• Journal of the Korean Wood Science and Technology
• /
• v.44 no.6
• /
• pp.903-912
• /
• 2016

Morphological and anatomical evaluation of grafted P. merkusii have been undertaken to obtain the information about compatible and incompatible symptoms of 18 years old grafts based on morphological observation and microscopic analysis. Samples of compatible and incompatible grafts were obtained from previous research conducted by the Silviculture Departement Team in 1994. Result showed that compatible grafts have normal stem form and secondary growth (diameter growth), but some abnormality symptoms like undulated pattern of annual growth rings, phloem thickening and abnormality resin ducts in inner and middle parts of the union area occurred. Incompatible ones showed abnormality of the stem form, cortex-bark necrosis and swelling in the union area. Microscopic observation showed abnormality of all parts of the union, undulated pattern of annual growth rings, phloem thickening, abnormal resin ducts, low numbers and discontinuity of vascular elements in the union area.

• Fisheries and Aquatic Sciences
• /
• v.15 no.2
• /
• pp.117-123
• /
• 2012

The starry flounder Platichthys stellatus, like all flatfish, exhibits conspicuous lateral asymmetry in numerous traits, most obvious of which is the migration of one eye to the other side of the head during metamorphosis. Additional changes related to eye migration include asymmetrical pigmentation, and a behavioral shift from larvae that exhibit upright, open-water swimming to juveniles and adults that lie on the ocean floor, eye side up. However, the morphology of these juveniles has been quite plastic in recent years, a phenomenon which is thought to be related to a diverse suite of semi-intensive and intensive larviculture methods. The cause of morphological abnormalities in the farmed flatfish is poorly understood. In the present study, we observe the features of morphological specificity and abnormality of immature fish (mean total length 23 cm) and survey the occurrence frequency of the specificity and abnormality of juvenile (mean total length 6.70 cm) in artificial culture facility. We find 2 types of abnormality (e.g., albino in ocular side and hypermelanosis in blind side) and 1 type of specificity (e.g., lateral polymorphism). These considerably differ from normal individuals (has sinistral eye and pigmented on only one side) by several characteristics (dextral eye, ocular side albinism, blind side hypermelanosis). The incidences of albinism, hypermelanosis, and body reversal are $10.1{\pm}2.56%$, $91.7{\pm}1.7%$, and $13.1{\pm}1.1%$, respectively. These suggest that these morphometric and morphological differences occur more in artificial environment during and just after metamorphosis.

• Journal of Life Science
• /
• v.9 no.3
• /
• pp.308-313
• /
• 1999

Ocellar morphological abnormality was studied in Drosophila rdgA mutant. In the mutant, ocellar photoreceptor cells were generally affected by the defection of rdgA molecules. Among organelles of photoreceptor cell, rhabdomeres were remarkably degenerated. The rdgA molecule, diacylglycerol kinase, was localized around SRC just below the rhabdomeric region. As a secondary phenomenon of photoreceptor degeneration, rER, multivesicular body and multilamella body were appeared in cytoplasm and these were known as to clean the cellular debris. These morphological abnormality was generally observed in degenerating cells. In Drosophila mutant, the degeneration of ocellar photoreceptor cell was facilitated to time. More intense morphological defection was observed in rdgA^{ks60} rather than in yw;rdgApc47.

• Journal of Aquaculture
• /
• v.12 no.2
• /
• pp.115-122
• /
• 1999

Exposure experiments during the egg development were conducted to assess the influences of 5 different concentrations (0, 25, 50, 75 and 100%) of water-soluble fraction (WSF) of Kuwait crude oil on the eggs and larvae of black seabream (Acanthopagrus schlegeli), red seabream (Pagrus major) and olive flounder (Paralichthys olivaceus). All experiments were triplicated. Hatching time and hatching rate were examined on the eggs. The median lethal time ($LT_{50}$), morphological abnormality and swimming activity (swimming frequency and speed) of larvae were also investigated. The time and rate of egg hatching were not significantly influenced by WSF on the eggs of the fishes. The larvae exposed to WSF during the egg development were also not significantly influenced on the $LT_{50}$ and swimming activity. But the higher morphological abnormalities of notochord were observed from the larvae in 100% WSF exposure.

