• Title/Summary/Keyword: Morphological development

Search Result 1,476, Processing Time 0.038 seconds

Birth of Calf Following Transfer of Bovine Embryos Produced by Maturation, Fertilization and Development In Vitro with Korean Native Cattle Semen (한우정액 유래 체외수정 송아지 생산에 관한 연구)

  • 황우석;조충호;이병천;신태영;노상호;김성기;전병준;이강남;신언익
    • Journal of Embryo Transfer
    • /
    • v.8 no.2
    • /
    • pp.143-149
    • /
    • 1993
  • The objective of this study was to produce calves derived from in vitro fertilization of in vitro matured follicular oocytes. Oocytes aspirated from small antral folicles of ovaries obtained at a local slaughter house were matured and fertilized in vitro. At l8hrs after insemination with Korean native cattle semen, oocytes were co-cultured for 6~7 days by utilizing co-culture system with bovine oviduct epithelial cell. After co-culture, good or excellent quality late morulae or early blastocysts were selected by morphological criteria under stereo microscope. Selected embryos were transferred to recipients on day 6 or 7 (estrus = day 0). Recipients were monitored by observation for estrus and rectal palpation after 60 days from embryo transfer. One of them went to term with the birth of a calf. This case is the first production of calf derived from in vitro fertilization in Korea.

  • PDF

New distributional record for Pyropia koreana: confirmed to occur on the South Island, New Zealand

  • Nelson, Wendy A.;Sutherland, Judith E.;Hwang, Mi Sook;Choi, Han-Gu
    • ALGAE
    • /
    • v.29 no.3
    • /
    • pp.177-181
    • /
    • 2014
  • An epiphytic bladed member of the Bangiales was found growing in Christchurch (South Island, New Zealand). Molecular sequence data and morphological comparisons revealed that the New Zealand specimens belong to the species Pyropia koreana (M. S. Hwang & I. K. Lee) M. S. Hwang, H. G. Choi, Y. S. Oh & I. K. Lee. This is the first record of Py. koreana in the southern hemisphere and a new record of an introduced species in New Zealand.

Development of X-ray Micro Computed Tomography and Applications (미세 X선 단층촬영 기법의 개발과 적용)

  • Kim, Seung-Gon;Lim, Jae-Hong;Kim, Bo-Heum;Lee, Eui-Jae;Lee, Sang-Joon
    • Journal of the Korean Society of Visualization
    • /
    • v.8 no.2
    • /
    • pp.45-50
    • /
    • 2010
  • The objective of this study is to elucidate the feasibility of synchrotron X-ray micro CT as a non-destructive imaging method to visualize the three-dimensional morphological structures of biological and non-biological samples. The experiments were conducted in 7B2 X-ray micro CT beamline in Pohang Accelerate Laboratory (PAL). A rotational 3-axis stage was specially designed for $0^{\circ}-180^{\circ}$ scanning of test samples. Preliminary tests were performed for opaque samples including a mosquito head, a plant seed and gas diffusion layer (GDL) of polymer electrolyte fuel cell to verify the feasibility of the X-ray micro CT. It visualized clearly the internal structure of all the test samples, supporting its usefulness.

Influence of Silver Ions in HA Film on Morphology of Macrophages

  • Feng, Q.L.;Kim, T.N.;Kim, J.W.
    • Journal of the Korean Vacuum Society
    • /
    • v.7 no.s1
    • /
    • pp.50-56
    • /
    • 1998
  • Ion beam assisted deposition (IBAD) was successfully used to produce a dense and ultra-adherent Hydroxyapatite (HA) film on titanium alloy and alumina. Recently it is also proved that the HA coatings on alumina substrate treated with 20 ppm $AgNO_3$ had the structure of $(Ag, Ca)_10(PO_4)6(OH)_2$, which exhibited excellent antimicrobial effects. The present paper aims to morphlogically characterize the adhesion of macrophages on newly developed Ag-HA coated alumina and Ti6A14V substrates and to evaluate the biocompatibility of the coatings in vitro. It can be found that the cell number on alumina of the concentration of $AgNO_3$ in the treatment, the cell number on Ag-HA coatings decreased. Up to 20 ppm $AgNO_3$ by Ag-treatment, the morphological development of the cells on Ag-HA coating was similar to that of the cells on HA coating, suggesting the biotolerance of the Ag-HA coatings.

  • PDF

Development of Floationg Seal Inspection System Using Line Scan Camera (라인스캔 카메라를 이용한 Floating Seal 시각 검사 시스템의 개발)

  • Park, Chang-Mok;Wang, Gi-Nam
    • Journal of the Korean Society for Precision Engineering
    • /
    • v.16 no.12
    • /
    • pp.60-70
    • /
    • 1999
  • An efficient automatic inspection system for Floating Seal is developed, The proposed system consists of a high resolution line scan camera, microcomputer, and PLC (Programmable Logic Controller). In order to perform rapid inspection, The ROI (Region of Interest) is extracted from the original image. There are types of defects; shape defects and surface defects. Each features of defects are captured by edge detect, segmentation, morphological operation, and threshold analysis. PLC controller is used to synchronize the whole system and store the inspection results temporarily to reduce the overhead of microcomputer. As a result, the system is being utilized successfully in a teal inspection line.

