• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.151-162
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• 2001

산삼은 고유의 생약으로 민간 또는 한방에서 효능을 인정받아 왔으나 산삼의 희귀성으로 인하여 산삼에 대한 연구가 활발하지 못하였다. 따라서 본 실험은 식물 조직 배양 기술을 이용하여 산삼 부정근을 대량으로 배양하고 추출하여 화장품 원료로서의 적용 가능성을 연구하였다.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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• pp.611-616
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• 2003

Korean ginseng(Panax Ginseng C.A. Meyer) known as a oriental miracle drug is an important medicinal plant. Ginseng has been used for geriatric, tonic, stomachic, and aphrodisiac treatments for thousands years. Also, it is an antibiotic and has therapeutic properties against stress and cancer. Ginseng is widely distributed all over the world. Among them, Korean mountain ginseng has the most valuable effect on pharmaceuticals. The roots of mountain ginseng contained several kinds of ginsenosides that have many active functions for the human body. However, the study of mountain ginseng has a limit because the mountain ginseng is very expensive and rare. So, we artificially cultured mountain ginseng adventitious roots using the bioreactor culture system. We induced callus from original mountain ginseng, directly dug up in mountain and aged about one hundred ten years. Separated adventitious roots were precultured in 500ml conical flasks and then, transferred in 20L bioreactors. The adventitious roots of mountain ginseng were harvested after culturing for 40days, dried and then, extracted with several solvents. In this study, we investigated the whitening effect, anti-wrinkle effect and the safety of tissue cultured adventitious roots extract of mountain ginseng in order to identify the merit as a cosmetic ingredient. Particularly, extract of mountain ginseng adventitious roots showed whitening and anti-wrinkle effects. The inhibitory effect of this extract on the melanogenesis was examined using B-16 melanoma cell. When B-16 melanoma cells were cultured with adventitious root extract, there was a dramatically decrease in melanin contents of 8-16 melanoma cell. And we identified this extract inhibited Dopa auto-oxidation significantly. Also, when transformed mouse fibroblast L929 cells were treated with this extract, there was a significant increase in collagen synthesis. The results show significant inhibited melanization and wrinkle without inhibiting cell viability.

• 대기
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• 제17권3호
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• pp.231-240
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• 2007

The flow regimes of continuously stratified flow over a double mountain and the effects of a double mountain on wave breaking, upstream blocking, and severe downslope windstorms are investigated using a mesoscale numerical model (ARPS). According to the occurrence or non-occurrence of wave breaking and upstream blocking, three different flow regimes are identified over a double mountain. Higher critical Froude numbers are required for wave breaking and upstream blocking initiation for a double mountain than for an isolated mountain. This means that the nonlinearity and blocking effect for a double mountain is larger than that for an isolated mountain. As the separation distance between two mountains decreases, the degree of flow nonlinearity increases, while the blocking effect decreases. A rapid increase of the surface horizontal velocity downwind of each mountain near the critical mountain height for wave breaking initiation indicates that severe downslope windstorms are enhanced by wave breaking. For the flow with wave breaking, the numerically calculated surface drag is much larger than theoretically calculated one because the region with the maximum negative perturbation pressure moves from the top to the downwind slope of each mountain as the internal jump propagating downwind occurs.

• 대기
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• 제19권4호
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• pp.345-370
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• 2009

Numerical experiments using the Weather Research and Forecasting (WRF) model were done to identify the role of the mountain ranges in the northern part of the Peninsula (referred as "the northern mountain complex"), in the occurrence of two heavy snowfall events over the Yeongdong region on 7-8 December 2002 and 20-21 January 2008. To this end, control simulations with the topography of the northern mountain complex and other simulations without the topography of the mountain complex were performed. It was revealed that the amount of snowfall over the Yeongdong region from the control simulation much more exceeded that of the simulation without the topography of the mountain complex. This increase of the snowfall amount over the Yeongdong region can be explained as follows: As the upstream flow approached the northern mountain complex, it deflected around the northern mountain complex due to the blocking effect of the mountains with a low Froude number less than ~0.16. This lead to the strengthening of northeasterly over the East Sea and over the Yeongdong region. The strong northeasterly is accompanied with much more snowfall over the Yeongdong region by intensifying air-mass modification over the sea and the orographic effect of the Taeback mountains. Thus, it was concluded that the topography of the northern mountain complex is one of the main factors in determining the distribution and amount of precipitation in the Yeongdong region when there is an expansion of the Siberian High toward the East Sea.

