Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Development of Antitioxic Agents from Korean Medicinal Plants. Part 5. Antitoxic Effects of Binding of Caffeic acid and Cadmium on Cultured Rat Neuroglial Cells

한국산 생약으로부터 해독물질의 개발(제5보) 배양 백서 신경아교세포에서 Caffeic acid와 카드뮴의 결합에 의한 해독효과

  • Baek, Seung-Hwa (Dept. of Chemistry, College of Natural Science, WonKwang University) ;
  • Lee, Hong (Dept. of Chemistry, College of Natural Science, WonKwang University) ;
  • Pae, Hyun-Ock (Dept. of Chemistry, College of Natural Science, WonKwang University) ;
  • Kim, Young-Ok (Dept. of Chemistry, College of Natural Science, WonKwang University) ;
  • Kwak, Jung-Suk (Dept. of Chemistry, College of Natural Science, WonKwang University) ;
  • Yoo, Hyeon-Gyeong (Dept. of Oral Anatomy, College of Dentistry, WonKwang University) ;
  • Han, Du-Seok (Dept. of Oral Anatomy, College of Dentistry, WonKwang University)
  • 백승화 (원광대학교 자연과학대학 화학과) ;
  • 이홍 (원광대학교 자연과학대학 화학과) ;
  • 배현옥 (원광대학교 자연과학대학 화학과) ;
  • 김영옥 (원광대학교 자연과학대학 화학과) ;
  • 곽정숙 (원광대학교 자연과학대학 화학과) ;
  • 유현경 (원광대학교 치과대학 구강해부학교실) ;
  • 한두석 (원광대학교 치과대학 구강해부학교실)
  • Published : 1995.12.01
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Abstract

This study was carried out to develop the antitoxic compound about cytotoxicity of cadmium on cultured rat neuroglial cells. These cells divided into 3 groups; control group (medium only) or $MTT_{50}$ group (neuroglial cell, $61{\mu}M$ cadmium) and experimental group ($61{\mu}M$ caffeic acid). Neutral red (NR) and tetrazolium MTT of the colorimetric assay were performed to evaluate the cytotoxicity of cell organelles. The light microscopic study was carried out to morphological changes of cultured rat neuroglical cells. The results indicated that caffeic acid showed detoxification effect on cytotoxicity of cadmium in $61{\mu}M$ concentration. According to the spectroscopic study of 1:1 complex of cadmium and caffeic acid, it showed that this formation of complex eliminated cadmium from cultured rat neurogllal cells.

