• Journal of Popular Narrative
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• v.27 no.2
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• pp.13-44
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• 2021

This article describes the fear of infection through the Covid19 pandemic and the rapid phase change of human species with H. P. Lovecraft's fiction and "Project LC. RC". Pandemic and climate change, which can be called global weirding, fundamentally question the status and history of human species in the ecosystem. The horror creature and cosmological indifferentism in Lovecraft's weird fiction are contemporary in that they help shed light on today's global weirding. But Lovecraft's racism allows him to ask more fundamental questions about the logjam of his cosmic horror. "Project LC. RC" are a Korean writers's works of cultural variation that rewrites controversial racism and misogyny in Lovecraft's fiction. Such variation becomes the task of creating a mutation in Lovecraft as it becomes infected with the affection of Lovecraft's writing. This article first noted the creative power of Lovecraft's fiction that induces such a mutation. And under this premise, this article wanted to reveal the meaning of Lee Seo young, Eun rim, and Kim Bo young's recreates of Lovecraft's fiction through the analysis of images and motifs of abject, plant creature and symbiosis. Specifically, Lovecraft's creature, which evokes phallic fear, turns into an image of an abject embracing and comforting women's despair("I Want You to Stay Low"), a plant creature that provides women with refuge("Color in the Well"), and a creature of care and symbiotic life("A Sea of Plague"). This recreate/rewriting has contemporary significance in that it embodies values such as labor, care, and solidarity in their works. The conclusion noted another power of creative variation in Lovecraft's fiction, which is not reduced to recreate/rewriting.

• Journal of the Korean Society of Floral Art and Design
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• no.45
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• pp.31-46
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• 2021

With the advent of the post-COVID19 era, people must maintain social distancing to quarantine. However, this rule deprives people of freedom. Therefore, this study proposes a new normal plan for urban park design using drive-thru to recreate space for people to maintain and enjoy their previous lives while complying with quarantine rules. Olympic Park has a large floating population, and is one of the places where drive-thru is available. Therefore, the study designed this place to be the only cafe that could be operated if other cafes were shut down due to social distancing. The cafes in the park were designed into five spaces based on Olympic Park's flag motifs. The results were as follows. The cafe's name is CUPPY (Cup+Coffee), while each logo letter is expressed using the colors of the Olympic flag: blue, yellow, black, green, and red. The cafe spaces were divided into five continents (Europe, Asia, Africa, Oceania, and America), as symbolized by the Olympic flag, with the driving route shaped like the Olympic logo to match the five spaces. Human beings need change and adaptation in various fields to live in a post-COVID19 era that they have never experienced before. Just as the New Normal changes with time, and should, research is essential for presenting a New Normal in urban park design that reflects this disaster situation following the COVID-19 crisis. On this very point, we expect that this research will serve as a reference for urban park design. Additionally, it is believed that continuous suggestions and research will be necessary to apply the model to more diverse environments.

• Development and Reproduction
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• v.12 no.3
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• pp.297-303
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• 2008

BCL-2 family members are essential protein for the regulation of cell death and survival consisting both antiapoptotic and pro-apoptotic proteins. In the present study, we designed and cloned a new apoptotic molecule MCL-1ES BH3M coding a modified protein of MCL-1L. Compared to MCL-1L protein, MCL-1ES BH3M lacks the PEST motifs known to be involved in MCL-1L protein degradation and has seven mutated residues in BH3 domain critical for dimerization with BCL-2 family members. Overexpression of MCL-1ES BH3M induced death of different cells, and its cell killing effect was not blocked by forced expression of the pro-survival protein MCL-1L. Expression of MCL-1ES BH3M protein led to the activation of caspase 9 and caspase 3, suggesting apoptotic cell death, and confocal fluorescent microscopic analyses showed that MCL-1ES BH3M was partially localized in mitochondria. In conclusion, we reported a new apoptotic molecule and determined its cell death activity in cells.

• Journal of Life Science
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• v.33 no.1
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• pp.8-14
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• 2023

Peroxidasin (PXDN), a multidomain heme peroxidase containing extracellular matrix (ECM) motifs, as well as a catalytic domain, catalyzes the sulfilimine crosslink of collagen IV (Col IV) to reinforce Col IV scaffolds. We previously reported that PXDN is required for endothelial cell (EC) survival and growth signaling through sulfilimine crosslink-dependent matrix assembly. In this study, we examined whether peroxidase activity is required for PXDN function in ECs. First, we constructed a mutant PXDN by point mutation of two highly conserved amino acids, Q823 and D826, which are present in the active site of the peroxidase domain. After isolation of HEK293 clones highly expressing the mutant protein, conditioned medium (CM) was obtained after incubating the cells in serum-free medium for 24 hours and then analyzed by Western blot analysis under nonreducing conditions. The results revealed that the mutant PXDN formed a trimer and that it was cleaved by proprotein convertase-like wild-type (WT) PXDN. However, peroxidase activity was not detected in the CM containing the mutant PXDN, in contrast to that of WT PXDN. In addition, the sulfilimine crosslink ability of the mutant PXDN was lost. Moreover, the CM containing the mutant PXDN failed to promote the growth of PXDN-depleted ECs, unlike the CM containing WT PXDN. These results suggest that the peroxidase activity of PXDN affects EC growth by forming a sulfilimine crosslink.

• Journal of the Korean Institute of Rural Architecture
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• v.25 no.2
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• pp.1-8
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• 2023

This study reveals the absence of standard design guidelines by region through investigation and analysis centered on public design cases by region in Jeollabuk-do and design elements that can reflect the integration, identity, and diversity of public design in each region by five regions. Through this, the following conclusions could be obtained. First, to improve the quality of street facilities in Jeollabuk-do, the design elements (design motif, color, pattern) applicable to the standard design were analyzed by dividing them into five regions. As a design motif, it was possible to extract patterns containing straight lines, sophistication, dignity, and smartness. In the Northeast region, it is comfortable with the motif of the mountain ridge reflecting geographical characteristics, and it can be extracted elements that contain warm and natural colors. In the southeastern region, patterns that reflect design elements were extracted by applying safe, lively, and peaceful colors with the design motif of curves that blend nature and agriculture. In the southwestern region, design pattern elements that highlight nature, history, and culture were extracted with various cultural assets and natural greenery as motifs. Lastly, in the Saemangeum region, the ocean flow and greenery could be used as a design motif to reflect a positive, clear, future-oriented image in the design spot zones by region. Second, based on the standard design elements (design motive, color, pattern) by region extracted for the standard design development of street facilities in each region in Jeollabuk-do, an integrated zone(Form, structure, material, color, functional element) to which regional design guidelines can be applied. Third, an integrated zone (form, structure, material, color, functional elements) was composed. In addition, design spot zones (patterns and colors in city and county units) that can contain the diversity and identity of each region were designated. By designating design spot zones (patterns and colors in city and county units) that can contain the diversity and identity of each region, standard design development plans (integrated pillars, jaywalking prevention fences, roundabouts (urban type, rural type), street trees) Eight standard designs, including protective covers, street planters, flat benches, light benches, visual media for user guidance, and parking zones for personal mobile devices) were presented.