• Toxicological Research
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• 제11권2호
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• pp.241-246
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• 1995

This study was carried out to develop the antitoxic compound about cytotoxicity of cadmium on cultured rat neuroglial cells. These cells divided into 3 groups; control group (medium only) or $MTT_{50}$ group (neuroglial cell, $61{\mu}M$ cadmium) and experimental group ($61{\mu}M$ caffeic acid). Neutral red (NR) and tetrazolium MTT of the colorimetric assay were performed to evaluate the cytotoxicity of cell organelles. The light microscopic study was carried out to morphological changes of cultured rat neuroglical cells. The results indicated that caffeic acid showed detoxification effect on cytotoxicity of cadmium in $61{\mu}M$ concentration. According to the spectroscopic study of 1:1 complex of cadmium and caffeic acid, it showed that this formation of complex eliminated cadmium from cultured rat neurogllal cells.

• Parasites, Hosts and Diseases
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• 제45권3호
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• pp.191-198
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• 2007

A total of 205 larval gnathostomes were collected from 18 (22.5%) of 80 red banded odd-tooth snakes, Dinodon rufozonatum rufozonatum, which had been smuggled from China and confiscated at Customs in Susan, Republic of Korea. In order to identify the species, some of the larvae were observed by a light microscope and a scanning electron microscope (SEM). The larvae were $2.18{\times}0.29mm$ in average size, and had a pair of lips at the anterior end, a muscular esophagus, 2 pairs of cervical sacs, and brownish intestines. The head bulb was characteristically equipped with 4 rows of hooklets; the average number of hooklets in each respective row was 38.6, 40.5, 41.5, and 43.7. In SEM views, the mouth evidenced a pair of lateral lips of equal size in a half-moon shape. Each lip featured a couple of labial papillae and a small amphid located between the 2 papillae. The hooklets on the head bulb had single-pointed, posteriorly-curved tips. The cuticular spines were larger and more densely distributed on the anterior part of the body, and decreased gradually in size and number toward the posterior body. On the basis of these morphological characteristics, the larvae were identified as the third stage larvae of Gnathostoma hispidum.

• Parasites, Hosts and Diseases
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• 제51권2호
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• pp.259-263
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• 2013

Human taeniasis was investigated in Lao People's Democratic Republic (Lao PDR) between 2000 and 2011 as part of the nation's helminthiasis survey. A total of 55,038 inhabitants, including 29,846 school children, were examined using the Kato-Katz and scotch-tape anal swab method, and morphological observation of adult worms. Molecular identification of Taenia tapeworms was performed by multiplex PCR or DNA sequence analysis of the mitochondrial cox1 gene. Taenia eggs were present at a rate of 1.5% (845/55,038) in the subject population. Adult tapeworms were identified as T. solium or T. saginata by analyzing the collectable stool specimens (n=126). Three specimens identified as T. solium were found in Luang Prabang, while the remaining 123 specimens, which were T. saginata, were found in Bokeo, Bolikhamxay, Champasak, Houaphan, Khammouane, Luang Namta, Luang Prabang, Oudomxay, Phongsaly, Saysomboune, Saravane, Savannakhet, Xayaboury, Xekong, Xieng Khouang Province, and Vientiane Municipality.

• Parasites, Hosts and Diseases
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• 제55권5호
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• pp.575-578
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• 2017

Aspidogastrid trematodes (Subclass Aspidogastrea) are a relatively small group with a characteristic adhesive disc and parasitize in a variety of cold-blooded hosts. Until now, only 2 species in the genus Cotylaspis, i.e., C. coreensis and C. sinensis, have been reported as the aspidogastrid trematode in the Republic of Korea (=Korea). In the present study, we intended to describe a species of aspidogastrids collected from the small intestine of the common carp, Cyprinus carpio, in a faunistic point of view. Total 51 specimens were collected from a carp caught in Nakdong-gang (River) on May 2015. Some of them were prepared as the specimens for light microscopic observations, and some others were prepared for SEM. They were slightly elongated without head lobes, $2,432{\times}840{\mu}m$ in average size, and had characteristic adhesive discs with 4 rows and 46 alveoli in average. The ovary was reniform and was located in the posterior-upper part of the body. The single testis was larger than the ovary and was located below the ovary. The uterus was coiled containing numerous eggs and distributed in the posterior 2/3 of the body. The vitellaria were follicular, and distributed from the mid-level of testis to near the posterior end. The morphological characters with dimensions of our specimens were closely identical with those of Aspidogaster ijimai previously described. A new aspidogastrid is added among the Korean trematode fauna by the present study.

