• 한국멀티미디어학회논문지
• /
• 제19권1호
• /
• pp.60-67
• /
• 2016

PC users have long been controlling their computers using input devices such as mouse and keyboard. To improve inconveniences of these devices, the method of screen-touching has widely been used these days, and devices recognizing human gestures are being developed one after another. Fox example, Kinect, developed and distributed by Microsoft, is a non-contact input device that recognizes human gestures through motion-recognizing sensors, thus replacing the mouse as an input device. However, when controlling the mouse on a large screen, it suffers from the problem of requiring large motions in order to move the mouse pointer to the edges of the screen. In this paper, we propose a joystick-driven mouse-controlling method which enables the user to move the mouse pointer to the corners of the screen with small motions. The experimental results show that movements of the user's palm within the range of 30 cm ensure movements of the mouse pointer to the edges of the screen.

• Biomolecules & Therapeutics
• /
• 제13권1호
• /
• pp.7-12
• /
• 2005

GyeongshinhaeGihwan T1 (GGT1) is a newly developed oriental medicine to help weight control. We investigated nitric oxide production and cytokine secretion in mouse peritoneal macrophages. According to recent reports, macrophages are participated in fat accumulation and closely related with obesity. In this study, using mouse peritoneal macrophages, we have examined whether GGT1 affects the production of nitric oxide (NO), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), and interleukin (IL)-12 by the stimulation of interferon-${\gamma}$ and lipopolysaccharide (LPS). GGT1 inhibits LPS-induced NO production in a dose-dependent manner. The decrease in NO synthesis was reflected as a decreased amount of inducible NO synthese protein. We also found that GGT1 inhibits pro-inflammatory cytokines, TNF-${\alpha}$ and IL-12 production. In mouse embryo preadipocyte 3T3-L1, GGT1 reduced the viability in a dose-dependent manner. These findings suggest that GGT1 may have potential effects in preventing and controlling adipogenesis and obesity.

• 한국미생물·생명공학회지
• /
• 제32권1호
• /
• pp.11-15
• /
• 2004

본 연구에서는 C. diphtheriae toxin-A(DTA)를 합성하는 유전자를 tetracycline derivative인 doxycycline에 의해 발현이 유도되는 plasmid('Tet-on' system)에 삽입시켜, 이를 mouse ES cell에 도입시켰으며, 이렇게 제작된 mouse ES cell이 doxycycline의 처리 농도에 따라 mouse ES cell내의 DTA의 발현이 유도되어 이 결과 세포 사별(apoptosis)을 유발시키는 것을 MTT assay를 통해 확인하였다.

• 한국발생생물학회지:발생과생식
• /
• 제22권4호
• /
• pp.331-339
• /
• 2018

NUCB2/nesfatin-1 is first known to be expressed in the hypothalamus while controlling appetite and energy metabolism. However, recent studies have shown that NUCB2/nesfatin-1 was expressed in the various organs as well as the hypothalamus. Our previous reports also demonstrated that NUCB2/nesfatin-1 was expressed in the ovary, testis, pituitary gland, lung, kidney, and stomach of fetal and adult mice. However, the role of NUCB2/nesfatin-1 in mouse fetus remains unknown. Thus, the aim of this study was to investigate whether NUCB2/nestatin-1 is expressed in mouse fetus at the developmental stage in which organogenesis begins. To do this, we performed in situ hybridization (ISH) and immunohistochemistry (IHC) staining to examine the distribution of NUCB2 mRNA and nesfatin-1 protein in the mouse fetal organs during early developmental stages, especially at embryonic day (E) 10.5. As a result of ISH, NUCB2 mRNA positive signals were more frequent in the liver, but there were relatively few positive signals in heart. On the other hand, no positive signals were detected in other organs. These ISH results were validated by IHC staining and qRT-PCR analysis. Expression of nesfatin-1 protein detected by IHC staining was similar to that of NUCB2 mRNA detected by ISH in the liver and heart. In addition, the levels of NUCB2 mRNA expression analyzed by qRT-PCR were significantly increased in the liver and heart compared to other organs of the mouse fetus at E13.5, whereas its level was extensively decreased in the liver, but increased in the lung, stomach, and kidney of the mouse fetus at E17.5. These results suggest that NUCB2/nesfatin-1 may play an important role in liver and heart development and physiological functions in the developmental process of mouse fetus. Further studies are needed on the function of NUCB2/nesfatin-1, which is highly expressed in the various organs, including liver and heart during mouse development.

• 한국멀티미디어학회논문지
• /
• 제20권2호
• /
• pp.396-403
• /
• 2017

In recent years, the use of touch screens has expanded, and devices such as remote controllers have been developed in various ways to control and access contents at long range. The wireless-based touch screen is used in classroom, seminar room, and remote video conversation in addition to the TV remote control. The purpose of the study is to design a smart phone-based intuitive interface that can perform the role of a wireless mouse and a wireless keyboard at range using Bluetooth and to develop an application that integrates functions of a mouse and a keyboard. Firstly, touch interaction model for controlling software such as PowerPoint by connecting to a general PC on a smart phone has been studied. The most simple touch operation interface is used to reproduce the function of existing devices and design more simply. The studies of the extension of interfaces with various functions are important, but development of optimized interfaces for users will become more important in the future. In this sense, this study is valuable.

