
• /
• 2012

This paper proposes a human mouse system that replaces mouse input by human hand movement. As the resolution of monitors increases, it is not quite possible, due to the resolution difference between web cameras and monitors, to place the cursor in the entire range of a monitor by simply moving the pointer which recognizes the position of the hand from the web camera. In this regard, we propose an effective method of placing the position of the mouse, without repeating the returning hand movements, in the corners of the monitor in which the user wants it to be. We also proposes the recognition method of finger movements in terms of using thumb and index finger. The measurement that we conducted shows the successful recognition rate of 97% that corroborates the effectiveness of our method.

• Biomedical Science Letters
• /
• 제14권3호
• /
• pp.167-172
• /
• 2008

We compared the clinical efficacy of LED therapy using 880 nm and 630 nm LED to test collagen accumulations in subcutaneous tissue of mouse after LED irradiation by measuring the quantity of collagen. 880 nm and 630 nm LED was irradiated on the back of ICR mouse given at $10.8J/cm^2$ followed for 30 minutes everyday for 5 weeks. Histological observation was performed by Hematoxylin & Eosin staining and Masson's Trichrome collagen staining. We also used Sircol soluble collagen assay kit for measuring the amounts of collagen in the mouse skin tissue after 1, 3, and 5 weeks post LED irradiation, respectively. Collagen generation was found at subcutaneous tissue, and the quantity of collagen in 880 nm LED group had grown more than that of 630 nm LED group at 5 weeks follow-up later. About 75% more efficacies for collagen generation were found in the group of 5th week of 880nm LED irradiation. The efficacy of 880nm LED could be more useful than 630 nm LED for synthesizing collagens in mouse subcutaneous tissue as time followed.

• Mass Spectrometry Letters
• /
• 제12권3호
• /
• pp.112-117
• /
• 2021

α-Amanitin and β-amanitin are highly toxic bicyclic octapeptides responsible for the poisoning of poisonous mushrooms such as Amanita, Galerina, and Lepiota by inhibiting RNA polymerase II, DNA transcription, and protein synthesis. A sensitive, simple, and selective liquid chromatography-high resolution mass spectrometric method using parallel reaction monitoring mode was developed and validated for the simultaneous determination of α- and β-amanitin in mouse plasma to evaluate the toxicokinetics of α- and β-amanitin in mice. Protein precipitation of 5 μL mouse plasma sample with methanol as sample clean-up procedure and use of negative electrospray ionization resulted in better sensitivity and less matrix effect. The calibration curves for α- and β-amanitin in mouse plasma were linear over the range of 0.5-500 ng/mL. The intra- and inter-day coefficient of variations and accuracies for α- and β-amanitin at four quality control concentrations were 3.1-14.6% and 92.5-115.0%, respectively. The present method was successfully applied to the toxicokinetic study of α- and β-amanitin after an oral administration of α- and β-amanitin at 1.5 mg/kg dose to male ICR mice.