• 한국의류학회지
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• 제37권4호
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• pp.565-576
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• 2013

This study investigated 1) the effects of mountain climbing motivation on the desired mountain climbing wear benefits, and 2) the desired effects of mountain climbing wear benefits on clothing usage behavior. Data were collected via a questionnaire administered to 498 consumers who make at least one mountain climbing wear purchase for personal use. All items were evaluated on a 5-point Likert scale, and SPSS 20.0 and Amos 7.0 were used for data analysis. Confirmatory factor analysis for the research variables were performed and three factors of mountain climbing motivation (social interaction, health improvement, and mental rejuvenation), four factors of mountain climbing wear benefits sought (ostentation, ideal healthy figure, conformity, and functions), and two factors regarding clothing usage behavior (sportswear usage and daily wear usage) were identified. The results from the model test were as follows: 1) Mountain climbing motivation was found to vary depending on the mountain climbing wear benefits sought. Social interaction had a positive effect on all 4 factors of mountain climbing wear benefits sought. Health improvement was the only motivating factor for consumers who sought the benefit of functions; however, the functions benefit was not found to be a consideration for consumers who climbed for mental rejuvenation. 2) Mountain climbing wear usage was affected by mountain climbing wear benefits sought. The benefits for conformity and functions had a positive effect on sportswear usage and daily wear usage; however, the ideal healthy figure only affected daily wear usage. The twofold usage of mountain climbing wear reflects a cultural trend toward the usage of mountain climbing wear as daily wear; however, consumers show this twofold usage to conform successfully to mainstream society and not to pursue the ideal healthy figure.

• 대한화장품학회지
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• 제30권3호
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• pp.377-383
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• 2004

본 연구는 식물조직배양 기술을 이용하여 대량으로 배양된 산삼부정근의 추출물에 대한 화장품 원료로서의 응용 가능성을 검토하였다. 본 실험에서 사용된 산삼부정근은 강원도 평창에서 채취한 110년생 천종산삼으로부터 유래된 캘러스에서 부정근을 유도한 후 절취하여 생물 배양기에서 액체 현탁액으로 대량 배양시켰다. 약 5주간의 배양기간을 거쳐 증식된 산삼부정근을 세척하고 건조시킨 후 추출하여 산삼부정근 추출물을 얻었다 조직 배양된 산삼 부정근 추출물의 in vivo에서의 미백 효과를 검증하기 위하여 조직배양된 산삼부정근 추출물을 함유한 제형을 이용한 미백 임상실험을 실시하였다. 그 결과 산삼부정근 추출물을 함유한 제형에서 두드러진 미백효과를 보여주었으며 통계적으로도 유의차(p<0.0001)를 보여주었다. 그러나 산삼의 주요 성분인 일부 saponin에 대한 tyrosinase 억제 실험 및 B-16 melanoma를 이용한 미백 실험을 실시한 결과 미백효과가 $10\%$ 이하로 낮게 나왔으며 인삼 및 홍삼 추출물의 경우에서도 산삼부정근 추출물과 같은 농도에서는 거의 미백 효과가 관찰되지 않았다. 또한 항산화 효과를 알아보기 위한 DPPH 실험에서는 산삼부정근 추출물이 $0.05\%$ 농도에서 $85\%$ hydroxyl radical 소거능을 보여주었다.