• 대한수의학회지
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• 제38권3호
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• pp.497-507
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• 1998

The localizations and morphological characteristics of immunoreactive cells for prolactin(PRL) and growth hormone(GH) antisera were studied with double immunohistochemistry in the adenohypophysis of the Korean native goat. PRL immunoreactive cells(mammotropes) and GH immunoreactive cells(somatotropes) were present in the pars distalis and pars tuberalis, but not in the pars intermedia. A few cells were stained with both PRL and GH antisera (somatomammotropes). The possessional percentages of mammotropes, somatotropes and somatomammotropes were 37.0%, 32.6%, 1.0% in females and 35.6%, 32.6%, 1.1% in males, respectively. Mammotropes were oval or round in shape, and $10{\sim}20{\mu}m{\times}17{\sim}25{\mu}m$ in size. These cells were distributed throughout the pars distalis, but were more abundant on the dorsal part adjacent to the hypophyseal cavity and along the lateral and ventral peripheral regions. Somatotropes were elliptical, triangular or polygonal in shape, and $8{\sim}17{\mu}m{\times}17{\sim}18{\mu}m$ in size. The distribution pattern of somatotropes was similar to that of mammotropes. Some somatomammotropes were intercalated between clusters of mammotropes while the others were dispersed independently among the secretory cells.

• 대한수의학회지
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• 제39권5호
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• pp.855-864
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• 1999

Hemal nodes and hemolymph nodes are lymphoid organs that share morphologic and functional characteristics of lymph nodes and spleens. The aim of the present study was to obtain new informations on the distinct morphological structures of hemal nodes and hemolymph nodes according to ages, and to get the basic data for their functions in Korean native goats. Goats were divided into 5 groups, consisting of 3 animals aged 1, 3, 6, 10 and 12 months, respectively. Ultrastructural features of the organs were observed by transmission and scanning electron microscopes. The sinuses of hemal nodes and hemolymph nodes were lined by endothelial-like reticular cells which had euchromatin-rich nuclei and many cytoplasmic processes, surrounding collagen fibrils. Macrophages containing phagocytosed erythrocytes were often noted in the diffuse lymphatic tissues of hemal nodes and hemolymph nodes. Some mast cells were in contact with the plasma cells near the blood vessel. Hemal nodes and hemolymph nodes had venous sinusal-like vessels which were different from the deep sinus. The lymph vessels with valves were observed in the capsule of the hemolymph node. There were no ultrastructural differences of the organs in the age different groups of the animals. These results suggest that hemal nodes and hemolymph nodes may take part in hemopoiesis, blood filtration and immune reaction in Korean native goats.

• 한국식품위생안전성학회지
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• 제26권4호
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• pp.361-365
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• 2011

To evaluate the indoor air quality of food manufacturing plants, the presence of viable bacteria and fungi was assessed in the indoor air of the facilities at which 9 food items were manufactured. Air samples were collected from the general zone, low clean zone and clean zone of each factory with an air sampler, in combination with plate counts agar using for bacteria, and dichloran-glycerol agar for fungi. The samples were incubated at $25^{\circ}C$ for 4 to 7 days. After culture, the colony forming units (CFU) on each plate were counted and corrected with a positive hole conversion table. The average concentration of bacteria was $2.2{\times}10^3\;CFU/m^3$ in the general zone, $1.2{\times}10^3\;CFU/m^3$ in the low clean zone and $7.3{\times}10^2\;CFU/m^3$ in the clean zone. The average concentration of fungal microbes was $2.5{\times}10^3\;CFU/m^3$ in the general zone, $2.6{\times}10^3\;CFU/m^3$ in the low clean zone, and $2.0{\times}10^2\;CFU/m^3$ in the clean zone. No meaningful differences were detected between the general zone and the low clean zone, but the clean zone had significantly lower concentrations than the other zones. Additionally, the identification of the fungi was performed according to morphological method using a giant culture and slide culture. The fungi were identified as belonging to 18 genera, and the genera Cladosporium(33%), Penicillium(29%) and Aspergillus(26%), predominated. Aspergillus isolates were identified to species level, and A. ochraceus, a mycotoxigenic species, was identified. As part of the effort to control the quality of the indoor air of food manufacturing plants, our results show that continued studies are clearly warranted.