• Environmental Analysis Health and Toxicology
• /
• v.25 no.3
• /
• pp.197-205
• /
• 2010

The ecotoxicological effects of two antibiotics, fenbendazole and lincomycin, were observed in the harpacticoid copepod Tigriopus japonicus s.l. collected from tidal pools in the southern coast of Korea. Fenbendazole had a significant effect on the survival rates (p < 0.05), delay of copepodite emergence, and urosome size (p < 0.05). Lincomycin, on the other hand, had no significant influence on these environmental indicators. However, our analysis of morphological abnormalities in T. japonicus s.l. showed that lincomycin was more effective than fenbendazole in causing deformities. The pattern of deformity was diverse, with fused segments, and loss or addition of setae in the swimming legs. All of these patterns appeared as a result of relatively low concentrations of this antibiotic (0.3, $1\;{\mu}g\;L^{-1}$). We report here patterns of morphological abnormality in T. japonicus s.l. exposed to antibiotics, and suggest their possible application in ecotoxicological monitoring.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.56 no.6
• /
• pp.916-922
• /
• 2023

An abnormal shape of Bathyraja brachyurops was first reported from the catch of a bottom trawl in the southwest Atlantic Ocean in June 2022. Both pectoral fins of the specimen did not fuse with the head, resulting in a horn-like structure separated from the sides of the eyes. Analysis of mitochondrial DNA cytochrome c oxidase subunit I sequences showed that our specimen was perfectly matched to Bathyraja brachyurops registered with the National Center for Biotechnology Information. Our specimen possessed the following morphological features: a pair of flexible but elongated and pointed horns on the head; rough dorsal disc, densely covered with numerous small denticles on the head, anterior margin of pectoral fins and median line of the disc; a thorn between the first and second dorsal fins; and a pair of large ocelli at the base of pectoral fins. Unlike the normal B. brachyurops, our specimen had a slender clasper and no nuchal thorns, which may be related to the morphological abnormality. The horn-like structure on the head may be owing to the lack of fusion between the pectoral fins and head during early embryonic development.

• Development and Reproduction
• /
• v.23 no.1
• /
• pp.11-19
• /
• 2019

The study was conducted to investigate the effects of alpha-linolenic acid (ALA) combined with bovine serum albumin (BSA) or methyl-beta-cyclodextrin (MBCD) on plasma and acrosomal membrane damages, mitochondrial activity, morphological abnormality, motility, and oxidative stress in frozen-thawed boar sperm. In previous our study, 3 ng/mL ALA had been shown protective effect during freezing process of boar sperm. Therefore, we used 3 ng/mL ALA in present study and ALA was combined with same molar ratio of BSA or MBCD (ALA+BSA and ALA+MBCD, respectively). To confirm the effect of two carrier proteins, same volume of BSA and MBCD without ALA were added during cryopreservation. Membrane damage, mitochondrial activity, reactive oxygen species (ROS) and lipid peroxidation (LPO) levels were measured using flow cytometry, and movement of sperm tail as motility parameter and morphological abnormality were observed under light microscope. In results, all of sperm parameters were enhanced by ALA combined with BSA or MBCD compared to control groups (p<0.05). Mitochondrial activity, morphological abnormality, ROS and LPO levels in ALA+BSA or MBCD groups were no significant difference compared with ALA, BSA and MBCD treatment groups. On the other hand, plasma and acrosomal membrane intact, and sperm motility in ALA+MBCD group were higher than single treatment groups (p<0.05), whereas ALA+BSA did not differ. Our findings indicate that carrier proteins such as BSA and MBCD could improve the effect of ALA during cryopreservation of boar sperm, and treatment of ALA with carrier proteins enhance membrane integrity, mitochondrial activity through reduction of ROS-induced LPO.