• Advances in Traditional Medicine
• /
• 제7권3호
• /
• pp.229-234
• /
• 2007

In macrophages, nitric oxide (NO) is released as an inflammatory mediator and has been proposed to be an important modulator of many pathophysiological conditions including inflammation. In this study, we have examined the inhibition effects of NO production by 85% methanol extract of the flower of Pueraria thunbergiana (PF) in mouse macrophages. Extract of PF (1, 10, 100 ${\mu}g/ml$) inhibited NO production, inducible NO synthase and cyclooxygenase-2 expression in interferon-g and lipopolysaccharide-stimulated mouse peritoneal macrophages and it had no cytotoxicity. These data suggest that 85% methanol extract of PF might be useful in controlling macrophages mediated inflammatory disease.

• 한국정보통신학회논문지
• /
• 제10권9호
• /
• pp.1671-1678
• /
• 2006

본 논문은 컴퓨터 모니터 거리 30-40cm에서 실시간 쌍안 눈동자 추적 마우스 시스템 구현에 관한 연구이다. 눈동자 탐색과 커서 의 추적과정은 소형 CCD카메라에서 받은 안면영상은 2진영상으로 변환되고 눈 주위의 5영역 매스크 방법으로 눈을 찾고 측4점 대각선위치 지 정 방법으로 각 홍채를 탐색한다. 커서추적은 홍채중심좌표의 이동위치를 측정하여 이루어진다. 화면커서 제어는 홍채의 최대 이동거리를 측정하여 화면커서최대 이동거리와 비교하고 홍채의 움직임에 비례해서 커서가 이동해야 하는 거리를 계산하여 화면커서를 화면의 바라보는 지점에 위치시킴으로써 화면커서가 제어된다. 실험 결과 구현시스템은 비교적 간단하고 실시간으로 동작속도가 빠름을 보였다.

• 한국수정란이식학회지
• /
• 제10권3호
• /
• pp.203-208
• /
• 1995

This study was carried out to efficiently use the ultrarapid freezing method in the cryopreservation of mouse ova. For this, the effects of dehydration method, oval vigour and $0^{\circ}C$ controlling method on post-thawing viability were investigated. Fresh mouse ova were dehydrated in mPBS with 3.5M DMSO and /or 0.25M sucrose, and directly immersed in L$N_2$ for ultrarapidly freezing. The frozen ova were thawed at 37$^{\circ}C$, rehydrated in mPBS with 0.25M sucrose, and then repeatedly washed in HAM's Fl0 before evaluating the morphological normality of frozen-thawed ova. The results obtained showed that there was difference between treatments in a experiment. 1) The post-thawing viability of ova dehydrated in multi-step (48.4$\pm$13.8%) was higher than that of ova in two-step (40.9$\pm$14.0%). 2) The post-thawing viability of fertilized ova (87$\pm$14.0%) was significantly(p<0.0l) higher than that of unfertilized ova (5.4$\pm$5.4%). 3) The post-thawing viability of ova dehydrated and rehydrated using a cooling machine (95.8$\pm$4.2%) was significantly(p<0.05) higher than that on ice(84.1$\pm$9.9). In conclusion, in order to efficiently cryopreserve ova in vitro with ultrarapidly freezing method, highly viable embryos should be selected, heavy osmotic shock to the dehydrating ova should be avoided, and embryos in high osmotic condition were dehydrated and rehydrated in a constantly low temperature.

• 정보과학회 컴퓨팅의 실제 논문지
• /
• 제21권8호
• /
• pp.531-536
• /
• 2015

음성인식은 HCI(Human Computer Interface)분야에서 가장 활발히 연구되고 있는 분야로 음성을 이용하여 디지털 디바이스를 제어하는 것을 목적으로 하고 있으며 마우스는 GUI 컴퓨터 환경에서 가장 널리 사용하는 장치로서 높은 보급률을 자랑하는 컴퓨터 주변기기 중의 하나이다. 본 논문은 스마트폰 환경에서 실시간 모음 음성 인식을 이용한 마우스 제어 방법에 관하여 제안한다. 구현 방법은 스마트폰에서 실시간으로 일정크기의 음성 신호를 입력 받아 핵심 음성 신호를 추출하고 MFCC(Mel Frequency Cepstral Coefficient)를 이용하여 특징을 추출하여 학습되어 있는 코드 북을 이용하여 양자화를 진행하고 HMM(Hidden Markov Model)을 이용하여 해당 모음 단어를 인식한다. 그리고 각 모음에 해당하는 마우스 명령어로 변환하여 화면상의 가상의 마우스를 제어한다. 최종적으로, 우리는 구현된 스마트폰의 앱을 가지고 데스크톱 PC의 화면상에서 다양한 마우스의 동작을 보여준다.

• Advances in Traditional Medicine
• /
• 제6권3호
• /
• pp.161-166
• /
• 2006

Chrysanthemum morifolium (CM) is a herb widely used in medicine for the treatment of a variety of diseases. In this study, using mouse peritoneal macrophages, we have examined whether CM affects nitric oxide (NO), tumor necrosis factor $(TNF)-\alpha$ and interleukin (IL)-6 induced interferon $(IFN)-\gamma$ and lipopolysaccharide (LPS). CM inhibits $IFN-\gamma$ and LPS-induced NO in dose dependent manner. We also found that CM inhibits pro-inflammatory cytokine, $TNF-\alpha$ and IL-6. The expression of cyclooxygenase-2 was reduced by CM. These finding means that CM can be used in controlling macrophages-mediated inflammatory disease.