• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2000년도 영호남학술대회 논문집
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• pp.265-268
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• 2000

We fabricated an IMI-O polymer containing an imidazole group that could form a complex structure between the monolayer and the metal ions at the air-water interface. Also. the monolayer behavior at the air-water interface and the surface morphology of metal-complexed Langmuir-Blodgett(LB) films were investigated by using Brewster angle microscopy(BAM) and Scanning Maxwell-stress Microscopy(SMM). The difference in the BAM and SMM images between the pure water and the aqueous metal ions is attributed to the interactions of the copolymers with the metal ions at the interface and the consequent change of the monolayer organization.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1998년도 추계학술대회 논문집 학회본부 C
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• pp.881-883
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• 1998

We fabricated IMI-O polymer containing imidazole group that can be form a complex structure between the monolayer and the metal ions at the air-water interface. Also, the monolayer behavior at the air-water interface and Langmuir-Blodget films by complexes formation have been investigated by $\pi$-A isotherms, Brewster Angle Microscopy and the scanning Maxwell-stress microscopy.

• BMB Reports
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• 제53권2호
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• pp.74-81
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• 2020

Under physiological and pathological conditions, mechanical forces generated from cells themselves or transmitted from extracellular matrix (ECM) through focal adhesions (FAs) and adherens junctions (AJs) are known to play a significant role in regulating various cell behaviors. Substantial progresses have been made in the field of mechanobiology towards novel methods to understand how cells are able to sense and adapt to these mechanical forces over the years. To address these issues, this review will discuss recent advancements of traction force microscopy (TFM), intracellular force microscopy (IFM), and monolayer stress microscopy (MSM) to measure multiple aspects of cellular forces exerted by cells at cell-ECM and cell-cell junctional intracellular interfaces. We will also highlight how these methods can elucidate the roles of mechanical forces at interfaces of cell-cell/cell-ECM in regulating various cellular functions.

• 한국가시화정보학회지
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• 제13권1호
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• pp.43-48
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• 2015

Collective cell migration is a fundamental phenomenon observed in various biological processes such as development, wound healing, and cancer metastasis. During the collective migration, cells undergo changes in their phenotypes from those of stable to the migratory state via the process called epithelial-mesenchymal transition (EMT). Recent findings in biology and biochemistry have shown that EMT is closely related to the cancer invasion or metastasis, but not much of the correlations in kinematics and physical forces between the neighboring cells are known yet. In this study, we aim to understand the cell migration and stress distribution within the expanding cell cluster. We constructed the in vitro cell cluster on the hydrogel, employed traction force microscopy (TFM) and monolayer stress microscopy (MSM) to visualize the physical forces within the expanding cell monolayer. During the expansion, cells at the cluster edge exhibited enhanced motility and developed focal adhesions that are the essential features of EMT while cells at the core of the cluster maintained the epithelial characteristics. In the aspect of mechanical stress, the cluster edge had the highest traction force of ~90 Pa directed toward the cluster core, which means that cells at the edge actively pull the substrate to make the cluster expansion. The cluster core of the tightly confined cells by neighboring cells had a lower traction force value (~60 Pa) but the highest intercellular normal stress of ~800 Pa because of the accumulation of traction from the edge of the monolayer.

• 한국가시화정보학회지
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• 제20권2호
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• pp.70-77
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• 2022

Skeletal muscle tissues feature cellular heterogeneity, including differentiated myofibers, myoblasts, and satellite cells. Thanks to the presence of undifferentiated myoblasts and satellite cells, skeletal muscle tissues can self-regenerate after injury. In skeletal muscle regeneration, the collective motions among these cell types must play a significant role, but little is known about the dynamic collective behavior during the regeneration. In this study, we constructed in vitro platform to visualize the migration behavior of skeletal muscle cells in specific conditions that mimic the biochemical environment of injured skeletal muscles. We then visualized the spatiotemporal distribution of stresses arising from the differential collectiveness in the cellular clusters under different conditions. From these analyses, we identified that the heterogeneous population of muscle cells exhibited distinct collective migration patterns in the injury-mimicking condition, suggesting selective activation of a specific cell type by the biochemical cues from the injured skeletal muscles.

• Journal of Korean Vacuum Science & Technology
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• 제4권4호
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• pp.102-106
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• 2000

We report scanning force microscope (SFM) observations of enhanced calcite dissolution in aqueous solution due to mechanical stimulation induced by the SFM tip. Images and mechanical treatment were performed in saturated ($\geq$ 60 ${\mu}{\textrm}{m}$) CaCO$_3$ solution adjusted to pH~9. Small area scans of monolayer steps significantly increased the step velocity in the scanned area (in the direction corresponding to dissolution) when the applied contact force is above about 160 nN fer the tips employed. The step velocity could be increased at least an order of magnitude by scanning at even higher contact forces (e.g.,270nN). This enhancement is a function of step orientation relative to the calcite lattice. Indentations near preexisting steps also locally enhance the step velocity. We present evidence that the higher dissolution rates are caused by stress-induced increases in the rate of double-kink nucleation.