• Title/Summary/Keyword: Moon Jae-in

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Degradation of Clavulanic Acid During the Cultivation of Streptomyces clavuligerus; Instability of Clavulanic Acid by Metabolites and Proteins from the Strain

  • Ishida Kenji;Hung Trinh Viet;Lee Hei-Chan;Liou Kwang-Kyoung;Shin Chang-Hun;Yoon Yeo-Joon;Sohng Jae-Kyung
    • Journal of Microbiology and Biotechnology
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    • v.16 no.4
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    • pp.590-596
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    • 2006
  • Clavulanic acid (CA) produced by Streptomyces clavuligerus is degraded during the bacterial cultivation. The degradation was examined in three different aspects, including physical, chemical, and enzymatic effects, in order to understand the degradation during the cultivation. The result showed that CA was unstable in the production medium containing ammonium salts and amino acids, owing to ammonium ions and amine groups. In addition, the degradation was not only due to instability of CA by metabolites and proteins, but also enzymes from S. clavuligerus such as $\beta-lactamase$ and penicillin-binding proteins. However, the degradation caused by these enzymes was not highly significant compared with the degradation during the cultivation, owing to irreversible reactions between CA and enzymes.

A Study of the Transition Process in Presidential Electronic Records Transfer and Improvement Measures : Focused on the Electronic Records of the 19th President Moon Jae-in's Administration (대통령 전자기록물의 이관방식 변천과 개선방안 연구 19대 문재인 정부 대통령 전자기록물을 중심으로 )

  • Yun, Jeonghun
    • The Korean Journal of Archival Studies
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    • no.75
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    • pp.41-89
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    • 2023
  • Since the enactment of the Act on the Management of Presidential Archives in 2007, the cases of electronic records transfer in the 16th President Roh Moo-hyun's administration have played the role of an advance guard in managing public records and served as a test bed for new electronic records management. When transferring the electronic records of the 19th President Moon Jae-in's administration, the electronic records transfer method of President Roh's administration was inherited, while several innovative attempts were made. For instance, the Presidential Archives have for the first time converted the electronic documents from institutions advising the President into a long-term preservation package and transferred them online. In addition, considering the characteristics of the data, the administrative information dataset of the Presidential record creation institutions was transferred to the SIARD standard. Furthermore, the Presidential Archives had websites transferred in the form of OVF as a pilot test and collected social media directly through the API. Thus this study investigated the transition process of the presidential electronic records transfers from the 16th President Roh Moo-hyun's administration to the 19th President Moon Jae-in's. In addition, major achievements and issues were analyzed centering on the transfer method by type of electronic records during President Moon Jae-in's administration, and future improvement plans were presented.

The Change of Streetscape in Hong-Tong Street (홍통거리 가로경관 변화과정)

  • Koo, Yun-Ho;Ban, Jae-Sick;Jung, Yeon-Seo;Lee, Hee-Won
    • Proceedings of the KAIS Fall Conference
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    • 2010.05b
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    • pp.818-821
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    • 2010
  • 본 논문에서는 홍통거리의 신축 건물과 리모델링 건물을 통한 재생 건축의 조화의 방법에 대해 모색해 보고, 해결방안을 제시해 보도록 한다. 현재 홍통거리는 거리의 발전에 따라 오래된 건축물들을 리모델링한 건물과 신축건물들이 생겨남에 따라, 두 건물과의 조화된 모습을 찾고자 한다. 조화된 거리의 모습을 이루기 위해 거리 바닥 패턴과 일관된 시설물을 통해 거리의 형성을 해나가야겠다.

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One-pot Enzymatic Synthesis of UDP-D-glucose from UMP and Glucose-1-phosphate Using an ATP Regeneration System

  • Lee, Hei-Chan;Lee, Seung-Don;Sohng, Jae-Kyung;Liou, Kwang-Kyoung
    • BMB Reports
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    • v.37 no.4
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    • pp.503-506
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    • 2004
  • Glucose-1-phosphate uridylyltransferase from E. coli K12 was used to convert uridine-5'-triphosphate and glucose-1-phosphate to UDP-D-glucose. The conversion was efficient and completed within 5 minutes under the employed conditions. In addition, thymidine-5'-monophosphate kinase and acetate kinase were proven to be non-specific, converting udridine-5'-monophosphate to uridine-5'-triphosphate with 55% conversion after 6 h, which was much slower than the production of TTP under the same conditions (complete conversion within one hour). Since these two reactions could proceed under the same conditions, a one-pot synthesis of UDP-D-glucose with ATP regeneration was designed from easily available starting materials, and conversion up to 40% by HPLC peak integration was achieved given a reaction time of 4 h.

Design of the Moving Appliance for Disabled Persons Supporting Getting in the Car (휠체어 사용자의 차량탑승 지원을 위한 이동기구설계)

  • Oh, Keon-Tack;Kim, Ki-Sik;Jung, Jae-Hyun;Kim, Sung-Ki
    • Proceedings of the Korea Information Processing Society Conference
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    • 2014.11a
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    • pp.1211-1212
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    • 2014
  • 본 논문에서는 차종에 관계없이 휴대가 가능하며 사용자가 휠체어에서 차량의 좌석으로 이동시키는 로봇팔을 설계하고 경제성과 이동성, 휴대용이성이라는 사용자요구를 충족할 것이다.

Design of a Lifting Appliance to Overcome Lifting Problem in Working Places with Space Limit (공간접근성 제약을 극복하는 들어올림 기구설계)

  • Jung, Jae-Hyun;Park, Dong-Yeon;Ko, Kuk-Won;Kim, Sung-Ki
    • Proceedings of the Korea Information Processing Society Conference
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    • 2014.11a
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    • pp.1213-1214
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    • 2014
  • 일상에서 좁은 공간에 있는 무거운 물건을 쉽고 편리하게 들어 올리거나 이동시키는 들어 올림 기구의 수요가 있다. 본 연구에서는 일상에서 공간의 제약을 극복하고, 무거운 물건을 들어 올려, 다양한 작업 용용에 활동하는 기구를 설계하고, 3차원 컴퓨터 설계 모형을 제시한다.