• 대한약침학회지
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• 제14권1호
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• pp.35-49
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• 2011

Objectives: The aim of this experiment is to know about Effect of pulse-wave factors in Middle Aged Women by Mountain Cultivated Ginseng Pharmacopuncture. Methods: First 20 Middle Aged women are diagnosed by pulse diagnosis, and then Mountain Cultivated Ginseng Pharmacopuncture(1 injection 20cc) were injected. 30 minutes later, pulse diagnosis again performed. As a result, method of one-group pretest-posttes design were used for evaluation. Results: T(Total pulse cycle) time statistically significant increased on both left and right chon, kwan, cheok. T4 time statistically significant increased on both left and right chon, kwan, cheok. T4-T1/T indexs except left cheokmaek, Right cheokmaek observation area decreased significantly in four sites. Wm(indicating high pressure retention time) indexs increased significantly in the five sites were observed except right chon maek. Conclusions: Effect of pulse-wave factors in Middle Aged Women by Mountain Cultivated Ginseng Pharmacopuncture increased T, T4, Wm and decreased T4-T1/T indexs. The results of this experiment, Mountain Cultivated Ginseng Pharmacopuncture induced to increase the Pulse-wave's stability and strength.

• 한국농촌계획학회:학술대회논문집
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• 한국농촌계획학회 1998년도 임시총회 및 추계 학술논문발표회
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• pp.22-24
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• 1998

This is a research with two purposes, in order to understand the situation with which mountain villages region gets faced present time. First, to examine the conception and the formative process of mountain villages region. Second, the measure the possibility of development in a project on mountain villages region to increase the evenings as a continuous project by bringing ecology tourism. The future plan for mountain villages region project will focus on building up natural resting places for citizens by offering the actual living area it self mountain villages region should be made a place where people can release fatigue or stress, rest, think, have fun and get relationship each other, villagers and citizens. For this, it, is needed to find the way to increase economic effect in mountain villages region after enough study of development peculiarity each mountain villages region has, propensity to consume, appropritate method and example, negative and positive influence.

• 한국환경과학회지
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• 제12권3호
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• pp.247-255
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• 2003

Forest management starts from forest road facility, which is designated as generation source of muddy water in mountain stream during initial stage of establishment. Therefore, this study reviewed the effect of suspended sediment generated in forest road surface on the muddy water in mountain stream with respect to marsh area of forest. As a result, characteristics of outflow of suspended sediment was understood, and it was judged that generation of suspended sediment due to establishment of forest road is diluted by mountain stream this charged from drainage area so as to have small effect on muddy water in total mountain stream.

• 대한화장품학회지
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• 제27권2호
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• pp.45-56
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• 2001

산삼은 고유의 생약으로 민간 또는 한방에서 효능을 인정받아 왔으나 산삼의 희귀성으로 인하여 산삼에 대한 연구가 활발하지 못하였다. 따라서 본 실험은 식물 조직 배양 기술을 이용하여 산삼 부정근을 대량으로 배양하고 추출하여 화장품 원료로서의 적용 가능성을 연구하였다. 본 실험에서 사용한 산삼 부정근은 강원도 평창에서 채취한 110년생 천종삼으로 산삼으로부터 유래된 캘러스에서 부정근을 유도한 후 절취하여 생물 배양기에서 액체 현탁액으로 대량 배양하였다. 약 5주간의 배양기간을 거쳐 증식된 산삼 부정근을 세척하여 건조시킨 후 추출하여 산삼 부정근 추출물을 얻었다. 산삼 부정근 추출물의 미백 효과 측정을 위하여 tyrosinase 억제 실험과 DOPA 자동산화 그리고 B-16 melanoma cell를 이용한 미백 실험을 실시하였고 유해성 검증을 위해서 안전성 실험과 세포 독성 실험을 실시하였다. in vitro 상의 유해성 실험은 transformed mouse fibroblast L929를 배양하여 NR assay, MTT assay를, in vivo 상의 안전성 실험은 인체 첩포를 이용한 Patch test를 실시하여 피부 반응의 관찰을 통해서 자극 혹은 알레르기성 반응의 발생여부를 실시한 결과 무해하였으며 melanin 생성 억제 실험 결과 미백효과가 우수한 것으로 나타났다.