Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지

Vertebral abnormality in Hatchery-reared River puffer, Takifugu obscurus fingerlings

인공종묘 생산 황복 (Takifugu obscurus) 치어의 척추 변형에 관하여

  • Park, Sung-Woo (Department of Aquatic life Medicine, Kunsan National University) ;
  • Kang, Kook-Hee (Department of Aquatic life Medicine, Kunsan National University)
  • 박성우 (군산대학교 수산생명의학과) ;
  • 강국희 (군산대학교 수산생명의학과)
  • Published : 2007.08.30
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Abstract

Skeletal deformity in hatchery-reared river puffer (Takifugu obscurus) fingerlings occurred. The vertebral abnormality was recognized with naked eyes 80 days after hatching. Affected fish had a good appetite but no clinical signs were found except the vertebral abnormality. As more than 90% of the hatchery-reared fish exhibited vertebral abnormality, the fingerlings could not be used for commercial seeds any more. Morphological changes in the vertebrate and the swim bladder were observed with a soft X-ray. Histopathological changes on the caudal muscle around the deformed vertebrae, gill, and spleen were also examined to clarify the cause of the deformity. Spinal curvature occurred between vertebrae 10 and 12, but any malfunction of the swim bladder was not found. The diameters of the muscle fibers around the deformed vertebrae were much smaller and more irregular than those in normal fish, and the gabs between the fiber bundles were greatly enlarged. No evidence of inflammation response was found in the muscle layer. In the hatchery, feed was putting at the position of water inflow, which might attract the fingerings to move toward the feed in spite of incomplete development of their caudal muscle fibers. From these results, it is suggested that the high speed of water current in rearing aquaria might be associated with the development of vertebral abnormality.

인공 종묘 생산한 황복 (Takifugu obscurus)자어의 사육 중에 발생한 척추 변형의 원인을 조사하기 위하여 변형어를 병리조직학적으로 조사하였다. 척추 변형은 부화 후 80일령의 황복으로 변형의 발생율은 전체 사육 미수의 약 90%였다. 변형어를 soft-X ray로 촬영한 결과 부레의 팽창은 정상적이었으며, 병리조직학적으로는 변형이 발생한 척추 주변의 근섬유 다발이 소형화되면서 모양도 불규칙하게 변하였다. 그러나 새변 연골은 뒤틀림이나 증생 등은 관찰되지 않았다. 척추 변형이 발생한 종묘 생산장에서는 일반적인 사육 방식보다 1-2일 정도 사육수를 빨리 순환시키면서 급이하고 있었는데, 그 후 황복이 무리를 형성하고 나서, 급이하면서 사육수를 환수한 결과 변형의 발생은 없었다. 병어의 조직학적 변화를 사육방식과 더불어 고찰하였을 때, 유영 능력이 불완전한 황복에 급이하면서 사육수를 환수시킴으로써 황복이 먹이를 섭취하기 위하여 수류에 저항하면서 유영한 것이 척추 변형의 원인으로 작용하였을 것으로 생각한다.