• 한국수자원학회논문집
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• 제37권1호
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• pp.55-66
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• 2004

황강 상류에 위치한 합천댐은 1988년 12월에 완공되었다. 완공 이후 댐의 저수량 부족으로 10여년간 수문을 통한 방류가 없었기 때문에, 댐 하류부에 새로운 생태계가 조성되었다. 이러한 현상은 국내 다른 지역에서 찾아보기 힘든 매우 특이한 사항이다. 본 연구에서는 댐 건설로 인한 유황변화에 따른 하천의 지형학적 변화와 식생 피복상태의 변화에 대한 분석을 수행하였다. 이를 위해 하상고 및 하천수로단면의 변화를 조사하였다. 또한 나이테 분석을 포함한 현지조사를 수행하였다. 또한 댐 건설 전ㆍ후 대상유역의 항공사진을 비교하여 댐 건설 후 식생피복이 증가한 것을 확인하였다.

• 한국콘텐츠학회논문지
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• 제7권6호
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• pp.60-68
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• 2007

언어 정보 처리 응용 분야는 정보검색, 형태소분석, 철자검색, 음성인식, 문자 인식 등 다양하다. 이러한 정보처리 과정은 전자 사전이 필수적이다. 본 논문에서는 국어대사전에 대한 기본적인 통계 정보들을 살펴보고, 전자사전 구축에 대하여 알아보았다. 대상 정보는 고어 및 불완전음절을 포함하는 단어를 제외한 표제어들에 대하여, 대사전의 표제어수, 전자사전의 엔트리수, 사용된 전체음절수, 서로 다른 음절수, 엔트리들의 평균 길이, 품사별 분포, 전자사전을 트라이로 구축할 때 사용되는 노드 수 등 이다. 전자사전의 전체 엔트리 수는 361,980개, 사용된 음절수는 1,289,659개로 엔트리들의 평균 길이는 3.56이었으며 서로 다른 음절수는 2,463개였다. 이러한 통계 정보들은 전자사전 구축 및 한국어 정보처리에 도움이 될 것이다.

• 한국정밀공학회지
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• 제23권2호
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• pp.154-163
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• 2006

Tooth morphology is the most important scientific aspect of dental medicine in regards to the treatment and study of teeth attrition relating to the absence of teeth due to dental caries or the occlusion of teeth due to external force. Most of the studies have focused on the external morphology in cutting teeth regardless of sex and age. However, the importance of internal morphology in the treatment of damaged teeth has been increased. Therefore, this study established the measurement criteria for the morphology of the mandibular first premolar which had never been presented, in order to investigate the external and internal morphologies of mandibular first premolars, and introduced a non-destructive method such as a microcomputed tomogrphy. Mandibular first premolars in superlative state were taken from molars of middle aged males and females and used as specimens for this study. Criteria relating to the internal and external morphology measurements were established to quantify the length of the teeth in identical state. Two dimensional image data for the selected mandibular first premolar were obtained by taking the image of each O.022mm section, which is perpendicular to the vertical direction using the microcomputed tomography. The Vworks program was applied to measure the length of each morphological part according to the set measurement criteria. These measured data were compared with the data presented by G. V. Black and the internal and external morphologies of the teeth of middle aged Koreans were also compared according to gender. In addition, the methodology for measurement of the mandibular first premolar was presented and according to this, the standardized mandibular first premolars of middle aged Korean males and females were made by using a rapid prototyping system.