Keywords

References

  1. Toxicology v.62 no.2 The cytotoxic effects of cadmium chloride and mercuric chloride mixtures in rat primary hepatocyte cultures Beattie,J.H.;Marion,M.;Schmit,J.P.;Denizeau,F.
  2. Toxicology. v.28 Cellular adaption in metal toxicology and metallothionein Cherian,M.G.;Nordberg,M.
  3. Chem. Biol. Int. v.84 Effect of caffeic acid ester on carcinogen induced mutagenicity and human colon adenocarcinoma cell growth Chinthalapally,V.R.;Dhimant,D.;Bipin,K.;Shantu,A.;Bandara,S.R.
  4. Toxicology and Applied Pharmacology v.113 no.2 Cadmium-induced DNA strand damage in cultured liver cells : reduction in cadmium genotoxicity following zinc pretreatment Coogan,T.P.;Bare,R.M.;Waalkes,M.P.
  5. Teratology v.28 The Effect of the time of administration of chromium trioxide on the embryotoxic response in hamsters Gale,T.F.;Bunch,J.D.
  6. Teratology v.28 Methylmercury toxicity in the chick embryo Greener,Y.;Kochen,J.A.
  7. Cancer Res. v.48 Inhibitory effect of carcumin, chlorogenic acid, caffeic acid and ferulic acid on tumorpromotion in mouse skin by 12-o-tetradecannoyl phorbol-13-acetate Huang,M.T.;Smart,R.C.;Wang,C.Q.;Conney,A.H.
  8. Toxicology Letters. v.51 no.1 Cadmium cytotoxity correlates with the changes in glutathione content that occur during the logarithmic growth phase of A549-T27 cels Kang,Y.J.;Enger,M.D.
  9. Toxicology Letters v.63 no.1 Protective effect of copper against cadmium cytotoxicity on cultured valscular endothelial cells Kaji,T.;Fujiwara,Y.;Koyangi,E.;Yamamoto,C.;Mishima,A.;Sakamoto,M.;Kozuka,H.
  10. Toxicology v.76 no.3 Possible mechanism for zinc protection against cadmium cytotoxicity on cultured valscular endothelial cells Kaji,T.;Ohkaward,S.;Yamamoto,C.;Sakamoto,M.;Dozuka,H.
  11. Cancer Res. v.52 no.7 Establishment and characterization of an in vitro model of acquired resistance to cisplatin in a human testicular nonseminomatous germ cell line Kelland,L.R.;Mistry,P.;Avel,G.;Freidlos,F.;Loh,S.Y.;Roberts,J.J.;Harrap,K.R.
  12. J. Kor. Chem. Soc. v.36 Thermodynamic infrared and magnetic studies on the complexation of Ge (III) by benzonate anion Kim,Y.I.;Park,H.J.;Choi,S.N.;Yun,S.S.
  13. Toxicology v.77 no.1-2 Alterations in cytoskeketak oriteub sulfhydryl and cellular glutathione in cultured cells exposed to cadmium and nickel ions. Li.W.;Zhao,Y.;Chou,I.N.
  14. Environmental and Molecular Mutageneses v.23 no.2 Studies on cytotoxic and genotoxic effects of cadmium nitrate and lead nitrate in chinses hamster ovary cells Lin,R.H.;Lee,C.H.;Lin Shiau,S.P.
  15. In vitro cellular and Developmental Biology-Animal v.26 no.1 Rat primary hepatocyte cultures are a good model for examining metallothionein-induced tolerance to cadmium toxicity Liu,J.;Kershaw,W.C.;Klaassen,C.D.
  16. Sci. Total Environ. v.14 Recent progress and perspectives in cadmium health effects studies Nomiyama,K.
  17. Mut. Res. v.274 no.3 Increased sensitivity of a chinese hamster ovary cell line to alkylating agents after overexpression of the human metallothionein 11-A gene Robson,T.;Hall,A.;Lohrer,H.
  18. Fundamentals of Analytical Chemistry, 6th ed. v.22 Skoog,D.A.;West,D.M.;Holler,F.J.
  19. Toxicology v.66 no.1 Effect of sodium butyrate on metallothionein induction and cadmium cytotoxicity in ROS 17/2.8 cells Thomas,D.J.;Angle,C.R.;Swanson,S.A.;Caffrey,T.C.
  20. Iarc Scientific Publications lyon. v.188 Cadmium in the hematopoietic system by cadmium in rat Waalkes,M.P.;Reham,S.;Sass,B.;Ward,J.M.
  21. Industrial Toxicology Williams,P.L.;Burson.J.L.;ed.Van Nostrand Reinhold
  22. Lead environmental health criteria 3 v.160 World Health Organization
  23. 한국자원식물 송주택
  24. 대한구강해부학회지 v.17 no.1 카드늄이 백서 배양 섬유모세포와 신경아교세포에 미치는 세포독성에 관한 연구 이용주;유현경;한두석
  25. 한국독성학회지 v.8 몇가지 중금속(Cr, Hg 및 CD)이 배양섬유 모세포에 미치는 세포독성에 관한 연구 정연태;박승택;최민규;김정중;김재민;최봉규;우원홍;위인섭
  26. 대한구강해부학회지 v.18 no.1 카드늄으로 처리한 백서 섬유 모세포와 신경아교세포에서 caffeic acid의 수복효과 한두석;김영옥;백승화