  • PDF

Sequential Defect Region Segmentation according to Defect Possibility in TFT-LCD Image (TFT-LCD영상에서 결함 가능성에 따른 순차적 결함영역 분할)

  • Chang, Chung Hwan;Lee, SeungMin;Park, Kil-Houm
    • Journal of Korea Multimedia Society
    • /
    • v.23 no.5
    • /
    • pp.633-640
    • /
    • 2020
  • Defect region segmentation of TFT-LCD images is performed by combining defect pixels detected by a defect detection method into defect region, or by using morphological operations to segment defect region. Therefore, the result of segmentation of the defect region is highly dependent on the defect detection result. In this paper, we propose a method which segments defect regions sequentially according to the possibility of being included in defect regions in TFT-LCD images. The proposed method repeats the process of detecting a seed using the median value and the median absolute deviation of the image, and segments the defect region using the seeded region growing method. We confirmed the superiority of the proposed method to segment defect regions using pseudo-images and real TFT-LCD images.

Double Outlet Left Ventricle - One Case Report - (양대동맥 좌심실기시증치험 1례)

  • 성후식
    • Journal of Chest Surgery
    • /
    • v.20 no.4
    • /
    • pp.798-802
    • /
    • 1987
  • Origin of both great vessels from morphological left ventricle [DOLV] is a rare cardiac anomaly which embryologic possibility has been explained by differential conal development concept and differential canal absorption concept. Recently we had surgical experience of DOLV in 4 month-age infant weighing 5.7Kg. The chief complaints on admission were cyanosis and anoxic spell during severe crying, and right heart catheterization and right ventriculogram were performed but incorrect diagnosis was made. The operative procedures were ligation of patent ductus arteriosus, patch closure of subaortic VSD aligning aorta and pulmonary artery with left ventricle, suture closure of proximal pulmonary artery and valve and the use of extracardiac valved conduit [Carpentier-Edward l4mm] from right ventricle to distal pulmonary artery. Postoperative course was uneventful and discharged in the good condition.

  • PDF

Plant Growth Promotion and Suppression of Root Disease Complex due to Meloidogyne incognita and Fusarium oxysporum by Fluorescent Pseudomonads in Tomato

  • Kumar, Tarun;Bajpai, Vivek K.;Maheshwari, Dinesh Kumar;Kang, Sun-Chul
    • Journal of Applied Biological Chemistry
    • /
    • v.48 no.2
    • /
    • pp.79-83
    • /
    • 2005
  • While screening for nematicidal activity of bacterial origins, various pseudomonads strains were inhabited in tomato rhizosphere. One isolate designated as $PE_{10}$ was selected for studies on nematicidal properties and plant growth-promoting (PGP) activity and was identified as Pseudomonas aeruginosa based on morphological features, biochemical and physiological tests, and carbohydrate utilization. To investigate nematicidal activity, Meloidogyne incognita juvenile mortality was determined using $PE_{10}$ culture filtrate. Inhibition of strain $PE_{10}$ against Fusarium oxysporum was observed using dual culture technique. Strain $PE_{10}$ showed good siderophore activity, HCN and IAA production abilities, and growth and development enhancement of tomato.

Two New Records of Marine Algae Phyllodictyon anastomosans and Saccharina ochotensis from Korea (한국산 미기록 해조 2종(Phyllodictyon anastomosans 및 Saccharina ochotensis)에 대한 주해)

  • Hwang, Mi Sook;Oh, Yoon Sik
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.46 no.1
    • /
    • pp.77-82
    • /
    • 2013
  • Phyllodictyon anastomosans (Harvey) Kraft et M.J. Wynne and Saccharina ochotensis (Miyabe) E.E. Lane C. Mayes, Druehl et G.W. Saunders are first recorded in Korea. A pantropical green alga, P. anastomosans was collected on a rock wall in the subtital zone of Jeju Island. This species has very tiny fan-shaped and netted thalli that are green in color. A cold temperate brown alga, P. ochotensis has very thick and leathery thalli that are deep brown in color. Morphological characteistics and habitats of these species are described with taxonomic accounts.

Characterization of Endogeneous Plasmids from Two Bacillus Isolates (Bacillus 속 분리균 2종의 내재형 Plasmids 특성분석)

  • 윤기홍
    • Microbiology and Biotechnology Letters
    • /
    • v.27 no.5
    • /
    • pp.364-369
    • /
    • 1999
  • In order to obtain the suitable plasmids for constructing plasmid vectors of Bacillus species, endogeneous plasmid DNAs were screended from thermo-tolerant soil bacteria. Based on agarose gel electrophoresis patterns of the isolated plasmid DNAs, two strains harboring small-size plasmids were selected. The isolated were identified to belong to the genus Bacillus on the basis of their morphological and biochemical properties, and named Bacillus sp. 3-3 and 77-8, respectively. The restriction endonuclease maps were determined for four plasmids including two plasmids from each Bacillus isolates. It is interesting that Bacillus sp. 3-3 and 77-8 have an identical plasmid according to the restriction maps. The three kinds of hybrid plasmids constructed by introducing each plasmid of two isolates into a Escherichia coli plasmid vector. pUCCm18 containing chloramplenicol resistance gene active in Bacillus strains, could be replicated in B. subtilis and B. licheniformis. These plasmids are very stable in B. subtilis, suggesting that the Bacillus plasmids identified in this work would be useful for development of new cloning vectors for Bacillus strains.

  • PDF