• Journal of fish pathology
• /
• v.21 no.2
• /
• pp.139-148
• /
• 2008

Vertebral deformity in hatchery-reared black sea bream (Acanthoparus schlegelii) fingerlings occurred. Deformed fish had a good appetite but no clinical signs were found except the vertebral abnormality and darkness around the dorsal skin of the deformed vertebra. As more than 90% of the hatchery-reared fish exhibited vertebral abnormality, the fingerlings could not be used for commercial seeds any more. No morphological change in the swim bladder was observed. Histopathological changes on the deformed vertebra, gill, liver, and spleen were observed to clarify the cause of the deformity. The vertebra were irregularly deformed upward with the shape of "V" in an alphabetic character causing the spinal cord and dorsal aorta suppressed. The diameters of the muscle fibers around the deformed vertebra, especially, in the dorsal part, were much smaller and more irregular than those in normal fish, and the gabs between the fiber bundles were enlarged. No evidence of inflammatory responses in the lateral musculature were found. On the basis of normal inflation of the swim bladder, cultural environments for growth of the fingerlings, and histopathological alterations in the muscle, vertebra and gills, it is suggested that high speed of water current in the culturing aquaria between 23 to 30 days after hatching was responsible for the development of vertebral abnormality.

• Journal of fish pathology
• /
• v.20 no.2
• /
• pp.129-137
• /
• 2007

Skeletal deformity in hatchery-reared river puffer (Takifugu obscurus) fingerlings occurred. The vertebral abnormality was recognized with naked eyes 80 days after hatching. Affected fish had a good appetite but no clinical signs were found except the vertebral abnormality. As more than 90% of the hatchery-reared fish exhibited vertebral abnormality, the fingerlings could not be used for commercial seeds any more. Morphological changes in the vertebrate and the swim bladder were observed with a soft X-ray. Histopathological changes on the caudal muscle around the deformed vertebrae, gill, and spleen were also examined to clarify the cause of the deformity. Spinal curvature occurred between vertebrae 10 and 12, but any malfunction of the swim bladder was not found. The diameters of the muscle fibers around the deformed vertebrae were much smaller and more irregular than those in normal fish, and the gabs between the fiber bundles were greatly enlarged. No evidence of inflammation response was found in the muscle layer. In the hatchery, feed was putting at the position of water inflow, which might attract the fingerings to move toward the feed in spite of incomplete development of their caudal muscle fibers. From these results, it is suggested that the high speed of water current in rearing aquaria might be associated with the development of vertebral abnormality.

• Journal of Korean Society on Water Environment
• /
• v.33 no.4
• /
• pp.424-433
• /
• 2017

The purpose of this study is to understand the changes on species composition of the epilithic diatoms and the appearance of morphological abnormalities in the upper region of the Nakdong River where heavy metal inflow is observed. The samples were collected once a week for a month of September 2016 from selecting 7 sampling stations. The heavy metals of cadmium (Cd) and arsenic (As) were not detected during the survey period, but zinc (Zn) was detected in st.4 - st.6 with the range of $0.015{\sim}0.188mg\;L^{-1}$. Metal sensitive species such as Achnanthes convergens, Cocconeis placentula, Cocconeis placentula var. euglypta, Cocconeis placentula var. lineata showed high dominance in st.1 - st.3, st.7. However, metal tolerant species such as Nitzschia palea, Achnanthes minutissima showed high dominance in st.4 - st.6. It is concluded that heavy metal inflow directly affects the changes in species composition of epilithic diatoms. As a result of CCA, the characteristics of the sampling sites were divided into 3 groups. Group 1 was represented the non-detected Zn sites with C. placentula, C. placentula var. euglypta, C. placentula var. lineata. Group 2 was showed the detected Zn sites with Navicula minima and Nitzschia palea. Group 3 was included st.3 - st.7 on 4th week that was stabilized the community structure. Total 8 taxa of abnormal frustules observed. This occurrence of abnormal frustules reflected the temporal and quantitative indicators of heavy metal pollution, in particular, it was confirmed that genus Fragilaria, which has a high abnormality according to heavy metal pollution, can be used as an indicator species.