Keywords

References

  1. Backiel, T., Kokurewicz, B.. and Ogorzalek, A.: Highincidence of skeletal anomalies in carp, Cyprinus carpio, reared in cages in flowing water. Aquaculture, 43:369-380, 1984 https://doi.org/10.1016/0044-8486(84)90245-X
  2. Bengtsson, B.-E. and Larsson, A.: Vertebral defor mities and physiological effects in fourhom sculpin (Myoxocephalus quadricornis) after long-term exposure to a simulated heavy metal containing effluent. Aquat. Toxicol., 9: 215-229, 1986 https://doi.org/10.1016/0166-445X(86)90010-X
  3. Cahtain, B.: The swim bladder in Dicentrachus labrax and Sparus auratus. I. Morphological aspects of development. Aquaculture, 53: 303-311, 1986 https://doi.org/10.1016/0044-8486(86)90361-3
  4. Cahtain, B.: Abnormal swim bladder development and lordosis in sea bass (Dicentrachus labrax) and sea bream (Sparus auratus). Aquaculture, 119: 371-379, 1994 https://doi.org/10.1016/0044-8486(94)90301-8
  5. Chatain, B. and Ounasis-Guschemann, N.: Improved rate of initial swim bladder inflation in intensively reared Sparus auratus. Aquaculture, 84: 345-353, 1990 https://doi.org/10.1016/0044-8486(90)90099-9
  6. Choi, S.D., Hong, S.Y. and Park, K.J.: Two species of parasitic copepodes (Clavellopsis hugu and Taeniacanthus yamagutii) from the cultured marine fish, from the western coast of Korea. J. Fish Pathol., 11 (2): 119-127, 1998
  7. Divanch, P., Papandroulakis, N., Anastasisadis, P., Koumoundouros, G. and Kentouri, M.: Effect of water currents on the development of skeletal deformities in sea bass (Dicentrarchus labrax L.) with functional swim bladder during postlarval and nursery phase. Aquaculture, 156: 145-155, 1997 https://doi.org/10.1016/S0044-8486(97)00072-0
  8. Harver, J.E., Ashley, L.M. and Smith, R.R.: Ascorbic acid requirement of coho salmon and rainbow trout. Trans. Am. Fish. Soc., 98: 762-771,1969 https://doi.org/10.1577/1548-8659(1969)98[762:AAROCS]2.0.CO;2
  9. Kihara, M., Ogata, S., Kawano, N., Kubota, I., and Yamaguchi, R.: Lordosis induction in juvenile red sea bream Pargus major, by high swimming activity. Aquaculture, 212: 149-158, 2002 https://doi.org/10.1016/S0044-8486(01)00871-7
  10. Kitajima, C., Tsukashima, Y., Fujita S., Watanabe, T. and Yone, Y. : Relationship between uninflated swim bladders and lordotic deformity in hatchery-reared red sea bream Pargus major. Bull. Jap. Soc. Sci. Fish., 47: 12891294,1981 https://doi.org/10.2331/suisan.47.1289
  11. Kitamura, S., Ohara, S., Suwa, T. and Nakagawa, T.: Studies on vitamin requirements of rainbow trout, Salmo gairdneri -1. On the ascorbic acid. Bull. Jap. Soc. Sci. Fish., 31: 818-826,1965 https://doi.org/10.2331/suisan.31.818
  12. KOROl: Development of technique on the seeding mass production and propagation of river puffer, Takifugu obscurus. BSPG 00225876-3. Minst. Agricul. Fish., Seoul, pp 244, 1995
  13. Lim, C. and Lovell, R.T.: Pathology of the vitamin C deficiency syndrome in channel catfish (Jctalurus punctatus). J. Nutri., 108: 1137-1146, 1978 https://doi.org/10.1093/jn/108.7.1137
  14. Ransom, D.R., Nannam, C.N., Rohovec, J.S. and Flyer, J.L.: Comparison of histopathology caused Vibrio anguillraum and Vibrio ordalii in the species of Pacific salmon. J. Fish Dis., 7: 107-115, 1984 https://doi.org/10.1111/j.1365-2761.1984.tb00913.x
  15. Sakaguchi, S., Hara, T., Matsusata, T., Shibara, T., Yamguchi, Y., Kawai, H., and Maeno, Y.: Scoliosis of cultured yellowtail caused by parasitic Myxobolus burl. Bull. Natl, Inst. Aquacult., 12: 79-86, 1987
  16. Takashima, F.: Vertebral malformation in hatcheryreared red sea bream, Chrysophrys major. Bull. Jap. Soc. Sci. Fish., 44: 435-443, 1978 https://doi.org/10.2331/suisan.44.435
  17. Weppe, M. and Bonami, J.R.: Non inflation of the swim bladder in hatchery reared sea bass and sea bream: a significant problem in marine aquaculture. Bull. Eur. Assoc. Fish Pathol., 3: 59-60, 1983
  18. Yamashita, K.: Functional studies for the culture of Chrysophrys major. IV. On disease of larval and young fish (2). Abnormal expansion of the swim bladder. Bull. Jap. Soc. Sci. Fish., 32: 1006-1014, 1966 https://doi.org/10.2331/suisan.32.1006
  19. Yukio M., Sorimachi, M., Ogawa, K. and Egusa, S.: Myxobolus spinacurvatura sp. n. (Myxosporea: Bivalvulida) parasitic in deformed mullet, Mugil cephalus. Fish Pathol., 25: 37-41,1990 https://doi.org/10.3147/jsfp.25.37
  20. 박성우, 최현민, 유진하: 해수 양식 황복 (Takifugu obscurus)의 백점충(Crytocaryon irritans)감염. 한국어병학회지, 17: 99-103, 2004
  21. 방종득: 담수양식 해산어류의 질병 방제 연구. 해산어 담수양식 세미나. 국립수산진흥원 청평 내수면 연구소. pp 111-123, 2001
  22. 정문기:한국어도보, 일지사, pp 605-606, 1991
  23. 장선일, 강희웅, 한형균: 황복의 난발생과 자치어의 발달. 한국양식학회지, 9:11-18, 1996